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Transfer RNA-derived fragment tRF-23-Q99P9P9NDD promotes progression of gastric cancer by targeting ACADSB

Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2024年第5期25卷 438-450页

作者： Yu ZHANG Xinliang GU Yang li Xun li Yuejiao huang Shaoqing JU Medical School of Nantong University Nantong UniversityNantong 226001China Department of Laboratory Medicine Affiliated Hospital of Nantong UniversityNantong 226001China Research Center of Clinical Medicine Afiliated Hospital of Nantong UniversityNantong 226001China Department of Medical Oncology Afiliated Hospital of Nantong UniversityNantong 226001China

Gastric cancer(GC)is one of the most common gastrointestinal tumors.As a newly discovered type of non-coding RNAs,transfer RNA(tRNA)-derived small RNAs(tsRNAs)play a dual biological role in cancer.Our previous studies have demonstrated the potential of tRF-23-Q99P9P9NDD as a diagnostic and prognostic biomarker for GC.In this work,we confirmed for the first time that tRF-23-Q99P9P9NDD can promote the proliferation,migration,and invasion of GC cells in vitro.The dual luciferase reporter gene assay confirmed that tRF-23-Q99P9P9NDD could bind to the 3'untranslated region(UTR)site of acyl-coenzyme A dehydrogenase short/branched chain(ACADSB).In addition,ACADSB could rescue the effect of tRF-23-Q99P9P9NDD on GC cells.Next,we used Gene Ontology(GO),the Kyoto Encyclopedia of Genes and Genomes(KEGG),and Gene Set Enrichment Analysis(GSEA)to find that downregulated ACADSB in GC may promote lipid accumulation by inhibiting fatty acid catabolism and ferroptosis.Finally,we verified the correlation between ACADSB and 12 ferroptosis genes at the transcriptional level,as well as the changes in reactive oxygen species(ROS)levels by flow cytometry.In summary,this study proposes that tRF-23-Q99P9P9NDD may affect GC lipid metabolism and ferroptosis by targeting ACADSB,thereby promoting GC progression.It provides a theoretical basis for the diagnostic and prognostic monitoring value of GC and opens upnew possibilities for treatment.

关键词： Transfer RNA(tRNA)-derived small RNA(tsRNA) Gastric cancer(GC) Acyl-coenzyme A dehydrogenase short/branched chain(ACADSB) Molecular mechanism Treatment Ferroptosis

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Autophagy in hepatic progenitor cells modulates exosomal miRNAs to inhibit liver fibrosis in schistosomiasis

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Frontiers of Medicine 2024年第3期18卷 538-557页

作者： Yue Yuan Jiaxuan li Xun Lu Min Chen Huifang liang Xiao-ping Chen Xin Long Bixiang Zhang Song Gong Xiaowei huang Jianping Zhao Qian Chen Division of Gastroenterology Department of Internal Medicine at Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan430030China Hepatic Surgery Center Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan430030China Hubei Key Laboratory of Hepato–Pancreato–Biliary Diseases Wuhan430030China Department of Trauma Surgery Tongji Trauma CenterTongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan430030China

Schistosoma infection is one of the major causes of liver fibrosis.Emerging roles of hepatic progenitor cells(HPCs)in the pathogenesis of liver fibrosis have been identified.Nevertheless,the precise mechanism underlying the role of HPCs in liver fibrosis in schistosomiasis remains unclear.This study examined how autophagy in HPCs affects schistosomiasis-induced liver fibrosis by modulating exosomal miRNAs.The activation of HPCs was verified by immunohistochemistry(IHC)and immunofluorescence(IF)staining in fibrotic liver from patients and mice with Schistosoma japonicum infection.By coculturing HPCs with hepatic stellate cells(HSCs)and assessing the autophagy level in HPCs by proteomic analysis and in vitro phenotypic assays,we found that impaired autophagy degradation in these activated HPCs was mediated by lysosomal dysfunction.Blocking autophagy by the autophagy inhibitor chloroquine(CQ)significantly diminished liver fibrosis and granuloma formation in S.japonicum-infected mice.HPC-secreted extracellular vehicles(EVs)were further isolated and studied by miRNA sequencing.miR-1306-3p,miR-493-3p,and miR-34a-5p were identified,and their distribution into EVs was inhibited due to impaired autophagy in HPCs,which contributed to suppressing HSC activation.In conclusion,we showed that the altered autophagy process upon HPC activation may prevent liver fibrosis by modulating exosomal miRNA release and inhibiting HSC activation in schistosomiasis.Targeting the autophagy degradation process may be a therapeutic strategy for liver fibrosis during Schistosoma infection.

关键词： schistosomiasis hepatic progenitor cell autophagy extracellular vesicle fibrosis miRNA

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P2P Energy Trading Considering Prosumer Preference and System Risk

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CSEE Journal of Power and Energy Systems 2024年第3期10卷 1179-1194页

作者： Xinyu He ping Dong Mingbo liu Xuewei huang Wenli Deng Ruijin He School of Electric Power Engineering South China University of TechnologyGuangzhou 510640China IEEE

As an important means of mobilizing demand-side resources,peer-to-peer(P2P)energy trading has drawn more and more attention from scholars.This paper constructs a P2P energy trading framework considering prosumers’trading partner preferences(TPPs)and system risk.At first,we build the P2P trading models of prosumers equipped with different distributed energy resources(DERs),and TPP models.Secondly,to solve the established energy trading problem,a fully distributed double-consensus alternating direction method of multipliers(DC-ADMM)is proposed,which can achieve transaction consensus when considering market players’TPPs.Then,a risk-based security constrained economic dispatch(RBSCED)model based on AC power flow is established for the first time,by which a distribution system operator(DSO)checks system security and obtains risk-based locational marginal prices(RLMPs).Moreover,double-regulated price signals related to RLMPs which contain grid utilization prices(GUPs)and DSO’s retail prices realize management of players’transactions.In the end,the proposed method is applied to an IEEE33 bus distribution system.Results show the proposed method effectively reduces system risk and ensures secure operation of system without direct management.

关键词： Double-consensus ADMM grid utilization price price signal risk-based locational marginal price trading partner preference

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Estimating the optimal vegetation coverage for the dominant tree and shrub species over China's northwest drylands

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Science China Earth Sciences 2024年第5期67卷 1500-1517页

作者： Zhongdian ZHANG Xiaoxu JIA ping ZHU Mingbin huang lidong REN Ming’an SHAO School of Geographic Sciences Xinyang Normal UniversityXinyang 464000China Key Laboratory of Ecosystem Network Observation and Modeling Institute of Geographic Sciences and Natural Resources ResearchChinese Academy of SciencesBeijing 100101China College of Resources and Environment University of Chinese Academy of SciencesBeijing 100190China Yellow River Delta Modern Agricultural Engineering Laboratory Chinese Academy of SciencesBeijing 100101China State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau Institute of Soil and Water ConservationNorthwest A&F UniversityYangling 712100China

Anthropogenic revegetation is an effective way to control soil erosion and restore degraded ecosystems in China's northwest drylands(NWD).However,excessive vegetation cover expansion has long been known to increase evapotranspiration,leading to reduced local water availability,which can in turn threaten the health and services of restored ecosystems.Determining the optimal vegetation coverage(OVC)is critical for balancing the trade-off between plant growth and water consumption in water-stressed areas,yet quantitative assessments over the entire NWD are still lacking.In this study,a modified Biome BioGeochemical Cycles(Biome-BGC)model was used to simulate the long-term(1961–2020)dynamics of actual evapotranspiration(ET_(a)),net primary productivity(NPP),and leaf area index(LAI)for the dominant non-native tree(R.pseudoacacia and P.sylvestris)and shrub(C.korshinkii and H.rhamnoides)species at 246 meteorological sites over NWD.The modified model incorporated the Richards equation to simulate transient unsaturated water flow in a multilayer soil module,and both soil and eco-physiological parameters required by the model were validated using field-observed ETadata for each species.Spatial distributions of OVC(given by the mean maximum LAI,LAI_(max))for the dominant species were determined within three hydrogeomorphic sub-areas(i.e.,the loess hilly-gully sub-area,the windy and sandy sub-area,and the desert sub-area).The modified Biome-BGC model performed well in terms of simulating ET_(a) dynamics for the four plant species.Spatial distributions of mean ET_a,NPP,and LAI_(max)generally exhibited patterns similar to mean annual precipitation(MAP).In the loess hilly-gully sub-area(MAP:210 to 710 mm),the OVC respectively ranged from 1.7 to 2.9 and 0.8 to 2.9 for R.pseudoacacia and H.rhamnoides.In the windy and sandy sub-area(MAP:135 to 500 mm),the OVC ranged from 0.3 to 3.3,0.5 to 2.6 and 0.6 to 2.1for P.sylvestris,C.korshinkii and H.rhamnoides,respectively.In the desert sub-area(MAP:90 to 500 mm),the OVC ranged from 0.4 t

关键词： Optimal vegetation coverage Water balance Leaf area index Biome-BGC model Dryland

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Extracorporeal membrane oxygenation as a bridging therapy for patients with thiamine deficiency-induced pulmonary arterial hypertension

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World Journal of Emergency Medicine 2024年第2期15卷 147-149页

作者： Fangfang Qiu Weixing Dai Tengyue huang Min li Department of Intensive Care Unit the Fourth Affiliated Hospital of Zhejiang University School of MedicineYiwu 322000China Department of Intensive Care Unit the Second Affiliated Hospital of Zhejiang University School of MedicineHangzhou 310009China

Thiamine deficiency is characterized by a deficiency of thiamine(vitamin B1),lactic acidosis,pulmonary arterial hypertension(PAH),right-sided heart failure,and peripheral nerve damage.Thiamine deficiency is easily misdiagnosed and missed during clinical practice and is associated with death in severe cases.[1,2]Here we reported a patient who was admitted to the hospital with severe metabolic acidosis and pulmonary hypertension and finally diagnosed with thiamine deficiency by detecting plasma vitamin B1 levels.Since clinically obvious thiamine deficiency is rare,and cases of thiamine deficiency requiring extracorporeal membrane oxygenation(ECMO)treatment are rarer,we believe that our case will be helpful for emergency clinicians.

关键词： vitamin hypertension arterial

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Endovascular treatment of ruptured lobulated anterior communicating artery aneurysms:A retrospective study of 24 patients

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World Journal of Clinical Cases 2024年第15期12卷 2529-2541页

作者： Sheng-Xuan huang Xun-ping Ai Ze-Hui Kang Zhi-Yong Chen Ren-Man li Zu-Chao Wu Feng Zhu Department of Neurosurgery Sanming First Hospital Affiliated to Fujian Medical UniversitySanming 365000Fujian ProvinceChina

BACKGROUND Lobulated intracranial aneurysm is a special type of aneurysm with at least one additional cyst in the neck or body of the aneurysm.Lobulated intracranial aneurysm is a complex aneurysm with complex morphology and structure and weak tumor wall,which is an independent risk factor for rupture and hemorrhage.Lobular aneurysms located in the anterior communicating artery complex account for 36.9%of all intracranial lobular aneurysms.Due to its special anatomical structure,both craniotomy and endovascular treatment are more difficult.Compared with single-capsule aneurysms,craniotomy for lobular intracranial aneurysms has a higher risk and complication rate.AIM To investigate the efficacy and safety of endovascular treatment for ruptured lobulated anterior communicating artery aneurysm(ACoAA).METHODS Patients with ruptured lobulated ACoAA received endovascular treatment in Sanming First Hospital Affiliated to Fujian Medical University from June 2020 to June 2022 were retrospectively included.Their demographic,clinical and imaging characteristics,endovascular treatment methods and follow-up results were collected.RESULTS A total of 24 patients with ruptured lobulated ACoAA were included,including 9 males(37.5%)and 15 females(62.5%).Their age was 56.2±8.9 years old(range 39-74).The time from rupture to endovascular treatment was 10.9±12.5 h.The maximum diameter of the aneurysms was 5.1±1.0 mm and neck width were 3.0±0.7 mm.Nineteen patients(79.2%)were double-lobed and 5(20.8%)were multilobed.Fisher's grade:Grade 2 in 16 cases(66.7%),grade 3 in 6 cases(25%),and grade 4 in 2 cases(8.3%).Hunt-Hess grade:Grade 0-2 in 5 cases(20.8%),grade 3-5 in 19 cases(79.2%).Glasgow Coma Scale score:9-12 in 14 cases(58.3%),13-15 in 10 cases(41.7%).Immediately postprocedural Raymond-Roy grade:grade 1 in 23 cases(95.8%),grade 2 in 1 case(4.2%).Raymond-Roy grade in imaging follow-up for 2 wk to 3 months:grade 1 in 23 cases(95.8%),grade 2 in 1 case(4.2%).Followup for 2 to 12 months showed that 21 patients(87.5%)had good functional o

关键词： Intracranial aneurysm Anterior cerebral artery Endovascular surgery Embolism Treatment outcome

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Rapid prediction of damaged ship roll motion responses in beam waves based on stacking algorithm

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Journal of Hydrodynamics 2024年第2期36卷 394-405页

作者： Xin-ran liu Ting-qiu li Zi-ping Wang School of Naval Architecture Ocean and Energy Power EngineeringWuhan University of TechnologyWuhan430063China

Accurate modeling for highly non-linear coupling of a damaged ship with liquid sloshing in waves is still of considerable interest within the computational fluid dynamics(CFD)and AI framework.This paper describes a data-driven Stacking algorithm for fast prediction of roll motion response amplitudes in beam waves by constructing a hydrodynamics model of a damaged ship based on the dynamic overlapping grid CFD technology.The general idea is to optimize various parameters varying with four types of classical base models like multi-layer perception,support vector regression,random forest,and hist gradient boosting regression.This offers several attractive properties in terms of accuracy and efficiency by choosing the standard DTMB 5415 model with double damaged compartments for validation.It is clearly demonstrated that the predicted response amplitude operator(RAO)in the regular beam waves agrees well with the experimental data available,which verifies the accuracy of the established damaged ship hydrodynamics model.Given high-quality CFD samples,therefore,implementation of the designed Stacking algorithm with its optimal combination can predict the damaged ship roll motion amplitudes effectively and accurately(e.g.,the coefficient of determination 0.9926,the average absolute error 0.0955 and CPU 3s),by comparison of four types of typical base models and their various forms.Importantly,the established Stacking algorithm provides one potential that can break through problems involving the time-consuming and low efficiency for large-scale lengthy CFD simulations.

关键词： Ship motion damaged ship computational fluid dynamics(CFD) machine learning stacking algorithm

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Structural and functional connectivity of the whole brain and subnetworks in individuals with mild traumatic brain injury:predictors of patient prognosis

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Neural Regeneration Research 2024年第7期19卷 1553-1558页

作者： Sihong huang Jungong Han Hairong Zheng Mengjun li Chuxin huang Xiaoyan Kui Jun liu Department of Radiology The Second Xiangya HospitalCentral South UniversityChangshaHunan ProvinceChina Department of Computer Science Aberystwyth UniversityAberystwythCeredigionUK Shenzhen Institutes of Advanced Technology Chinese Academy of SciencesShenzhenGuangdong ProvinceChina School of Computer Science and Engineering Central South UniversityChangshaHunan ProvinceChina Department of Radiology Quality Control Center of Hunan ProvinceChangshaHunan ProvinceChina Clinical Research Center for Medical Imaging of Hunan Province ChangshaHunan ProvinceChina

Patients with mild traumatic brain injury have a diverse clinical presentation,and the underlying pathophysiology remains poorly understood.Magnetic resonance imaging is a non-invasive technique that has been widely utilized to investigate neuro biological markers after mild traumatic brain injury.This approach has emerged as a promising tool for investigating the pathogenesis of mild traumatic brain injury.G raph theory is a quantitative method of analyzing complex networks that has been widely used to study changes in brain structure and function.However,most previous mild traumatic brain injury studies using graph theory have focused on specific populations,with limited exploration of simultaneous abnormalities in structural and functional connectivity.Given that mild traumatic brain injury is the most common type of traumatic brain injury encounte red in clinical practice,further investigation of the patient characteristics and evolution of structural and functional connectivity is critical.In the present study,we explored whether abnormal structural and functional connectivity in the acute phase could serve as indicators of longitudinal changes in imaging data and cognitive function in patients with mild traumatic brain injury.In this longitudinal study,we enrolled 46 patients with mild traumatic brain injury who were assessed within 2 wee ks of injury,as well as 36 healthy controls.Resting-state functional magnetic resonance imaging and diffusion-weighted imaging data were acquired for graph theoretical network analysis.In the acute phase,patients with mild traumatic brain injury demonstrated reduced structural connectivity in the dorsal attention network.More than 3 months of followup data revealed signs of recovery in structural and functional connectivity,as well as cognitive function,in 22 out of the 46 patients.Furthermore,better cognitive function was associated with more efficient networks.Finally,our data indicated that small-worldness in the acute stage could serve as a predictor of longitudinal ch

关键词： cognitive function cross-section follow-up functional connectivity graph theory longitudinal study mild traumatic brain injury prediction small-worldness structural connectivity subnetworks whole brain network

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Overexpression of TRPV1 activates autophagy in human lens epithelial cells under hyperosmotic stress through Ca^(2+)-dependent AMPK/mTOR pathway

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International Journal of Ophthalmology(English edition) 2024年第3期17卷 420-434页

作者： liu-Hui huang Jiao Lyu Sheng Chen Ting-Yi liang Yu-Qing Rao ping Fei Jing li Hai-Ying Jin Pei-Quan Zhao Department of Ophthalmology Shanghai Xinhua Hospital Affiliated with Shanghai Jiao Tong University School of MedicineShanghai 200092China Department of Ophthalmology Shanghai Tenth People’s Hospital Affiliated with Shanghai Tongji University School of MedicineShanghai 200072China Centre for Eye Research Australia Royal Victorian Eye and Ear HospitalEast MelbourneVictoria 3002Australia Ophthalmology Department of SurgeryUniversity of MelbourneEast MelbourneVictoria 3002Australia Shenzhen Eye Hospital Shenzhen Key Laboratory of OphthalmologyAffiliated Shenzhen Eye Hospital of Jinan UniversityShenzhen 518040Guangdong ProvinceChina Department of Ophthalmology Shanghai East HospitalSchool of MedicineTongji UniversityShanghai 200120China

●AIM:To explore whether autophagy functions as a cellular adaptation mechanism in lens epithelial cells(LECs)under hyperosmotic stress.●METHODS:LECs were treated with hyperosmotic stress at the concentration of 270,300,400,500,or 600 mOsm for 6,12,18,24h in vitro.Polymerase chain reaction(PCR)was employed for the mRNA expression of autophagyrelated genes,while Western blotting detected the targeted protein expression.The transfection of stub-RFP-sens-GFPLC3 autophagy-related double fluorescence lentivirus was conducted to detect the level of autophagy flux.Scanning electron microscopy was used to detect the existence of autolysosome.Short interfering RNA of autophagy-related gene(ATG)7,transient receptor potential vanilloid(TRPV)1 overexpression plasmid,related agonists and inhibitors were employed to their influence on autophagy related pathway.Flow cytometry was employed to test the apoptosis and intracellular Ca^(2+)level.Mitochondrial membrane potential was measured by JC-1 staining.The cell counting kit-8 assay was used to calculate the cellular viability.The wound healing assay was used to evaluate the wound closure rate.GraphPad 6.0 software was utilized to evaluate the data.●RESULTS:The hyperosmotic stress activated autophagy in a pressure-and time-dependent manner in LECs.Beclin 1 protein expression and conversion of LC3B II to LC3B I increased,whereas sequestosome-1(SQSTM1)protein expression decreased.Transient Ca^(2+)influx was stimulated caused by hyperosmotic stress,levels of mammalian target of rapamycin(mTOR)phosphorylation decreased,and the level of AMP-activated protein kinase(AMPK)phosphorylation increased in the early stage.Based on this evidence,autophagy activation through the Ca^(2+)-dependent AMPK/mTOR pathway might represent an adaptation process in LECs under hyperosmotic stress.Hyperosmotic stress decreased cellular viability and accelerated apoptosis in LECs and cellular migration decreased.Inhibition of autophagy by ATG7 knockdown had similar results.TRPV1 overexpression increased auto

关键词： cataract posterior capsular opacification lens epithelial cell hyperosmotic stress autophagy apoptosis transient receptor potential vanilloid 1

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Exceptional points enhance sensing in silicon micromechanical resonators

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Microsystems & Nanoengineering 2024年第1期10卷 17-25页

作者： Man-Na Zhang Lei Dong li-Feng Wang Qing-An huang Key Laboratory of MEMS of the Ministry of Education Southeast UniversityNanjing 210096China

Exceptional points(EPs)have recently emerged as a new method for engineering the response of open physical systems,that is,systems that interact with the environment.The systems at the EPs exhibit a strong response to a small perturbation.Here,we show a method by which the sensitivity of silicon resonant sensors can be enhanced when operated at EPs.In our experiments,we use a pair of mechanically coupled silicon micromechanical resonators constituting a parity-time(PT)-symmetric dimer.Small perturbations introduced on the mechanically coupled spring cause the frequency to split from the EPs into the PT-symmetric regime without broadening the two spectrum linewidths,and this frequency splitting scales with the square root of the perturbation strength.The overall signal-tonoise ratio is still greatly enhanced,although the measured noise spectral density of the EP sensing scheme has a slight increase comparable to the traditional counterpart.Our results pave the way for resonant sensors with ultrahigh sensitivity.

关键词： resonant symmetric points

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