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社区教育体验式学习模式的实践与探索——以广州市越秀区为例

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广州城市职业学院学报 2024年第1期18卷 15-19页

作者：赖晓凤张东胜李红伟中共广州市越秀区委党校广东广州510000

社区教育体验式学习模式是国内外丰富的体验学习理论在社区教育中的应用和实践,具有主体性、情境性、合作性的特征,符合社区教育高质量发展的必然要求,有助于进一步优化社区教育资源整合利用,促进社区教育特色发展,服务社区治理能力的... 详细信息

社区教育体验式学习模式是国内外丰富的体验学习理论在社区教育中的应用和实践,具有主体性、情境性、合作性的特征,符合社区教育高质量发展的必然要求,有助于进一步优化社区教育资源整合利用,促进社区教育特色发展,服务社区治理能力的提升。广州市越秀区以体验学习理论指导社区教育工作,积极探索多维度体验式学习模式的实践路径,通过建立资源共享型的体验式学习基地、打造地域特色型的体验式项目、开发资源辐射型的体验式学习圈、培育群体共学型的体验式社区学习共同体、搭建网络互动型的体验式学习平台,促进社区教育体验式学习的可持续发展,有效推动社区教育高质量发展。

关键词：社区教育体验式学习学习模式

Hemo oxygenase-1 induction in vitro and in vivo can yield pancreas islet xenograft survival and improve islet function

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Chinese Medical Journal 2011年第20期124卷 3378-3385页

作者： CHEN Xi SU Chang ZHANG Zheng-yun ZHANG Ming-jun GU wei-qiong li Xiao-ying li hong-wei ZHOU Guang-wen Department of Surgery Ruijin Hospital Shanghai Jiao Tong University School of Medicine Shanghai 200025 China Department of Endocrine and Metabolism Ruijin Hospital Shanghai Jiao Tong University School of Medicine Shanghai 200025 China Department of Surgery Shanghai First Hospital Shanghai Jiao Tong University School of Medicine Shanghai 200080 China

Background The induced expression of heme oxygenase-1 （HO-1） in donor islets improves allograft survival. Cobalt protoporphyrin （CoPP） could significantly enhance the expression of HO-1 mRNA and protein in rat islet safely. Our work was to study how to protect pancreatic islet xenograft by CoPP-induction. Methods Islet xenografts treated with CoPP-induction and CoPP＋ Zinc protoporphyrin （ZnPP） in vitro and in vivo were randomly transplanted into murine subrenal capsule; then the graft survival time was compared by blood glucose level and pathological examination and meanwhile the interferon ~ （IFN-y）, tumor necrosis factor a （TNF-a）, interleukin 10 （IL-10） and IL-113 level in serum and their mRNA and HO-1 mRNA and protein expression were examined. Results Islets with CoPP-induction under low- and high-glucose stimulation exhibited much higher insulin secretion compared with other three groups. CoPP-induction could increase higher expression of HO-1 （mRNA： 3.33- and 76.09- fold in vitro and in vivo; protein： 2.85- and 58.72-fold）. The normoglycemia time in induction groups （（14.63±1.19） and （16.88±1.64） days） was significantly longer. The pathological examination showed less lymphocyte infiltration in induction groups. The IL-10 level and its mRNA in induction groups were significantly higher. Conclusions The HO-1 induced by CoPP would significantly improve function, prolong normoglycemia time and reduce lymphocyte infiltration. Meanwhile CoPP-induction in vivo had more beneficial effects than in vitro. Its mechanism could be related to immune-modulation of IL-10.

关键词： pancreatic islet transplantation cobalt protoporphyrin xenograft heme oxygenase interleukin 10

Prognostic factors of retroperitoneal soft tissue sarcomas:analysis of 132 cases

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Chinese Medical Journal 2007年第12期120卷 1047-1050页

作者： CHEN Chun-qiu YIN Lu PENG Cheng-hong CAI Yong li Ya-fen ZHAO Ren ZHOU Hui-jiang li hong-wei Department of General Surgery Ruijin Hospital Shanghai 200025 China Department of Epidemiology and Biostatistics Shanghai Jiao Tong University Shanghai 200025 China

Background Retroperitoneal sarcoma is a rare disease with poor prognosis. The aim of this study was to investigate the prognostic factors of the disease. Methods Between January 1988 and December 2003, 132 patients with retroperitoneal sarcoma were surgically treated in our hospital, 79 of them were followed up for 1-122 months （median： 19 months）. Their clinicopathological data including tumor size, histological subtype, grade and resection margin status, were studied. The Kaplan-Meier method and log-rank test were used to analyze the disease-specific survival rates after the resection. Results Among the 132 patients, 98 （74.2%） received macroscopic complete resection, 29 （22.0%） incomplete resection, and 5 （3.8%） surgical biopsy. In the 79 patients who were followed up, macroscopic clear resection of retroperitoneal sarcoma （n=49） was associated with a significantly higher survival rate compared with unclear resection （n=30, P〈 0.001）. The median survival period was 31 months （95%Cl, 20.09-41.91; actuarial 1-year survival, 85.7%） in the patients with the tumor completely resected and 11 months （95%Cl, 6.71-15.29; actuarial 1-year survival, 46.7%） in those with incomplete resection. Patients with high-grade sarcomas had a significantly shorter survival time （n=39; median： 24, 95%Cl： 5.71-42.29） than those with low-grade sarcomas （n=40; median： 15; 95%Cl： 8.80-21.20; P〈0.01）. Moreover, compared with the patients with the tumor sized 〈15 cm in diameter （n=53）, the survival rate was lower in those with a sarcoma sized 〉15 cm （n=26）. （Median： 12. 95%Cl： 8.26-15.74 vs median： 24, 95%Cl： 17.25-30.75; P〈 0.05）. Furthermore, the survival of the patients with liposarcomas （n=29, median： 29, 95%Cl： 12.84-45.16）, leiomyosarcomas （n=14, median： 11, 95%Cl： 6.11-15.89）, and others （n=-36, median： 22, 95%C/： 14.95-29.05） varied significantly （P〈0.05） Conclusion Completeness of resection, tumor volume, grade, and subtype are prognostic factors of retroperitoneal soft tissue

关键词： retroperitoneal neoplasm surgery prognosis

Tracking of CFSE-labeled endothelial progenitor cells in laser-injured mouse retina

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Chinese Medical Journal 2011年第5期124卷 751-757页

作者： SHI Hui YANG wei CUI Zhi-hua LU Cheng-wei li Xiao-hong liANG ling-ling SONG E Department of Ophthalmology First Hospital Jilin University Changchun Jilin 130021 China

Background Endothelial progenitor cells （EPCs） transplantation is a promising therapeutic strategy for ischemic retinopathy. The current study aimed to establish a simple, reliable and fluorescent labeling method for tracking EPCs with 5-（and-6）-carboxyfluorescein diacetate succinimidyl ester （CFSE） in laser-injured mouse retina. Methods EPCs were isolated from human umbilical cord blood mononuclear cells, cultivated, and labeled with various concentrations of CFSE. Based on fluorescence intensity and cell morphology, a 15 minutes incubation with 5 μmol/L CFSE at 37℃ was selected as the optimal labeling condition. The survival capability and the apoptosis rate of CFSE-labeled EPCs were measured by Trypan blue staining and Annexin V/PI staining assay respectively. Fluorescence microscopy was used to observe the label stability during the extended culture period. Labeled EPCs were transplanted into the vitreous cavity of pigmented mice injured by retinal laser photocoagulation. Evans Blue angiography and flat mounted retinas were examined to track the labeled *** EPCs labeled with 5 μmol/L CFSE presented an intense green fluorescence and maintained normal morphology, with no significant changes in the survival capability or apoptosis rate after being labeled for 2 days, 1 and 4 weeks, The fluorescence intensity gradually decreased in the cells at the end of 4 weeks. Evans Blue angiography of the retina displayed the retinal capillarity network clearly and fluorescence leakage was observed around photocoagulated spots in the laser-injured mouse model. One week after transplantation of labeled EPCs, the fluorescent cells were identified around the photocoagulated lesions. Four weeks after transplantation, fluorescent tube-like structures were observed in the retinal vascular *** EPCs could be labeled by CFSE in vitro and monitored in vivo for at least 4 weeks, and participate in the repair of injured retinal vessels.

关键词： endothelial progenitor cells 5-(and-6)-carboxyfluorescein diacetate succinimidyl ester cell tracking retina laser photocoagulation cell transplantation

中国塑性成形技术和装备30年的重大突破与进展

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塑性工程学报 2024年第4期31卷 2-45页

作者：林忠钦黄庆学苑世剑赵国群华林易幼平詹梅李淑慧刘伟王宝雨陈军王涛李宏伟黄亮何祝斌钟掘上海交通大学上海市复杂薄板结构数字化制造重点实验室上海200240 太原理工大学机械与运载工程学院山西太原030024 哈尔滨工业大学金属精密热加工国家级重点实验室黑龙江哈尔滨150001 山东大学材料科学与工程学院山东济南250061 武汉理工大学汽车工程学院湖北武汉430070 中南大学轻合金研究院湖南长沙410083 西北工业大学材料学院陕西西安710072 北京科技大学机械工程学院北京100083 上海交通大学材料科学与工程学院上海200240 华中科技大学材料科学与工程学院湖北武汉430074 大连理工大学机械工程学院辽宁大连116023

1994以来的30年,在国家重大需求的强劲牵引下,我国塑性成形技术与装备取得举世瞩目的巨大成就,研制出一大批世界第一的成形装备,实现了三大技术跨越,形成了规模最大的研究队伍,我国塑性成形技术总体水平进入世界先进国家行列,多个单项... 详细信息

1994以来的30年,在国家重大需求的强劲牵引下,我国塑性成形技术与装备取得举世瞩目的巨大成就,研制出一大批世界第一的成形装备,实现了三大技术跨越,形成了规模最大的研究队伍,我国塑性成形技术总体水平进入世界先进国家行列,多个单项技术和装备达到国际领先水平。选择了其中8项最具代表性成果,并介绍其在塑性工程理论和技术上取得的重大突破以及对国家重大装备研制的突出贡献,分析了我国塑性成形技术与国际领先水平的差距。最后对我国塑性成形技术发展将呈现出的“三超两高”五大发展趋势进行了展望。

关键词：金属成形技术装备塑性

Microstructure Evolution and Nanomechanical Behavior of Micro-Area in Molten Pool of Selective Laser Melting(CoCrNi)_(82)Al_(9)Ti_(9)High-Entropy Alloy

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Acta Metallurgica Sinica(English Letters) 2024年第6期37卷 1019-1033页

作者： hong-wei Zhang li-wei Lan Zhe-Yu Yang Chang-Chun li Wen-Xian Wang College of Materials Science and Engineering Taiyuan University of TechnologyTaiyuan030024China Ministry of Education Key Laboratory of Interface Science and Engineering in Advanced MaterialsTaiyuan University of TechnologyTaiyuan030024China Shanxi-Zhejiang University New Materials and Chemical Research Institute Taiyuan030032China

In this work,the phase evolution mechanism and nanomechanical properties of(CoCrNi))(82)Al_(9)Ti_(9)high-entropy alloy(HEA)prepared by selective laser melting(SLM)in the molten pool were *** HEA contains multiple primary elements and undergoes high-temperature gradient and rapid cooling during *** leads to significant inhomogeneity of nano-scale microstructure characteristics and instability of *** optimizing process parameters,the microstructure evolution at the optimal parameter volume energy density of 440 J/mm^(3)was studied.A phase transition from BCC to FCC occurred in the melt *** the micro-area of the melt pool results in a temperature rise and the combustion-induced loss of Al ***,the Ni element content increases significantly outside the melt *** process enhances the phase stability of FCC and facilitates phase ***,rapid cooling leads to the formation of distinctive ultrafine equiaxial crystals inside the melt pool,accompanied by the generation of intracrystalline needle-like nano-scale *** the melt pool,the accumulation of energy results in the formation of coarse ***,the nano-hardness inside the molten pool is remarkably high at 11.79 GPa,while the outside the molten pool is reduced to 9.58 *** the fracture toughness outside the melt pool also *** with inside the melt pool,the residual stress outside the melt pool changed from compressive to tensile stress and decreased from 603.28 to 322.84 MPa.

关键词： High-entropy alloys Selective laser melting Microstructure evolution Nanoindentation

Studies on Fragmentation Pathways of N-Ethoxy(phenyl) phosphoryl Amino Acids by Electrospray Ionization Tandem Mass Spectrometry

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Chemical Research in Chinese Universities 2006年第5期22卷 598-601页

作者： CAO Shu-xia li hong-wei GUO Yan-chun liAO Xin-cheng ZHAO Yu-fen The Key Laboratory of Chemical Biology and Organic Chemistry Department of Chemistry Zhengzhou University Zhengzhou 450052 P. R. China The Key Laboratory for Bioorganic Phosphorus Chemistry and Chemical Biology Ministry of Education Department of Chemistry School of Life Sciences and Engineering Tsinghua University Beijing 100084 P. R. China

The positive and negative ESI-MS/MS spectra of N-ethoxy（phenyl） phosphoryl amino acids（EPP-AA） were investigated by electrospray ionization（ESI） ion trap mass spectrometry. The fragmentation pathways of [ M ＋ Na]^＋ and [ M - H]^- ions are proposed and rationalized. The observation may have some potential applications in the interpretation of the MS/MS spectra of novel N-phosphoryl compounds. The complexity of MS/MS spectra of EPP-AA [ M ＋ Na]^＋ ions is decreased compared with that of N-dialkyloxyphosphoryl amino acid. Therefore, the new phosphonamidate method may be considered one of the superior methods that can be used in sequencing peptides and proteins extensively.

关键词： N-Ethoxy(phenyl) phosphoryl amino acid Electrospray ionization ion trap mass spectrometry Tandem mass spectrometry

Role of radial artery in total arterial myocardial revascularization in coronary bypass surgery

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Chinese Medical Journal 2008年第3期121卷 200-204页

作者： CHEN Xu-jun ZHANG Yong CHEN Xin FENG Wen-hui li Da-lian li hong-wei Department of Cardiac Surgery Cardiovascular Center BeijingFriendship Hospital Capital Medical University Beijing 100050China Department of Cardiac Surgery Beijing Military General HospitalBeijing 100700 China Department of Thoracic and Cardiovascular Surgery Nanjing FirstHospital Nanjing Medical University Nanjing Jiangsu 210006China

Background Total arterial revascularization （TAR） was widely utilized in coronary artery bypass grafting （CABG） as a result of its better long-term effect compared with vein grafts. Of the arterial conduits, radial artery （RA） gained popularity for its easy availability and reported long-term patency. Thus, the objective of this study was to investigate the effect of RA in TAR in CABG. Methods From January 2000 to December 2006, 85 patients （56 male and 29 female） at a mean age of 57.0 ± 5.2 years, underwent TAR in CABG. RA and left internal mammary artery （liMA） with composite Y or T and sequential grafting techniques were used. Post-operative complications were recorded and follow-up was performed. Results Eighty-five liMA and 149 RA grafts including 21 single and 64 bilateral RA were collected. A total of 87 distal anastomoses were done with the liMA and another 152 were done with the RA, with the mean number of distal anastomosis per patient of 2.81 ± 0.47. The proximal RA ends were anastomsed directly to the aorta in 140 grafts with Y or T graft off in situ liMA in 9, Y or T graft off RA in 9. The distal end was anastomsed to right coronary artery system in 92 to obtuse margina in 46, to diagonal in 19 and to ramous intermedius in 5. Nine sequential anastomoses were performed with RA. Nine composite Y or T grafts were constructed with RA and liMA while another 9 were constructed with RA and RA. One （1.2%） patient died, 3 patients （3.5%） experienced acute renal failure and 2 （2.4%） developed stroke. All patients were still alive and no patient had evidences of newly occurred myocardial infarction or angina after a mean follow-up of 36.5 ＋ 4.1 months （6-67 months）. Postoperatively at 6 month, mean left ventricular ejective fraction was increased to 0.49 ±0.09, compared with that of 0.43 ± 0.11 preoperatively （P=-0.027）. Postoperative mean New York Heart Association class was 2.5±0.5, compared with that of 3.0±0.4 preoperatively （P=-0.003）. Conclusions TAR w

关键词： radial artery coronary artery bypass total arterial revascularization

后内侧壁骨折块复位固定对PFNA治疗股骨粗隆间骨折合并后内侧壁骨折的临床效果

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哈尔滨医科大学学报 2024年第2期58卷 188-192页

作者：张浩张斌章其祥李洪伟徐州医科大学第一临床学院骨科江苏徐州221004 沭阳县中医院骨科江苏宿迁223600 徐州医科大学附属医院骨科江苏徐州221002

目的探讨后内侧壁骨折块复位固定对股骨近端防旋髓内钉(proximal femoral nail antirotation,PFNA)治疗股骨粗隆间骨折合并后内侧壁骨折的临床效果。方法回顾性分析2020年1月~2022年1月沭阳县中医院收治的64例股骨粗隆间骨折合并后内侧... 详细信息

目的探讨后内侧壁骨折块复位固定对股骨近端防旋髓内钉(proximal femoral nail antirotation,PFNA)治疗股骨粗隆间骨折合并后内侧壁骨折的临床效果。方法回顾性分析2020年1月~2022年1月沭阳县中医院收治的64例股骨粗隆间骨折合并后内侧壁骨折患者的资料。所有患者均行PFNA治疗,根据是否采用后内侧壁骨折块复位固定分为未复位组(n=29例)和复位组(n=35例)。比较2组患者手术时间、术中出血量、负重时间、骨折愈合时间,髋关节活动度,视觉模拟疼痛评分(visual analogue scale,VAS)、髋关节功能评分、下肢功能评分以及并发症发生情况。结果复位组患者手术时间长于未复位组(P0.05)。复位组患者负重时间、骨折愈合时间均短于未复位组(P0.05)。结论后内侧壁骨折块复位固定用于PFNA治疗股骨粗隆间骨折合并后内侧壁骨折患者,可以加快术后恢复,减轻术后疼痛,改善髋关节和下肢功能,安全可靠。

关键词：后内侧壁骨折骨折复位股骨近端防旋髓内钉股骨粗隆间骨折