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Ratiometric fluorescence probe for accurate detection of Concanavalin A by coupling fluorescent microsphere with boric acid functionalized carbon dots

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Chinese Chemical Letters 2024年第2期35卷 460-463页

作者： Mingyue Xie juan Chen Yufei Wang Bojun liu Rong-Bin song Hong-Min Meng Zhaohui li College of Chemistry Institute of Analytical Chemistry for Life ScienceHenan Joint International Research Laboratory of Green Construction of Functional Molecules and their Bioanalytical ApplicationsZhengzhou UniversityZhengzhou 450001China Zhengzhou Key Laboratory of Forensic Science and Technology Railway Police CollegeZhengzhou 450053China School of Ecology and Environment Zhengzhou UniversityZhengzhou 450001China

Accurate and sensitive strategies for Concanavalin A(Con A)sensing are conducive to the better cognition of various important biological and physiological processes.Here,by designing dextran-functionalized fluorescent microspheres(DxFMs)and boric acid-modified carbon dots(BCDs)as recognition unit and built-in signal reference respectively,a ratiometric fluorescent detection platform was proposed for Con A detection with high reliability.In this protocol,the BCDs/DxFMs precipitation was formed due to the covalent interactions between cis-diol of DxFMs and boronic acid groups of BCDs,thus only fluorescence of BCDs could be detected in the supernatant.When Con A was presented,it could bind to DxFMs through its carbohydrate recognition ability and suppress the subsequent assembly between DxFMs and BCDs,leading to the simultaneous capture of DxFMs and BCDs fluorescence in the supernatant.Since the BCDs content was superfluous,their fluorescence intensities were basically constant in all cases.Based on the unchanged BCDs fluorescence signal and target-dependent DxFMs fluorescence signal in supernatant,the ratiometric detection of Con A was realized.Under optimized conditions,this ratiometric fluorescent platform displayed a linear detection range from 0.125μg/mL to 12.5μg/mL with a detection limit of 0.089μg/mL.Moreover,satisfied analytical outcomes for Con A detection in serum samples were obtained,manifesting huge application potential of this ratiometric fluorescent platform in clinical diagnosis.

关键词： Concanavalin A Fluorescent microspheres Carbon dots Ratiometric fluorescent assay Reliability

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E2E-MFERC:AMulti-Face Expression Recognition Model for Group Emotion Assessment

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Computers, Materials & Continua 2024年第4期79卷 1105-1135页

作者： lin Wang juan Zhao Hu song Xiaolong Xu Jiangsu Key Laboratory of Big Data Security&Intelligent Processing Nanjing University of Posts and TelecommunicationsNanjing210042China School of Network Security Jinling Institute of TechnologyNanjing211169China State Grid Jiangsu Electric Power Company limited Nanjing210000China School of Computer Science Nanjing University of Posts and TelecommunicationsNanjing210042China

In smart classrooms, conducting multi-face expression recognition based on existing hardware devices to assessstudents’ group emotions can provide educators with more comprehensive and intuitive classroom effect analysis,thereby continuouslypromotingthe improvementof teaching quality.However,most existingmulti-face expressionrecognition methods adopt a multi-stage approach, with an overall complex process, poor real-time performance,and insufficient generalization ability. In addition, the existing facial expression datasets are mostly single faceimages, which are of low quality and lack specificity, also restricting the development of this research. This paperaims to propose an end-to-end high-performance multi-face expression recognition algorithm model suitable forsmart classrooms, construct a high-quality multi-face expression dataset to support algorithm research, and applythe model to group emotion assessment to expand its application value. To this end, we propose an end-to-endmulti-face expression recognition algorithm model for smart classrooms (E2E-MFERC). In order to provide highqualityand highly targeted data support for model research, we constructed a multi-face expression dataset inreal classrooms (MFED), containing 2,385 images and a total of 18,712 expression labels, collected from smartclassrooms. In constructing E2E-MFERC, by introducing Re-parameterization visual geometry group (RepVGG)block and symmetric positive definite convolution (SPD-Conv) modules to enhance representational capability;combined with the cross stage partial network fusion module optimized by attention mechanism (C2f_Attention),it strengthens the ability to extract key information;adopts asymptotic feature pyramid network (AFPN) featurefusion tailored to classroomscenes and optimizes the head prediction output size;achieves high-performance endto-end multi-face expression detection. Finally, we apply the model to smart classroom group emotion assessmentand provide design references for classroom effect analysis evaluation

关键词： Multi-face expression recognition smart classroom end-to-end detection group emotion assessment

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Trial application of the envelope method to the potential ambiguity problem

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Nuclear Science and Techniques 2024年第1期35卷 73-80页

作者： li-Yuan Hu Yu-Shou song Fundamental Science on Nuclear Safety and Simulation Technology Laboratory Harbin Engineering UniversityHarbin 150001China College of Nuclear Science and Technology Harbin Engineering UniversityHarbin 150001China Key Laboratory of Nuclear Safety and Advanced Nuclear Energy Technology Ministry of Industry and Information TechnologyHarbin 150001China

The optical potential ambiguity is a long-standing problem in the analysis of elastic scattering data.For a specific collid-ing system,ambiguous potential families can lead to different behaviors in the nearside and farside scattering components.By contrast,the envelope method can decompose the experimental data into two components with negative and positive deflection angles,respectively.Hence,a question arises as to whether the comparison between the calculated nearside(or farside)component and the derived positive-deflection-angle(or negative-deflection-angle)component can help analyze the potential ambiguity problem.In this study,we conducted a trial application of the envelope method to the potential ambiguity problem.The envelope method was improved by including uncertainties in the experimental data.The colliding systems of 16O+28Si at 215.2 MeV and 12C+12C at 1016 MeV were considered in the analyses.For each colliding system,the angular distribution experimental data were described nearly equally well by two potential sets,one of which is“surface transpar-ent”and the other is refractive.The calculated angular distributions were decomposed into nearside and farside scattering components.Using the improved envelope method,the experimental data were decomposed into the positive-deflection-angle and negative-deflection-angle components,which were then compared with the calculated nearside and farside components.The capability of the envelope method to analyze the potential ambiguities was also discussed.

关键词： Elastic scattering Optical potential ambiguities Envelope method Nearside/farside decomposition

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Functional Optical Fiber Sensors Detecting Imperceptible Physical/Chemical Changes for Smart Batteries

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Nano-Micro Letters 2024年第8期16卷 268-308页

作者： Yiding li li Wang Youzhi song Wenwei Wang Cheng lin Xiangming He Institute of Nuclear and New Energy Technology Tsinghua UniversityBeijing 100084People’s Republic of China National Engineering Research Center of Electric Vehicles Beijing Institute of Technology(BIT)Beijing 100081People’s Republic of China Shenzhen Automotive Research Institute of BIT(Shenzhen Research Institute of National Engineering Research Center of Electric Vehicles) Shenzhen 518118People’s Republic of China

The battery technology progress has been a contradictory process in which performance improvement and hidden risks coexist.Now the battery is still a“black box”,thus requiring a deep understanding of its internal state.The battery should“sense its internal physical/chemical conditions”,which puts strict requirements on embedded sensing parts.This paper summarizes the application of advanced optical fiber sensors in lithium-ion batteries and energy storage technologies that may be mass deployed,focuses on the insights of advanced optical fiber sensors into the processes of one-dimensional nano-micro-level battery material structural phase transition,electrolyte degradation,electrode-electrolyte interface dynamics to three-dimensional macro-safety evolution.The paper contributes to understanding how to use optical fiber sensors to achieve“real”and“embedded”monitoring.Through the inherent advantages of the advanced optical fiber sensor,it helps clarify the battery internal state and reaction mechanism,aiding in the establishment of more detailed models.These advancements can promote the development of smart batteries,with significant importance lying in essentially promoting the improvement of system consistency.Furthermore,with the help of smart batteries in the future,the importance of consistency can be weakened or even eliminated.The application of advanced optical fiber sensors helps comprehensively improve the battery quality,reliability,and life.

关键词： Smart battery Advanced embedded optical fiber sensor Battery internal physical/chemical state Quality-reliability-life characteristic

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Experimental study on combustion characteristics of supersonic combustor based on alternating-wedge strut

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Chinese Journal of Aeronautics 2024年第5期37卷 214-227页

作者： Jie li Wenyan song Yuhang WANG School of Power Energy Northwestern Polytechnical UniversityXi’an 710129China

Acetone Planar Lase-Induced Fluorescence(PliF)and OH-PliF were employed to capture the fuel distribution and OH distribution downstream for the supersonic combustor based on the alternating-wedge strut.The combustion establishment process and combustion mode in the combustor under different fuel injection methods and different equivalence ratios were analyzed.Combined with the kerosene-PliF and OH-PliF results in the cavity combustor,a comparative analysis was conducted to understand the combustion characteristics and combustion modes between the alternating-wedge strut-based combustor and the cavity-based combustor.The results show that the combustor is in weak combustion mode in the case of low equivalence ratio,and the combustor is in intensive combustion mode in the case of high equivalence ratio.The lower limit of the equivalence ratio of the combustor to maintain the intensive combustion mode varies based on different fuel injection methods.The OH distribution under reacting condition has a strong correlation with the fuel distribution under non-reacting condition.The OH fluorescence signal near the injector is weaker when the fuel distribution is more concentrated.The injector position located at the base of the strut rear has better mixing performance,enabling the combustor to be in intensive combustion mode at a lower equivalence ratio.The combustion reaction in the alternating-wedge strut-based combustor is not necessarily dominated by mass transfer due to the mixing enhancement and premixed zone downstream of strut,while the combustion reaction process in the cavity-based combustor is mainly influenced by mass transfer.

关键词： Supersonic combustor Alternating-wedge strut Cavity PliF Fuel injection Fuel distribution Combustion characteristics Combustion mode

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Adaptive Consensus of Uncertain Multi-Agent Systems With Unified Prescribed Performance

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IEEE/CAA Journal of Automatica Sinica 2024年第5期11卷 1310-1312页

作者： Kun li Kai Zhao Yongduan song The authors are with the School of Automation Chongqing UniversityChongqing 400044China

Dear Editor,An adaptive consensus control algorithm for uncertain multi-agent systems(MAS),capable of guaranteeing unified prescribed performance,is presented in this letter.Unlike many existing prescribed performance related works,the developed control exhibits some features.Firstly,a distributed prescribed time observer is introduced so that not only each follower is able to estimate the leader’s signal within a predetermined time,but also the control design for each agent is independent with its neighbors.

关键词： agent prescribed Uncertain

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Enhanced Electrical Properties of Styrene-grafted Polypropylene Insulation for Bulk Power Transmission HVDC Cables

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CSEE Journal of Power and Energy Systems 2024年第1期10卷 361-370页

作者： Hao Yuan Shixun Hu Yao Zhou Chao Yuan Wenbo song Qing Shao Hongwei Shi juan li Jun Hu Qi li Jinliang He SINOPEC Beijing Research Institute of Chemical Industry Beijing 100013China State Key Laboratory of Power Systems Department of Electrical EngineeringTsinghua UniversityBeijing 100084China

Polypropylene(PP)is considered to be a rather promising material for HVDC cable insulation.Grafting modification has proved to be an effective method on the electrical property improvements of PP.This paper reports on the highlyenhanced electrical properties of styrene-grafted polypropylene(PP-g-St)and their related mechanism.The influence of grafting styrene on crystallization structures,thermal properties and electrical properties of PP-g-St are characterized and analyzed.These results show that PP-g-St exhibits remarkably enhanced DC volume resistivity,suppressed space charge accumulation and improved DC breakdown strengths as compared to PP.Crystallization observation shows PP-g-St has small,imperfect spherulites with indistinct boundaries,which is strongly correlated to enhanced electrical properties.This study provides an effective grafting strategy to design high-performance HVDC cable insulation materials for bulk power transmission systems.

关键词： Electrical properties grafting modification polypropylene styrene

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Tetrabromobisphenol A exerts thyroid disrupting effects but has little overt impact on postnatal brain development and neurobehaviors in mice

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Journal of Environmental Sciences 2024年第8期142卷 1-10页

作者： Shilin song Yuanyuan li lin Lv Mengqi Dong Zhanfen Qin State Key Laboratory of Environmental Chemistry and Ecotoxicology Research Center for Eco-Environmental SciencesChinese Academy of SciencesBeijing 100085China College of Resources and Environment University of Chinese Academy of SciencesBeijing 100049China School of Environment Tsinghua UniversityBeijing 100084China

Tetrabromobisphenol A(TBBPA)is a widely used brominated flame retardant.There is evidence showing that TBBPA can exert thyroid disrupting effects in mammals,but different results were also reported,along with inconsistent reports regarding its neurotoxicity.Here,we investigated thyroid disrupting effects and neurotoxicity of TBBPA(5,50,500μg/(kg·day))to male mice following maternal and direct exposure through drinking water,with the antithyroid drug propylthiouracil(PTU)as the positive control.On postnatal day(PND)15,we expectedly observed severe thyroid compensatory hyperplasia and cerebellar developmental retardation in PTU-treated pups.The highest dose of TBBPA also caused thyroid histological alteration but had no effects on cerebellar development in terms of Purkinje cell morphology and the thickness of the internal granular layer and the molecular layer of the cerebellum.During puberty and adulthood,the thyroid morphological alterations became more pronounced in the TBBPA-treated animals,accompanied by decreased serum thyroid hormone levels.Furthermore,the 50 and 500μg/(kg·day)TBBPA groups showed a significant decrease in the serum level of serotonin,a neurotransmitter associated with anxiety behaviors.Correspondingly,the highest dose group displayed anxiety-like behaviors in the elevated plus-maze test on PND 35,but this neurobehavioral alteration disappeared on PND 56.Moreover,no changes in neurobehavioral parameters tested were found in TBBPAtreated animals at puberty and adulthood.Altogether,all observations show that TBBPA can exert thyroid disrupting effects but has little overt impact on brain development and neurobehaviors in mice,suggesting that thyroid disruption does not necessarily cause overtly adverse neurodevelopmental outcomes.

关键词： Tetrabromobisphenol A Thyroid disruption Brain development Neurobehavior Cerebellum

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Earthquake potential across the North–South seismic belt of China

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Science Bulletin 2024年第2期69卷 179-182页

作者： Yanchuan li Xinjian Shan Chunyan Qu Guohong Zhang Xiaogang song State Key Laboratory of Earthquake Dynamics Institute of GeologyChina Earthquake AdministrationBeijing 100029China

The elastic rebound theory,which frames our understanding of earthquakes,suggests that the accumulated elastic strain on faults will ultimately be released through earthquakes[1].As such,identifying the location,rate,and accumulation of slip deficit on faults constitutes a basis for seismic potential assessment[2].Interseismic coupling(ISC)refers to the ratio of the slip deficit rate on a fault to the far-field loading rate,where full and no coupling correspond to 100%and 0%slip deficits,respectively.

关键词： seismic belt fault

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Fully distributed dynamic event-triggered formation-containment tracking for multiagent systems with multiple types of disturbances

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Science China(Information Sciences) 2024年第1期67卷 168-186页

作者： Weihua li Huaguang ZHANG juan ZHANG Rui WANG College of Information Science and Engineering Northeastern University State Key Laboratory of Synthetical Automation for Process Industries Northeastern University

This paper is dedicated to addressing the time-varying output formation-containment tracking(TVOFCT) problem for heterogeneous linear multiagent systems(MASs) with multiple types of disturbances under directed communication topology.The agents are divided into tracking leaders,formation leaders,and followers.In the output space,the formation leaders are required to form an expected geometric formation while tracking the tracking leader,and the followers are required to enter the convex hull spanned by formation leaders.First,a disturbance observer is designed to observe the disturbance with deterministic dynamics.Then,by combining adaptive technology with event-triggered technology,an independent fully distributed dynamic event-triggered(FDDET) compensator is designed to estimate the state of the tracking leader.The main advantages of this compensator lie in the following three aspects:(1) the adaptive weight does not increase unboundedly;(2) the interval time between events is expanded;(3) the design does not rely on any global information.Similar to the design of this compensator,an independent FDDET convex hull observer is further designed to observe the convex hull spanned by formation leaders.Subsequently,based on the designed disturbance observer,compensator,and convex hull observer,the adaptive disturbance rejection control input which can make the MASs achieve TVOFCT is formulated.Finally,a numerical simulation is provided to clearly verify the validity of the theoretical results.

关键词： formation-containment tracking disturbance rejection multiagent systems fully distributed control dynamic event-triggered control

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