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Plant Mediator complex and its critical functions in transcription regulation

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Journal of Integrative Plant Biology 2016年第2期58卷 106-118页

作者： Yan Yang ling li li-jia qu State Key Laboratory of Protein and Plant Gene Research Peking-Tsinghua Center for Life Sciences College of Life Sciences Peking University Beijing 100871 China The National Plant Gene Research Center (Beijing) Beijing 100101 China.

The Mediator complex is an important component of the eukaryotic transcriptional machinery. As an essential link between transcription factors and RNA polymerase II, the Mediator complex transduces diverse signals to genes involved in different pathways. The plant Mediator complex was recently purified and comprises conserved and specific subunits. It functions in concert with transcription factors to modulate various responses. In this review, we summarize the recent advances in understanding the plant Mediator complex and its diverse roles in plant growth, development, defense, non-coding RNA production, response to abiotic stresses, flowering, genomic stability and metabolic homeostasis. In addition, the transcription factors interacting with the Mediator complex are also highlighted.

关键词： Mediator complex transcription factors transcription regulation

Cysteine-rich peptides: signals for pollen tube guidance, species isolation and beyond

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Science China(life Sciences) 2019年第9期62卷 1243-1245页

作者： Sheng Zhong li-jia qu State Key Laboratory for Protein and Plant Gene Research Peking-Tsinghua Center for Life Sciences at College of Life SciencesPeking UniversityBeijing 100871China The National Plant Gene Research Center (Beijing) Beijing 100101China

In flowering plants,the two sperm cells are passive cargo transported into the ovule by pollen tubes (Zhang et al.,2017).Therefore,the precise guidance of pollen tubes is critical for successful double fertilization (Dresselhaus and Franklin-Tong,2013).A series of male-female interactions are required to guarantee the spatiotemporal regulation of pollen tube guidance because during growth,pollen tubes continuously interact with different female tissues (Zhong et al.,2017;Zhong and qu,2019).In the past two decades,there has been tremendous progress in elucidating the molecular mechanisms of pollen tube guidance regulation,mostly using Arabidopsis thaliana as a model system (Johnson et al.,2019).

关键词： double fertilization pollen tube model system

Response to Zhang et al.,‘do egg cell-secreted aspartic proteases promote gamete attachment?'

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Journal of Integrative Plant Biology 2023年第1期65卷 7-9页

作者： jiahao jiang li-jia qu State Key Laboratory for Protein and Plant Gene Research Peking-Tsinghua Center for Life Sciences at College of Life Sciences Peking UniversityBeijing 100871 China

In angiosperms,the molecular mechanism controlling polytubey/polyspermy block and fertilization recovery was not unraveled until recently(Zhong et al.,2022),which can be described as fol ows:Five pol en tube-expressed RALF peptide ligands,perceived by the receptor complex composed of three receptors FER/ANJ/HERK1 at the septum,establish the polytubey block at the septum so that only one pollen tube is allowed to emerge from the septum to target the ovule for *** tube rupture releases the two sperm cells and fertilization is completed within 20 min.

关键词： allowed release controlling

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Journal of Integrative Plant Biology 2012年第10期54卷 814-827页

作者： Guo Luo Hongya Gu Jingjing liu li-jia qu State Key Laboratory for Protein and Plant Gene Research College of Life SciencesPeking-Tsinghua Center of Life SciencesPeking UniversityBeijing 100871China The National Plant Gene Research Center Beijing 100101China

Ubiquitination of proteins is one of the critical regulatory mechanisms in eukaryotes. In higher plants, protein ubiquitination plays an essential role in many biological processes, including hormone signaling, photomorphogenesis, and pathogen defense. However, the roles of protein ubiquitination in the repro- ductive process are not clear. In this study, we identified four plant-specific RING-finger genes designated Aberrant_Pollen Development 1_ （APD1） to APD4, as regulators of pollen mitosis II （PMII） in Arabidopsis thaliana （L.）. The apdl apd2 double mutant showed a significantly increased percentage of bicellular-like pollen at the mature pollen stage. Further downregulation of the APD3 and APD4 transcripts in apdl apd2 by RNA interference （RNAi） resulted in more severe abnormal bicellular-like pollen phenotypes than in apdl apd2, suggesting that cell division was defective in male gametogenesis. All of the four genes were expressed in multiple stages at different levels during male gametophyte development. Confocal analysis using green florescence fusion proteins （GFP） GFP-APD1 and GFP-APD2 showed that APDs are associated with intracellular membranes. Furthermore, APD2 had E2-dependent E3 ligase activity in vitro, and five APD2-interacting proteins were identified. Our results suggest that these four genes may be involved, redundantly, in regulating the PMII process during male gametogenesis.

关键词： Male gametogenesis pollen mitosis II RING-finger E3 ligase Arabidopsis.

Plant reproduction: Recent discoveries from China

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Journal of Integrative Plant Biology 2017年第9期59卷 591-593页

作者： li-jia qu Meng-Xiang Sun College of life Sciences Peking UniversityBeijingChina College of life Sciences Wuhan UniversityWuhanChina

Sexual reproduction in higher plants consists of several major biological processes which include mega- and micro-gametogenesis, male-female communications, embryogenesis and endosperm development. Male-female communications include male gametophyte-sporophytic tissues （stigma/transmitting tract） interaction and male-female gametophyte communication, during which various signaling pathways are involved.

关键词： Recent discoveries from China

Characterization of Arabidopsis MYB transcription factor gene AtMYB17 and its possible regulation by LEAFY and AGL15

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Journal of Genetics and Genomics 2009年第2期36卷 99-107页

作者： Yunfei Zhang Guangyu Cao li-jia qu Hongya Gu National Laboratory of Protein Engineering and Plant Genetic Engineering College of Life Sciences Peking University Beijing 100871 China

MYB transcription factors compose one of the largest transcription factor families in Arabidopsis, which play important roles in vari- ous developmental processes as well as defense responses against environmental stresses. In this study, we report the characterization of AtMYB17 gene, a putative R2R3 type MYB gene family member in Arabidopsis. AtMYB17 was found exclusively localized in nuclear, with an activation domain at its C-terminus. AtMYBI7 was highly expressed in inflorescences and siliques, especially at early flower developmental stages. The level of AtMYB17 transcripts was also found to increase after imbibition during seed germination and gradually concentrate to the shoot apex. Bioinformatics analysis identified several binding sites of LEAFY （LFY） and AGL15 in the promoter re- gion of AtMYB17. Promoter-GUS fusion analysis showed that the LFY binding sites were important in fine-tuning regulation of the spatio-temporal expression ofAtMYB17 in transgenic plants. Moreover, AtMYB17 was up-regulated in 35S：：AGL15 plants. Taken together, our data suggest that LFY may be involved in the regulation of AtMYB17, possibly together with AGL 15, and thereafter in early inflores- cence development and seed germination.

关键词： AtMYB17 transcription factor Arabidopsis LEAFY AGL 15

Insights into pollen-stigma recognition:self-incompatibility mechanisms serve as interspecies barriers in Brassicaceae?

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aBIOTECH 2023年第2期4卷 176-179页

作者： Zijun Lan Sheng Zhong li-jia qu State Key Laboratory for Protein and Plant Gene Research Peking-Tsinghua Center for Life SciencesNew Cornerstone Science LaboratoryCollege of Life SciencesPeking UniversityBeijing100871China

A new study provides a comprehensive molecular mechanism that controls interspecific incompatibility of self-incompatible(SI)plants in the *** finding points to a potentially promising path to break interspecific barriers and achieve introgression of desirable traits into crops from distant species among SI crops in the Brassicaceae.

关键词： Pollen-stigma recognition Self-incompatibility Interspecific barriers ROS

The signals to trigger the initiation of ovule enlargement are from the pollen tubes: The direct evidence

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Journal of Integrative Plant Biology 2017年第9期59卷 600-603页

作者： Sheng Zhong Jun Zhang li-jia qu State Key Laboratory for Protein and Plant Gene Research Peking-Tsinghua Center for Life Sciences at College of Life SciencesPeking UniversityBeijing 100871China The National Plant Gene Research Center(Beijing) Beijing 100101China

In angiosperms, initiation of ovule enlargement represents the start of seed development, the molecular mechanism of which is not yet elucidated. It was previously reported that pollen tube contents, rather than double fertilization, can trigger ovule enlargement. However, it remains unclear whether the signal（s） to trigger the initiation of ovule enlargement are from the sperm cells or fromthe pollen tubes. Recently, we identified a mutant dropl- drop2-, which produces pollen tubes with no sperm cells. Taking advantage of this special genetic material, we conducted pollination assays, and found that the ovules pollinated with dropl- drop2- pollen could initiate the enlargement and exhibited significant enlarged sizes at 36h after pollination in comparison with those unpollinated ovules. However, the sizes of the ovules pollinated with drops- drop2- pollen are significantly smaller than those of the ovules pollinated with wildtype pollen. These results demonstrate that the pollen tube, rather than the sperm cells, release the signal to trigger the initiation of ovule enlargement, and that double fertilization is required for further enlargement of the seeds.

关键词： The signals to trigger the initiation of ovule enlargement are from the pollen tubes The direct evidence Figure

Hormonal Regulation of Leaf Morphogenesis in Arabidopsis

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Journal of Integrative Plant Biology 2007年第1期49卷 75-80页

作者： lin-Chuan li Ding-Ming Kang Zhang-liang Chen li-jia qu National Laboratory for Protein Engineering and Plant Genetic Engineering Peking-Yale Joint Research Center for Plant Molecular Genetics and AgroBiotechnology College of Life Sciences Peking University Beijing 100871 China Key Laboratory of Crop Genomics and Genetic Improvement of Ministry of Agriculture/Key laboratory of Crop Genetic Improvement of Beijing City College of Agronomy and Biotechnology China Agricultural University Beijing 100094 China

Leaf morphogenesis is strictly controlled not only by intrinsic genetic factors, such as transcriptional factors, but also by environmental cues, such as light, water and pathogens. Nevertheless, the molecular mechanism of how leaf morphogenesis is regulated by genetic programs and environmental cues is far from clear. Numerous series of events demonstrate that plant hormones, mostly small and simple molecules, play crucial roles in plant growth and development, and in responses of plants to environmental cues such as light. With more and more genetics and molecular evidence obtained from the model plant Arabidopsis, several fundamental aspects of leaf morphogenesis including the initiation of leaf primordia, the determination of leaf axes, the regulation of cell division and expansion in leaves have been gradually unveiled. Among these phytohormones, auxin is found to be essential in the regulation of leaf morphogenesis.

关键词： auxin curly leaf hormonal regulation leaf morphogenesis leaf shape