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Neural Regeneration Research 2016年第5期11卷 801-806页

作者： Wei li li-hong kong Hui Wang Feng Shen Ya-wen Wang Hua Zhou Guo-jie Sun Hubei University of Chinese Medicine Wuhan Hubei Province China School of Acupuncture Orthopedics Hubei University of Chinese Medicine Wuhan Hubei Province China

The frequency range of electroacupuncture in treatment of Alzheimer＇s disease in rats is commonly 2-5 Hz（low frequency） and 50-100 Hz（high frequency）. We established a rat model of Alzheimer＇s disease by injecting β-amyloid 1-42（Aβ1-42） into the bilateral hippocampal dentate gyrus to verify which frequency may be better suited in treatment. Electroacupuncture at 2 Hz or 50 Hz was used to stimulate Baihui（DU20） and Shenshu（BL23） acupoints. The water maze test and electrophysiological studies demonstrated that spatial memory ability was apparently improved, and the ranges of long-term potentiation and long-term depression were increased in Alzheimer＇s disease rats after electroacupuncture treatment. Moreover, the effects of electroacupuncture at 50 Hz were better than that at 2 Hz. These findings suggest that high-frequency electroacupuncture may enhance hippocampal synaptic transmission and potentially improve memory disorders in Alzheimer＇s disease rats.

关键词： nerve regeneration Alzheimer's disease frequency electroacupuncture long-term potentiation long-term depression learning and memory Baihui (DU20) Shenshu (BL23) neural regeneration

Identification and Characterization of 43 Microsatellite Markers Derived from Expressed Sequence Tags of the Sea Cucumber (Apostichopus japonicus)

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Journal of Ocean University of China 2011年第2期10卷 185-190页

作者： JIANG Qun li Qi YU hong kong lingfeng Fisheries College Ocean University of China Qingdao 266003 China

The sea cucumber Apostichopus japonicus is a commercially and ecologically important species in China. A total of 3056 potential unigenes were generated after assembling 7597 A. japonicus expressed sequence tags (ESTs) downloaded from Gen-Bank. Two hundred and fifty microsatellite-containing ESTs (8.18%) and 299 simple sequence repeats (SSRs) were detected. The average density of SSRs was 1 per 7.403 kb of EST after redundancy elimination. Di-nucleotide repeat motifs appeared to be the most abundant type with a percentage of 69.90%. Of the 126 primer pairs designed, 90 amplified the expected products and 43 showed polymorphism in 30 individuals tested. The number of alleles per locus ranged from 2 to 26 with an average of 7.0 alleles, and the observed and expected heterozygosities varied from 0.067 to 1.000 and from 0.066 to 0.959, respectively. These new EST-derived microsatellite markers would provide sufficient polymorphism for population genetic studies and genome mapping of this sea cucumber species.

关键词： sea cucumber Apostichopus japonicus microsatellite EST

CONTACT DEFORMATION AND PRE-CONTROL OF TRANSMISSION PROPERTIES OF POINT CONJUGATE GEAR

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Chinese Journal of Mechanical Engineering 2000年第1期13卷 76-80页

作者： liu Huran Yuan Wenhui (Department of Mechanical Engineering, Changsha Railway Institute) li Rongbin (The hong kong Polytechnic University) 长沙铁道学院湖南长沙香港理工大学香港

According to spatial conjugate principle and theory of elastic contact, a method to pre-control transmission properties and contact mark of point meshing gear is presented, while the deformation of tooth surface is un... 详细信息

关键词： Elastic Contact Deformation Meshing Gear

High-frequency (50 Hz) electroacupuncture ameliorates cognitive impairment in rats with amyloid beta 1–42-induced Alzheimer＇s disease

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Neural Regeneration Research 2018年第10期13卷 1833-1841页

作者： Chao-Chao Yu Ying Wang Feng Shen li-hong kong Ya-Wen Wang Hua Zhou Lei Tang Hubei University of Chinese Medicine WuhanHubei ProvinceChina Department of Acupuncture and Moxibustion College of Acupuncture and OrthopedicsHubei University of Chinese MedicineWuhanHubei ProvinceChina Department of Rehabilitation Wuhan Central Hospital Affiliated to Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei ProvinceChina

Acupuncture has been shown to ameliorate cognitive impairment of Alzheimer’s *** and stimulation frequency influence the therapeutic effect of *** models of Alzheimer’s disease were established by injecting amyloid beta 1–42（Aβ_（1–42））into the bilateral lateral *** at 2,30,and 50 Hz was carried out at Baihui（GV20;15°obliquely to a depth of 2mm）and Shenshu（BL23;perpendicularly to 4–6 mm depth）,once a day for 20 minutes（each）,for 15 days,taking a break every 7 *** Morris water maze test was conducted to assess the learning and *** expression levels of glycogen synthase kinase-3β（GSK-3β）,p Ser9-GSK-3β,p Tyr216-GSK-3β,amyloid precursor protein and Aβ_（1–40） in the hippocampus were determined by western blot *** demonstrated that electroacupuncture treatment at different frequencies markedly improved learning and memory ability,increased synaptic curvatures,decreased the width of synaptic clefts,thickened postsynaptic densities,and downregulated the expression of GSK-3β,amyloid precursor protein,and Aβ_（1–40）.pSer9-GSK-3βexpression markedly decreased,while p Tyr216-GSK-3βexpression ***-frequency（50 Hz）electroacupuncture was more effective than low（2 Hz）or medium-frequency（30 Hz）*** conclusion,electroacupuncture treatment exerts a protective effect against Aβ_（1–42）-induced learning and memory deficits and synapse-ultrastructure impairment via inhibition of GSK-3β***,high-frequency electroacupuncture was the most effective therapy.

关键词： nerve regeneration electroacupuncture different frequencies Alzheimer's disease cognitive impairment hippocampus glycogen synthase kinase-3β synaptic curvatures width of synaptic cleft postsynaptic density Baihui(GV20) Shenshu(BL23) neural regeneration

Dietary Supplementation of β-Carotene Reveals miRNAs Involved in the Regulation of Carotenoid Metabolism in Crassostrea gigas

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Journal of Ocean University of China 2024年第4期23卷 1045-1053页

作者： SUN Dongfang WAN Sai YU hong kong lingfeng li Qi Key Laboratory of Mariculture Ministry of EducationOcean University of ChinaQingdao 266003China Laboratory for Marine Fisheries Science and Food Production Processes Laoshan LaboratoryQingdao 266237China

Carotenoids play crucial physiological roles in animals.A comprehensive investigation into the mechanism of carotenoid metabolism in oysters will establish a theoretical foundation for further development of its carotenoid-rich ***,the information on the function of miRNA in β-carotene metabolism in oysters is *** elucidate the mechanisms underlying miRNA regulation of carotenoid metabolism in oysters,we compared the expressions of miRNA in digestive gland tissues of Pacific oyster(Crassostrea gigas)fed with aβ-carotene supplemented diet and a normal diet,respectively.A total of 690 candidate miRNAs in the Pacific oyster digestive gland tissues were identified,including 590 known miRNAs and 111 unknown *** differentially expressed miRNAs were obtained in the carotenoid-fed and normal groups,associated to 137 differentially expressed target ***,the GO enrichment analysis revealed that the differentially expressed target genes were mainly involved in transmembrane transport *** enrichment showed that the differentially expressed target genes were involved in ABC *** of the mRNA-miRNA network revealed that novel0025 played a central role in carotenoid metabolism,and it was negatively correlated with the expression of 46 *** addition,down-regulated expression of novel0025 upregulated the expression of the lipoprotein gene LOC105342186,suggesting a potential regulatory role in carotenoid *** results provide useful information for elucidating the miRNA regulation mechanism during carotenoids metabolism in the Pacific oyster.

关键词： Crassostrea gigas β-carotene microRNA carotenoid metabolism

Epigenetic Regulation of Amyloid-beta Metabolism in Alzheimer’s Disease

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Current Medical Science 2020年第6期40卷 1022-1030页

作者： Chuan HE Zhong-sheng HUANG Chao-chao YU Hai-hua WANG Hua ZhOU li-hong kong Hubei University of Chinese Medicine Wuhan 430060China Department of Tuina Shenzhen Traditional Chinese Medicine HospitalShenzhen 518000China The 4th Clinical College of Guangzhou University of Chinese Medicine Shenzhen 518000China Hospital of Traditional Chinese Medicine of Fengrun District Tangshan 064000China

Senile plaques(SPs)are one of the pathological features of Alzheimer’s disease(AD)and they are formed by the overproduction and aggregation of amyloid-beta(Aβ)peptides derived from the abnormal cleavage of amyloid precursor protein(APP).Thus,understanding the regulatory mechanisms during Aβ metabolism is of great importance to elucidate AD *** studies have shown that epigenetic modulation-including DNA methylation,non-coding RNA alterations,and histone modifications-is of great significance in regulating Aβ *** this article,we review the aberrant epigenetic regulation of Aβ metabolism.

关键词： Alzheimer’s disease amyloid-β epigenetics DNA methylation microRNAs histone modifications

Numerical manifold method for steady-state nonlinear heat conduction using Kirchhoff transformation

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Science China(Technological Sciences) 2024年第4期67卷 992-1006页

作者： ZHANG liMei kong Heng ZHENG hong Key Laboratory of Urban Security and Disaster Engineering Ministry of EducationBeijing University of TechnologyBeijing 100124China Beijing Municipal Construction Co. Ltd.Beijing 100048China

The numerical manifold method(NMM)introduces the mathematical and physical cover to solve both continuum and discontinuum problems in a unified *** this study,the NMM for solving steady-state nonlinear heat conduction problems is presented,and heat conduction problems consider both convection and radiation boundary ***,the nonlinear governing equation of thermal conductivity,which is dependent on temperature,is transformed into the Laplace equation by introducing the Kirchhoff *** transformation reserves linearity of both the Dirichlet and the Neumann boundary conditions,but the Robin and radiation boundary conditions remain ***,the NMM is employed to solve the Laplace equation using a simple iteration procedure because the nonlinearity focuses on parts of the problem domain ***,the temperature field is retrieved through the inverse Kirchhoff *** examples are analyzed,demonstrating the advantages of the Kirchhoff transformation over the direct solution of nonlinear equations using the NewtonRaphson *** study provides a new method for calculating nonlinear heat conduction.

关键词： numerical manifold method nonlinear heat conduction temperature-dependent thermal conductivity Kirchhoff transformation convection and radiation boundary conditions

Al_(1-x)Sc_(x)Sb_(y)N_(1-y):An opportunity for ferroelectric semiconductor field effect transistor

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Science China(Physics,Mechanics & Astronomy) 2024年第11期67卷 133-140页

作者： Shujin Guo Xianghua kong hong Guo College of Physics and Electronic Engineering Center for Computational SciencesSichuan Normal UniversityChengdu 610068China College of Physics and Optoelectronic Engineering Shenzhen UniversityShenzhen 518060China Department of Physics McGill UniversityMontreal H3A 2T8Canada

For the in-memory computation architecture,a ferroelectric semiconductor field-effect transistor(FeSFET)incorporates ferroelectric material into the FET channel to realize logic and memory in a single *** emerging groupⅢnitride material Al_(1-x)Sc_(x)N provides an excellent platform to explore FeSFET,as this material has significant electric polarization,ferroelectric switching,and high carrier ***,steps need to be taken to reduce the large band gap of~5 eV of Al_(1-x)Sc_(x)N to improve its transport property for in-memory logic *** state-of-the-art first principles analysis,here we predict that alloying a relatively small amount(less than~5%)of Sb impurities into Al_(1-x)Sc_(x)N very effectively reduces the band gap while maintaining excellent *** show that the co-doped Sb and Sc act cooperatively to give a significant band bowing leading to a small band gap of~1.76 eV and a large polarization parameter~0.87 C/m^(2),in the quaternary Al_(1-x)Sc_(x)Sb_(y)N_(1-y)*** Sb impurity states become more continuous as a result of interactions with Sc and can be used for impurity-mediated *** on the Landau-Khalatnikov model,the Landau parameters and the corresponding ferroelectric hysteresis loops are obtained for the quaternary *** findings indicate that Al_(1-x)Sc_(x)Sb_(y)N_(1-y)is an excellent candidate as the channel material of FeSFET.

关键词： ferroelectricity Al_(1-x)Sc_(x)N FeSFET

Preventive electroacupuncture reduces cognitive deficits in a rat model of D-galactose-induced aging

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Neural Regeneration Research 2021年第5期16卷 916-923页

作者： Chao-Chao Yu Chuan He Yan-Jun Du Shan Gao Yuan-Fang lin Shu-Qin Wang li Wang Jia Wang Xue-Song Wang Tao Jiang li-hong kong Department of Tuina Shenzhen Traditional Chinese Medicine HospitalShenzhenGuangdong ProvinceChina The 4th Clinical College Guangzhou University of Chinese MedicineShenzhenGuangdong ProvinceChina College of Acupuncture and Orthopedics Hubei University of Chinese MedicineWuhanHubei ProvinceChina Department of Acupuncture&Moxibustion Wuhan Hospital of Integrated Chinese&Western MedicineTongji Medical College of Huazhong University of Science&TechnologyWuhanHubei ProvinceChina

Acupuncture can reduce cognitive deficits in Alzheimer’s ***,whether electroacupuncture can prevent or alleviate the cognitive deficits in animal models of aging remains poorly *** have shown that disordered epigenetic modifications play a critical role in age-related cognitive ***,we hypothesized that preventive electroacupuncture might improve cognitive functions during aging by regulating epigenetic modifications.A rat model of aging was produced by intraperitoneal injection of 120 mg/kg D-galactose for 8 *** and Shenshu acupoints were stimulated by electroacupuncture for 8 weeks from the first day of D-galactose *** electroacupuncture alleviated memory impairment,decreased tau hyperphosphorylation,and reduced glycogen synthase kinase-3βprotein and mRNA expression levels in the brainstem dorsal raphe nucleus,where intracellular neurofibrillary tangle lesions first *** addition,the DNA methylation level in the promoter region of the glycogen synthase kinase-3βgene was *** effects of preventive electroacupuncture were stronger than those of preventive *** injection of 0.4 mg/kg 5-aza-2ʹ-deoxycytidine,an inhibitor of DNA methyltransferase that blocks epigenetic modifications,antagonized the effects of preventive *** results suggest that preventive electroacupuncture treatment alleviates cognitive impairment in aging rats probably by affecting the epigenetic modification of the glycogen synthase kinase-3βgene in the dorsal raphe *** study was approved by the Animal Ethics Committee of Hubei University of Chinese Medicine,China(approval ***201712001)on November 28,2017.

关键词： Alzheimer’s disease brain central nervous system cognitive impairment DNA methylation electroacupuncture neurological function pathways

Exploiting pattern and sustainable development of marginal lands in the Three Gorge Reservoir Areas

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Journal of Environmental Sciences 2001年第2期13卷 173-179页

作者： WU Gang, li Jing, kong hong mei, ZHAO Jing zhu, GAO lin (Department of Ecology System, Research Center for Eco Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China) Department of Ecology System Research Center for Eco-Environmental Sciences Chinese Academy of Sciences Beijing 100085 China.

This paper studied the distribution and utilization status, reasonable exploitation patterns and potential productivity, and sustainable development of marginal lands in the Three Gorge Reservoir Areas. It has been pointed that problems of the soil fertility degradation, poor productivity in crop lands, natural disasters and environmental deterioration existed in all the exploited marginal lands. In order to maintain the sustainability of the marginal lands, following measure have been proposed: prohibiting cultivation in the crop lands with a slope more than 25°, restoring vegetation in the slope more than 25°, strengthening the basic agricultural construction, establishing the production base of woody feeds and oils, establishing a multi dimensional management model with reasonable structure and optimum function for forestry, agriculture and fishing.

关键词： marginal lands utilization pattern sustainable development Three Gorge Reservoir Are4