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In vivo bioluminescence imaging of hyperglycemia exacerbating stem cells on choroidal neovascularization in mice

International Journal of Ophthalmology(English edition) 2016年第4期9卷 519-527页

作者： Xiang Gao Yu Wang Hui-Yuan Hou Yang Lyu Hai-Yan Wang li-bo yao Jian Zhang Feng Cao Yu-Sheng Wang Department of Ophthalmology Xijing Hospital Eye Institute of Chinese PLA Fourth Military Medical University State Key Laboratory of Cancer Biology Department of Biochemistry and Molecular Biology Fourth Military Medical University Department of Cardiology Xijing Hospital Fourth Military Medical University

AIM： To investigate the influence of hyperglycemia on the severity of choroidal neovascularization（CNV）,especially the involvement of bone marrow-derived cells（BMCs） and underlying mechanisms.·METHODS： BMCs from firefly luciferase（Fluc）/green fluorescent protein（GFP） double transgenic mice were transplanted into C57BL/6J wide-type mice. The recipient mice were injected intraperitoneally with streptozotocin（STZ） daily for 5 consecutive days to induce diabetes mellitus（DM）, followed by CNV laser *** BMCs recruitment in CNV exposed to hyperglycemia was firstly examined in Fluc/GFP chimeric mice by in vivo optical bioluminescence imaging（Bli） and in vitro Fluc assays. The CNV severity was evaluated by H＆E staining and choroidal flatmount. The expression of vascular endothelial growth factor（VEGF） and stromal cell derived factor-1（SDF-1） was detected by Western blot.·RESULTS： Bli showed that the BMCs exerted dynamic effects in CNV model in Fluc/GFP chimeric mice exposed to hyperglycemia. The signal intensity of transplanted Fluc＋GFP＋BMCs in the DM chimeric mice was significantly higher than that in the control chimeric mice with CNV induction at days 5, 7, 14 and 21（121861.67 ±9948.81 vs 144998.33 ±13787.13 photons/second/cm2/sr for control and DM mice, P5d〈0.05; 178791.67±30350.8 vs240166.67 ±22605.3, P7d〈0.05; 124176.67 ±16253.52 vs196376.67 ±18556.79, P14d〈0.05; 97951.60 ±10343.09 vs119510.00 ±14383.76, P21d〈0.05）, which was consistent with in vitro Fluc assay at day 7 [relative light units of Fluc（RLU1）], 215.00±52.05 vs 707.33±88.65, P 〈0.05; RLU1/relative light units of renilla luciferase（RLU2）, 0.90 ±0.17 vs 1.83 ±0.17, P 〈0.05]. The CNVs in the DM mice were wider than those in the control group at days 5, 7, 14 and21（147.83±17.36 vs 220.33±20.17 μm, P5d〈0.05; 212.17 ±24.63 vs 326.83 ±19.49, P7d〈0.05; 163.17 ±18.24 vs265.17 ±20.55, P14d〈0.05; 132.00 ±10.88 vs 205.33 ±12.98,P21d〈0.05）. The average area of CNV in the DM group was larger at 7d（20688.

关键词： hyperglycemia choroidal neovascularization bone marrow-derived cells molecular imaging in vivo optical bioluminescence imaging

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武孝铁路引入汉口站方案研究

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铁道运输与经济 2010年第9期32卷 25-28页

作者：李耀博中铁第四勘察设计院集团有限公司线路站场设计研究处湖北武汉430063

武汉—孝感铁路是武汉城市圈铁路交通网络的重要组成部分,根据武汉枢纽内既有车流情况及武汉城市圈铁路交通网络建成后的运输模式,提出武孝铁路原则上以不过江引入枢纽为宜,即引入既有汉口站。在此基础上,研究武孝铁路引入汉口站的3个方... 详细信息

武汉—孝感铁路是武汉城市圈铁路交通网络的重要组成部分,根据武汉枢纽内既有车流情况及武汉城市圈铁路交通网络建成后的运输模式,提出武孝铁路原则上以不过江引入枢纽为宜,即引入既有汉口站。在此基础上,研究武孝铁路引入汉口站的3个方案,结合汉口站既有规模和疏解区格局,从行车组织、工程投资及运营成本、城市现状及规划、相邻拟建工程等方面进行分析,建议采用武孝铁路自汉口站东端咽喉线路别引入方案。

关键词：铁路武汉枢纽汉口站新线引入

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Nanostructured ZnO/BiVO_(4)I-scheme heterojunctions for piezocatalytic degradation of organic dyes via harvesting ultrasonic vibration energy

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International Journal of Minerals,Metallurgy and Materials 2025年第2期32卷 488-497页

作者： Yiling li Xiaoyao Yu Yingjie Zhou yao lin Ying Wu Key Laboratory of the Ministry of Education for Advanced Catalysis Materials College of Chemistry and Materials ScienceZhejiang Normal UniversityJinhua 321004China

BiVO_(4)porous spheres modified by ZnO were designed and synthesized using a facile two-step *** resulting ZnO/BiVO_(4)composite catalysts have shown remarkable efficiency as piezoelectric catalysts for degrading Rhodamine B(RhB)unde mechanical vibrations,they exhibit superior activity compared to pure *** 40wt%ZnO/BiVO_(4)heterojunction composite displayed the highest activity,along with good stability and *** enhanced piezoelectric catalytic activity can be attributed to the form ation of an I-scheme heterojunction structure,which can effectively inhibit the electron-hole ***,hole(h+)and superoxide radical(·O_(2)^(-))are proved to be the primary active ***,ZnO/BiVO_(4)stands as an efficient and stable piezoelectric catalyst with broad potential application in the field of environmental water pollution treatment.

关键词： piezoelectric catalytic heterojunction dye degradation ultrasonic vibration

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N-Myc Downstream-regulated Gene 2 (NDRG2) is Involved in p53-mediated Apoptosis

N-Myc Downstream-regulated Gene 2 (NDRG2) is Involved in p53...

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2008年全国生物化学与分子生物学学术大会

作者： li-bo yao liu Na li Xia li-feng Wang (Department of Biochemistry and Molecular Biology,The Fourth Military Medical University,Xian 710032,China)

The tumor suppressor,p53,is a transcription factor which can modulate the transcription of a number of target genes that are involved in cell-cycle arrest and ***,the apoptotic pathway mediated by p53 is not fully und...

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Fusion Expression of DDR2 Extracellular Domain in Insect Cells and its Purification and Function Characterization

Fusion Expression of DDR2 Extracellular Domain in Insect Cel...

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2008年全国生物化学与分子生物学学术大会

作者： Wei Zhang li-bo yao (Department of Microbiology,The Fourth Military Medical University,Xian 710032,P.R.China)

Discoidin domain receptor 2 (DDR2) is a kind of protein tyrosine kinases associated with cell proliferation and tumor metastasis,and collagen,a ligand for DDR2,up-regulates matrix metalloproteinase 1 (MMP-1) and MMP-2 expression in extracellular matrix (ECM).To investigate the role of DDR2 in cartilage destruction in

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Stress Field Analysis of Carburized Specimen and Its Numerical Simulation

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Journal of Shanghai Jiaotong university(Science) 2000年第1期5卷 161-164页

作者： Qingxiang Yang, Yukui Gao, Yanli li, bo liao, Mei yao 1.School of Materials Science and Chemical Engineering, Yanshan University, Qinhuangdao 066004, China

Residual stress field of carburized specimens was determined experimentally and simulated by using a finite element method (FEM). The experimental results show that the compressive residual stress is formed in carburized layer of the specimen. The peak value of compressive stress appears inside of carburized layer. The calculated results are tallied with the measured ones quite well, which proves the FEM model used for simulation is correct. According to this model, the stress field of carburized specimen during quenching process was also analyzed in this paper.

关键词： Carburizing Residual Stress Field, Stress Field Simulation Martensite Transformation

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内蒙古典型草原季节性冻土区土壤剖面CO2、N2O特征

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环境科学 2018年第5期39卷 2330-2338页

作者：李晋波姚楠李秀赵英张阿凤兰志龙范庭西北农林科技大学资源环境学院农业部西北植物营养与农业环境重点实验室杨凌712100

为了评估放牧对内蒙古典型草原季节性冻土区温室气体产生机制的影响,通过气体原位采集系统对该区土壤剖面不同土层N_2O、CO_2浓度动态变化进行了为期1a的田间原位监测.共设3个处理:1979年以来规划的禁牧(UG79)、1999年以来规划的禁牧(UG... 详细信息

为了评估放牧对内蒙古典型草原季节性冻土区温室气体产生机制的影响,通过气体原位采集系统对该区土壤剖面不同土层N_2O、CO_2浓度动态变化进行了为期1a的田间原位监测.共设3个处理:1979年以来规划的禁牧(UG79)、1999年以来规划的禁牧(UG99)和持续放牧(CG)小区.结果表明:土壤剖面N_2O、CO_2浓度具有明显的时空分布特征:(1)3个处理不同时期土壤剖面CO_2平均浓度均表现为:生长期>冻融期>冻结期,且生长期CO_2浓度要远大于冻融期和冻结期;UG79土壤剖面CO_2浓度最高,CG最低;不同土层间CO_2浓度有所差异,具体表现为UG79、UG99:20 cm≥50 cm≥35 cm≥10 cm≥5 cm;CG:50 cm≥35 cm≥20 cm≥10 cm≥5 cm;(2)土壤剖面N_2O浓度时空变化特征则与CO_2不同,UG79、UG99呈现出"单峰型"变化规律,CG为"双峰型"变化规律,即虽然CG在生长期N_2O也有微弱的释放,但3个处理土壤剖面N_2O浓度均在土壤冻融期内急剧增加,其中CG处理土壤剖面N_2O平均浓度最高,与UG79、UG99差异显著(P<0.05);不同土层土壤剖面N_2O浓度表现为UG79:20 cm≥50 cm≥35 cm≥10 cm≥5 cm;CG:50 cm≥35 cm≥20 cm≥10 cm≥5 cm;UG99:35 cm≥50 cm≥20 cm≥10 cm≥5 cm.研究得出放牧可以使该区土壤剖面CO_2浓度降低、N_2O浓度升高,这为我国草原季节性冻土区温室气体的准确评估提供了重要的理论支持.

关键词：冻融作用典型草原土壤剖面温室气体放牧

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SY-1井页岩取心钻井工艺

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内蒙古石油化工 2018年第3期44卷 60-63页

作者：李伯尧中国石油化工股份有限公司华东油气分公司江苏南京210031

SY-1井是部署在川东南构造东胜背斜的第一口页岩气探井,本井的取心作业承担着获取该区块页岩气地质评价参数,落实页岩气勘探潜力的重要任务。通过分析该井上部地层易漏失、井眼轨迹平滑度差和页岩易碎、易堵心等施工难点,对取心工具及... 详细信息

SY-1井是部署在川东南构造东胜背斜的第一口页岩气探井,本井的取心作业承担着获取该区块页岩气地质评价参数,落实页岩气勘探潜力的重要任务。通过分析该井上部地层易漏失、井眼轨迹平滑度差和页岩易碎、易堵心等施工难点,对取心工具及取心钻头的选型、取心参数的优选和取心过程复杂情况的预防措施进行了研究,制定了合理的取心关键技术措施,确保了取心施工顺利完成。该井累计取心9次,取心进尺151.7m,平均岩心收获率100%,为该区块页岩取心作业积累了宝贵经验。

关键词：页岩取心钻井技术措施

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Expression and Subcellular Localization of a Novel Gene Bm-X of Silk Worm,bombyx mori

Expression and Subcellular Localization of a Novel Gene Bm-X...

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2008年全国生物化学与分子生物学学术大会

作者： Zheng-bing Lǜ bo li yao-zhou Zhang (Institute of Biochemistry,College of life science,Zhejiang Sci-Tec University,Hangzhou 310018,China)

According to the large-scale sequencing of cDNA library from silkworm pupae,the cDNA of a novel gene with blank research background was identified and temporary named *** length of this cDNA is 778 bp. Through bioinfo...

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The gut-eye axis:from brain neurodegenerative diseases to age-related macular degeneration

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Neural Regeneration Research 2025年第10期20卷 2741-2757页

作者： Qianzi Jin Suyu Wang Yujia yao Qin Jiang Keran li Department of Ophthalmology The Affiliated Eye Hospital of Nanjing Medical UniversityNanjingJiangsu ProvinceChina The Fourth School of Clinical Medicine Nanjing Medical UniversityNanjingJiangsu ProvinceChina

Age-related macular degeneration is a serious neurodegenerative disease of the retina that significantly impacts ***,the specific pathogenesis remains unclear,and effective early treatment options are consequently *** microbiome is defined as a large ecosystem of microorganisms living within and coexisting with a *** intestinal microbiome undergoes dynamic changes owing to age,diet,genetics,and other *** dysregulation of the intestinal flora can disrupt the microecological balance,resulting in immunological and metabolic dysfunction in the host,and affecting the development of many *** recent decades,significant evidence has indicated that the intestinal flora also influences systems outside of the digestive tract,including the ***,several studies have demonstrated the critical role of the gut-brain axis in the development of brain neurodegenerative diseases,including Alzheimer’s disease and Parkinson’s ***,the role of the“gut-eye axis”has been confirmed to play a role in the pathogenesis of many ocular ***,age-related macular degeneration and many brain neurodegenerative diseases have been shown to share several risk factors and to exhibit comparable *** such,the intestinal flora may play an important role in age-related macular *** the above context,the present review aims to clarify the gut-brain and gut-eye connections,assess the effect of intestinal flora and metabolites on age-related macular degeneration,and identify potential diagnostic markers and therapeutic ***,direct research on the role of intestinal flora in age-related macular degeneration is still relatively limited,while studies focusing solely on intestinal flora are insufficient to fully elucidate its functional role in age-related macular ***-on-a-chip technology has shown promise in clarifying the gut-eye interactions,while integrating analysis of the inte

关键词： age-related macular degeneration biological agents blinding eye disease dietary nutrition fecal microbial transplantation gut-eye axis intestinal flora metabolite microecology neurodegenerative disease

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