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Changes of brain neuropeptide Y and its receptors in rats with flurazepam tolerance and dependence

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Acta Pharmacologica Sinica 2005年第11期26卷 1290-1296页

作者： li-ping ZHANG, li wang~2 Department of Pediatrics, Peking University First Hospital, Beijing 100034, China Department of Pediatrics Peking University First Hospital Beijing China

Aim: Anticonvulsant tolerance and dependence are two obstacles that restrict the clinical use of benzodiazepines (BDZ). In order to explore the mechanism of these two adverse reactions, changes of neuropeptide Y (NPY) and its receptors in the hippocampus of rat models, in relation to flurazepam (FZP, a member of BDZ) tolerance and dependence, were investigated. Methods: The mRNA of preproNPY and its receptors (Y1, Y2, and Y5) in the hippocampus were determined by competitive RT-PCR, and the distribution of NPY in the hippocampus was examined by immunohistochemistry. Results: A decrease of preproNPY mRNA in the hippocampus was found in tolerant and dependent rats. The level of preproNPY mRNA in the hippocampus was reversely correlated with the degree of tolerance and dependence, measured by the threshold of pentylenetetrazol-induced seizures. Immunohistochemistry indicated a decrease of NPY-immunoreactive material in neurons of the CA1, CA3, and dentate gyrus regions of both tolerant and dependent rats. The mRNA of NPY receptors Y,and Y5decreased in tolerant rats but did not change in dependent rats. The mRNA of NPY receptor Y2increased in tolerant rats but decreased in dependent rats. Conclusion: A decrease of NPY in the hippocampus might be involved in anticonvulsant tolerance and dependence following long-term treatment with FZP. Y,, Y2, and Y5mRNA were also altered in FZP tolerance and dependence.

关键词： benzodiazepines neuropeptide Y flura zepam

Synthesis of 1-aryl-3-(3,4-dihydro-2H-chromen-5-yl) ureas as TNF-αinhibitors

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Chinese Chemical Letters 2007年第8期18卷 905-908页

作者： Xin Ming Zhou Zhi Bing Zheng Hong Ying liu Wu Zhong Jun Hai Xiao li li wang Song li Institute of Pharmacology and Toxicology Academy of Military Medical Sciences Beijing 100850 China School of Pharmaceutical Engineering Shenyang Pharmaceutical University Shenyang 110016 China

A new series of compounds, 1-aryl-3-（3,4-dihydro-2H-chromen-5-yl） ureas, have been synthesized and their structures were confirmed by FAB-MS and IH NMR. The preliminary pharmacological screening showed that these co... 详细信息

关键词： p38 MAPK inhibitor l-Aryl-3-(3,4-dihydro-2H-chromen-5-yl) ureas Synthesis

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Effect of electroacupuncture for Pisa syndrome in Parkinson’s disease:A case report

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World Journal of Clinical Cases 2022年第30期10卷 11023-11030页

作者： Wei-Jing Lu Jing-Qi Fan Ming-Yue Yan Keiko Mukaeda li-Xing Zhuang li-li wang Clinical Medical College of Acupuncture Moxibustion and Rehabilitation Guangzhou University of Chinese MedicineGuangzhou 510006Guangdong ProvinceChina International College Guangzhou University of Chinese MedicineGuangzhou 510006Guangdong ProvinceChina The Parkinson's Clinic The First Affiliated Hospital of Guangzhou University of Chinese MedicineGuangzhou 510405Guangdong ProvinceChina

BACKGROUND Pisa syndrome(PS)refers to marked lateral flexion of the trunk with a Cobb angle greater than 10°,which is typically mobile and can be resolved by lying *** is one of the most common postural deformities secondary to Parkinson’s disease(PD)and can aggravate scoliosis in the advanced stages of *** SUMMARY Here,we present the case of a 53-year-old woman who presented with lateral curvature for 6 *** spine X-ray films in the correct position showed that the thoracolumbar spine was bent to the right without any rotation of the *** patient was diagnosed with Pisa *** receiving a month’s treatment with electroacupuncture,the Cobb angle decreased from 18.14°to 13.41°.CONCLUSION This case demonstrates that electroacupuncture can effectively improve Pisa syndrome secondary to PD with few side effects and a low risk of ***,early accurate diagnosis and timely intervention are meaningful for the prognosis of PS.

关键词： Pisa syndrome Scoliosis Parkinson disease Electroacupuncture Postural deformities Case report

Familial hemophagocytic lymphohistiocytosis type 2 in a female Chinese neonate:A case report and review of the literature

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World Journal of Clinical Cases 2021年第21期9卷 6056-6066页

作者： Shao-Hua Bi liang-liang Jiang li-Ying Dai li-li wang Guang-Hui liu Ru-Jeng Teng Division of Neonatology Department of PediatricsThe First Affiliated Hospital of Anhui Medical UniversityHefei 230022Anhui ProvinceChina Department of Neonatology Anhui Provincial Children's HospitalHefei 230022Anhui ProvinceChina Department of Pediatric Neurology Anhui Provincial Children's HospitalHefei 230022Anhui ProvinceChina Department of Pediatrics Medical College of WisconsinWauwatosaWI 53226United States

BACKGROUND Familial hemophagocytic lymphohistiocytosis type 2(FHL2)is a rare genetic disorder presenting with fever,hepatosplenomegaly,and pancytopenia secondary to perforin-1(PRF1)***2 has been described in Chinese but usually presents after 1 year *** describe a female Chinese neonate with FHL2 secondary to compound heterozygous PRF1 mutation with symptom onset before 1 mo *** review Chinese FHL2 patients in the literature for *** SUMMARY A 15-d-old female neonate was referred to our hospital for persistent fever and thrombocytopenia with diffuse *** was born to a G5P3 mother at 39 wk and 4 d via cesarean section secondary to breech *** resuscitation was required at *** was described to be very sleepy with poor appetite since *** developed a fever up to 39.5°C at 7 d of ***,anemia,and thrombocytopenia were detected at a local medical facility CONCLUSION A literature review identified 75 Chinese FHL2 patients,with only five presenting in the first year of *** and frameshift mutations are the most common PRF1 mutations in Chinese,with 24.8%having c.1349C>T followed by 11.6%having *** c.658G>C mutation has only been reported once in the literature and our case suggests it can be pathogenic,at least in the presence of another pathogenic mutation such as c.1066C>T.

关键词： Hemophagocytic lymphohistiocytosis Familial hemophagocytic lymphohistiocytosis Perforin-1 Neonate Compound heterozygous Case report

Rh-incompatible hemolytic disease of the newborn in Hefei

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World Journal of Clinical Cases 2019年第20期7卷 3202-3207页

作者： Shao-Hua Bi liang-liang Jiang li-Ying Dai Hong Zheng Jian Zhang li-li wang Chao wang Qiao Jiang Yu liu Yong-li Zhang Juan wang Chao Zhu Guang-Hui liu Ru-Jeng Teng Divisions of Neonatology Anhui Provincial Children’s HospitalHefei 230022Anhui ProvinceChina Pediatrics Neurology Anhui Provincial Children’s HospitalHefei 230022Anhui ProvinceChina Division of Neonatology First Affiliated Hospital of Anhui Medical UniversityHefei 230022Anhui ProvinceChina Hefei Blood Center Hefei 230022Anhui ProvinceChina Clinical pathology Anhui Provincial Children’s HospitalHefei 230022Anhui ProvinceChina Division of Neonatology Department of PediatricsMedical College of WisconsinMilwaukeeWI 53226United States

BACKGROUND Anti-D antibody is not the common cause of Rh-isoimmunization in Chinese neonatal *** change in national population policy has followed by an increase in Rh-isoimmunization related hemolytic disease of the newborn(HDN).Unfortunately,regional status of Rh-HDN is *** hypothesize that Rh-HDN in our region is most commonly due to anti-E *** To investigate the prevalence of hemolytic disease of the newborn due to Rhisoimmunization in Hefei *** Retrospective review of data obtained from Children’s Hospital of Anhui and Hefei Blood Center between January 2017 and June *** of minor blood group antibody was studied in the corresponding *** Totally 4138 newborns with HDN admitted during the study period and 116(2.8%)received blood exchange transfusion(BET).Eighteen newborns(0.43%)with proven Rh-incompatible HDN were *** were not the first-born *** mothers were RhD(+)(72%)and five were RhD(-).The distribution of Rh-related antibodies in mothers was ten anti-E(55%),five anti-D(27%),and for one anti-C,anti-c,and anti-E/c(6%)***(72.2%)were qualified for BET,relative risk for BET was 28.9 as compared to other types of HDN,but only 10 received due to parenteral ***(100%)RhD related HDN received BET which is not significantly different from RhE related HDN(81.8%).CONCLUSION As expected,all Rh-incompatible HDN newborns were not the *** to the Caucasian population,anti-D induced HDN is not the most common *** our region,anti-E(11/18,61%)is the most common cause of Rh-HDN.

关键词： Rh-isoimmunization Hemolytic disease of the newborn Minor blood group

Krüppel-like factor 8 overexpression is correlated with angiogenesis and poor prognosis in gastric cancer

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World Journal of Gastroenterology 2013年第27期19卷 4309-4315页

作者： Wen-Fei wang Juan li Lu-Tao Du li-li wang Yong-Mei Yang Yi-Min liu Hui liu Xin Zhang Zhao-Gang Dong Gui-Xi Zheng ChuanXin wang School of Biosciences University of NottinghamSutton Bonington Campus Department of Clinical Laboratory Qilu HospitalShandong University

AIM:To investigate Krüppel-like factor 8 (KLF8) expression in gastric cancer and its relationship with angiogenesis and prognosis of gastric cancer. METHODS:One hundred and fifty-four patients with gastric cancer who underwent successful curative resection were retrospectively enrolled in the study. Fifty tumor-adjacent healthy gastric tissues (≥ 5 cm from the tumor margin) obtained during the original resection were randomly selected for comparative analysis. In situ expression of KLF8 and CD34 proteins were examined by immunohistochemistry. The intratumoral microvessel density (MVD) was determined by manually counting the immunostained CD34-positive endothelial cells in three consecutive high-magnification fields (× 200). The relationship between differential KLF8 expression and MVD was assessed using Spearman's correlation coefficient test. χ2 test was performed to evaluate the effects of differential KLF8 expression on clinicopathologic factors. Kaplan-Meier and multivariate Cox survival analyses were used to assess the prognostic value of differential KLF8 expression in gastric cancer. RESULTS:Significantly higher levels of KLF8 protein were detected in gastric cancer tissues than in the adjacent non-cancerous tissues (54.5% vs 34.0%, P < 0.05). KLF8 expression was associated with tumor size (P < 0.001), local invasion (P = 0.005), regional lymph node metastasis (P = 0.029), distant metastasis (P = 0.023), and tumor node metastasis (TNM) stage (P = 0.002), as well as the MVD (r = 0.392, P < 0.001). Patients with KLF8 positive expression had poorer overall survival (P < 0.001) and cancer-specific survival (P < 0.001) than those with negative expression. Multivariate analysis demonstrated that KLF8 expression independently affected both overall and cancer-specific survival of gastric cancer patients (P = 0.035 and 0.042, respectively). CONCLUSION:KLF8 is closely associated with gastric tumor progression, angiogenesis and poor prognosis, suggesting it may represen

关键词： Gastric cancer Krüppel-like factor 8 Angiogenesis Prognosis

Long noncoding RNA RP4 functions as a competing endogenous RNA through miR-7-5p sponge activity in colorectal cancer

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World Journal of Gastroenterology 2018年第9期24卷 1004-1012页

作者： Mu-lin liu Qiao Zhang Xiao Yuan Long Jin li-li wang Tao-Tao Fang Wen-Bin wang Department of Gastrointestinal Surgery the First Affiliated Hospital of Bengbu Medical College Department of General Surgery the First Affiliated Hospital of Xinxiang Medical University Department of General Surgery the Fourth Affiliated Hospital of Anhui Medical University

AIM To investigate the role of long noncoding RNA(lnc RNA) RP4 in colorectal *** Lentivirus-mediated lnc RNA RP4 overexpression and knockdown were performed in the colorectal cancer cell line SW480. Cell proliferation, tumor growth, and early apoptosis were evaluated by a cell counting kit-8 assay, an in vivo xenograft tumor model, and annexin V/propidium iodide staining, respectively. Analysis of the lnc RNA RP4 mechanism involved assessment of the association of its expression with mi R-7-5 p and the SH3 GLB1 gene. Western blot analysis was also performed to assess the effect of lnc RNA RP4 on the autophagy-mediated cell death pathway and phosphatidylinositol-3-kinase(PI3 K)/Akt *** Cell proliferation, tumor growth, and early apoptosis in SW480 cells were negatively regulated by lnc RNA RP4. Functional experiments indicated that lnc RNA RP4 directly upregulated SH3 GLB1 expression by acting as a competing endogenous RNA(ce RNA) for mi R-7-5 p. This interaction led to activation of the autophagy-mediated cell death pathway and de-repression of PI3 K and Akt phosphorylation in colorectal cancer cells in *** Our results demonstrated that lnc RNA RP4 is a ce RNA that plays an important role in the pathogenesis of colorectal cancer, and could be a potential therapeutic target for colorectal cancer treatment.

关键词： Colorectal cancer long noncoding RNA RP4 SH3GLB1 miR-7-5p competing endogenous RNA

Study on the α-cyclodextrin/poly(ethylene glycol) self-assembly supramolecular nanoparticles for drug delivery

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Science China Chemistry 2010年第3期53卷 495-501页

作者： li Yuan,JI li,wang Gang,SONG liQing,HE Bin,li li,NIE Yu,WU Yao & GU ZhongWei National Engineering Research Center for Biomaterials,Sichuan University,Chengdu 610064,China National Engineering Research Center for Biomaterials Sichuan University Chengdu China

This paper reports the synthesis and drug delivery properties of a novel supramolecular nanoparticle.α-Cyclodextrins(α-CD) were threaded on cinnamic acid modified poly(ethylene glycol) to form inclusion complex nanoparticles by supramolecular *** anti-tumor drug doxorubicin was loaded in the nanoparticles and released in vitro to study the drug release behavior and the anti-tumor *** structure and morphology of the nanoparticles were characterized by nuclear magnetic resonance,X-ray diffraction,ultraviolet absorbance,dynamic laser scattering,scanning electronic microscopy,transmission electron microscopy and atom force *** distribution of the drug loaded nanoparticles in cells and the anti-tumor effects were studied by confocal laser *** results demonstrate that the supramolecular nanoparticle is biocompatible and it is a promising carrier for drug delivery systems.

关键词： supramolecular self-assembly nanoparticles drug delivery

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World Journal of Gastroenterology 2012年第45期18卷 6571-6576页

作者： Quan-Jiang Dong Shu-Hui Zhan li-li wang Yong-Ning Xin Man Jiang Shi-Ying Xuan Central Laboratories Department of GastroenterologyQingdao Municipal Hospital

Helicobacter pylori （H. pylorl） is a Gram-negative bac- terium that infects half of the human population. The infection is associated with chronic inflammation of the gastric mucosa and peptic ulcers. It is also a major risk factor for gastric cancer. Phylogenetic analysis of global strains reveals there are seven populations of H. pylori, including hpAfrical, hpAfrica2, hpEastAsia, hpEurope, hpNEAfrica, hpAsia2 and hpSahul. These populations are consistent with their geographical origins, and pos- sibly result from geographical separation of the bac- terium leading to reduced bacterial recombination in some populations. For each population, H. pylori has evolved to possess genomic contents distinguishable from others. The hpEurope population is distinct in that it has the largest genome of 1.65 mbp on average, and the highest number of coding sequences. This confers its competitive advantage over other populations but at the cost of a lower infection rate. The large genomic size could be a cause of the frequent occurrence of the deletion of the cag pathogenicity island in H. pylori strains from hpEurope. The incidence of gastric cancer varies among different geographical regions. This can be attributed in part to different rates of infection of H. pylori. Recent studies found that different popula- tions of H, pylori vary in their carcinogenic potential and contribute to the variation in incidence of gastric cancer among geographical regions. This could be related to the ancestral origin of H, pylori. Further studies are indi- cated to investigate the bacterial factors contributing to differential virulence and their influence on the clinical features in infected individuals.

关键词： Helicobacter pylori Population genetics Gastric cancer Virulence Genome