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miRNA-21-5p is an important contributor to the promotion of injured peripheral nerve regeneration using hypoxia-pretreated bone marrow-derived neural crest cells

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Neural Regeneration Research 2025年第1期20卷 277-290页

作者： Meng Cong Jing-Jing Hu yan Yu Xiao-li li Xiao-Ting Sun li-Ting Wang Xia Wu ling-Jie Zhu Xiao-Jia yang Qian-Ru He Fei Ding Hai-yan Shi School of Medicine Nantong UniversityNantongJiangsu ProvinceChina Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education and Coinnovation Center of Neuroregeneration Nantong UniversityNantongJiangsu ProvinceChina Department of Physiology Jiangsu Health Vocational CollegeNanjingJiangsu ProvinceChina Department of Pathology Affiliated Hospital of Nantong UniversityNantongJiangsu ProvinceChina Jiangsu Clinical Medicine Center of Tissue Engineering and Nerve Injury Repair Affiliated Hospital of Nantong UniversityNantongJiangsu ProvinceChina

Our previous study found that rat bone marrow–derived neural crest cells(acting as Schwann cell progenitors)have the potential to promote long-distance nerve ***-based therapy can enhance peripheral nerve repair and regeneration through paracrine bioactive factors and intercellular ***,the complex contributions of various types of soluble cytokines and extracellular vesicle cargos to the secretome remain *** investigate the role of the secretome and extracellular vesicles in repairing damaged peripheral nerves,we collected conditioned culture medium from hypoxia-pretreated neural crest cells,and found that it significantly promoted the repair of sensory neurons damaged by oxygen-glucose *** mRNA expression of trophic factors was highly expressed in hypoxia-pretreated neural crest *** performed RNA sequencing and bioinformatics analysis and found that miR-21-5p was enriched in hypoxia-pretreated extracellular vesicles of neural crest ***,to further clarify the role of hypoxia-pretreated neural crest cell extracellular vesicles rich in miR-21-5p in axonal growth and regeneration of sensory neurons,we used a microfluidic axonal dissociation model of sensory neurons in vitro,and found that hypoxia-pretreated neural crest cell extracellular vesicles promoted axonal growth and regeneration of sensory neurons,which was greatly dependent on loaded ***,we constructed a miR-21-5p-loaded neural conduit to repair the sciatic nerve defect in rats and found that the motor and sensory functions of injured rat hind limb,as well as muscle tissue morphology of the hind limbs,were obviously *** findings suggest that hypoxia-pretreated neural crest extracellular vesicles are natural nanoparticles rich in ***-21-5p is one of the main contributors to promoting nerve regeneration by the neural crest cell *** helps to explain the mechanism of action of the secretome and e

关键词： axotomy cell-free therapy conditioned medium extracellular vesicles hypoxic preconditioning microRNA oxygen-glucose deprivation peripheral nerve injury Schwann cell precursors

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谈课堂教学中的“师生互动”

读与写(教育教学刊) 2009年第5期6卷 69-70+3页

作者：李衍妮青岛农业大学人文社科学院

课堂中的师生互动,随着课改的不断深入大家越来越注意到了它的重要性,本文在简单地介绍了国内外的相关研究成果的基础上,探讨了在教学实践中,如何进行师生互动,如何提高师生互动效果。

关键词：师生互动课堂教学

Zn-Fe LDHs改性石英砂生物膜体系对BDE-47的去除效果与机理

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水生生物学报 2023年第8期47卷 1293-1300页

作者：蔡勤李燕妮王畅王亚芬中国地质大学(武汉)环境学院武汉430074 湖北省水环境污染控制和治理工程技术研究中心武汉430074

文章以四溴联苯醚(BDE-47)为目标污染物,利用共沉淀法制备Zn-Fe LDHs覆膜改性石英砂基质,在好氧、厌氧及两者交替条件下,研究腐败希瓦氏菌CN32(Shewanella putrefaciens CN32)在LDHs改性基质上生物膜形成过程及其对培养液中BDE-47的去... 详细信息

文章以四溴联苯醚(BDE-47)为目标污染物,利用共沉淀法制备Zn-Fe LDHs覆膜改性石英砂基质,在好氧、厌氧及两者交替条件下,研究腐败希瓦氏菌CN32(Shewanella putrefaciens CN32)在LDHs改性基质上生物膜形成过程及其对培养液中BDE-47的去除效果;通过监测反应体系中Fe^(2+)和H_(2)O_(2)浓度变化探讨BDE-47的生物及非生物去除机制。结果表明,LDHs改性不影响石英砂基质表面生物膜的形成,但在好氧条件下,Zn-Fe LDHs石英砂改性基质对CN32电子传递链活性存在一定抑制作用,而在厌氧条件下,LDHs改性会影响基质生物膜胞外聚合物(EPS)组成特性,使多糖占比升高。无论在好氧还是厌氧条件下,基质生物膜反应体系中EPS总浓度均显著高于纯菌CN32体系;且在好氧与交替条件下,基质生物膜的形成均显著提高反应体系中BDE-47的去除效果(约25%)。在交替条件下,前3次循环(72h内)BDE-47的去除以基质吸附为主;72h后,生物膜吸附与生物降解共同发挥作用,且LDHs改性基质在后期上升潜力更大。研究报道了LDHs改性基质生物膜形成特性及其对水相中PBDEs去除的潜力,为强化人工湿地中PBDEs生物降解提供新思路。

关键词：多溴联苯醚吸附生物降解生物膜希瓦氏菌

离合词“连”字句离析形式的特征、生成机制及语用功能

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语言与翻译 2015年第3期 5-8页

作者：李衍妮青岛农业大学人文社科学院青岛266109 中国传媒大学文学院北京100024

离合词有两种相对应的表达形式,即"离"的形式与"合"的形式,在实际运用中,两种形式呈现出不同的表达效果和语用功能。文章着重讨论了现代汉语离合词"连"字句离析形式的特征、生成机制及语用功能。

关键词：离合词 “连”字句离析形式语用

新时代革命文化资源在高校思政课中的有效运用

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湖北开放职业学院学报 2022年第20期35卷 81-83页

作者：李嫣妮西京学院马克思主义学院陕西西安710123

新时代革命文化资源包含着丰富的物质文化资源与精神文化资源,它是引领高校思政课政治方向、充实高校思政课教学内容及提升高校思政课育人效果的优质资源。然而,在运用过程中仍存在着革命文化资源的内容选择不精准、内容讲解不专业及运... 详细信息

新时代革命文化资源包含着丰富的物质文化资源与精神文化资源,它是引领高校思政课政治方向、充实高校思政课教学内容及提升高校思政课育人效果的优质资源。然而,在运用过程中仍存在着革命文化资源的内容选择不精准、内容讲解不专业及运用成效不够显著的困境,因而需要进一步探索革命文化资源在高校思政课中的有效运用路径。

关键词：革命文化资源高校思政课有效方法

基于核酸分子光开关的闭管可视化环介导等温扩增检测方法

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分析测试学报 2022年第4期41卷 646-651页

作者：王芳董菁李艳妮徐秦峰陕西科技大学食品与生物工程学院陕西西安710021

该研究采用核酸分子“光开关”[Ru(bpy)_(2)(dppz)]^(2+)作为环介导等温扩增(LAMP)荧光染料,建立了一种快速、直观的闭管可视化LAMP检测方法。为避免高浓度染料对LAMP反应造成的强烈抑制和开盖导致的气溶胶交叉污染,该文采用蜡封反应管... 详细信息

该研究采用核酸分子“光开关”[Ru(bpy)_(2)(dppz)]^(2+)作为环介导等温扩增(LAMP)荧光染料,建立了一种快速、直观的闭管可视化LAMP检测方法。为避免高浓度染料对LAMP反应造成的强烈抑制和开盖导致的气溶胶交叉污染,该文采用蜡封反应管进行检测,通过直接观察扩增产物DNA与[Ru(bpy)_(2)(dppz)]^(2+)结合后产生的强烈红色荧光信号判定结果。该方法对金黄色葡萄球菌DNA的检出限可低至20拷贝/反应。方法特异性强,整个可视化检测过程可在1 h内完成,有望广泛应用于环境检测、食品安全、临床诊断等领域的现场快速核酸检测。

关键词：环介导等温扩增法(LAMP) [Ru(bpy)_(2)(dppz)]^(2+) 可视化检测金黄色葡萄球菌

Insights into spinal muscular atrophy from molecular biomarkers

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Neural Regeneration Research 2025年第7期20卷 1849-1863页

作者： Xiaodong Xing Xinzhu liu Xiandeng li Mi li Xian Wu Xiaohui Huang Ajing Xu yan liu Jian Zhang Department of Clinical Pharmacy Xinhua HospitalShanghai Jiao Tong University School of MedicineShanghaiChina College of Pharmacy Chongqing Medical UniversityChongqingChina

Spinal muscular atrophy is a devastating motor neuron disease characterized by severe cases of fatal muscle *** is one of the most common genetic causes of mortality among infants aged less than 2 *** research is currently receiving more attention,and new candidate biomarkers are constantly being *** review initially discusses the evaluation methods commonly used in clinical practice while briefly outlining their respective pros and *** also describe recent advancements in research and the clinical significance of molecular biomarkers for spinal muscular atrophy,which are classified as either specific or non-specific *** review provides new insights into the pathogenesis of spinal muscular atrophy,the mechanism of biomarkers in response to drug-modified therapies,the selection of biomarker candidates,and would promote the development of future ***,the successful utilization of biomarkers may facilitate the implementation of gene-targeting treatments for patients with spinal muscular atrophy.

关键词： biomarkers disease progression gene-targeting therapy neurofilaments Nusinersen spinal muscular atrophy(SMA) survival motor neuron therapeutic evaluation treatment outcomes

徐小斌小说的生态关怀——以圆形叙事为考察视角

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海南师范大学学报（社会科学版） 2022年第3期35卷 42-50,86页

作者：李燕妮上海交通大学人文学院中文系上海200240

徐小斌的《海火》《羽蛇》《炼狱之花》等作品均体现了鲜明的生态关怀,而此种生态关怀多在其小说创作的圆形叙事技法中彰显出来。圆形叙事作为一种循回复沓、层进迭升的叙事技法,以其结构特性暗合自然、人事、社会的螺旋式发展规律。徐... 详细信息

徐小斌的《海火》《羽蛇》《炼狱之花》等作品均体现了鲜明的生态关怀,而此种生态关怀多在其小说创作的圆形叙事技法中彰显出来。圆形叙事作为一种循回复沓、层进迭升的叙事技法,以其结构特性暗合自然、人事、社会的螺旋式发展规律。徐小斌小说中生态关怀的彰显,主要借力于其小说中的圆形时间叙事模式及圆形空间叙事模式,从圆形叙事技法入手来剖析徐小斌小说中的生态关怀,不仅将为考察徐小斌小说中的生态批判精神提供渠道,还将揭示徐小斌小说中圆形叙事技法的独特生态价值,同时亦将为当代文学中的生态批评提供了新的考察视角。

关键词：徐小斌圆形叙事生态关怀圆形时间圆形空间

Manufacture the conducting polymer with light

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Science China Chemistry 2025年第01期 8-9页

作者： yang li Tianyu li yan Ji Baoguang liu College of Electronic and Optical Engineering & College of Flexible Electronics (Future Technology) Nanjing University of Posts & Telecommunications

Poly(3,4-ethylenedioxythiophene) (PEDOT), heralded as the “king” of conducting polymers, is esteemed in bioelectronics for its mixed electronic and ionic conductivity, mechanical properties compatible with biological tissues, tunable healing ability and stability in diverse environments [1]. Based on additive manufacturing of three-dimensional structures,PEDOT realizes the implementation in the efficient production of flexible bioelectronics, the construction of intricate electrode-neural implantations, and the development of smart brain-computer-interfaces [2].

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