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Identification of expressed genes in regenerating rat liver in 0-4-8-12 h short interval successive partial hepatectomy

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World Journal of Gastroenterology 2005年第15期11卷 2296-2305页

作者： Cun-ShuanXu Jin-YunYuan Wen-Qiangli Hong-PengHan Ke-JinYang Cui-FangChang li-fengzhao Yu-Changli Hui-YongZhang SalmanRahman Jing-BoZhang CollegeoflifeScience HenanNormalUniversityXinxiang453002HenanProvinceChina StateKeyLaboratoryofCellDifferentiationandRegulationofHenanProvinceandMinistry Xinxiang453002HenanProvinceChina HomophiliaResearchCenter LondonUniversityLondonSE17EHUK

AIM: To identify the genes differentially expressed in the regenerating rat liver of 0-4-8-12 h short interval successive partial hepatectomy (SISPH) and to analyze their expression ***: Five hundred and fifty-one elements screened from subtractive cDNA libraries were made into a cDNA microarray (cDNA chip). Extensive gene expression analysis following 0-4-8-12 h SISPH was conducted by ***: One hundred and eighty-three elements were selected, which were either up- or down-regulated more than 2-fold at one or more time points after SISPH. Cluster analysis and generalization analysis showed that there were five distinct temporal patterns of gene ***-six genes were unreported, associated with liver regeneration (LR).CONCLUSION: Microarray analysis shows that the down regulated genes are much more than the up-regulated ones in SISPH; the numbers of genes expressed consistently are fewer than that expressed immediately; the genes expressed in high abundance are much fewer than that increased 2-5-fold. The comparison of SISPH with partial hepatectomy (PH) shows that the expression trends of most genes in SISPH and in PH are similar,but the expression of 43 genes is specifically altered in SISPH.

关键词： SISPH liver regeneration

Expressed genes in regenerating rat liver after partial hepatectomy

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World Journal of Gastroenterology 2005年第19期11卷 2932-2940页

作者： Cun-ShuanXu Cui-FangChang Jin-YunYuan Wen-Qiangli Hong-PengHan Ke-JinYang li-fengzhao Yu-Changli Hui-YongZhang SalmanRahman Jing-BoZhang CollegeoflifeScience HenanNormalUniversityXinxiang453007HenanProvinceChina KeyLaboratoryforCellDifferentiationRegulation Xinxiang453007HenanProvinceChina HomophiliaResearchCenter LondonUniversityLondonSE17EHUK

AIM: To reveal the liver regeneration (LR) and its controlas well as the occurrence of liver disease and to study the gene expression profiles of 551 genes after partial hepatectomy (PH) in regenerating rat ***: Five hundred and fifty-one expressed sequence tags screened by suppression subtractive hybridization were made into an in-house cDNA microarray, and the expressive genes and their expressive profiles in regenerating rat livers were analyzed by microarray and bioinformatics. RESULTS: Three hundred of the analyzed 551 genes were up- or downregulated more than twofolds at one or more time points during LR. Most of the genes were up- or downregulated 2-5 folds, but the highest reached 90 folds of the control. One hundred and thirty-nine of themshowed upregulation, 135 displayed downregulation, and up or down expression of 26 genes revealed a dependence on regenerating livers. The genes expressedin 24-h regenerating livers were much more than those in the others. Cluster analysis and generalization analysis showed that there were at least six distinct temporal patterns of gene expression in the regenerating livers, that is, genes were expressed in the immediate early phase, early phase, intermediate phase, early-late phase, late phase, terminal phase. CONCLUSION: In LR, the number of down-regulated genes was almost similar to that of the upregulated genes; the successively altered genes were more than the rapidly transient genes. The temporal patterns of gene expression were similar 2 and 4 h, 12 and 16 h, 48 and 96 h, 72 and 144 h after PH. Microarray combined with suppressive subtractive hybridization can effectively identify the genes related to LR.

关键词： Subtracted cDNA libraries Complementary DNA microarray liver regeneration Partial hepatectomy Cluster analysis

CRYSTAL DEFECTS AND GRAIN INTERFACE STRUCTURE OF ION-NITRIDED LAYERS

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Acta Metallurgica Sinica(English Letters) 1993年第6期6卷 358-362页

作者： SUN Dongsheng li fengzhao Shandong Polytechnic University,Jinan Laboratory of Atomic Imaging of Solids,Institute of Metal Research,Academia Sinica,Shenyang,ChinaDAI Jiyan li Douxing Laboratory of Atomic Imaging of Solids,Institute of Metal Research,Academia Sinica,Shenyang,China associate professor,Institute of Materials Engineering,Shandong Polytechnic University,Jinan 250014,China

Observation under high resolution electron microscope shows that the continuous bombing of high speed ions produces a great amount of vacant site *** assembly of vacancies forms vacant dish,and the collapase of vacant dish forms stacking fault tetrahedrons and oth- er crystal *** interfaces between phase ε(Fe_(2-3)N)and phase γ'(Fe_4N)are smooth, straight and coherent,and they have the orientation relationships of(11)//(0001)and [110]/[110] .

关键词： crystal defect interface structure ion-nitriding steel 35CrMo

Gene expression differences of regenerating rat liver in a short interval successive partial hepatectomy

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World Journal of Gastroenterology 2004年第18期10卷 2680-2689页

作者： Cun-ShuanXu An-ShiZhang Hong-PengHan Jin-YunYuan Cui-FangChang wen-Qiangli Ke-JinYang li-fengzhao Yu-Changli Hui-YongZhang SalmanRahman Jing-BoZhang CollegeoflifeSciences HenanNormalUniversityXinxiang453007HenanProvinceChina StateKeyLaboratoryofCellDifferentiationandRegulationofProvinceandMinistry Xinxiang453007HenanProvinceChina HomophiliaResearchCenter LondonUniversityLondonSE17EHUnitedKingdom

AIM: To identify the genes expressed differentially in the regenerating rat liver in a short interval successive partial hepatectomy (SISPH), and to analyze their expression ***: Five hundred and fifty-one elements selected from subtractive cDNA libraries were conformed to a cDNA microarray (cDNA chip). An extensive gene expression analysis following 0-36-72-96-144 h SISPH was performed by ***: Two hundred and sixteen elements were identified either up- or down-regulated more than 2-fold at one or more time points of SISPH. By cluster analysis and generalization analysis, 8 kinds of ramose gene expression clusters were generated in the SISPH. Of the 216 elements,111 were up-regulated and 105 down-regulated. Except 99 unreported genes, 117 reported genes were categorized into 22 groups based on bheir biological functions. Comparison of the gene expression in SISPH with that after partial hepatectomy (PH) disclosed that 56 genes were specially altered in SISPH, and 160 genes were simultaneously upregulated or down-regulated in SISPH and after PH, but in various amount and at different time ***: Genes expressed consistently are far less than that intermittently, the genes strikingly increased are much less than that increased only 2-5 fold; the expression trends of most genes in SISPH and in PH are similar, but the expression of 56 genes is specifically altered in *** combined with suppressive subtractive hybridization can in a large scale effectively identify the genes related to liver regeneration.

关键词：基因表达表达差异新生老鼠肝脏局部肝切除术手术治疗

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城市轨道交通系统列车负荷分布模型

天津理工学院学报 1994年第4期10卷 11-16页

作者：李凤沼天津理工学院一分院

本文介绍地铁／轻轨负荷过程仿真软件中使用的列车负荷分布数学模型．给出了瞬时负荷图序列的数据结构及单元生成的伪代码．模型为在城市轨道运输供电系统分析创造了条件．

关键词：地下铁路轻轨电气化铁供电系统列车负荷

高纯度Fe－C－Mo合金贝氏体相变和形貌特征

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金属热处理学报 1995年第1期16卷 6-12页

作者：李凤照敖青山东工业大学

研究了高纯度Ｆｅ－０．０６％Ｃ－１．８％Ｍｏ和Ｆｅ－０．１９％Ｃ－１．８１％Ｍｏ合金恒温贝氏体转变。结果表明，５５０～５８０℃转变是完全的。两种合金分别在６００℃和５８５℃左右保温时，转变中途会出现暂时停滞现象，而后... 详细信息

研究了高纯度Ｆｅ－０．０６％Ｃ－１．８％Ｍｏ和Ｆｅ－０．１９％Ｃ－１．８１％Ｍｏ合金恒温贝氏体转变。结果表明，５５０～５８０℃转变是完全的。两种合金分别在６００℃和５８５℃左右保温时，转变中途会出现暂时停滞现象，而后恢复并加快转变。两合金ＴＴＴ图均有一明显稳定区（称为河湾或Ｔ_ｂ）。７５０℃保温形成铁素体和平行于奥氏体晶界的片状或杆状碳化物沉淀，７００～６００℃保温在较高碳量和用量形成少量节瘤状贝氏体，７００℃保温转变后可观察到珠光体，Ｔ_ｂ温度上下铁素体形貌明显不同，ｔ_ｂ以下形成的贝氏体铁素体为变异型，在晶内生成。

关键词：贝氏体碳化物相变黑色金属合金形貌

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硅酸钠—钾—铝三元复合水玻璃砂的试验研究

热加工工艺 1989年第5期18卷 30-33页

作者：李风藻王伯印邢铁海太原工业大学太原机械学院

在传统的CO_2法硅酸钠水玻璃型砂基础上引入硅酸钾水玻璃及铝酸钠溶液,配制成硅酸钠—钾—铝三元复合水玻璃粘结剂,试验表明,其湿压强度要比钠水玻璃高50～80%,干强度介于钠、钾水玻璃之间,表面强度高,溃散性良好。

关键词：水玻璃砂硅酸钠硅酸钾铝酸钠

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奥氏体钢排气阀离子软氮化的研究

金属热处理 1986年第6期 30-35页

作者：李凤照乔毅南敖青李合兴刘国兴山东工学院济南柴油机厂

4Cr14Ni14W2Mo奥氏体耐热钢在进行离子软氮化时,其保温时间只需离子氮化的一半,试样的耐磨性可提高两倍,耐蚀性可提高一倍,其表层组织主要是Fe2N、Fe3N和Cr2（CN）化合物。离子软氮化的渗层生长速度呈抛物线规律。

关键词：离子软氮化渗层扩散层奥氏体耐热钢表面层奥氏体钢离子氮化渗氮化合物层排气阀

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反应挤出在尼龙聚合与改性中的应用进展

工程塑料应用 2023年第6期51卷 146-150页

作者：王学军李锋招杨峰中国平煤神马集团蓝天化工股份有限公司河南驻马店463100 炼焦煤资源绿色开发全国重点实验室河南平顶山467000

介绍了反应挤出技术在工业路线设计和开发中的重要性,总结了反应挤出的技术进展及其在材料加工及功能化方面的优势,包括不使用溶剂、反应加工同步、连续生产、调控灵活、适合高黏体系等。详细回顾了反应挤出在尼龙挤出聚合、扩链支化、... 详细信息

介绍了反应挤出技术在工业路线设计和开发中的重要性,总结了反应挤出的技术进展及其在材料加工及功能化方面的优势,包括不使用溶剂、反应加工同步、连续生产、调控灵活、适合高黏体系等。详细回顾了反应挤出在尼龙挤出聚合、扩链支化、增容共混、微结构调控、纳米共混、材料增韧、阻燃改性、膜材料功能化和塑料回收等方面的应用研究进展。讨论了反应挤出存在的问题及面临的挑战,指出反应挤出未来的研究方向是多尺度模型模拟与实验验证、快速反应与传热限制、特异性与通用性、反应体系与工艺参数的定性甚至定量关系等。

关键词：尼龙反应挤出聚合改性升级循环