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The comparisons on total RNA from different source-original neurons applied in LMPC

Neuroscience Bulletin 2006年第2期22卷 91-96页

作者： Jun LEI JP Dai li-qiang ru Guang-Fu YIN CG Van Eden RM Buijs Huazhong University of Science and Technology Wuhan 430030China Netherlands Institute for Brain Research Meibergdreef 33 1105 AZ Amsterdam

Objective To compare the quality and quantity of total RNA from different source-original neurons applied in LMPC technique. Methods （ 1 ） Aglient 2100 bioanalyzer and RT-PCR were used to check the concentration and fragmentation of total RNA from unfixed, temporal fixed and fixed 12 h hypothalamus sections; （2）Different neurons of PVN and SON were collected by LMPC, CRH, TRH, AVP, OT mRNA level were measured by RT-PCR; （3）Labeled neurons by injecting CTB into stomach and non-labeled neurons in DMV collected by LMPC were checked for house keeping genes by RT-PCR. Results （ 1 ） Unfixed section had higher concentration and better quality of total RNA compared with fixed sections applied in LMPC ; relative short amplicons such as GAPDH, NSE, MCH and MCAR were successfully obtained from fixed and unfixed and long amplicon of GR can only be obtained from unfixed material; （2） In magnocellular PVN and SON the expressions of AVP and OT were more special than those in the parvocellular PVN. Oppositely, the expressions of CRH, TRH in the parvocellular were more special than the other two ; （3） The expressions of house keeping genes had no significant difference between labeled and non-labeled DMV neurons. Conclusion The quality and quantity of total RNA from unfixed brain tissues were better than fixed tissues applied in LMPC and the CTB tracer which may differentiate neurons had no significant effect on physiology of the neurons applied in LMPC. The results showed that the LMPC technique is suitable for the qualitative and quantitative study on individual neurons at mRNA level.

关键词： laser microdissection and pressure catapulting (LMPC) RT-PCR fixation PVN SON DMV

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Evaluation of Diagnostic Efficiency of Ultrasound Features on Malignant Thyroid Nodules in Chinese Patients

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Chinese Medical Journal 2016年第15期129卷 1784-1788页

作者： ru-qiang li Ge-Heng Yuan Ming Chen Yi-Min Shao Sai-Nan Zhu Jun-Qing Zhang Xiao-Hui Guo Department of Endocrinology Peking University First Hospital Beijing 100034 China Department of Endocrinology Tongzhou Xinhua Hospital Beijing 101100 China Department of UItrasonography Peking University First Hospital Beijing 100034 China Department of Statistics Peking University First Hospital Beijing 100034 China

Background： The aim of this study was to evaluate the efficacy of ultrasonic features in predicting the malignancy of thyroid nodules in a group of Chinese patients. Methods： In all, 762 patients with thyroid nodules （424 malignant and 338 benign） underwent ultrasound （US） check and surgery between March 2011 and July 2014 at Peking University First Hospital were identified. Univariate and receiver operating characteristic （ROC） analyses were performed to calculate the sensitivity, specificity, negative and positive predictive values of each US feature, and the accuracy of their combinations lbr prediction of malignancy. Results： Patients with malignant nodules were younger and without obvious risk history than those in the benign group （P 〈 0.001, P = 0.93 ）. No individual US sign was fully predictive of a malignant lesion. The Youden indexes of irregular margins and hypoechogenicity were the first and second highest in all US features, which were 51.9% and 45.2%, respectively. The sensitivity of solid components （89.7%） and hypoechogenicity （89.2%） and the specificity of taller-than-wide shape （98.5%） and microcalcifications （90.6%） were the first and second highest in all US features. Intranodular flow on a color Doppler examination was a weak predictor of malignancy. Under ROC analysis excepting intranodular flow, the 95% confidence interval （C1） of areas under tile curves of hypoechogenicity and irregular margins with any one of the US features were overlapped that of five-feature combinations （95% CI： 0.850 0.901）. Conclusions： We should be alert with taller-than-wide shape and microcalcifications, lntranodular flow was a weak predictor of malignancy. According to Youden indexes and ROC analysis, irregular margins and hypoechogenicity combined with solid component or taller-than-wide shapes or microcalcifications have a high predicative value for malignant thyroid nodules in Chinese patients.

关键词： Malignancy Thyroid Nodules Ultrasonography

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Effects of tanshinone on neuropathological changes induced by amyloid β-peptide(1-40)injection in rat hippocampus

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Acta Pharmacologica Sinica 2004年第7期25卷 15-22页

作者： Long-xuan li2, Jia-pei DAI3, li-qiang ru4, Guang-fu YIN4, Bin ZHAO Department of Neurology, Affiliated Hospital, Guangdong Medical College, Zhanjiang 524001, China 3The Netherlands Institute for Brain Research, Amsterdam 1105AZ, the Netherlands 4Department of Neurobiology, TongjiMedical College of Huazhong Universityof Science and Technology, Wuhan 430030, China Department of Neurology Affiliated Hospital Guangdong Medical College Zhanjiang 524001 China. Longxuanlee30@ hotmail.co

AIM: To investigate the effect of tanshinone (Tan) on the neuropathological changes induced by amyloid β- peptide1-40(Aβ1-40) injection in hippocampus in rats. METHODS: Aβ1-4010 μg was injected bilaterally into the dorsal blade of the dentate gyrus in the hippocampus. The level of acetylcholinesterase (AChE) in hippocampus was evaluated by histochemistry. The expressions of neuronal nitric oxide synthase (nNOS) and inducible form of NOS (iNOS) were detected by immunohistochemistry and Western blot. Aβ1-40-injected rats were treated ig with Tan, the major active ingredient from Salvia miltiorrhiza of Chinese herb extract. RESULTS: The level of AChE positive fibers of each subfield in Aβ1-40-injected hippocampus decreased significantly compared with those of control (Prus (DG)subfields (P<0.01). CONCLUSION: Tan can protect the neuropathological changes induced by Aβ1-40 injection in hippocampus.

关键词： tanshinone amyloid beta-protein acetylcholinesterase nitric oxide synthase hippocampus

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基于多尺度奇异谱分析的转子碰磨诊断的数值仿真

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上海交通大学学报 2005年第6期39卷 955-959页

作者：李如强陈进刘元峰上海交通大学振动冲击噪声国家重点实验室上海200030

针对从系统时间响应谱分析的角度研究转子碰磨故障的识别和诊断问题,提出了一种新的基于多尺度奇异谱分析的故障诊断方法.该方法首先建立转子碰磨模型,并用龙格-库塔法求解碰磨转子的运动方程得到碰磨转子的时间响应;其次构造了一种简... 详细信息

针对从系统时间响应谱分析的角度研究转子碰磨故障的识别和诊断问题,提出了一种新的基于多尺度奇异谱分析的故障诊断方法.该方法首先建立转子碰磨模型,并用龙格-库塔法求解碰磨转子的运动方程得到碰磨转子的时间响应;其次构造了一种简单的多尺度奇异谱分析方法,用不同尺度下的各阶经验正交函数作为分析函数;最后用提出的多尺度奇异谱分析方法分析了碰磨转子和正常转子的时间响应信号.数值仿真结果表明,具有数据自适应的分析函数能够捕获信号的不同特征,通过比较不同阶次的分析函数分析的结果,可以有效地对转子碰磨进行识别和诊断.

关键词：转子碰磨故障诊断多尺度奇异谱分析数值仿真

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Investigation of bubbles escape behavior from low basicity mold flux for high-Mn high-Al steels using 3D X-ray microscope

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International Journal of Minerals,Metallurgy and Materials 2025年第1期32卷 102-110页

作者： qiang liu Xiang li Shen Du Ming Gao Yanbin Yin Jiongming Zhang State Key Laboratory of Advanced Metallurgy University of Science and Technology BeijingBeijing 100083China

During the continuous casting process of high-Mn high-Al steels,various types of gases such as Ar need to escape through the top of the *** which,the behavior of bubbles traversing the liquid slag serves as a restrictive link,closely associated with viscosity and the thickness of liquid *** contrast to two-dimensional surface observation,three-dimensional(3D)analysis method can offer a more intuitive,accurate,and comprehensive ***,this study employs a 3D X-ray microscope(3D-XRM)to obtained spatial distribution and 3D morphological characteristics of residual bubbles in mold flux under different basicity of liquid slag,different temperatures,and different holding *** results indicate that as basicity of slag increases from 0.52 to 1.03,temperature increases from 1423 to 1573 K,the viscosity of slag decreases,the floating rate of bubbles *** addition,when holding time increases from 10 to 30 s,the bubbles floating distance increases,and the volume fraction and average equivalent sphere diameter of the bubbles solidified in the mold flux gradually *** one word,increasing the basicity,temperature,and holding time leading to an increase in the removal rate of bubbles especially for the *** findings of bubbles escape behavior provide valuable insights into optimizing low basicity mold flux for high-Mn high-Al steels.

关键词： mold flux low basicity bubbles three-dimensional X-ray microscope viscosity

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Effects of the extrusion parameters on microstructure,texture and room temperature mechanical properties of extruded Mg-2.49Nd-1.82Gd-0.2Zn-0.2Zr alloy

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International Journal of Minerals,Metallurgy and Materials 2025年第1期32卷 136-146页

作者： Chenjin Zhang Guangyu Yang Lei Xiao Zhiyong Kan Jing Guo qiang li Wanqi Jie State Key Laboratory of Solidification Processing Northwestern Polytechnical UniversityXi’an 710072China College of Materials Science and Engineering Fuzhou UniversityFuzhou 350108China College of Chemistry and Environmental Science Qujing Normal UniversityQujing 655011China

Microstructure,texture,and mechanical properties of the extruded Mg-2.49Nd-1.82Gd-0.2Zn-0.2Zr alloy were investigated at different extrusion temperatures(260 and 320℃),extrusion ratios(10:1,15:1,and 30:1),and extrusion speeds(3 and 6 mm/s).The experimental results exhibited that the grain sizes after extrusion were much finer than that of the homogenized alloy,and the second phase showed streamline distribution along the extrusion direction(ED).With extrusion temperature increased from 260 to 320℃,the microstructure,texture,and mechanical properties of alloys changed *** dynamic recrystallization(DRX)degree and grain sizes enhanced as the extrusion ratio increased from 10:1 to 30:1,and the strength gradually decreased but elongation(EL)*** the extrusion speed increased from 3 to 6 mm/s,the grain sizes and DRX degree increased significantly,and the samples presented the typical-rare-earth(RE)*** alloy extruded at 260℃ with extrusion ratio of 10:1 and extrusion speed of 3 mm/s showed the tensile yield strength(TYS)of 213 MPa and EL of 30.6%.After quantitatively analyzing the contribution of strengthening mechanisms,it was found that the grain boundary strengthening and dislocation strengthening played major roles among strengthening *** results provide some guidelines for enlarging the industrial application of extruded Mg-RE alloy.

关键词： Mg-rare earth alloys extrusion temperature extrusion ratio extrusion speed strengthening mechanisms

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基于知识的模糊神经网络的旋转机械故障诊断

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应用数学和力学 2006年第1期27卷 89-97页

作者：李如强陈进伍星上海交通大学振动冲击噪声国家重点实验室上海200030

提出了一种基于知识的模糊神经网络并用于故障诊断.首先基于粗糙集对样本数据进行初步规则获取,并计算规则的依赖度和条件覆盖度,然后根据规则数目进行模糊神经网络结构部分设计,规则的依赖度和条件覆盖度用于设定网络初始权重,而用遗... 详细信息

提出了一种基于知识的模糊神经网络并用于故障诊断.首先基于粗糙集对样本数据进行初步规则获取,并计算规则的依赖度和条件覆盖度,然后根据规则数目进行模糊神经网络结构部分设计,规则的依赖度和条件覆盖度用于设定网络初始权重,而用遗产算法对神经网络输出参数进行优化.这样的模糊神经网络称为基于知识的模糊神经网络.使用该网络对旋转机械常见故障进行诊断,结果表明,和一般模糊神经网络相比,该网络具有训练时间短而诊断率高的特点.

关键词：旋转机械故障诊断粗糙集模糊集遗传算法基于知识的模糊神经网络

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Gamma-glutamyl transferase 5 overexpression in cerebrovascular endothelial cells improves brain pathology,cognition,and behavior in APP/PS1 mice

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Neural Regeneration Research 2025年第2期20卷 533-547页

作者： Yanli Zhang Tian li Jie Miao Zhina Zhang Mingxuan Yang Zhuoran Wang Bo Yang Jiawei Zhang Haiting li qiang Su Junhong Guo Department of Neurology First Hospital of Shanxi Medical UniversityTaiyuanShanxi ProvinceChina Department of Neurology Sixth Hospital of Shanxi Medical University(General Hospital of Tisco)TaiyuanShanxi ProvinceChina Department of Physiology Key Laboratory of Cellular PhysiologyMinistry of EducationShanxi Medical UniversityTaiyuanShanxi ProvinceChina Department of Hernia and Abdominal Wall Surgery Shanxi Bethune HospitalShanxi Academy of Medical SciencesTongji Shanxi HospitalThird Hospital of Shanxi Medical UniversityTaiyuanShanxi ProvinceChina Department of Laboratory Medicine of Fenyang College Shanxi Medical UniversityFenyangShanxi ProvinceChina

In patients with Alzheimer’s disease,gamma-glutamyl transferase 5(GGT5)expression has been observed to be downregulated in cerebrovascular endothelial ***,the functional role of GGT5 in the development of Alzheimer’s disease remains *** study aimed to explore the effect of GGT5 on cognitive function and brain pathology in an APP/PS1 mouse model of Alzheimer’s disease,as well as the underlying *** observed a significant reduction in GGT5 expression in two in vitro models of Alzheimer’s disease(Aβ_(1-42)-treated hCMEC/D3 and bEnd.3 cells),as well as in the APP/PS1 mouse ***,injection of APP/PS1 mice with an adeno-associated virus encoding GGT5 enhanced hippocampal synaptic plasticity and mitigated cognitive ***,increasing GGT5 expression in cerebrovascular endothelial cells reduced levels of both soluble and insoluble amyloid-βin the brains of APP/PS1 *** effect may be attributable to inhibition of the expression ofβ-site APP cleaving enzyme 1,which is mediated by nuclear factor-kappa *** findings demonstrate that GGT5 expression in cerebrovascular endothelial cells is inversely associated with Alzheimer’s disease pathogenesis,and that GGT5 upregulation mitigates cognitive deficits in APP/PS1 *** findings suggest that GGT5 expression in cerebrovascular endothelial cells is a potential therapeutic target and biomarker for Alzheimer’s disease.