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The surge-heave-pitch coupling motions of the Φ- type vertical axis wind turbine supported by the truss Spar floating foundation

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Journal of Hydrodynamics 2019年第4期31卷 669-681页

作者： Ying Guo li-qin liu Yan li Chang-shui Xiao You-gang Tang State Key Laboratory of Hydraulic Engineering Simulation and Safety Tianjin University Tianjin 300072 China

The motions of the floating vertical axis wind turbine (VAWT) with consideration of the coupling between the aerodynamics and the hydrodynamics are calculated in this paper. The surge-heave-pitch coupling nonlinear equations of the floating VAWT are established. The aerodynamic loads are obtained by applying the double-multiple-stream tube theory with consideration of the dynamic stall and the floating foundation motion. The motion performances of a 5 MW Φ - Darrieus type floating VAWT are studied with its foundation of the Spar type with heave plates. It is shown that the amplitude of the heave motion of the floating VAWT is small when the heave plates are equipped. The effects of the wind and wave loads on the floating VAWT motions are assessed. The results show that the mean values of the surge and the pitch of the floating VAWT are mainly related to the wind loads;the standard deviations of the surge, the heave, and the pitch are mainly related to the wave loads. In the regular wave cases, the frequencies of the surge, the heave and the pitch are dominated by the wave frequencies, and the components of the 2P responses (caused by the aerodynamic loads) of the pitch are small. The 2P responses of the pitch are more significant in the irregular wave cases as compared with those in the regular wave cases.

关键词： Floating VAWT truss Spar floating foundation surge-heave-pitch motions aerodynamics regular waves irregular waves

Hydrated nickel(Ⅱ) halides mediated ring opening reaction with N-tosylaziridines

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Chinese Chemical Letters 2012年第3期23卷 285-288页

作者： Wan Xuan Zhang Wei Gang Hu li Su li qin liu Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules Hubei UniversityWuhan 430062China

An efficient and water tolerant method for the synthesis ofβ-haloamines is described utilizing hydrated nickel（Ⅱ） halides （NiX_2ⅩnH_2O X = Cl,Br,I） and aziridines as starting materials.N-Tosylaziridines reacted with NiCl_2·6H_2O or NiI_2·6H_2O givingβ-chloro -orβ-iodoamines in high yields（73-99%） within a short time,but 10 mol%of n-Bu_4NBr should be added in the reactions of N-tosylaziridines with NiBr_2·3H_2O in order to obtain the high yields of correspondingβ-*** played an important role in the *** proper solvent for the reaction of NiCl_2·6H_2O was DMF,while NiBr_2·3H_2O or NiI_2·6H_2O proceeded well in 1,4-dioxane.

关键词： N-Tosylaziridines Hydrated nickel(Ⅱ) halides Ring opening reaction β-Haloamines

Analysis of risk factors for postoperative deep vein thrombosis after craniotomy and nomogram model construction

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World Journal of Clinical Cases 2023年第31期11卷 7543-7552页

作者： Zhen-Jin Su Hong-Rui Wang li-qin liu Nan li Xin-Yu Hong Department of Neurosurgery The First Hospital of Jilin UniversityChangchun 130000Jilin ProvinceChina Department of Operating Theater The First Hospital of Jilin UniversityChangchun 130000Jilin ProvinceChina

BACKGROUND Deep vein thrombosis(DVT)of the lower extremity is one of the most common postoperative complications,especially after craniocerebral *** may lead to pulmonary embolism,which has a devastating impact on patient *** study aimed to investigate the incidence and risk factors of DVT in the lower limbs following craniocerebral *** To identify independent risk factors for the development of postoperative DVT and to develop an effective risk prediction *** The demographic and clinical data of 283 patients who underwent craniocerebral surgery between December 2021 and December 2022 were retrospectively *** independent risk factors for lower extremity DVT were identified by univariate and multivariate analyses.A nomogram was created to predict the likelihood of lower extremity DVT in patients who had undergone craniocerebral *** efficacy of the prediction model was determined by receiver operating characteristic curve using the probability of lower extremity DVT for each *** Among all patients included in the analysis,47.7%developed lower extremity DVT following craniocerebral *** risk of postoperative DVT was higher in those with a longer operative time,and patients with intraoperative intermittent pneumatic compression were less likely to develop postoperative *** The incidence of lower extremity DVT following craniocerebral surgery is significant,highlighting the importance of identifying independent risk *** such as the use of intermittent pneumatic compression during surgery may prevent the formation of postoperative DVT.

关键词： Deep vein thrombosis Craniotomy surgery Risk factors Nomogram

Quantification of Simian Immunodeficiency Virus by SYBR Green RT-PCR Technique

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Virologica Sinica 2008年第3期23卷 189-195页

作者： Jing LU li qin Guang-jie liu Si-ting ZHAO Xiao-ping CHEN Guangzhou Institute of Biomedicine and Health （GIBH） Chinese Academy of Sciences Guangzhou 510663China

Plasma viral RNA load is widely accepted as the most relevant parameter to assess the status and progression of Simian immunodeficiency virus （SIV） infections. To accurately measure RNA levels of the virus, a one-step fluorescent quantitative assay was established based on the SYBR green Real-time reverse transcription-polymerase chain reaction （RT-PCR）. The lower detection limit of the assay was 10 copies per reaction for the virus. This method was successfully applied to quantify SIVmac251 and SIVmac239 viruses produced in CEMx174 cells. Additionally, the performance of the SYBR green RT-PCR was assessed in a SIVmac251 infected rhesus macaque. The result demonstrated that the method could detect as little as 215 copies per milliliter of plasma and the dynamic pattern of viral load was highly consistent with previous results. With regard to convenience, sensitivity and accuracy our assay represents a realistic alternative to both branched-chain DNA （b-DNA） assays or real-time PCR assays based on TaqMan probes.

关键词： Real time PCR (RT-PCR) SYBR green Simian immunodeficiency virus (SIV) Viral loads

Chromosome-level genome assembly of the dotted gizzard shad(Konosirus punctatus) provides insights into its adaptive evolution

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Zoological Research 2022年第2期43卷 217-220页

作者： Bing-Jian liu Kun Zhang Shu-Fei Zhang Yi-Fan liu Jia-Sheng li Ying Peng Xun Jin Yun-Peng Wang Si-Xu Zheng li Gong li-qin liu Zhen-Ming Lu National Engineering Laboratory of Marine Germplasm Resources Exploration and Utilization Zhejiang Ocean UniversityZhoushanZhejiang 316022China Guangdong Provincial Key Laboratory of Fishery Ecology and Environment South China Sea Fisheries Research InstituteChinese Academy of Fisheries SciencesGuangzhouGuangdong 510300China National Engineering Research Center for Facilitated Marine Aquaculture Marine Science and Technology CollegeZhejiang Ocean UniversityZhoushanZhejiang 316022China

Konosirus punctatus is an economically important marine fishery resource and is widely distributed from the Indian to Pacific *** is a good non-model species for genetic studies on salinity and temperature ***,a high-... 详细信息

关键词： salinity Indian evolution

Study of the natural vibration characteristics of water motion in the moon pool by the semi-analytical method

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Journal of Hydrodynamics 2017年第1期29卷 124-134页

作者：刘利琴李妍黄磊唐友刚 State Key Laboratory of Hydraulic Engineering Simulation and Safety Tianjin UniversityTianjin 300072China

The three-dimensional natural vibration characteristics of water inside a moon pool of an ocean structures are studied. The governing equations are derived based on the linear potential flow theory, and the boundary condition of the total opening bottom suggested by Molin is adopted. A semi-analytical method is used to solve the governing equations, and the natural frequencies and the motion modes are obtained. Two types of motions are studied：（1） the piston motion in the vertical direction, and （2） the sloshing motion of the free surface. The influences of moon pool＇s structural parameters on the natural frequencies, and the modal shapes are analyzed.

关键词： Moon pool natural vibration characteristics semi-analytical method parameter influences

Gene and protein expressions of p28^(GANK)in rat with liver regeneration

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World Journal of Gastroenterology 2003年第11期9卷 2523-2527页

作者： Jian-Min qin Xiao-Yong Fu Shen-Jing li Shu-qin liu Jin-Zhang Zeng Xiu-Hua Qiu Meng-Chao Wu Hong-Yang Wang, International Co-operation Laboratory on Signal Transduction, Eastern Hepatobiliary Surgery Institute, Second Military Medical University, Shanghai, 200438, China International Co-operation Laboratory on Signal Transduction Eastern Hepatobiliary Surgery Institute Second Military Medical University Shanghai 200438 China.

AIM: To observe the gene and protein expression changes of p28GANK in regenerating liver tissues, and to reveal the biological function of p28GANK on the regulation of liver ***: One hundred and thirty two adult male Sprague-Dawley rats were selected, weighing 200-250 g,and divided randomly into sham operation (SO) group and partial hepatectomy (PH) group. Each group had eleven time points: 0, 2, 6, 12, 24, 30, 48, 72, 120, 168 and 240 h,six rats were in each time point. The rats were undergone 70 % PH under methoxyflurane anesthesia by resection of the anterior and left lateral lobes of the liver. SO was conducted by laparotomy plus slight mobilization of the liver without resection. liver specimens were collected at the indicated time points after PH or SO. The expression level of p28GANK mRNA was determined by Northern blot as well as at protein level via immunohistochemical *** expressions of p28GANK mRNA in these tissues were analyzed by imaging analysis system of FLA-2000 FUJIFILM and one way analysis of variance. The protein expressions of p28GANK in these tissues were analyzed with Fromowitz'method and Rank sum ***: The expression of p28GANK mRNA in bhe regenerating liver tissues possessed two transcripts, which were 1.5 kb and 1.0 kb. There was a significantly different expression patterns of p28GANK mRNA between SO and PH groups (Pliver regeneration, the pr

关键词： p28GANK 肝组织重建基因表达动物实验细胞增殖作用机制

Association of Chronic Kidney Disease with Non-alcoholic Fatty liver Disease in Community Population with Increased Fasting Blood-glucose Level

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器官移植内科学杂志 2014年第2期9卷 54-59页

作者： Yongqiang li Shuangshuang Zhu Xiaofei Shao Xinyu liu Aiqun liu Ying Zhang Honglei Wang Bin li Kangping Deng qin liu Harry Holthofer Hequn Zou Institute of Nephrology and Urology The Third Affiliated Hospital of Southern Medical University Guangzhou 510630 China. National Centre for Sensor Research/BioAnalytical Sciences Dublin City University Dublin 9 Ireland.

Comparison between the Effects of Intraperitoneal Injection of LDL and Intravenous Injection of LDL on Arterial Endothelial Cells Apoptosis

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Journal of Huazhong University of Science and Technology(Medical Sciences) 2003年第2期23卷 121-123页

作者：王丽秦瑾刘正湘 Department of Cardiology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430030

To observe the effect of oxidized low density lipoprotein (OxLDL) on arterial endothelial cells apoptosis in vivo, we established a model in which Sprague-Dawley rats were given intraperi-toneal and intravenous injection of unmodified LDL (8 mg/kg every day) via the tail vein. Seven days after the injection, the aortic endothelial cells specimens were prepared by an en face preparation of rat aorta. The apoptotic cells were identified and counted by in situ nick and labelling (TUNED method and light microscopy. The numbers of the apoptotic cells were 12. 52±4. 71/field in the in-traperitoneal injection control group, 11. 41±2. 94/field in the intravenous injection control group, 22. 98±8. 01/field in the intraperitoneal injection LDL group and 103. 8±11. 5/field in the intravenous injection LDL group, respectively. The difference was significant between injection LDL group and control (Plial cells and the effect is especially significant with intravenous injection LDL. After injection, oxidative modification of LDL may occur in local arteries and causes injury to the endothelial cells.

关键词： arterial endothelial cells apoptosis LDL