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Effect of JIANPI HUOxuE decoction on inflammatory cytokine secretion pathway in rat liver with lipopolysaccharide challenge

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World Journal of Gastroenterology 2008年第12期14卷 1851-1857页

作者： Jing-Hua Peng Yi-Yang Hu Yang Cheng Chong Han li-li xu Qin Feng Shao-Dong Chen Qing Tao Hong-Shan li xue-Mei li Institute of liver Diseases Shuguang HospitalShanghai University of Traditional Chinese Medicine Shanghai201203 China

AIM： To evaluate the effect of Chinese traditional medicinal prescription, JIANPI HUOxuE decoction （JHD） on cytokine secretion pathway in rat liver induced by lipopolysaccharide （LPS）. METHODS： Twenty-four male SD rats were divided into normal group （n = 4）, model group （n = 10） and JHD group （n = 10） randomly. Rats in model group and JHD group were administrated with normal saline or JHD via gastrogavage respectively twice a day for 3 d. One hour after the last administration, rats were injected with LPS via tail vein, 50 μg/kg. Simultaneously, rats in normal group were injected with equivalent normal saline. After LPS stimulation for 1.5 h, serum and liver tissue were collected. Pathological change of liver tissues was observed through hematoxylineosin （H.E.） staining. Tumor necrosis factor alpha （TNF-α） in serum were assayed by enzyme linked immunosorbent assay （EliSA）. The protein expression of TNF-α, phosphorylated inhibit-κB （p-κB） and CD68 in liver were assayed by Western blot. The distribution of CD68 protein in liver was observed through immunohistochemical staining. The mRNA expression of TNF-α, interleukin-6 （IL-6）, CD14, toll-like receptor 2 （TLR2） and TLR4 in liver were assayed by real-time ***： Predominant microvesicular change, hepatocyte tumefaction and cytoplasm dilution were observed in liver tissues after LPS administration as well as obvious CD68 positive staining in hepatic sinusoidal. After LPS stimulation, serum TNF-α （31.35 ± 6.06 vs 12225.40 ± 9007.03, P 〈 0.05）, protein expression of CD68 （1.13 ± 0.49 vs 3.36 ±1.69, P 〈 0.05）, p-IκB （0.01 ±0.01 vs 2.07 ＋ 0.83, P 〈 0.01） and TNF-α （0.27 ± 0.13 vs 1.29 ± 0.37, P 〈 0.01） in liver and mRNA expression of TNF-α （1.96 ± 2.23 vs 21.45 ±6.00, P 〈 0.01）, IL-6 （4.80 ± 6.42 vs 193.50 ± 36.36, P 〈 0.01） and TLR2 （1.44 ± 0.62 vs 4.16 ± 0.08, P 〈 0.01） in liver were also increased significantly. These pathological changes were all improved in .1HD group. On the other hand, TLR4 mRNA （1.22 ± 0.3

关键词： JIANPI HUOxuE decoction lipopolysaccharide Kupffer cell, Cytokine Endotoxin receptor

PSD95 Gene Specific siRNAs Attenuate Neuropathic Pain through Modulating Neuron Sensibility and Postsynaptic CaMKIIα Phosphorylation

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Chinese Medical Sciences Journal 2011年第4期26卷 201-207页

作者： Le Shen xu li Wen Chen li xu Wei liu xue-rong Yu Yu-guang Huang Department of Anesthesiology Peking Union Medical College Hospital Chinese Academy of Medical Sciences ＆ Peking Union Medical College Beijing 100730 China

Objective To observe the effects of PSD95 gene specific siRNAs on neuropathic pain relief, neuron viability, and postsynaptic calcium/calmodulin-dependent protein kinase IIα (CaMKIIα) phosphorylation in vitro and in vivo. Methods Gene-specific siRNAs of rat PSD95 were synthesized chemically for transfection. Adult male Sprague-Dawley (SD) rats were randomly divided into 3 groups: nave group (n=6), sham group (n=6), and sciatic nerve chronic constriction injury (CCI) group (n=24). The CCI group was further divided into 4 groups (n=6 in each group), which were pretreated with normal saline, transfection vehicle, negative control siRNAs, and PSD95 gene specific siRNAs respectively. All the subgroups received corresponding agents intrathecally for 3 days, started one day before the CCI of sciatic nerve. Both mechanical allodynia and thermal hyperalgesia were measured on post-operative day 3 and 7. PSD95 gene silenced NG108-15 cells were further stimulated by glutamate, with the cell viability and the expression/phosphorylation of CaMKIIα measured by MTT cell proliferation assay and Western blot, respectively. Results The siRNAs decreased PSD95 mRNA level significantly both in vivo and in vitro. Neuropathic pain rats pretreated with PSD95 gene specific siRNAs exhibited significant elevation in the mechanical withdrawal threshold and paw withdrawal thermal latency, without affecting the baseline nociception. PSD95 gene silencing enhanced neuronal tolerance against the glutamate excitotoxicity, meanwhile the phosphorylation of CaMKIIα Thr286 was attenuated. Conclusion Pre-emptive administration of PSD95 gene specific siRNAs may attenuate the central sensitization CaMKIIα-related signaling cascades, leading to the relief of neuropathic pain.

关键词： PSD95 siRNAs neuropathic pain glutamate excitotoxicity calcium/calmodulin- dependent protein kinase IItt

Condition Monitoring and Fault Diagnosis Based on Rough Set Theory

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仪器仪表学报 2005年第Z1期26卷 781-783页

作者： li Xiong li Shengli xu Zongchang Academy of Armored Force Engineering Beijing 100072China Academy of Armored Force Engineering Beijing 100072China Academy of Armored Force Engineering Beijing 100072China

In order to raise the efficiency,automatization and intelligentization of condition monitoring and fault diagnosis for complex equipment systems,rough set theory is used to the field. A feature reduction algorithm based on rough set theory is adopted to extract condition information in monitoring and diagnosis for an engine,so that the technology condition monitoring parameters are optimized. The decision tables for each fault source are built and the diagnosis rules rooting in rough set reduction is applied to carry through intelligent fault diagnosis. The cases studied show that rough set method in condition monitoring and fault diagnosis can lighten the work burden in feature selection and afford advantages for autonomic learning and decision during diagnosis.

关键词： Condition monitoring Fault diagnosis Rough set theory Engine

Evaluation of Sensitivities and Specificities of SARS-CoV Detection by Real-time Quantitative Reverse Transcription-PCR Assays

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Virologica Sinica 2009年第3期24卷 187-193页

作者： li-li xu Zhi-hong HU Hua-lin WANG Xiao HAN Fei DENG State Key Laboratory of Virology Wuhan Institute of Virology Chinese Academy of Sciences Wuhan 430071 China

The etiological agent of severe acute respiratory syndrome(SARS) was identified as a new coronavirus,termed SARS-CoV. Establishment of an efficient and sensitive diagnostic system of SARS-CoV genetic materials is crucial for SARS control. In this study,we quantified SARS-CoV mRNAs in both infected cell culture lysate and in supernatant by using Real-time quantitative revere transcription-PCR based on EvaGreenTM dye and Taqman-MGB probes. For extensive evaluation of sensitivities and specificities,13 pairs of primers and 4 probes were designed based on different genes of SARS-CoV. Glyceraldehydes-3-phosphate dehydrogenase(GAPDH) was selected as the internal control gene. Results showed that S-gene-specific PCR was the most sensitive for detection,but because of its sequence variability in the different viral strains,primers and a probe based on the N gene were suitable substitutions. Meanwhile,we found the mRNA concentrations in cell culture lysates were much higher than in cell supernatant and facilited more sensitive detection of the SARS-CoV.

关键词： SARS-CoV Sensitivities Specificities Evaluation

Aquaporin-4-IgG-seropositive neuromyelitis optica spectrum disorders:progress of experimental models based on disease pathogenesis

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Neural Regeneration Research 2025年第2期20卷 354-365页

作者： li xu Huiming xu Changyong Tang Department of Neurology The Third Affiliated Hospital of Sun Yat-sen UniversityGuangzhouGuangdong ProvinceChina

Neuromyelitis optica spectrum disorders are neuroinflammatory demyelinating disorders that lead to permanent visual loss and motor *** date,no effective treatment exists as the exact causative mechanism remains ***,experimental models of neuromyelitis optica spectrum disorders are essential for exploring its pathogenesis and in screening for therapeutic *** most patients with neuromyelitis optica spectrum disorders are seropositive for IgG autoantibodies against aquaporin-4,which is highly expressed on the membrane of astrocyte endfeet,most current experimental models are based on aquaporin-4-IgG that initially targets *** experimental models have successfully simulated many pathological features of neuromyelitis optica spectrum disorders,such as aquaporin-4 loss,astrocytopathy,granulocyte and macrophage infiltration,complement activation,demyelination,and neuronal loss;however,they do not fully capture the pathological process of human neuromyelitis optica spectrum *** this review,we summarize the currently known pathogenic mechanisms and the development of associated experimental models in vitro,ex vivo,and in vivo for neuromyelitis optica spectrum disorders,suggest potential pathogenic mechanisms for further investigation,and provide guidance on experimental model *** addition,this review summarizes the latest information on pathologies and therapies for neuromyelitis optica spectrum disorders based on experimental models of aquaporin-4-IgG-seropositive neuromyelitis optica spectrum disorders,offering further therapeutic targets and a theoretical basis for clinical trials.

关键词： aquaporin-4 experimental model neuromyelitis optica spectrum disorder pathogenesis

Multi-organ hereditary hemorrhagic telangiectasia:A case report

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World Journal of Clinical Cases 2023年第28期11卷 6831-6840页

作者： Ying-ling Chen Hong-Yue Jiang Dong-Ping li Jiang lin Yun Chen li-li xu Hong Gao Department of Gastroenterology and Hepatology Zhongshan HospitalFudan UniversityShanghai 200032China Department of Radiology Zhongshan HospitalFudan UniversityShanghai 200032China Shanghai Institute of Medical Imaging Shanghai 200032China

BACKGROUND Type 2 hereditary hemorrhagic telangiectasia(HHT)is a rare autosomal dominant disease and is associated with ALK1 gene *** 2 HHT patients primarily suffer from recurrent *** is currently no promising *** SUMMARY A 5-year-old Chinese patient(III23)was admitted to Zhongshan Hospital for recurrent melena occurring over 2 *** had been experiencing epistaxis for years and had been diagnosed with idiopathic pulmonary hypertension 4 mo before *** computed tomography examination showed hepatic arteriovenous *** testing revealed a c.1121G>A mutation on the ALK1 *** to the international diagnostic criteria,this patient was diagnosed with *** addition,8 more family members exhibited HHT symptoms to varying *** testing in 5 family members(2 with HHT symptoms and 3 without HHT symptoms)revealed the ALK1 c.1121G>A mutation in the 2 family members with HHT *** missense mutation results in the substitution of arginine for glutamine at amino acid position 374(R374Q)in the conserved functional kinase domain of *** studies revealed that this mutation decreased the kinase activity of ALK1 and impeded the phosphorylation of its substrate ***,the R374Q mutant downregulated the protein level of collagen-1,a fibrogenic factor,indicating abnormal fiber generation during vascular *** The R374Q mutant of ALK1 and its subsequent influence on fiber generation highly indicated its pathogenic role in this family with type 2 *** of this gene mutation will facilitate early diagnosis of suspected type 2 HHT patients,and mechanistic studies will provide insights for future therapy.

关键词： Hereditary hemorrhagic telangiectasia Pedigree ALK1 Gene mutation Case report

Fibrinogen-like protein 2/fibroleukin prothrombinase contributes to tumor hypercoagulability via IL-2 and IFN-γ

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World Journal of Gastroenterology 2008年第39期14卷 5980-5989页

作者： Kai Su Fang Chen Wei-Ming Yan Qi-li Zeng li xu Dong Xi Bin Pi Xiao-Ping Luo Qin Ning Department of Infectious Diseases and Pediatrics Institute of Infectious Diseases Tongji Hospital of Tongji Medical College Huazhong University of Science and Technology 1095 Jiefang Avenue Wuhan 430030 Hubei Province China

AIM： To examine the role of Fibrinogen-like protein 2 （fgl2）/fibroleukin in tumor development. Fgl2 has been reported to play a vital role in the pathogenesis in MHV-3 （mouse hepatitis virus） induced fulminant and severe hepatitis, spontaneous abortion, allo- and xenograft rejection by mediating ＂immune coagulation＂.METHODS： Tumor tissues from 133 patients with six types of distinct cancers and the animal tumor tissues from human hepatocellular carcinoma （HCC） model on nude mice （established from high metastasis HCC cell line MHCC97LM6） were obtained. RESULTS： HfgI2 was detected in tumor tissues from 127 out of 133 patients as well as tumor tissues collected from human HCC nude mice. Hfgl2 was highly expressed both in cancer cells and interstitial inflammatory cells including macrophages, NK cells, and CD8^＋ T lymphocytes and vascular endothelial cells. HfgI2 mRNA was localized in cells that expressed hfgI2 protein. Fibrin （nogen） colocalization with hfgl2 expression was determined by dual immunohistochemical staining. In vitro, IL-2 and IFN-γ, increased hfgl2 mRNA by 10-100 folds and protein expression in both THP-1 and HUVEC cell lines. One-stage clotting assays demonstrated that THP-1 and HUVEC cells expressing hfgl2 had increased procoagulant activity following cytokines stimulation. CONCLUSION： The hfgI2 contributes to the hypercoagulability in cancer and may induce tumor angiogenesis and metastasis via cytokine induction.

关键词： Fibrinogen-like protein 2/fibroleukin Thrombin Tumor Coagulation Cytokine

Uncarialines A-E,new alkaloids from Uncaria rhynchophylla and their anticoagulant activity

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Natural Products and Bioprospecting 2023年第1期13卷 598-606页

作者： Ke-Pu Huang li-li xu Sheng li Yin-ling Wei lian Yang Xiao-Jiang Hao Hong-Ping He Yu Zhang School of Chinese Materia Medica Yunnan University of Chinese MedicineKunming 650500People’s Republic of China State Key Laboratory of Phytochemistry and Plant Resources in West China Kunming Institute of BotanyChinese Academy of SciencesKunming 650201People’s Republic of China

Uncarialines A-E(1-5),five undescribed monoterpene indole alkaloids,together with five known analogues were obtained from the stems of Uncaria *** 1-3 were unique 3,4-seco-tricyclic alkaloids with a 6/5/10 ring system,while 4 and 5 possessed a rare rearranged scaffold originated from corynantheine-type alkaloids with C-2/C-7 *** structures were characterized by a comprehensive analysis of MS,NMR,and *** effects on blood clotting times of human plasma were evaluated and alkaloid 5 had a slight prolongation effect on both thrombin time and activated partial thromboplastin time(p<0.001).

关键词： Uncaria rhynchophylla Monoterpene indole alkaloids Uncarialines A-E Anticoagulant activity

Lung Cancer Survival Improvement through Surgical Intervention in PUMCH Hospital

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The Chinese-German Journal of Clinical Oncology 2006年第4期5卷 240-244页

作者： GUO Feng ZHANG Zhiyong CUI Yushang li Shanqing li li xu Xiaohui GE Feng GUO Huiqin li Zejian Department of Thoracic Surgery PUMC Hospital Beijing 100730 China

Objective： To investigate and evaluate improvement of lung cancer survival after surgical intervention in PUMC hospital during the last 15 years. Methods： From January 1989 to December 2003, 1574 lung cancer cases underwent surgical treatment and followed up. All cases in this series were divided into two groups according to time period： group A （1999-2003） and group B （1989-1998）. The difference in the survival rate between groups A and B was compared. Results： The morbidity and mortality in group A was decreased significantly in comparison to group B （11.2% vs. 19.2%, 1.06% vs. 1.93%, respectively）. However, the 3-year and 5-year survival rate was increased from 42.35% to 56.07%, and from 28.46% to 38.99%, respectively. A significant improvement in survival was observed in patients with stage Ⅰ, Ⅱ and ⅢA, but not in those with stage ⅢB and Ⅳ. Also, patients with lobectomy had more satisfactory results than those receiving exploratory thoracotomy, limited resection, pneumonectomy and sleeve resection. Conclusion： Lobectomy plus systematic mediastinal lymph nodes dissection has become the standard mode for resectable lung cancer. Combination of complete resection along with lymph nodal dissection, and postoperative adjuvant chemotherapy based on platinum/3^rd generation chemotherapy medicine, has preliminarily been justified, proving an important approach for effective improvement in long-term survival of non-small cell lung carcinoma.

关键词： lung neoplasm surgery lobectomy