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Inheritance and Molecular Marker of Resistance to Bot Canker in Malus domestica

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Agricultural Sciences in China 2011年第2期10卷 175-184页

作者： liU hai-tao li cai-li ZHANG Yu-jing li Chun-min ZHAO Yong-bo CHEN Dong-mei WANG Yi ZHANG Xin-zhong HAN Zhen-hai Institute for Horticultural Plants College of Agronomy and BiotechnologyChina Agricultural UniversityBeijing 100193P.R.China Changli Institute for Pomology Hebei Academy of Agriculture and Forestry SciencesChangli 066600P.R.China College of Horticulture Northeast Agricultural UniversityHarbin 150030P.R.China

Apple bot canker [Botryosphaeria dothidea（Moug.） *** de Not.] is distributed worldwide,resulting in a serious crop loss every year in apple（Malus domestica Borkh.） *** resistance of each seedling derived from a hybrid population（Jonathan×Golden Delicious） was evaluated by disease index either from natural infection in the field or from inoculation with five isolates of ***,Ls1,Lw023,Lw048,Mx1,and *** inheritance of the resistance to bot canker was analyzed via frequency distribution analysis,and microsatellite and AFLP markers linked to the resistance loci were *** the binary frequency distribution patterns,it was found that the segregation ratio of resistant/susceptible genotypes infected by pathogen isolates Lw023 and Ls1 was 1：15;and that by Zz26 and Mx1 was 15：*** variation of resistance was involved in the segregation of two to four alleles of major genes,the resistance was recessive when infected by Lw023 and Ls1,but was dominant when infected with Mx1 and Zz26.A microsatellite maker,CH02a04-450,and two AFLP markers,E-AG/M-GAC-280 and E-AGG/M-CTT-110,were identified,and their map distances to the resistance loci were 5.1,5.1 and 6.2 cM,*** three markers are located in different linkage groups,while CH02a04-450 is on linkage group 2 or 7.E-AG/M-GAC-280 was successfully converted into ***,CH02a04-450 and SCAR159 were re-examined in inoculated segregation population and presented a good reliability on predicting phenotypes of resistance.

关键词： Malus domestica Borkh. Botryosphaeria dothidea inheritance microsatellite AFLP

Removal of intraocular foreign body in anterior chamber angle with prism contact lens and 23-gauge foreign body forceps

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International Journal of Ophthalmology(English edition) 2017年第5期10卷 749-753页

作者： Yan-Ming Huang Hua Yan Jin-Hong cai hai-Bo li Department of Ophthalmology Tianjin Medical University General Hospital Tianjin Neurological Institue Key Laboratory of Post-neuroinjury Neuro-repair and Regeneration in Central Nervous System Ministry of Education and Tianjin City Department of Ophthalmology Xiamen Eye Center Affiliated to Xiamen University

AIM：To introduce a novel approach in removal of anterior chamber angle foreign body（ACFB） using a prism contact lens and 23-gauge foreign body ***：Data of 42 eyes of 42 patients who had undergone removal of ACFB using a prism contact lens and 23-gauge foreign body forceps from January 2008 to October 2013 were collected and analyzed. Twenty eyes in group A received the conventional approach by using toothed forceps through corneal limbus incision, and 22 eyes in group B underwent the novel method through the opposite corneal limbus incision. RESULTS：The success rate of ACFB once removal was 75%（15/20） in group A, and 100%（22/22） in group B. The average operation time of group A was significantly longer compared with group B（34.9±9.88 min vs 22.13±8.85min; P〈0.05）. The average size of corneal limbus incision in group A was significantly larger than that of group B（4.85±1.89 mm vs 3.95±1.17 mm; P〈0.05）. The corneal limbus incision suturing were conducted in all eyes in group A, and only 5 eyes in group B. CONCLUSION：Removal of ACFB using a prism contact lens and 23-gauge foreign body forceps is a safer, more effective, and convenient technique compared with the conventional approach.

关键词： prism contact lens intraocular foreign body anterior chamber angle 23-gauge foreign body forceps

Chinese Medicine Plaster as A New Treatment for Surgical Site Infection in Patients with Cesarean Delivery:A Randomized,Double-Blind,Controlled Trial

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Chinese Journal of Integrative Medicine 2023年第6期29卷 483-489页

作者： HAN Guan-ya WU Xiao-li li Dong-mei cai hai-rui ZHOU Jun-jun HE Xiao-bo Department of Obstetrics Ningbo Women and Children's HospitalNingboZhejiang Province(315012)China Department of Medical Intensive Care Unit Ningbo Women and Children's HospitalNingboZhejiang Province(315012)China

Objective To evaluate the efficacy of Chinese plaster containing rhubarb and mirabilite on surgical site infection(SSI)in patients with cesarean delivery(CD)by performing a randomized controlled *** This randomized controlled trial included 560 patients with CD due to fetal head descent enrolled at a tertiary teaching center between December 31,2018 and October 31,*** patients were randomly assigned to a Chinese medicine(CM)group(280 cases)or a placebo group(280 cases)by a random number table,and were treated with CM plaster(made by rhubarb and mirabilite)or a placebo plaster,*** courses of treatment lasted from the day 1 of CD,followed day 2 until *** primary outcome was the total number of patients with superficial,deep and organ/space *** secondary outcome was duration of postoperative hospital stay,antibiotic intake,and unplanned readmission or reoperation due to *** reported efficacy and safety outcomes were confirmed by a central adjudication committee that was unaware of the study-group *** During the recovery process after CD,the rates of localized swelling,redness and heat were significantly lower in the CM group than in the placebo group[7.55%(20/265)vs.17.21%(47/274),Plite had an effect on *** is safe for mothers and im

关键词： Chinese medicine plaster rhubarb and mirabilite surgical site infection cesarean delivery randomizedcontrolledtrial

Discovery of Natural Co-occurring Enantiomers of Monoterpenoid Indole Alkaloids

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Chinese Journal of Chemistry 2021年第4期39卷 866-872页

作者： Yang Yu Mei-Fen Bao Xiang-hai cai State Key Laboratory of Phytochemistry and Plant Resources in West China Kunming Institute of BotanyChinese Academy of SciencesKunmingYunnan650201 China

Main observation and conclusion Two new pairs of monoterpenoid indole alkaloids(MIAs)enantiomers as well as their new congeners were isolated from stems of Tabernaemontana bovina Lour.(Apocynaceae).Their structures were elucidated by spectroscopic analyses and confirmed by X-ray diffractions and computational *** A,tabovine B and tabovines C-D possessed unprecedented 6/5/5/6/6,6/5/5/6/5 and 6/5/6/6/6 ring systems,*** is the first time to report co-occurring enantiomers of ***,the explanation concerning biogenesis of MIAs was *** findings would open a new window where we could disclose more diverse MIAs.

关键词： Monoterpenoid indole alkaloid Enantiomers Tabovines A-D Structure elucidation Extraction and isolation

Electrocatalysis of Oxygen Evolution Reaction on Ti/SnO_2+RuO_2+MnO_2/MnO_2 Electrode in Sulfuric Acid Solution

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Chemical Research in Chinese Universities 2001年第3期17卷 287-292页

作者： liANG Zhen-hai FAN cai-mei SUN Yan-ping Laboratory of Electrochemical Engineering Research College of Chemical EngineeringTaiyuan University of TechnologyTaiyuan 030024P.R.China

The Ti-Supported MnO_2 electrode was modified by introducing SnO_2+RuO_2+MnO_2 as an intermediate layer into the Ti/MnO_2 interface. The anodic polarization curves were measured at various temperatures ranging from 30 to 80℃ and the activation energy for the oxygen evolution reaction was evaluated. The experimental activation energy increased linearly with increasing the overpotential. The activation energy at the equilibrium potential was linearly correlated with the difference between the crystal field stabilization energies of Mn^(4+) at initial state and Mn^(4+) at transition state. The electrocatalysis characteristics of the anode were discussed by means of the mechanism of the substitution reaction of the ligand(S_N1 and S_N2) and molecular orbital theory. The results show that the anode has better electrocatalystic characteristics.

关键词： Ti/SnO2+RuO2+MnO2/MnO2 anode Electroeatalysis Activation energy Crystal field theory Molecular Orbital theory

Diffusion of an Extra Ga Atom in GaAs（001）（2×4） Rich-As Surface

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Chinese Journal of Chemical Physics 2008年第1期21卷 69-75页

作者： Kun li Bi-cai Pan College of Physics and Engineering Qufu Normal University Qufu 273165 China Hefei National Laborator7 for Physical Sciences at Microscale Department of Physics University ofScience and Technology of China Hefei 230026 China

The potential energy surface for the migration of an extra Ga atom on the GaAs（001） β2（2×4） surfuce was mapped out by performing calculations at the level of analytical bond-order potential. Based on this calculations, we found some lower-energy sites for the adsorption of an extra Ga atom in the surface, which were in agreement with the experimental data. Moreover, many possible pathways for an extra Ga atom diffusing in this surface were revealed. According to the relative energies of the possible pathways, the individual Ga adatoms preferably keep their diffusion in two pathways parallel to the As dimers. This result can be understood using the strain caused by the diffusing Ga atom in the pathways. In addition, the simulated kinetic processes of the extra Ga atom diffusing in different pathways at finite temperatures support the prediction from our calculated potential energy surface.

关键词： Surface Adsorption Diffusion of an atom Molecular-dynamics simulation

Quality Changes and Safety Evaluation of Ready-to-Eat Roasted Antarctic Krill(Euphausia superba)During Storage at Room Temperature(25℃)

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Journal of Ocean University of China 2023年第1期22卷 235-241页

作者： YANG Xu SHI Yongfu cai Youqiong CHI hai East China Sea Fisheries Research Institute Chinese Academy of Fishery SciencesShanghai 200090China

The objective of this study was to determine the quality changes and shelf life of ready-to-eat roasted Antarctic krill,either untreated(CT)or treated with sodium diacetate(SD)or sodium diacetate and a deoxidizer(SDD),during storage at room temperature(25℃)by using sensory,physiochemical,and microbial ***,fluoride accumulation in this food product was used to evaluate its *** showed that the addition of SD and SDD resulted in better sensory scores compared of that of CT samples(Plife of ready-to-eat roasted Antarctic krill treated with SDD was extended by up to 52 ***,rats fed ready-to-eat roasted Antarctic krill showed accumulation of fluoride exclusively in the *** accumulation of fluoride residues in the thighbone showed *** concentrations of fluoride residues in rats were(1760.03±38.21),(2371.52±42.15)mgkg^(−1) and(3615.44±30.53)mgkg^(−1),which were less than sodium fluoride feeding group(4621.01±28.67)mgkg^(−1).The results suggested that the SD and SDD treatments led to better quality and shelf life extension

关键词： Antarctic krill ready-to-eat product antioxidant quality changes shelf life safety potential

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EEG-based Emotion Recognition Using Multiple Kernel Learning

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Machine Intelligence Research 2022年第5期19卷 472-484页

作者： Qian cai Guo-Chong Cui hai-Xian Wang School of Statistics and Data Science Nanjing Audit UniversityNanjing 211815China Key Laboratory of Child Development and Learning Science of Ministry of Education School of Biological Science&Medical EngineeringSoutheast UniversityNanjing 210096China Institute of Artificial Intelligence of Hefei Comprehensive National Science Center Hefei 230094China

Emotion recognition based on electroencephalography(EEG)has a wide range of applications and has great potential value,so it has received increasing attention from academia and industry in recent ***,multiple kernel learning(MKL)has also been favored by researchers for its data-driven convenience and high ***,there is little research on MKL in EEG-based emotion ***,this paper is dedicated to exploring the application of MKL methods in the field of EEG emotion recognition and promoting the application of MKL methods in EEG emotion ***,we proposed a support vector machine(SVM)classifier based on the MKL algorithm EasyMKL to investigate the feasibility of MKL algorithms in EEG-based emotion recognition *** designed two data partition methods,random division to verify the validity of the MKL method and sequential division to simulate practical ***,tri-categorization experiments were performed for neutral,negative and positive emotions based on a commonly used dataset,the Shanghai Jiao Tong University emotional EEG dataset(SEED).The average classification accuracies for random division and sequential division were 92.25%and 74.37%,respectively,which shows better classification performance than the traditional single kernel *** final results show that the MKL method is obviously effective,and the application of MKL in EEG emotion recognition is worthy of further *** the analysis of the experimental results,we discovered that the simple mathematical operations of the features on the symmetrical electrodes could not effectively integrate the spatial information of the EEG signals to obtain better *** is also confirmed that higher frequency band information is more correlated with emotional state and contributes more to emotion *** summary,this paper explores research on MKL methods in the field of EEG emotion recognition and provides a new way of thinking for EE

关键词： Emotion recognition electroencephalography(EEG) multiple kernel learning machine learning brain computer interface

Analysis of sensitivity to hydrate blockage risk in natural gas gathering pipeline

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Petroleum Science 2024年第4期21卷 2723-2733页

作者： Ao-Yang Zhang Meng cai Na Wei hai-Tao li Chao Zhang Jun Pei Xin-Wei Wang State Key Laboratory of Oil and Gas Reservoir Geology and Exploitation Southwest Petroleum UniversityChengdu610500SichuanChina Research Institute of Production Engineering Daqing Oilfield Co.Ltd.Daqing163453HeilongjiangChina

During the operational process of natural gas gathering and transmission pipelines,the formation of hydrates is highly probable,leading to uncontrolled movement and aggregation of *** continuous migration and accumulation of hydrates further contribute to the obstruction of natural gas pipelines,resulting in production reduction,shutdowns,and pressure ***,a cascade of risks is prone to *** address this issue,this study focuses on the operational process of natural gas gathering and transmission pipelines,where a comprehensive framework is *** framework includes theoretical models for pipeline temperature distribution,pipeline pressure distribution,multiphase flow within the pipeline,hydrate blockage,and numerical solution *** analyzing the influence of inlet temperature,inlet pressure,and terminal pressure on hydrate formation within the pipeline,the sensitivity patterns of hydrate blockage risks are *** research indicates that reducing inlet pressure and terminal pressure could lead to a decreased maximum hydrate formation rate,potentially mitigating pipeline blockage during natural gas ***,an increase in inlet temperature and terminal pressure,and a decrease in inlet pressure,results in a displacement of the most probable location for hydrate blockage towards the terminal ***,it is crucial to note that operating under low-pressure conditions significantly elevates energy consumption within the gathering system,contradicting the operational goal of energy efficiency and reduction of energy ***,for high-pressure gathering pipelines,measures such as raising the inlet temperature or employing inhibitors,electrical heat tracing,and thermal insulation should be adopted to prevent hydrate formation during natural gas ***,considering abnormal conditions such as gas well production and pipeline network shutdowns,which could potentially tr

关键词： Natural gas hydrates Gathering pipeline Temperature variation Hydrate formation rate Sensitivity analysis