检索结果-南通市图书馆

Reversal of the phenotype by K-ras^(val12) silencing mediated by adenovirus-delivered siRNA in human pancreatic cancer cell line Panc-1

在线全文

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2005年第6期11卷 831-838页

作者： li-mochen Huang-YingLe Ren-YiQin ManojKumar Zhi-YongDu Rui-JuanXia JingDeng DepartmentofSurgery TongjiHospitalTongjiMedicalCollegeHuazhongUniversityofScienceandTechnologyWuhan430030HubeiProvinceChina CollegeoflifeSciences WuhanUniversityWuhan430072HubeiProvinceChina CenterforBiotechnology CollegeofLifeSciencesFujianAgricultureandForestryUniversityFuzhou350002FujianProvinceChina

AIM: To investigate the in vitro antitumor effect of adenovirus-mediated small interfering RNAs (siRNAs) on pancreatic cancer and the associated mechanism. METHODS: A 63-nucleotide (nt) oligonucleotide encoding K-rasval12 and specific siRNA were introduced into pSilencer 3.1-H1, then the H1-RNA promoter and siRNA coding insert were subcloned into pAdTrack to get plasmid pAdTrackH1-Avasval12. After homologous recombination in bacteria and transfections of such plasmids into a mammalian packaging cell line 293, siRNA expressing adenovirus Adh1-K-rasval12 was obtained. Stable suppression of K-rasval12 was detected by Northern blot and Western blot. Apoptosis in Panc-1 cells was detected by flow cytometry. RESULTS: We obtained adenovirus AdHl-K-rasval12 carrying the pSilencer 3.1-H1 cassette, which could mediate gene silencing. Through siRNA targeted K-rasval12, the oncogenic phenotype of cancer cells was reversed. Flow cytometry showed that apoptotic index of Panc-1 cells was significantly higher in the AdH1-K-rasval12-treatment group (18.70% at 72 h post-infection, 49.55% at 96 h post-infection) compared to the control groups (3.47%, 3.98% at 72 and 96 h post-infection of AdH1-empty, respectively; 4.21%, 3.78% at 72 and 96 h post-infection of AdHl-p53, respectively) (Pline Panc-1 can be induced after AdH1-K-rasval12 infection. This kind of adenovirus based on RNAi might be a promising vector for cancer therapy.

关键词： Pancreatic cancer siRNA Adenovirus Phenotype

Fault Diagnosis and Reliable Configuration of Uncertain Strip Area Based on MPSO-SVM

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Fault Diagnosis and Reliable Configuration of Uncertain Stri...

HKUST-1单位点铜催化CO2/CS2成环耦合三氟甲基化反应

第40届中国控制会议

作者： mochen li Bo Yao Fuzhong Wang College of Mathematics and System Science Shenyang Normal University Department of Basic Education Shenyang Institute of Engineering

For a class of linear uncertain flight control systems,in terms of the strip region,the problem of actuator continuous gain fault and reliable control is *** the same time,in order to solve the difficulty of selecting parameters of support vector machine,a parameter optimization algorithm based on improved Particle Swarm Optimization（MPSO） is *** with grid search method,particle swarm optimization and genetic algorithm,this method has the advantages of fast convergence,short time consuming and high *** the problem that the pole information is difficult to obtain,the algorithm theory of pole observer is given to realize the real-time observation of unknown ***,the effectiveness of reliable controller design is further proved by numerical simulation.

关键词： reliable control support vector machine pole observer particle swarm optimization algorithm grid search method genetic algorithm

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

精细化工 2020年第9期37卷 1839-1846页

作者：臧瀚彬李莫尘张铁欣段春迎大连理工大学精细化工国家重点实验室辽宁大连116024

以烯丙基胺类化合物为底物,Togni′s ReagentⅡ〔1-(三氟甲基)-1,2-苯碘酰-3(1H)-酮〕为三氟甲基源,CO2或CS2为C1合成子,高稳定性金属有机框架HKUST-1的Cu2(O2C)4双轮桨节点的单位点铜为催化剂,在温和条件下耦合CO2/CS2成环与三氟甲基... 详细信息

以烯丙基胺类化合物为底物,Togni′s ReagentⅡ〔1-(三氟甲基)-1,2-苯碘酰-3(1H)-酮〕为三氟甲基源,CO2或CS2为C1合成子,高稳定性金属有机框架HKUST-1的Cu2(O2C)4双轮桨节点的单位点铜为催化剂,在温和条件下耦合CO2/CS2成环与三氟甲基化反应。对碱性添加剂、溶剂、反应温度、催化剂负载量等反应条件进行了优化。结果表明,在5%的HKUST-1〔以底物N-苄基-2-苯基丙烯-2-烯-1-胺(1a)的物质的量为基准计算得到。HKUST-1的添加量基于Cu2(O2C)4双轮桨节点(催化剂最简分子结构式为Cu2(BTC)4/3·2H2O,摩尔质量为439.28g/mol)的摩尔质量计算,下同〕、CO2压力为101.325kPa、45℃的最佳反应条件下,含不同取代基的底物能以69%~87%的产率得到三氟甲基-唑烷酮医药中间体。自由基捕获实验验证了该反应可能的反应机理。催化剂HKUST-1可循环使用4次仍保持良好催化活性和晶态结构。使用CS2替代CO2时,可得到具有中枢神经调节作用的三氟甲基-噻唑烷硫酮衍生物,体现出该体系对硫毒化的耐受性。

关键词：金属有机框架铜催化三氟甲基化二氧化碳二硫化碳 唑烷酮噻唑烷硫酮催化技术

基于GA-SVM梯形区域的故障诊断与可靠配置

在线全文

学校读者我要写书评

暂无评论

沈阳师范大学学报（自然科学版） 2021年第3期39卷 210-214页

作者：姚波李默臣王福忠沈阳师范大学数学与系统科学学院沈阳110034 沈阳工程学院基础教学部沈阳110136

针对一类线性定常飞控系统,基于梯形区域下,研究了执行器连续增益故障可靠控制的问题。同时,为解决支持向量机选取参量困难而导致故障信息难以识别等问题,提出实值编码遗传算法来实现支持向量机模型参数的自动寻优。与传统的支持向量机... 详细信息

针对一类线性定常飞控系统,基于梯形区域下,研究了执行器连续增益故障可靠控制的问题。同时,为解决支持向量机选取参量困难而导致故障信息难以识别等问题,提出实值编码遗传算法来实现支持向量机模型参数的自动寻优。与传统的支持向量机相比,该方法具有收敛速度快、能耗低、耗时短、迭代次数少等优点。对于未知系统极点信息难以获取的问题,给出了极点观测器的算法,实现了对极点数据的实时观测。仿真结果表明,依据不同通道发生故障时极点所处区域不同的特点而设计的可靠控制器,不但鲁棒性能好,而且准确率也较高。该方法与粒子群算法(PSO-SVM)、网格搜寻法(gridsearch)相比具有较好的泛化能力及较高的识别度。

关键词：遗传算法支持向量机极点观测器粒子群算法网格搜寻法

符合功能安全的电源管理方案在新能源商用车VCU中的应用

在线全文

学校读者我要写书评

暂无评论

时代汽车 2022年第2期 91-94页

作者：李漠尘熊建赵鑫龙宋屹璋北汽福田汽车股份有限公司北京市102206

本文介绍了一种应用在新能源商用车VCU上的电源管理方案,以一款符合车载功能安全(ISO26262标准和ASIL D等级)的电源管理芯片FS8510为核心,聚焦电源系统的芯片选型依据和软硬件设计方案,突出其在提升车载电控模块功能安全方面所做的措施。

关键词：新能源商用车 VCU 电源功能安全 FS8510

同方期刊数据库博看期刊评论

在线全文

学校读者我要写书评

暂无评论

《南湖画桥》

艺术科技 2020年第16期33卷 F0004-F0004页

作者：李默尘河源职业技术学院

Lanthanide-based ratiometric luminescence nanothermometry

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Nano Research 2023年第2期16卷 2949-2967页

作者： mochen Jia Xu Chen Ranran Sun Di Wu Xinjian li Zhifeng Shi Guanying Chen Chongxin Shan Key Laboratory of Materials Physics of Ministry of Education School of Physics and MicroelectronicsZhengzhou UniversityZhengzhou 450052China MIIT Key Laboratory of Critical Materials Technology for New Energy Conversion and Storage School of Chemistry and Chemical Engineering&Key Laboratory of Micro-systems and Micro-structuresMinistry of EducationHarbin Institute of TechnologyHarbin 150001China

Luminescent nanothermometry can precisely and remotely measure the internal temperature of objects at nanoscale precision,which,therefore,has been placed at the forefront of scientific *** particular,due to the high photochemical stability,low toxicity,rich working mechanisms,and superior thermometric performance,lanthanide-based ratiometric luminesencent thermometers are finding prevalent uses in integrated electronics and optoelectronics,property analysis of in-situ tracking,biomedical diagnosis and therapy,and wearable e-health *** recent progresses,it remains debate in terms of the underlying temperature-sensing mechanisms,the quantitative characterization of performance,and the reliability of temperature *** this review,we show the origin of thermal response luminescence,rationalize the ratiometric scheme or thermometric mechanisms,delve into the problems in the characterization of thermometric performance,discuss the universal rules for the quantitative comparison,and showcase the cutting-edge design and emerging applications of lanthanide-based ratiometric ***,we cast a look at the challenges and emerging opportunities for further advances in this field.

关键词： luminescent nanothermometry lanthanide ions ratiometric thermometers thermal imaging

The Global Landscape of SARS-CoV-2 Genomes, Variants, and Haplotypes in 2019nCoVR

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Genomics, Proteomics & Bioinformatics 2020年第6期18卷 749-759页

作者： Shuhui Song lina Ma Dong Zou Dongmei Tian Cuiping li Junwei Zhu Meili Chen Anke Wang Yingke Ma Mengwei li Xufei Teng Ying Cui Guangya Duan mochen Zhang Tong Jin Chengmin Shi Zhenglin Du Yadong Zhang Chuandong liu Rujiao li Jingyao Zeng lili Hao Shuai Jiang Hua Chen Dali Han Jingfa Xiao Zhang Zhang Wenming Zhao Yongbiao Xue Yiming Bao China National Center for Bioinformation Beijing 100101China National Genomics Data Center Beijing Institute of GenomicsChinese Academy of SciencesBeijing 100101China CAS Key Laboratory of Genome Sciences and Information Beijing Institute of GenomicsChinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100049China CAS Key Laboratory of Genomic and Precision Medicine Beijing Institute of GenomicsChinese Academy of SciencesBeijing 100101China Center for Excellence in Animal Evolution and Genetics Chinese Academy of SciencesKunming 650223China

On January 22,2020,China National Center for Bioinformation(CNCB)released the 2019 Novel Coronavirus Resource(2019nCoVR),an open-access information resource for the severe acute respiratory syndrome coronavirus 2(SARS-CoV-2).2019nCoVR features a comprehensive integration of sequence and clinical information for all publicly available SARS-CoV-2 isolates,which are manually curated with value-added annotations and quality evaluated by an automated in-house *** particular note,2019nCoVR offers systematic analyses to generate a dynamic landscape of SARS-CoV-2 genomic variations at a global *** provides all identified variants and their detailed statistics for each virus isolate,and congregates the quality score,functional annotation,and population frequency for each *** change for each variant can be visualized and historical viral haplotype network maps for the course of the outbreak are also generated based on all complete and high-quality genomes ***,2019nCoVR provides a full collection of SARS-CoV-2 relevant literature on the coronavirus disease 2019(COVID-19),including published papers from PubMed as well as preprints from services such as bioRxiv and medRxiv through Europe ***,by linking with relevant databases in CNCB,2019nCoVR offers data submission services for raw sequence reads and assembled genomes,and data sharing with ***,SARS-CoV-2 is updated daily to collect the latest information on genome sequences,variants,haplotypes,and literature for a timely reflection,making 2019nCoVR a valuable resource for the global research community.2019nCoVR is accessible at https://***/ncov/.

关键词： 2019nCoVR SARS-CoV-2 Database Genomic variation Haplotype

固态发酵饲料自动发酵饲喂一体设备设计与试验

在线全文

学校读者我要写书评

暂无评论

农业机械学报 2018年第11期49卷 141-147页

作者：刘莫尘马郡祥韩守强李承李玉道李法德山东农业大学机械与电子工程学院泰安271018 山东省园艺机械与装备重点实验室泰安271018

针对固态发酵饲料在饲喂现场生产的需求和特点,设计了自动发酵饲喂一体设备。该设备由上料发酵系统、控制系统、饲喂系统构成:上料机构将饲料和菌液混合均匀送至发酵桶,发酵完成后自动下料至饲喂车;饲喂车能够准确定位到不同饲喂对象的... 详细信息

针对固态发酵饲料在饲喂现场生产的需求和特点,设计了自动发酵饲喂一体设备。该设备由上料发酵系统、控制系统、饲喂系统构成:上料机构将饲料和菌液混合均匀送至发酵桶,发酵完成后自动下料至饲喂车;饲喂车能够准确定位到不同饲喂对象的食槽并进行定量布料;操作者只需在人机交互界面设置上料体积、发酵时长、饲喂对象等参数,整个发酵饲喂过程无需人工干预。饲喂系统由48 V/100 A·h的蓄电池供电,充电一次可连续工作4 d。试验结果表明,发酵桶上料和饲喂车排料的料体积误差均不大于6%,饲喂车定位误差平均值11. 75 mm,发酵时间、饲喂对象等参数控制准确。设备运行稳定可靠,发酵和饲喂之间无缝对接,显著减小了劳动强度,满足发酵饲料现场固态发酵并饲喂的要求。

关键词：固态发酵饲料自动上料智能饲喂