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Acta Mechanica Sinica 2011年第6期27卷 1081-1090页

作者： li Wang Mei-Wen An Xiao-Na li fang Yang Yang liu Institute of Applied Mechanics and Biomedical Engineering Taiyuan University of Technology 030024 Taiyuan China

To probe the contributions of polar cortical cytoskeleton and the surface tension of daughter cells to intercellular bridge thinning dynamics during cytokinesis,we applied cytochalasin D（CD） or colchicine（COLC） in a highly localized manner to polar regions of dividing normal rat kidney（NRK） *** observed cellular morphological changes and analyzed the intercellular bridge thinning trajectories of dividing cells with different polar cortical *** blebbistatin（BS） application was used to obtain cells losing active contractile force *** results show that locally released CD or colchicine at the polar region caused inhibition of cytokinesis before *** treatment at phases after ingression allowed completion of cytokinesis but dramatically influenced the trajectories of intercellular bridge *** single polar cortical actin induced transformation of the intercellular bridge thinning process,and polar cortical tension controlled deformation time of intercellular *** study provides a feasible framework to induce and analyze the effects of local changes in mechanical properties of cellular components on single cellular cytokinesis.

关键词： Cytokinesis Normal rat kidney epithelial cells Intercellular bridge Polar cortical cytoskeleton - Cortical surface tension

Hardy-Orlicz Spaces and Their Multiplication Operators

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Acta Mathematica Sinica,English Series 2005年第3期21卷 593-598页

作者： Qun LU Guang Fu CAO li fang liu Department of Mathematics Sichuan University Chengdu 610064 P. R. China Department of Mathematics Xiamen University Xiamen 361005 P. R. China

In this paper some formulae on the relationship between Hardy and Hardy Orlicz spaces are presented, and multiplication operators on Hardy-Orlicz spaces are discussed.

关键词： φ-function Nevalinna class and class N' Hardy-Orlicz space Condition △2 Multiplication operator

Reliability of Tonolab measurements in rats

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International Journal of Ophthalmology(English edition) 2014年第6期7卷 930-934页

作者： li-fang liu Chu-Kai Huang Ming-Zhi Zhang Joint Shantou International Eye Center of Shantou University and the Chinese University of Hong Kong

AIM: To assess the repeatability and reproducibility of Tonolab tonometer in rats with high intraocular pressure(IOP) and evaluate its ability to detect IOP changes in rats with general ***: Left eyes of adult Fischer rats(F344) were photocoagulated by 532 nm diode laser to induce high IOP. Hypertensive eyes of 30 conscious rats were randomly chosen to measure IOP on a single *** observers independently and alternately undertook IOP measurements consecutively for three times using the same Tonolab tonometer blind to the other observer’s IOP measurements. The within subject standard deviation(Sw), coefficient of variation(CVw)(100 ×Sw/overall mean), and intraclass correlation coefficient(ICC) were calculated to evaluate intra-observer repeatability. Inter-observer difference was analysed by using 95% limits of agreement described by Bland-Altman and paired sample t- test. Also, another13 normal F344 rats were intraperitoneally administrated with ketamine/xylazine or chloral hydrate, and IOPs of both eyes were measured by a single operator once every 5min until animals came to conscious. IOPs at various time points were compared by using one-way ***: Mean IOP was 35.58 mm Hg(range 17.33 to65.33 mm Hg). For intraobserver repeatability, the Sw,CVw and ICC of high IOP for two observers were 5.20 mm Hg/3.41 mm Hg, 9.98% /8.08% and 0.820/0.928 *** inter-observer difference was 14.76% ±19.76% of the mean IOP of two observers, with a 95% limits of agreement-23.97% to 53.50%, and the difference between mean IOP of these two observers was statistically significant(P =0.001). IOPs dropped slightly during the first 15 min post-aneathesia, with a IOP change between0.17 and 1.17 mm Hg. IOPs changed from basline of11.75 ±2.05 mm Hg(n =12) to 8.75 ±1.06 mm Hg 20 min post-anesthesia(P =0.001), and this hypotensivecondition persisted until 80 min post-anesthesia. CONCLUSION: In this sample of hypertensive rats,Tonolab measurements demon

关键词： Tonolab repeatability reproducibility rats

Clinical and pathological portraits of axillary presentation breast cancer and effects of preoperative systemic therapy

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Chinese Journal of Cancer Research 2017年第4期29卷 369-373页

作者： ling Xu fang li Yinhua liu Xuening Duan Jingming Ye Yuanjia Cheng ling Xin Breast Disease Center of Peking University First Hospital Beijing 100034China Department of General Surgery Beijing Aerospace General HospitalBeijing 100076China

There is a lack of investigation into the biological characteristics and preoperative systemic therapy （PST） for occult breast cancer （OBC）. For this study, departmental records in Breast Disease Center of Peking University First Hospital from January 2008 to December 2015 were retrospectively reviewed to identify cases of OBC. Eleven cases were included, and all patients were female, with a median age of 56 （range： 29-75） years. The sensitivity of magnetic resonance imaging （MRD was I00%, and the false positive rate was 33.3%. Based on histologic analysis of the axillary node, 9 （81.8%） cases were grade 3, and 2 （18.2%） cases were grade 2; 4 （36.4%） cases were 〉10% estrogen receptor （ER） positive and 6 （54.5%） human epidermal growth receptor 2 （HER2） positive. Nine cases （81.8%） exhibited over 30% Ki67 expression. PST was performed in 5 of the 11 cases. The lymph node response rate was 100% （5/5）, but no complete remission was achieved. In conclusion, aggressive subtypes were predominant among the included cases, and PST should be considered for OBC treatment options.

关键词： AxiUary presentation breast cancer occult breast cancer (OBC) magnetic resonance imaging (MRI) preoperative systemic therapy (PST)

Comparison of OA-2000 and IOL Master 500 using in cataract patients with high myopia

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International Journal of Ophthalmology(English edition) 2019年第5期12卷 844-847页

作者： Ya-li Du Geng Wang Hui-Chun Huang li-Yu lin Chuang Jin li-fang liu Xiu-Ru liu Ming-Zhi Zhang The Joint Shantou International Eye Center of Shantou University and the Chinese University of Hong Kong

This study was designed to compare optical biometry measurements and predicted refraction in cataract patients with high myopia [axial length(AL) ≥26 mm] using OA-2000 and IOL Master 500. Ocular biometry measurements were performed using both biometers before surgery. Uneventful cataract surgery was performed in all patients. Postoperative manifest refraction was obtained 3 wk after surgery or later. A total of 67 eyes were examined. The AL, keratometry(K), and anterior chamber depth(ACD) of the two biometers showed excellent agreement. Predicted errors were similar and a strong positive correlation was observed(r=0.909). Out of 21 eyes(31.34%) with unsuccessful AL readings using the IOL Master 500, 20 eyes of them could be measured using OA-2000. Therefore, the biometric parameters measured by the two biometers showed good agreement. However, OA-2000 had a lower AL measurement failure rate.

关键词： OA-2000 IOL Master 500 biometry measurement IOL power calculation high myopia cataract

Novel insights into stress-induced susceptibility to influenza:corticosterone impacts interferon-βresponses by Mfn2-mediated ubiquitin degradation of MAVS

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Signal Transduction and Targeted Therapy 2020年第1期5卷 782-793页

作者： Zhuo Luo li-fang liu Ying-Nan Jiang Lu-Ping Tang Wen li Shu-Hua Ouyang Long-fang Tu Yan-Ping Wu Hai-Biao Gong Chang-Yu Yan Shan Jiang Yu-Hui Lu Tongzheng liu Zhenyou Jiang Hiroshi Kurihara Yang Yu Xin-Sheng Yao Yi-fang li Rong-Rong He Guangdong Engineering Research Center of Chinese Medicine&Disease Susceptibility Jinan UniversityGuangzhou 510632China International Cooperative Laboratory of Traditional Chinese Medicine Modernization and Innovative Drug Development of Chinese Ministry of Education(MOE) College of PharmacyJinan UniversityGuangzhou 510632China Guangdong Province Key Laboratory of Pharmacodynamic Constituents of TCM and New Drugs Research College of PharmacyJinan UniversityGuangzhou 510632China School of Traditional Chinese Materia Medica Shenyang Pharmaceutical UniversityShenyang 110016China Institute of Tumor Pharmacology College of PharmacyJinan UniversityGuangzhou 510632China Department of Microbiology and Immunology Basic Medicine CollegeJinan UniversityGuangZhou 510632China

Although stress has been known to increase the susceptibility of pathogen infection,the underlying mechanism remains *** this study,we reported that restraint stress dramatically enhanced the morbidity and mortality of mice infected with the influenza virus(H1N1)and obviously aggravated lung ***(CORT),a main type of glucocorticoids in rodents,was secreted in the plasma of stressed *** further found that this stress hormone significantly boosted virus replication by restricting mitochondrial antiviral signaling(MAVS)protein-transduced IFN-βproduction without affecting its mRNA level,while the deficiency of MAVS abrogated stress/CORT-induced viral susceptibility in ***,the effect of CORT was mediated by proteasome-dependent degradation of MAVS,thereby resulting in the impediment of MAVS-transduced IFN-βgeneration in vivo and in ***,RNA-seq assay results indicated the involvement of Mitofusin 2(Mfn2)in this ***-and loss-offunction experiments indicated that Mfn2 interacted with MAVS and recruited E3 ligase SYVN1 to promote the polyubiquitination of ***-immunoprecipitation experiments clarified an interaction between any two regions of Mfn2(HR1),MAVS(C-terminal/TM)and SYVN1(TM).Collectively,our findings define the Mfn2-SYVN1 axis as a new signaling cascade for proteasome-dependent degradation of MAVS and a‘fine tuning’of antiviral innate immunity in response to influenza infection under stress.

关键词： influenza ubiquitin MAVS

Behavioral responses for evaluating the attractiveness of specific tea shoot volatiles to the tea green leafhopper, Empoaca vitis

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Insect Science 2012年第2期19卷 229-238页

作者： Dan Mu lin Cui Jian Ge Meng-Xin Wang li-fang liu Xiao-Ping Yu Qing-He Zhang Bao-Yu Han Tea Research Institute of Chinese Academy of Agricuitural Sciences Zhejiang Provincial Key Laboratory of Biometrology and Inspection&Quarantine College of Life Sciences of China Jiliang UniversityHangzhouChina Sterling International Inc.SpokaneWAUSA

The tea green leafhopper, Empoasca vitis Gothe, is one of the most serious insect pests of tea plantations in China's Mainland. Over the past decades, this pest has been controlled mainly by spraying pesticides. Insecticide applications not only have become less effective in controlling damage, but even more seriously, have caused high levels of toxic residues in teas, which ultimately threatens human health. Therefore, we should seek a safer biological control approach. In the present study, key components of tea shoot volatiles were identified and behaviorally tested as potential leafhopper attractants. The following 13 volatile compounds were identified from aeration samples of tea shoots using gas chromatography-mass spectrometry （GC-MS）：（E）-2-hexenal, （Z）-3-hexen-1- ol, （Z）-3-hexenyl acetate, 2-ethyl-1-hexanol, （E）-ocimene, linalool, nonanol, （Z）-butanoic acid, 3-hexenyl ester, decanal, tetradecane, β-caryophyllene, geraniol and hexadecane. In Y-tube olfactometer tests, the following individual compounds were identified：（E）-2- hexenal, （E）-ocimene, （Z）-3-hexenyl acetate and linalool, as well as two synthetic mixtures （called blend 1 and blend 2） elicited significant taxis, with blend 2 being the most attractive. Blend 1 included linalool, （Z）-3-hexen-l-ol and （E）-2-hexenal at a 1： 1：1 ratio, whereas blend 2 was a mixture of eight compounds at the same loading ratio：（E）-2-hexenal, （Z）- 3-hexen-l-ol, （Z）-3-hexenyl acetate, 2-penten-l-ol, （E）-2-pentenal, pentanol, hexanol and 1-penten-3-ol. In tea fields, the bud-green sticky board traps baited with blend 2, （E）-2- hexenal or hexane captured adults and nymphs of the leafhoppers, with blend 2 being the most attractive, foUowed by （E）-2-hexenal and hexane. Placing sticky traps baited with blend 2 or （E）-2-hexenal in the tea fields significantly reduced leathopper populations. Our results indicate that the bud-green sticky traps baited with tea shoot volatiles can provide a new tool for monitoring and managing the

关键词： attractant behavior green leaf volatiles tea green leafhopper tea shoot volatiles

Phosphorus Fractions in Water and Sediment and Their Correlations in Hengshui Lake, China

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Journal of Environmental Science and Engineering(A) 2016年第7期5卷 356-361页

作者： Zhanchen li fang liu Zhe Qin Shuxuan liang College of Chemistry and Environmental Science Hebei University Baoding 071002 China

Sediment is a principal reservoir which accumulates and releases the pollutants. It is an important way of nutrients releasing from pore water of sediments to overlying water across the sediment-water interface. Five sampling sites were selected in the Hengshui Lake. Overlying water and sediments were collected in each of them. The phosphorus fractions and their relationships were analyzed. The results showed that the change of the total phosphorus （TP） in sediment was similar to that of the total dissolved phosphorus （TDP） and soluble orthophosphate （PO4^3-） in pore water. The concentrations of PO4^3- in pore water were higher than those in overlying water, reflecting a possible diffuse trend for PO4^3-P in the five zones of Hengshui Lake. It is indicated that phosphorus and other nutrients will be transferred from sediment to pore water and then to overlying water, which can lead to lake eutrophication.

关键词： Sediment pore water overlying water phosphorus fractions Hengshui Lake.

Performance of brain MRI of a two generation father-and-son family with long-term smoking caffeinum natrio benzoicum

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Chinese Medical Journal 2010年第24期123卷 3714-3716页

作者： SUN Wan-li ZHANG li-fang liu Hong ZHAO li-yun XING Yu-ping YUAN Ning Department of Radiology Heping Hospital Affiliated to Changzhi Medical College Changzhi Shanxi 046000 China

Since the 1970s, some people in north, northeast,northwest areas began to smoke caffeinum natrio benzoicum （CNB） and started to abuse CNB. As a common ingredient that can be found in drugs or drinks,many studies on the safety of CNB have been investigated, but no one has shown significant abnormality in the brain of a CNB abuser by using neuroimaging. In our study, three subjects from of a two generation father-and-son family with long-term smoking CNB were enrolled. Combining with the clinical data and taking the cocaine animal trial model and toxicology as the reference, we wanted to observe the performance of brain MRI and MRS of patients with long-term smoking CNB.

关键词： long-term smoking caffeinum natrio benzoicum magnetic resonance imaging proton magnetic resonance spectroscopy

Effect of Arctium lappa *** the dextran sulfate sodium colitis mouse model

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World Journal of Gastroenterology 2010年第33期16卷 4193-4199页

作者： Tzou-Chi Huang Shinn-Shyong Tsai li-fang liu Yu lin liu Hung-Jen liu Kuo Pin Chuang Department of Food Science National Pingtung University of Science and Technology Department of Veterinary Medicine Department of life Science National Pingtung University of Science and Technology912PingtungTaiwanChina Graduate Institute of Animal Vaccine Technology National Pingtung University of Science and Technology912PingtungTaiwanChina Institute of Molecular Biology National Chung HsingUniversity250Kuo Kung Rd.TaichungTaiwanChina

AIM:To analyze the possible protective role of Arctium lappa L.(AL)in a murine model of ulcerative colitis(UC).METHODS:BALB/c mice were administered 100 mg/kg AL powder orally each *** 7 d,colitis was induced by administration of dextran sulfate sodium(DSS)(5% W/V)in drinking water for a further 8 consecutive *** and bloody stools as well as colonic histology were *** level of interleukin-6(IL-6)and tu-mor necrosis factor-α(TNF-α)in colonic sections were detected by ***:There were significant differences in mean body weight values and disease activity indices between controls and AL-treated ***,the histological findings showed that AL treatment can prevent mucosal edema,submucosal erosions,ulceration,inflammatory cell infiltration and colon *** addition,immunohistochemistry analysis showed that the levels of the inflammatory cytokines,IL-6 and TNF-α were also decreased in AL-treated ***:We suggest that AL can prevent intestinal damage and decrease inflammatory cytokines in mice with DSS-induced ***,AL could prove to be a useful food for UC.

关键词： Arctium lappa L. Colitis Cytokine Inflam- matory bowel disease Ulcerative colitis