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Photoregulated or Energy Dependent Process of Hormogonia Differentiation in Nostoc sphaeroides Kutzing （Cyanobacterium）

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Journal of Integrative Plant Biology 2005年第6期47卷 709-716页

作者： Dun-Halli Lan-ZhouCHEN Gen-Baoli Gao-HongWANG li-rongsong Yong-DingliU StateKeyLaboratoryofFreshwaterEcologyandBiotechnology InstituteofHydrobiologytheChineseAcademyofSciencesWuhan430072China SchoolofResourceandEnvironmentSciences WuhanUniversityWuhan430072China

Hormogonium, which was thought to play an important role in the dispersal and survival of these microorganisms in their natural habitats, is a distinguishable developmental stage of heterocystous cyanobacteria. The present study examined the effects of different light conditions and sugars on the differentiation ofNostoc sphaeroides Kiitzing to the hormogonia stage. Results showed that differentiation of hormogonia was light dependent in the absence of sugar, but that close to 100% of cyanobacteria differentiated to hormogonia in the presence of glucose or sucrose, irrespective of the light conditions. This differentiation was inhibited, even in the presence of sugars, upon application of an inhibitor of respiration. Following the testing of different sugars, the effects of different lights were examined. It was found that 5-10μmol·m^-2·s^-1 photon flux density was optimal for hormogonia differentiation. One hundred percent differentiation was obtained with white light irradiation, in contrast with irradiation with green light (80% differentiation) and red light (0-10% differentiation). Although they showed different efficiencies in inducing hormogonia differentiation in N. sphaeroides, the green and red radiation did not display antagonistic effects. When the additional aspect of time dependence was investigated through the application of different fight radiations and an inhibitor of protein synthesis, it was found that the initial 6 h of the differentiation process was crucial for hormogonia differentiation. Taken together, these results show that hormogonia differentiation in N. sphaeroides is either a photoregulated or an energy deoendent orocess.

关键词： cyanobacterium energy-dependent hormogonia differentiation Nostoc sphaeroides Kützing photoregulation.

RNPS1 stabilizes NAT10 protein to facilitate translation in cancer via tRNA ac^(4)C modification

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International Journal of Oral Science 2024年第1期16卷 73-84页

作者： Xiaochen Wang rongsong ling Yurong Peng Weiqiong Qiu Demeng Chen Center For Translational Medicine the First Affiliated HospitalSun Yat-sen UniversityGuangzhouChina Institute for Advanced Study Shenzhen UniversityShenzhenChina

Existing studies have underscored the pivotal role of N-acetyltransferase 10(NAT10) in various cancers. However, the outcomes of protein-protein interactions between NAT10 and its protein partners in head and neck squamous cell carcinoma(HNSCC) remain unexplored. In this study, we identified a significant upregulation of RNA-binding protein with serine-rich domain 1(RNPS1) in HNSCC, where RNPS1 inhibits the ubiquitination degradation of NAT10 by E3 ubiquitin ligase, zinc finger SWIM domain-containing protein 6(ZSWIM6), through direct protein interaction, thereby promoting high NAT10 expression in HNSCC. This upregulated NAT10 stability mediates the enhancement of specific tRNA ac^(4)C modifications, subsequently boosting the translation process of genes involved in pathways such as IL-6 signaling, IL-8 signaling, and PTEN signaling that play roles in regulating HNSCC malignant progression, ultimately influencing the survival and prognosis of HNSCC patients. Additionally, we pioneered the development of TRMC-seq, leading to the discovery of novel t RNA-ac^(4)C modification sites, thereby providing a potent sequencing tool for tRNAac^(4)C research. Our findings expand the repertoire of tRNA ac^(4)C modifications and identify a role of tRNA ac^(4)C in the regulation of mRNA translation in HNSCC.

关键词： NAT1 thereby translation

Single cell analysis unveils B cell-dominated immune subtypes in HNSCC for enhanced prognostic and therapeutic stratification

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International Journal of Oral Science 2024年第3期16卷 448-459页

作者： Kang li Caihua Zhang Ruoxing Zhou Maosheng Cheng rongsong ling Gan Xiong Jieyi Ma Yan Zhu Shuang Chen Jie Chen Demeng Chen liang Peng State Key Laboratory of Oncology in South China Department of Oral and Maxillofacial SurgeryInstitute of Precision MedicineCenter for Translational MedicineThe First Affiliated Hospital of Sun Yat-sen UniversityGuangzhouChina Institute for Advanced Study Shenzhen UniversityShenzhenChina Hospital of Stomatology Guangdong Provincial Key Laboratory of StomatologyGuanghua School of StomatologySun Yat-Sen UniversityGuangzhouChina Senior Department of Oncology The Fifth Medical Center of PLA General HospitalBeijingChina

Head and neck squamous cell carcinoma(HNSCC)is characterized by high recurrence or distant metastases rate and the prognosis is *** is mounting evidence that tumor-infiltrating B cells(TIL-Bs)have a crucial,synergistic role in tumor ***,little is known about the role TIL-Bs play in immune microenvironment and the way TIL-Bs affect the outcome of immune checkpoint *** single-cell RNA sequencing(scRNA-seq)data from the Gene Expression Omnibus(GEO)database,the study identified distinct gene expression patterns in *** samples were categorized into TIL-Bs inhibition and TIL-Bs activation groups using unsupervised *** classification was further validated with TCGA HNSCC data,correlating with patient prognosis,immune cell infiltration,and response to *** found that the B cells activation group exhibited a better prognosis,higher immune cell infiltration,and distinct immune checkpoint levels,including elevated PD-L1.A prognostic model was also developed and validated,highlighting four genes as potential biomarkers for predicting survival outcomes in HNSCC ***,this study provides a foundational approach for B cells-based tumor classification in HNSCC,offering insights into targeted treatment and immunotherapy strategies.

关键词： prognosis elevated offering

英诺克李斯特菌侵染果蝇S2细胞后的转录组分析

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华南农业大学学报 2020年第5期41卷 17-26页

作者：吴坤钟李荣松曹阳黄志君田铃华南农业大学动物科学学院/广东省农业动物功能基因组学与分子育种重点实验室/广东省蚕桑工程技术研究中心广东广州510642

【目的】英诺克李斯特菌listeria innocua为李斯特菌属listeria的一种非致病菌,与致病菌单增李斯特菌***具有共同的毒力祖先,本文旨在研究英诺克李斯特菌侵染后宿主基因表达的变化情况,为宿主调控、防御单增李斯特菌的危害提供参考。【... 详细信息

【目的】英诺克李斯特菌listeria innocua为李斯特菌属listeria的一种非致病菌,与致病菌单增李斯特菌***具有共同的毒力祖先,本文旨在研究英诺克李斯特菌侵染后宿主基因表达的变化情况,为宿主调控、防御单增李斯特菌的危害提供参考。【方法】用英诺克李斯特菌侵染果蝇S2细胞,利用转录组测序检测该细菌侵染后果蝇S2细胞的基因表达变化,采用qPCR验证英诺克李斯特菌侵染后果蝇S2细胞中差异表达的基因。【结果】英诺克李斯特侵染3 h后,宿主果蝇S2细胞中Toll/Imd信号通路发生显著上调,吞噬体和霍乱弧菌Vibrio cholerae感染信号通路显著下调;抗菌肽基因DmDef(DmDefensin)、DmDro(DmDrosomycin)、DmDpt A(DmDptericin A)、DmDpt B(DmDptericinB)、 DmMtk(DmMetchnikowin)、 DmCec A2(DmCecropinA2)、 DmAtt A(DmAttacinA)、 DmAtt B(DmAttacin B)、DmAtt D(DmAttacin D)、DmCec B(DmCecropin B)均被显著地诱导表达,其中,上调幅度最大的基因是DmDef,上调了9.805倍。qPCR验证结果显示,DmMtk、DmAtt A、DmDro和DmDef基因表达分别上调了8.180、7.533、7.204和4.569倍。【结论】英诺克李斯特菌侵染果蝇S2细胞后,变化最显著的基因类群为抗菌肽。本研究全面调查了英诺克李斯特菌侵染后宿主基因表达的变化情况,为揭示非致病菌侵染后宿主细胞的反应、细菌与宿主的互作规律提供了参考。

关键词：英诺克李斯特菌果蝇S2细胞转录组测序免疫抗菌肽信号通路

Single-cell transcriptomics reveals cell atlas and identifies cycling tumor cells responsible for recurrence in ameloblastoma

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International Journal of Oral Science 2024年第2期16卷 251-264页

作者： Gan Xiong Nan Xie Min Nie rongsong ling Bokai Yun Jiaxiang Xie linlin Ren Yaqi Huang Wenjin Wang Chen Yi Ming Zhang Xiuyun Xu Caihua Zhang Bin Zou Leitao Zhang Xiqiang liu Hongzhang Huang Demeng Chen Wei Cao Cheng Wang Hospital of Stomatology Sun Yat-sen UniversityGuangzhouChina Guangdong Provincial Key Laboratory of Stomatology Sun Yat-sen UniversityGuangzhouChina Guanghua School of Stomatology Sun Yat-sen UniversityGuangzhouChina Department of Periodontics Affiliated Stomatology Hospital of Guangzhou Medical UniversityGuangzhou Key Laboratory of Basic and Applied Research of Oral Regenerative MedicineGuangzhouChina Institute for Advanced Study Shenzhen UniversityShenzhenChina Center for Translational Medicine The First Affiliated HospitalSun Yat-sen UniversityGuangzhouChina State Key Laboratory of Ophthalmology Zhongshan Ophthalmic CenterSun Yat-sen UniversityGuangzhouChina Department of Oral and Maxillofacial Surgery Nanfang HospitalSouthern Medical UniversityGuangzhouChina Department of Oral and Maxillofacial&Head and Neck Oncology Shanghai Ninth People’s HospitalShanghai Jiao Tong University School of MedicineShanghaiChina National Center for Stomatology National Clinical Research Center for Oral diseasesShanghai Key Laboratory of StomatologyShanghaiChina

Ameloblastoma is a benign tumor characterized by locally invasive phenotypes,leading to facial bone destruction and a high recurrence ***,the mechanisms governing tumor initiation and recurrence are poorly ***,we uncovered cellular landscapes and mechanisms that underlie tumor recurrence in ameloblastoma at single-cell *** results revealed that ameloblastoma exhibits five tumor subpopulations varying with respect to immune response(IR),bone remodeling(BR),tooth development(TD),epithelial development(ED),and cell cycle(CC)*** note,we found that CC ameloblastoma cells were endowed with stemness and contributed to tumor recurrence,which was dominated by the EZH2-mediated *** EZH2 effectively eliminated CC ameloblastoma cells and inhibited tumor growth in ameloblastoma patient-derived *** data described the tumor subpopulation and clarified the identity,function,and regulatory mechanism of CC ameloblastoma cells,providing a potential therapeutic target for ameloblastoma.

关键词： inhibited cycling eliminated

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路灯专用配电变压器联接组的选择

现代建筑电气 2018年第1期9卷 1-4页

作者：程澍李荣松胡军浙江省建筑设计研究院浙江杭州310006 杭州萧山国际机场有限公司浙江杭州311207

比较了配电变压器常用联接组别,以某大型机场内路灯专用变压器改造为契机,分析了适合路灯专用的变压器联接组的选择。提出要根据实际工程项目具体情况对路灯负荷进行分析,再选择合适的变压器联接组别,对日后的运营维护带来极大便利。

关键词：路灯变压器联接绕组联接形式防雷能力

Semi-automated detection of rangeland runoff and erosion control berms using high-resolution topography data

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International Soil and Water Conservation Research 2024年第1期12卷 217-226页

作者： li li Changjiang River Scientific Research Institute of Changjiang Water Resources Commission Wuhan430010China Research Center on Mountain Torrent&Geologic Disaster Prevention Ministry of Water ResourcesWuhan430010China University of Arizona School of Natural Resources and the EnvironmentTucsonAZ85719USA

An inventory of topographic modifications is essential to addressing their impacts on hydrological and morphological processes in human-altered ***,such inventories are generally *** study presents two workflows for semi-automatic detection of linear earthen runoff and erosion control berms in rangelands using high-resolution topographic *** workflows consist of initial object identification by applying either morphological grayscale reconstruction(MGR)or the Geomorphon(GEO)method,followed by identification refinements through filters based on objects’horizontal and vertical *** sites were selected within the Altar Valley,Arizona,in the southwestern United *** site was used for developing workflows and optimizing filter thresholds,and the other two sites were used to validate *** results showed that:1)The MGR-based workflow methodology could produce final precision and detection rates of up to 92%and 75%,respectively,and take less than 5 s for a 10.1 km^(2) site;2)The workflow based on the MGR method yielded greater identification accuracy than did the GEO workflow;3)Object length,orientation,and eccentricity were important characteristics for identifying earthen berms,and are sensitive to general channel flow direction and berm shape;4)Manual interrogation of topographic data and imagery can significantly improve identification precision *** proposed workflows will be useful for developing inventories of runoff and erosion control structures in support of sustainable rangeland management.

关键词： Earthen berm Rangeland Grayscale morphological reconstruction Geomorphon liDAR

Failure mechanisms and surface treatment processes of thermal barrier coatings:Review

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Chinese Journal of Aeronautics 2024年第9期37卷 20-40页

作者： Hao li Shengqiang YANG Xiuhong li Wenhui li College of Mechanical and Vehicle Engineering Taiyuan University of TechnologyTaiyuan 030024China Shan.xi Province Key Laboratory of Precise Machining Taiyuan University of TechnologyTaiyuan 030024China College of Aeronautics and Astronautics Taiyuan University of TechnologyTaiyuan 030024China

Thermal Barrier Coatings(TBCs)technology is key to improving the service temperature and the productivity of aircraft *** performance and failure life of TBCs are strongly influenced by surface integrity and ***,recognizing failure mechanisms and developing effective surface treatment processes are crucial for further improving the reliability and durability of *** paper explains the primary reasons for TBC failure,emphasizing on how integrity of surface and interface influences interfacial oxidation,high-temperature erosion,and Calcium-Magnesium-Alumina-Silicate(CMAS)***,this paper completely and rigorously evaluates the research status of TBCs surface treatment processes,including the characteristics and effects of various processes,and describes the requirements and goals of pretreatment and *** addition,a potential direction for the development and application of TBCs surface treatment is suggested.

关键词： Thermal BarrierCoatings(TBCs) Failure Surface treatment processes Processing goals Mass finishing

Effects of mid-passage gap with a variable surface angle on a turbine vane endwall’s aerothermal and film cooling performances

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Chinese Journal of Aeronautics 2024年第6期37卷 20-37页

作者： Zhiyu li Kaiyuan ZHANG Zhigang li Jun li Institute of Turbomachinery Xi’an Jiaotong UniversityXi’an 710049China

The mid-passage gap is an inevitable structure in a vane passage due to turbine vanes being manufactured *** coolant from this gap is able to prevent hot mainstream ingression and provide cooling protection for the endwall.A novel idea of enlarging the endwall’s coverage area and reducing the endwall’s thermal load by applying the mid-passage gap with variable surface angles is carried out in this *** endwall’s aerothermal and film cooling performances under four mid-passage gap modes at three typical mass flow ratio conditions are numerically *** indicate that under the traditional mid-passage mode,the coolant flows into the mainstream with a perpendicular incidence angle and can’t stick to the ***,cooling failure occurs,and the endwall’s thermal load is badly *** film cooling level at the suction-side endwall is improved when applying the mid-passage gap of a 45surface angle due to the secondary vortex being *** addition,when applying the mid-passage gap of a 135surface angle,the horseshoe vortex is pushed away,and the coverage area at the pressure-side endwall is enlarged *** best film cooling performance is achieved when the upstream surface angle is 135and the downstream surface angle is 45due to the adiabatic film cooling effectiveness being increased at both the pressure-and suction-side *** the mass flow ratio is 1.5%,the coverage area is enlarged by 43%,and the area-averaged adiabatic film cooling effectiveness is increased by 37%,when compared with those under the traditional mid-passage mode.

关键词： Film cooling Heat transfer Surface angle Mid-passagegap Turbinevane endwall