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KAI1 is a potential target for anti-metastasis in pancreatic cancer cells

World Journal of Gastroenterology 2008年第7期14卷 1126-1132页

作者： Jian-Hua Xu Xiao-Zhong Guo li-nan ren li-Chun Shao Min-Pei liu Department of Gastroenterology The General Hospital of Shenyang Military Command Shenyang 110016 Liaoning Province China

AIM: To investigate whether KAI1, as a metastasis suppressor gene, is associated with invasive and metastatic ability of pancreatic cancer ***: KAI1 gene was transfected into pancreatic cancer cell line MiaPaCa Ⅱ by liposomes selected with G418. Expression of transfected cells was measured by Western blotting, immunofluorescence and immunocytochemistry. Tumor cell invasion and metastatic ability were detected through gelatinase activity and reconstituted basement membrane (Matrigel) assay. pCMV-KAI1 was directly injected into the heterotopic human pancreatic adenocarcinoma successfully established in the groin of BALB/C nude mice, by subcutaneous injection of MiaPaCa Ⅱ pancreatic cancer cells. The statistical analysis between groups was determined by Student's two tailed t ***: By Western blotting, MiaPaCa Ⅱ cells transfected by KAI1 gene indicated KAI1 expression at approximately 29.1 kDa. Cytoplasm staining was positive and uniformly spread in transfected cancer cells, using immunohistochemistry and immunofluorescence. The most obvious difference was present after 30 h (MiaPaca Ⅱ 43.6 ± 9.42, pCMV-MiaPaca Ⅱ 44.8 ± 8.56, pCMV-KAI1-MiaPaca Ⅱ 22.0 ± 4.69, P lipofectamine 1.22 ± 0.31 in A group were lower than control 4.61 ± 1.98 and pCMV-KAI 11.67 ± 0.81. Analyses of metastases with and without KAI1 transfection in mice were different in liver and lung between controls 1.62 ± 0.39, 0.45 ± 0.09, pCMV-KAI 1.01 ± 0.27, 0.33 ± 0.09 and KAI1-lipofectamine 0.99 ± 0.21, 0.30 ± 0.09 respectively (P lity. KAI1 gene plays an important role in inhibiting metastasis of

关键词： KAI1 Pancreatic cancer cell line Transfection Immunocytochemistry Western blotting Immunofluorescence Gelatinolysis

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KAI1 inhibits lymphangiogenesis and lymphatic metastasis of pancreatic cancer in vivo

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Hepatobiliary & Pancreatic Diseases International 2014年第1期13卷 87-92页

作者： Xu liu Xiao-Zhong Guo Hong-Yu li Jiang Chen li-nan ren Chun-Yan Wu Department of Gastroenterology General Hospital of Shenyang Military Area

BACKGROUND: Several studies have shown that KAI1 inhibits tumor metastasis, but its mechanism is not clear. The present study aimed to determine the role of KAI1 in lymphatic metastasis, specifically in pancreatic cancer. METHODS: The KAI1 gene was transfected into the pancreatic cancer cell line MIA PaCa-2 and PANC-1 by using liposomes and selected by G418, and the protein was measured by Western blotting. After successful infection, the cell growth curve was studied by MTT, vascular endothelial growth factor C(VEGF-C) secretion by pancreatic cancer cell were measured by EliSA. The KAI1 and pCMV transfected MIA PaCa-2 cells were renamed as MIA PaCa-2-K and MIA PaCa-2-p. These two kinds of cells were injected into the subcuticular layer of nude mice; both tumor growth and metastasis through the lymphatic nodes were assessed. Lymphangiogenesis in tumors was measured by immunohistochemistry. RESULTS: The VEGF-C secretion was significantly reduced in MIA PaCa-2 cells compared with PANC-1 cells after being transfected with the KAI1 gene. The growth rate of subcutaneous tumors was similar after the injection of MIA PaCa-2-K, MIA PaCa-2, and MIA PaCa-2-p. MIA PaCa-2-K tumors showed slower lymphangiogenesis and lymph node metastasis compared with MIA PaCa-2 and MIA PaCa-2-p tumors. CONCLUSION: The overexpression of KAI1 inhibits the lymphangiogenesis and lymph node metastasis of MIA PaCa-2 pancreatic tumors.

关键词： KAI1 pancreatic cancer lymphatic metastasis lymphangiogenesis

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Monitoring microbial community structure and succession of an A/O SBR during start-up period using PCR-DGGE

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Journal of Environmental Sciences 2009年第2期21卷 223-228页

作者： WANG Xiuheng,ZHANG Kun,ren nanqi,li nan,ren lijiao State Key Laboratory of Urban Water Resource and Environment,Harbin Institute of Technology,Harbin 150090,China. State Key Laboratory of Urban Water Resource and Environment Harbin Institute of Technology Harbin 150090 China

Polymerase chain reaction （PCR）-denaturing gradient gel electrophoresis （DGGE） protocol was employed for revealing microbial community structure and succession in a sequential anaerobic and aerobic reactor performing enhanced biological phosphorus removal （EBPR） during start-up period. High phosphorus removal was achieved after 15 d. On day 30, phosphorus removal efficiency reached to 83.2% and the start-up was finished. DGGE profiles of periodical sludge samples showed that dominant microbial species were 19 OTUs （operational taxonomy units）. Unweighted pair-group method using arithmetic averages （UPGMA） clustering analysis revealed that rapid community succession correlated to low phosphorus removal rate and high phosphorus removal efficiency reflected on steady community structure. Sequencing results indicated that determined sequences （12 OTUs） belonged to Proteobacterium, Actinobacteria, Gemmatimonadales and unaffiliate group. Proteobacterium, Tetrasphaera elongate and Gemmatimonas aurantiaca may act important roles in phosphorus removal. With little amount as known glycogen accumulating organisms, Candidatus Competibacter phosphatis still at accumulating-phase had limited effect on microbial community structure. When climax community was obtained, dominant microbes were 14 OTUs. Microbes in a large amount were uncultured bacterium Thauera sp., uncultured y-Proteobacterium and Tetrasphaera elongata.

关键词： biological phosphorus removal polyphosphate accumulating organisms glycogen accumulating organisms

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New tumor-associated antigen SC6 in pancreatic cancer

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World Journal of Gastroenterology 2005年第48期11卷 7671-7675页

作者： Min-Pei liu Xiao-Zhong Guo Jian-Hua Xu Di Wang Hong-Yu li Zhong-Min Cui Jia-Jun Zhao li-nan ren Department of Experimental Medicine Northern Hospital Shenyang Liaoning Province China

AIM: To examine the concentration of a new antigen SC6 (SC6-Ag) recognized by monoclonal antibody (MAb)in patients with pancreatic cancer and other malignant or benign diseases and to understand whether SC6-Ag has any clinical significance in distinguishing pancreatic cancer from other gastrointestinal ***: Six hundred and ninety-five serum specimens obtained from 115 patients with pancreatic cancer, 154 patients with digestive cancer and 95patients with non-digestive cancer were used and classified in this study. Serum specimens obtained from 140 patients with benign digestive disease and 89 patients with non-benign digestive disease served as controls. Ascites was tapped from 16 pancreatic cancer patients, 19 hepatic cancer patients, 16 colonic cancer patients, 10 gastric cancer and 6 severe necrotic pancreatitis patients. The samples were quantitated by solid-phase radioimmunoassay. The cut-off values (CV)of 41, 80, and 118 U/mL were ***: The average intra- and interassay CV detected by immunoradiometric assay of SC6-Ag was 5.4% and 8.7%, respectively. The sensitivity and specificity were 73.0% and 90.9% respectively. The levels in most malignant and benign cases were within the normal upper limit. Among the 16 pancreatic cancer cases, the concentration of SC6-Ag in ascites was over the normal range in 93.8% patients. There was no significant difference in the concentration of *** expression of SC6-Ag in sera was significantly related to tumor differentiation. The concentration of SC6-Ag was higher in patients before surgery than after surgery. The specificity of SC6-Ag and CA19-9 was significantly higher than that of ultrasound and computer tomography (CT) in pancreatic cancer patients. Higher positive predictive values were indicated in 92.3% SC6-Ag and 88.5% CA19-9, but lower in 73.8% ultrasound and 76.2% ***: The combined test of SC6-Ag and CA19-9 may improve the diagnostic rate of primary cancer. The detection of

关键词： Tumor antigen SC6 Pancreatic neoplasm Immunoradiometric assay

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Effect of bax,bcl-2 and bcl-xL on regulating apoptosis in tissues of normal liver and hepatocellular carcinoma

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World Journal of Gastroenterology 2002年第6期8卷 1059-1062页

作者： Xiao-Zhong Guo Xiao-Dong Shao Min-Pei liu Jian-Hua Xu li-nan ren Jia-Jun Zhao Hong-Yu li Di Wang,Department of Gastroenterology,General Hospital of Shenyang Military Region,Shenyang 110016,liaoning Province,China Department of Gastroenterology General Hospital of Shenyang Military Region Liaoning Province China.

AIM: To investigate the expression of bax, bcl-2 and bcl-xL mRNA in the tissues of normal liver and hepatocellular carcinoma (HCC), and analyze the relationship between the expression of bax, bcl-2 and bcl-xL mRNA and clinical parameters of HCC ***: The expression of bax, bcl-2 and bcl-xL mRNA of normal liver and HCC was measured by Northern blot. Statistical analyses were made by t test and correlation ***: A very low mRNA level was indicated at bax,bcl-2 and bcl-xL in the HCC tissues in contrast to the tissues of normal liver by Northern blot analysis. The analyses of mRNA level revealed that HCC tissues exhibited a mean 7.6-fold decrease in bax, 4.2-fold in bcl-2 and 3.5-fold in bcl-xL in comparison with normal control tissues, respectively. Positive correlation was found between bax and bcl-xL (r=0.7061,P＜0.01). There was no significance between the mRNA expression of these three genes and age, gender, tumor differentiation and tumor stage of HCC patients. CONCLUSION: The results are consistent with the fact that apoptosis rarely occurs in normal livers but increases in HCC, indicating that bcl-2 and bcl-xL may play a very important role in regulating the apoptosis of normal liver and HCC.

关键词： bax bcl-2 bcl-xL 凋亡介导因子正常肝组织肝细胞癌组织细胞凋亡调节作用

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Boosting charge extraction and efficiency of inverted perovskite solar cells through coordinating group modification at the buffer layer/cathode interface

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nano Research 2025年第1期18卷 415-423页

作者： Zhiqing liang Ziqiu ren Ziyu Wang nan Yan ling li Bo Zhang Yanlin Song College of Chemistry Zhengzhou UniversityZhengzhou 450052China Key Laboratory of Applied Surface and Colloid Chemistry Ministry of Education Shaanxi Key Laboratory for Advanced Energy DevicesShaanxi Engineering Lab for Advanced Energy Technology School of Materials Science and EngineeringShaanxi Normal UniversityXi’an 710119China Key Laboratory of Green Printing Institute of ChemistryChinese Academy of Sciences(ICCAS)Beijing Engineering Research Center of Nanomaterials for Green Printing TechnologyNational Laboratory for Molecular Sciences(BNLMS)Beijing 100190China

In inverted perovskite solar cells (PSCs),effective modification of the interface between the metalcathode and electron transport layer (ETL) is crucial forachieving high performance and stability. Herein, sulfonatedbathocuproine, commonly known as disodium bathocuproinedisulfonate (BCDS), was employed as a cathode buffer layerto address the interfacial issues at the [6,6]-phenyl-C61-butyricacid methyl ester (PCBM)/Ag interface. BCDS possesses theability to form coordinate bonds with Ag electrodes. Theutilization of the BCDS buffer layer enhanced the chargeextraction capability at the cathode interface whilesimultaneously achieving interfacial defect passivation,improving interfacial contact and increasing the built-in electricfield. Consequently, a power conversion efficiency (PCE) of25.06% was achieved. Furthermore, owing to the excellent filmforminguniformity of BCDS on PCBM, the stability of thedevice was also improved. After storage in dry air for morethan 2000 h, the device maintained 96% of its initial efficiency. This work underscores the remarkable potential of tailoringcoordination groups to enhance charge extraction efficiency at the ETL-cathode interface, unveiling a promising newfrontier in buffer layer development and performance optimization strategies for PSCs.

关键词： perovskite solar cells buffer layer bathocuproine disulfonate coordination group modification electron transport layer(ETL)-cathode interface

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Iron-handling solute carrier SLC22A17 as a blood-brain barrier target after stroke

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Neural Regeneration Research 2025年第11期20卷 3207-3208页

作者： Xueqi ren Wenlu li Key Laboratory of Neuropharmacology and Translational Medicine of Zhejiang Province School of Pharmaceutical SciencesZhejiang Chinese Medical UniversityHangzhouZhejiang ProvinceChina Neuroprotection Research Laboratories Departments of Radiology and NeurologyMassachusetts General HospitalHarvard Medical SchoolBostonMAUSA

The pathophysiology of ischemic stroke is complex and multifactorial,involving various forms of cell death such as apoptosis,autophagy,and necrosis.A recent study suggests that oxidative and inflammatory stress can induce ferroptosis,a specialized form of cell death characterized by the accumulation of lipid peroxides dependent on intracellular iron overload(li and Jia,2023).

关键词： death specialized barrier

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崩落法纵向分区开采冒落危害防治技术

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金属矿山 2016年第9期45卷 62-65页

作者：李楠常帅任凤玉辽宁科技大学矿业工程学院辽宁鞍山114051 东北大学资源与土木工程学院辽宁沈阳110819

针对崩落采矿法纵向分区开采过程中冒落危害防治这一关键安全技术问题,以小汪沟铁矿在矿体延深方向划分为3个分区开采为依托,运用理论分析方法,指出了各分区冒落危害的主要种类,其中上位分区为落块与陷落危害,中位分区为冒落冲击气浪与... 详细信息

针对崩落采矿法纵向分区开采过程中冒落危害防治这一关键安全技术问题,以小汪沟铁矿在矿体延深方向划分为3个分区开采为依托,运用理论分析方法,指出了各分区冒落危害的主要种类,其中上位分区为落块与陷落危害,中位分区为冒落冲击气浪与落块危害,下位分区为落块危害。同时,依据3分区的位置特点及矿体条件,调整回采顺序以利用连续回采面积控制初始冒落形式,并针对冲击气浪、落块、陷落危害,分别提出作业点与冒落体边缘保持5 m以上安全距离和在出矿口预留6 m厚散体隔离垫层、严禁作业人员进入空区出矿、地表堆石隔离人员进入陷落区等技术措施,适应了不同冒落条件下的安全需求,现场应用效果良好,为崩落法纵向分区开采矿山提供了安全技术保障。

关键词：纵向分区开采崩落法冒落形式冒落危害防治

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核壳结构PS/PU纳米复合物的合成及其性能的研究

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石油化工 2003年第9期32卷 796-799页

作者：朱岩孙多先李楠蒋稳仁天津大学化工学院天津300072

采用种子乳液聚合法 ,合成出以聚氨酯 (PU)为壳、聚苯乙烯 (PS)为核的核壳结构聚苯乙烯 /聚氨酯 (PS/PU)纳米复合乳液。采用动态光散射、透射电镜、红外光谱等测试手段对其结构特征及粒子形态进行了研究 ,其核 /壳质量比对纳米复合乳液... 详细信息

采用种子乳液聚合法 ,合成出以聚氨酯 (PU)为壳、聚苯乙烯 (PS)为核的核壳结构聚苯乙烯 /聚氨酯 (PS/PU)纳米复合乳液。采用动态光散射、透射电镜、红外光谱等测试手段对其结构特征及粒子形态进行了研究 ,其核 /壳质量比对纳米复合乳液的性能有一定影响 ,随着核 /壳质量比的增加 ,复合乳液的粒径和紫外吸光度增大 ,电导率下降。

关键词：核壳结构聚氨酯 PS/PU纳米复合物苯乙烯种子乳液聚合聚苯乙烯

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曝气生物滤池深度处理印染废水技术研究现状及发展趋势

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染整技术 2017年第7期39卷 53-56页

作者：代学民李亚楠任淑萍河北建筑工程学院河北张家口075000

通过对国内外曝气生物滤池深度处理印染废水技术研究文献的查阅分析,剖析了国内外曝气生物滤池深度处理印染废水污染物质及曝气生物滤池组合工艺深度处理印染废水的研究现状,针对曝气生物滤池的滤料选择、运行方式选择、运行参数优化以... 详细信息

通过对国内外曝气生物滤池深度处理印染废水技术研究文献的查阅分析,剖析了国内外曝气生物滤池深度处理印染废水污染物质及曝气生物滤池组合工艺深度处理印染废水的研究现状,针对曝气生物滤池的滤料选择、运行方式选择、运行参数优化以及与其他工艺的组合提出了曝气生物滤池深度处理印染废水研究发展趋势。

关键词：曝气生物滤池深度处理技术印染废水应用现状发展趋势

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