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Cloning, expression and chitin-binding activity of two peritrophin-like protein genes in the common cutworm, Spodoptera fitura

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Insect Science 2014年第4期21卷 449-458页

作者： Wei-Jun Chen li-Xia huang dan hu li-Yan liu Jun Gu li-hua huang Qi-li Feng Guangdong Provincial Key Laboratory of Biotechnology for Plant Development School of Life Sciences South China Normal UniversityGuangzhou China

Insect midgut secretes a semi-permeable peritrophic membrane （PM）, which plays important roles in protecting the midgut and helping with food digestion. The lep- idopteran larvae produce type 1 PM, which is degraded when insects develop into the metamorphic stages. However, the underlying mechanism is unclear. In the present study, two peritrophin-like proteins （peritrophin-57 and 37） were identified from the midgut expression sequence tag library and transcriptome of the common cutworm, Spodoptera litura. The temporal and spatial expression patterns and responses to the induction of 20- hydroxyecdysone （20E） and starvation were examined by real-time quantitative polymerase chain reaction according to their common sequence region. The chitin-binding activity was also studied using a competitor, calcofluor. The open reading frames are 1 554 and 1 020 bp, respectively. They shared four highly conserved peritrophin-A domains and were ex- pressed only in the midgut rather than in the other tissues, including fat body, epidermis, Malpighian tube and hemolymph. Their transcriptional expression could only be detected at the larval stages rather than in eggs, prepupae, pupae and adults. The purified protein of peritrophin-37 bound to chitin in a dose-dependent manner. These results indicate that the two proteins are peritrophins, the structural components of PM. In addition, the messenger RNA levels of the two peritrophins were significantly down-regulated by 20E injection, whereas feeding/starvation had no effect on the expression. These findings suggest that the increase of20E titer may be an important factor which controls the degradation of PM during metamorphosis.

关键词： metamorphosis peritrophic membrane peritrophin starvation 20-hydroxyecdysone

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Beta-blockers and 1-year clinical outcomes in hospitalized heart failure patients with atrial fibrillation

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Journal of Geriatric Cardiology 2021年第9期18卷 728-738页

作者： Fu-Wei XING li-hua ZHANG Hai-Bo ZHANG Xue-Ke BAI dan-li hu Xin ZHENG Jing li National Clinical Research Center for Cardiovascular Diseases NHC Key Laboratory of Clinical Research for Cardiovascular MedicationsState Key Laboratory of Cardiovascular DiseaseFuwai HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeNational Center for Cardiovascular DiseasesBeijingChina

OBJECTIVE To assess the association between beta-blockers and 1-year clinical outcomes in heart failure(HF)patients with atrial fibrillation(AF),and further explore this association that differs by left ventricular ejection fraction(LVEF)*** We enrolled hospitalized HF patients with AF from China Patient-centered Evaluative Assessment of Cardiac Events Prospective Heart Failure *** proportional hazard regression models were employed to calculate hazard ratio of *** primary outcome was all-cause *** Among 1762 HF patients with AF(756 women[41.4%]),1041(56%)received beta-blockers at discharge and 1272(72.2%)had an LVEF>40%.During one year follow up,all-cause death occurred in 305(17.3%),cardiovascular death occurred in203 patients(11.5%),and rehospitalizations for HF occurred in 622 patients(35.2%).After adjusting for demographic characteristics,social economic status,smoking status,medical history,anthropometric characteristics,and medications used at discharge,the use of beta-blockers at discharge was not associated with all-cause death[hazard ratio(HR):0.86;95%Confidence Interval(CI):0.65-1.12;P=0.256],cardiovascular death(HR:0.76,95%CI:0.52-1.11;P=0.160),or the composite outcome of all-cause death and HF rehospitalization(HR:0.97,95%CI:0.82-1.14;P=0.687)in the entire *** were no significant interactions between use of beta-blockers at discharge and LVEF with respect to all-cause death,cardiovascular death,or composite *** the adjusted models,the use of beta-blockers at discharge was not associated with all-cause death,cardiovascular death,or composite outcome across the different levels of LVEF:reduced(linical outcomes,regardless of LVEF.

关键词： Beta-blockers and 1-year clinical outcomes in hospitalized heart failure patients with atrial fibrillation

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Cataract-causing mutation S228P promotes βB1-crystallin aggregation and degradation by separating two interacting loops in C-terminal domain

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Protein & Cell 2016年第7期7卷 501-515页

作者： liang-Bo Qi li-dan hu huihui liu Hai-Yun li Xiao-Yao Leng Yong-Bin Yan State Key Laboratory of Membrane Biology School of Life Sciences Tsinghua University Beijing 100084 China

β/γ-Crystallins are predominant structural proteins in the cytoplasm of lens fber cells and share a similar fold composing of four Greek-key motifs divided into two domains. Numerous cataract-causing mutations have been identified in various β/γ-crystallins, but the mech- anisms underlying cataract caused by most mutations remains uncharacterized. The S228P mutation in βB1- crystallin has been linked to autosomal dominant con- genital nuclear cataract. Here we found that the S228P mutant was prone to aggregate and degrade in both of the human and E. coli cells. The intracellular S228P aggregates could be redissolved by lanosterol. The S228P mutation modified the refolding pathway of βB1- crystallin by affecting the formation of the dimeric intermediate but not the monomeric intermediate. Compared with native βBl-crystallin, the refolded S228P protein had less packed structures, unquenched Trp fluorophores and increased hydrophobic exposure. The refolded S228P protein was prone to aggregate at the physiological temperature and decreased the protective effect of βB1-crystallin on βA3-crystallin. Molecular dynamic simulation studies indicated that the mutation decreased the subunit binding energy and modified the distribution of surface electrostatic potentials. More importantly, the mutation separated two interacting loops in the C-terminal domain, which shielded the hydrophobic core from solvent in native βB1-crystallin. These two interacting loops are highly conserved in both of the N- and C-terminal domains of all β/γ-crys- tallins. We propose that these two interacting loops play an important role in the folding and structural stability of β/γ-crystallin domains by protecting the hydrophobic core from solvent access.

关键词： β/γ-crystallin cataract-causing mutation,hydrophobic core protein aggregation protein folding

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Highly efficient detection of insulinotropic action of glucagon via GLP-1 receptor in mice pancreatic beta-cell with a novel perfusion microchip

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Chinese Chemical Letters 2016年第7期27卷 1027-1031页

作者： li-dan hu Yu-lin Zhang Hong Wang Xing-Yue Peng Yi Wang School of life Sciences. Xiamen University. Xiamen 361005. China Laboratory of Cardiometabolic Molecular Medicine Institute of Molecular Medicine Peking University Beijing 100871 China College of life Sciences and Technology Huazhong University of Science and Technology Wuhan 430074 China

Glucagon exhibits insulinotropic ability by activating cAMP through glucagon or glucagon-like peptide-1（GLP-1） *** investigate the mechanism of endogenous and exogenous glucagon on insulin release,we studied the receptor selectivity on pancreatic islet beta-cells by switching the glucose concentration from 20 mmol/L to 0 mmol/L To measure the exact temporal relationship between glucagon and insulin release,we developed a quick,small volume,multi-channel polydimethylsiloxane（PDMS） *** 0 mmol/L glucose,we observed an insulinotropic effect in both INS-1 cells and ***,we observed a 63 ± 6.27 s delay of endogenous glucagon-induced insulin *** treatment with glucagon and GLP-1 receptor antagonists,we found that endogenous glucagon utilized the glucagon receptor,whereas exogenous glucagon primarily utilized the GLP-1 receptor to promote insulin *** microchip can also be used to describe the ＂glucagonocentric＂ vision of diabetes *** together,the insulinotropic mechanism of different receptors should be taken into account in clinical treatments.

关键词： Glucagon Insulin Glucagon receptor GLP-1 receptor Microchip

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LM-3C:Breakthrough in Launch Technology for Deep Space Exploration

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Aerospace China 2010年第4期11卷 12-15页

作者： li dan hu Wei Wei Yuanming Systems Engineering Division China Academy of Launch Vehicle Technology

One of the main objectives of the Chang'e 2 mission was to demonstrate the direct Earth- moon transfer orbit launch technol- *** October 1,2010,a LM-3C launch vehicle sent the Chang'e 2 into its preset orbit accuratel... 详细信息

关键词：深空探测技术发射技术长征 C型转移轨道运载火箭

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基于递归测度主成分分析的振动信号非平稳评价

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中国科学：技术科学 2017年第7期47卷 675-684页

作者：杨栋任伟新李丹胡异丁合肥工业大学土木与水利工程学院合肥230009 湘潭大学土木工程与力学学院湘潭411105 五邑大学信息工程学院江门529020

在递归损失量和递归趋势两个非平稳测度的基础上,结合主成分分析方法,提出一套实用的振动信号非平稳评价方法.首先,在一定观测时间尺度的基础上,分别建立由递归损失量和递归趋势两个测度组成的样本向量;依据工作平稳性的定义,将两个向... 详细信息

在递归损失量和递归趋势两个非平稳测度的基础上,结合主成分分析方法,提出一套实用的振动信号非平稳评价方法.首先,在一定观测时间尺度的基础上,分别建立由递归损失量和递归趋势两个测度组成的样本向量;依据工作平稳性的定义,将两个向量组装成多变量的样本矩阵,对其进行主成分分析,取第一主成分作为振动信号非平稳性综合评价的对象,构造非平稳性的统计模式差异指标,并通过几种典型的数值模拟非平稳信号的评价结果验证指标的可靠性.最后,将此方法应用于桥梁结构环境振动信号分析,以得到更加客观的分析结果.

关键词：非平稳递归测度时间尺度主成分分析环境振动

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Increase in the variability of terrestrial carbon uptake in response to enhanced future ENSO modulation

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Atmospheric and Oceanic Science Letters 2025年第1期18卷 32-38页

作者： Younong li li dan Jing Peng Qidong Yang Fuqiang Yang Key Laboratory of Regional Climate-Environment for Temperate East Asia Institute of Atmospheric PhysicsChinese Academy of SciencesBeijingChina Key Laboratory of Atmospheric Environment and Processes in the Boundary Layer over the Low-Latitude Plateau Region Department of Atmospheric ScienceYunnan UniversityKunmingChina

El Niño-Southern Oscillation(ENSO)is a major driver of climate change in middle and low latitudes and thus strongly influences the terrestrial carbon cycle through land-air *** the ENSO modulation and carbon flux variability are projected to increase in the future,but their connection still needs further *** investigate the impact of future ENSO modulation on carbon flux variability,this study used 10 CMIP6 earth system models to analyze ENSO modulation and carbon flux variability in middle and low latitudes,and their relationship,under different scenarios simulated by CMIP6 *** results show a high consistency in the simulations,with both ENSO modulation and carbon flux variability showing an increasing trend in the *** higher the emissions scenario,especially SSP5-8.5 compared to SSP2-4.5,the greater the increase in *** flux variability in the middle and low latitudes under SSP2-4.5 increases by 30.9%compared to historical levels during 1951-2000,while under SSP5-8.5 it increases by 58.2%.Further analysis suggests that ENSO influences mid-and low-latitude carbon flux variability primarily through *** occurrence may potentially be attributed to the increased responsiveness of gross primary productivity towards regional temperature fluctuations,combined with the intensified influence of ENSO on land surface temperatures.

关键词： Carbon variability ENSO modulation CMIP6 models

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Return to work in young and middle-aged colorectal cancer survivors:Factors influencing self-efficacy,fear,resilience,and financial toxicity

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World Journal of Gastroenterology 2025年第1期31卷 79-92页

作者： dan hu Yue li hua Zhang lian-lian Wang Wen-Wen liu Xin Yang Ming-Zhao Xiao Hao-ling Zhang Juan li Department of Nursing The First Affiliated Hospital of Chongqing Medical UniversityChongqing 400016China Department of Nursing National Clinical Research Center for Child Health and DisordersMinistry of Education Key Laboratory of Child Development and DisordersChildren’s Hospital of Chongqing Medical UniversityChongqing 400014China Department of Gastrointestinal Surgery The First Affiliated Hospital of Chongqing Medical UniversityChongqing 400016China Department of Biomedical Science Advanced Medical and Dental InstituteUniversity Sains MalaysiaPenang 13200Malaysia Academic Affairs Office The First Affiliated Hospital of Chongqing Medical UniversityChongqing 400016China

BACKGROUND Return to work(RTW)serves as an indication for young and middle-aged colorectal cancer(CRC)survivors to resume their normal social ***,these survivors encounter significant challenges during their RTW ***,scientific research is necessary to explore the barriers and facilitating factors of returning to work for young and middle-aged CRC *** To examine the current RTW status among young and middle-aged CRC survivors and to analyze the impact of RTW self-efficacy(RTW-SE),fear of progression(FoP),eHealth literacy(eHL),family resilience(FR),and financial toxicity(FT)on their RTW *** A cross-sectional investigation was adopted in this *** September 2022 to February 2023,a total of 209 participants were recruited through a convenience sampling method from the gastrointestinal surgery department of a class A tertiary hospital in *** investigation utilized a general information questionnaire alongside scales assessing RTW-SE,FoP,eHL,FR,and *** analyze the factors that influence RTW outcomes among young and middle-aged CRC survivors,Cox regression modeling and Kaplan-Meier survival analysis were *** A total of 43.54%of the participants successfully returned to work,with an average RTW time of 100 *** regression univariate analysis revealed that RTW-SE,FoP,eHL,FR,and FT were significantly different between the non-RTW and RTW groups(Plitating their timely reintegration into mainstream society.

关键词： Return to work Colorectal neoplasms Return-to-work self-efficacy Fear of progression Family resilience Financial toxicity

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Photo‑Energized MoS_(2)/CNT Cathode for High‑Performance li–CO_(2)Batteries in a Wide‑Temperature Range

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Nano-Micro Letters 2025年第1期17卷 160-175页

作者： Tingsong hu Wenyi lian Kang hu Qiuju li Xueliang Cui Tengyu Yao Laifa Shen Jiangsu Key Laboratory of Materials and Technologies for Energy Storage College of Materials Science and TechnologyNanjing University of Aeronautics and AstronauticsNanjing 210016People’s Republic of China

li–CO_(2) batteries are considered promising energy storage systems in extreme environments such as Mars;however,severe performance degradation will occur at a subzero temperature owning to the sluggish reaction ***,a photo-energized strategy adopting sustainable solar energy in wide working temperature range li–CO_(2) battery was achieved with a binder-free MoS_(2)/carbon nanotube(CNT)photo-electrode as *** unique layered structure and excellent photoelectric properties of MoS_(2) facilitate the abundant generation and rapid transfer of photo-excited carriers,which accelerate the CO_(2) reduction and li_(2)CO_(3) decomposition upon *** illuminated battery at room temperature exhibited high discharge voltage of 2.95 V and mitigated charge voltage of 3.27 V,attaining superior energy efficiency of 90.2%and excellent cycling stability of over 120 *** at an extremely low temperature of−30℃,the battery with same electrolyte can still deliver a small polarization of 0.45 V by the photoelectric and photothermal synergistic mechanism of MoS_(2)/CNT *** work demonstrates the promising potential of the photo-energized wide working temperature range li–CO_(2) battery in addressing the obstacle of charge overpotential and energy efficiency.

关键词： li-CO_(2)batteries Photo-energized Wide operation-temperature Kinetics MoS_(2)

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Unlocking the stable interface in aqueous zinc-ion battery with multifunctional xylose-based electrolyte additives

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Journal of Energy Chemistry 2025年第1期100卷 770-778页

作者： Xiaoqin li Jian Xiang Lu Qiu Xiaohan Chen Yinkun Zhao Yujue Wang Qu Yue Taotao Gao Wenlong liu dan Xiao Zhaoyu Jin Panpan li Institute for Advanced Study Chengdu University School of Mechanical Engineering Chengdu University College of Food and Biological Engineering Chengdu University Institute of Fundamental and Frontier Sciences University of Electronic Science and Technology of China College of Materials Science and Engineering Sichuan University

The growth of dendrites and the side reactions occurring at the Zn anode pose significant challenges to the commercialization of aqueous Zn-ion batteries（AZIBs）. These challenges arise from the inherent conflict between mass transfer and electrochemical kinetics. In this study, we propose the use of a multifunctional electrolyte additive based on the xylose（Xylo） molecule to address these issues by modulating the solvation structure and electrode/electrolyte interface, thereby stabilizing the Zn anode. The introduction of the additive alters the solvation structure, creating steric hindrance that impedes charge transfer and then reduces electrochemical kinetics. Furthermore, in-situ analyses demonstrate that the reconstructed electrode/electrolyte interface facilitates stable and rapid Zn2+ion migration and suppresses corrosion and hydrogen evolution reactions. As a result, symmetric cells incorporating the Xylo additive exhibit significantly enhanced reversibility during the Zn plating/stripping process, with an impressively long lifespan of up to 1986 h, compared to cells using pure ZnSO4electrolyte. When combined with a polyaniline cathode, the full cells demonstrate improved capacity and long-term cyclic stability. This work offers an effective direction for improving the stability of Zn anode via electrolyte design, as well as highperformance AZIBs.

关键词： Solvation

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