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Effect of the gene silencing of phosphorus transporters on phosphorus absorption across primary cultured duodenal epithelial cell monolayers of chick embryos

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Journal of Integrative Agriculture 2022年第7期21卷 2076-2085页

作者： li ting-ting LU Na SHAO Yu-xin ZHANG li-yang LU lin liU Zong-ping LUO Xu-gang liAO Xiu-dong Poultry Mineral Nutrition Laboratory College of Animal Science and TechnologyYangzhou UniversityYangzhou 225009P.R.China Mineral Nutrition Research Division Institute of Animal SciencesChinese Academy of Agricultural SciencesBeijing 100193P.R.China College of Veterinary Medicine Yangzhou UniversityYangzhou 225009P.R.China

The aim of the study was to investigate whether phosphorus(P) transporters, type IIb sodium-dependent phosphate cotransporter(NaP-IIb) and inorganic phosphate transporter 2(PiT2), were directly involved in P absorption across primary cultured duodenal epithelial cell monolayers of chick embryos. The siRNAs against NaP-IIb or PiT2 were designed, synthesized and transfected into primary cultured duodenal epithelial cells of chick embryos. Then, the inhibitory efficiency of siRNAs against NaP-IIb or PiT2 was analyzed, and the most efficacious siRNAs were selected to be used for subsequent P absorption experiments. Briefly, primary cultured duodenal epithelial cells of chick embryos were transfected with either NaP-IIb or PiT2 siRNAs and grown in confluent monolayers on transwell plates. The untransfected or transfected cell monolayers were then incubated in an uptake medium containing 0 or 0.25 mmol L^(–1) of P as KH_(2) PO_(4) to measure the P absorption across duodenal epithelial cell monolayers. The results showed that among the siRNAs designed, si-1372 and si-890 were demonstrated to be the most effective in inhibiting the NaPIIb and PiT2 expressions, respectively. Supplemental P increased(P=0.065) the protein abundance of PiT2 and enhanced(Plial cell of chick embryos. Furthermore, NaPIIb silencing decreased(P=0.07) P absorption across duodenal epithelial cell monolayers, while PiT2 silencing had no effect(P=0.345). It is concluded that the NaP-IIb, but not PiT2, might be directly involved in the P absorption of chick duodenal epithelial cells.

关键词： broiler phosphorus transporter phosphorus absorption primary cultured duodenal epithelial cell

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The Role of Underlying Boundary Forcing in Shaping the Recent Decadal Change of Persistent Anomalous Activity over the Ural Mountains

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Advances in Atmospheric Sciences 2024年第8期41卷 1496-1510,1661-1667页

作者： ting LEI Shuanglin li Climate Change Research Center Institute of Atmospheric PhysicsChinese Academy of SciencesBeijing 100029China Beijing Meteorological Service Center Beijing 100089China University of Chinese Academy of Sciences Beijing 100049China Department of Atmospheric Science China University of GeosciencesWuhan 430074China

Observational analyses demonstrate that the Ural persistent positive height anomaly event(PAE) experienced a decadal increase around the year 2000, exhibiting a southward displacement afterward. These decadal variations are related to a large-scale circulation shift over the Eurasian Continent. The effects of underlying sea ice and sea surface temperature(SST) anomalies on the Ural PAE and the related atmospheric circulation were explored by Atmospheric Model Intercomparison Project(AMIP) experiments from the Coupled Model Intercomparison Project Phase 6 and by sensitivity experiments using the Atmospheric General Circulation Model(AGCM). The AMIP experiment results suggest that the underlying sea ice and SST anomalies play important roles. The individual contributions of sea ice loss in the Barents-Kara Seas and the SST anomalies linked to the phase transition of the Pacific Decadal Oscillation(PDO) and Atlantic Multidecadal Oscillation(AMO) are further investigated by AGCM sensitivity experiments isolating the respective *** sea ice decline in Barents-Kara Seas triggers an atmospheric wave train over the Eurasian mid-to-high latitudes with positive anomalies over the Urals, favoring the occurrence of Ural PAEs. The shift in the PDO to its negative phase triggers a wave train propagating downstream from the North Pacific. One positive anomaly lobe of the wave train is located over the Ural Mountains and increases the PAE there. The negative-to-positive transition of the AMO phase since the late-1990s causes positive 500-h Pa height anomalies south of the Ural Mountains, which promote a southward shift of Ural PAE.

关键词： Ural persistent anomaly Pacific decadal oscillation Atlantic multidecadal oscillation sea ice loss in Barents-Kara Seas

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Identification of a major QTL for flag leaf glaucousness using a high-density SNP marker genetic map in hexaploid wheat

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Journal of Integrative Agriculture 2017年第2期16卷 445-453页

作者： li Chun-lian li ting-ting liU Tian-xiang SUN Zhong-pei BAI Gui-hua JIN Feng WANG Yong WANG Zhong-hua College of Agronomy Northwest A &F University Yangling 712100 P.R. China Agronomy Department Kansas State University Manhattan KS 66506 USA China Golden Marker (Beijing) Biotech Co. Ltd. Beijing 102206 P.R.China

Cuticular wax plays an important role in protecting land plant against biotic and abiotic stresses. Cuticular wax production on plant surface is often visualized by a characteristic glaucous appearance. This study identified quantitative trait loci （QTLs） for wheat （Triticum aestivum L.） flag leaf glaucousness （FLG） using a high-density genetic linkage map developed from a recombinant inbred line （RIL） population derived from the cross HeynexLakin by single-seed descent. The map consisted of 2 068 single nucleotide polymorphism （SNP） markers and 157 simple sequence repeat （SSR） markers on all 21 wheat chromosomes and covered a genetic distance of 2 381.19 cM, with an average marker interval of 1.07 cM. Two additive QTLs for FLG were identified on chromosomes 3AL and 2DS with the increasing FLG allele contributed from Lakin. The major QTL on 3AL, ***-3AL, explained 17.5-37.8% of the phenotypic variation in different environments. ***-3AL was located in a 4.4-cM interval on chromosome 3AL that was flanked by two markers IWA1831 and IWA8374. Another QTL for FLG on 2DS, designated as ***-2DS which was identified only in Yangling in 2014 （YL14）, was flanked by IWA1939 and Xgwm261 and accounted for 11.3% of the phenotypic variation for FLG. ***- gr-3AL and ***-2DS showed Additive×Environment （AE） interactions, explaining 3.5 and 4.4% of the phenotypic variance, respectively. Our results indicated that different genes/QTLs may contribute different scores of FLG in a cultivar and that the environment may play a role in FLG.

关键词： wheat quantitative trait locus (QTL) flag leaf glaucousness single nucleotide polymorphism QTL×Environment interactions

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Molecular Chaperone-Dependent Polymer Translocation through Nanopores: The Effects of Chaperone Concentration and Chaperone-Polymer Interaction

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Chinese Journal of Polymer Science 2024年第1期42卷 125-132,I0011页

作者： Chang-Sheng Zuo Kang Wang li-Zhen Sun ting-ting Sun Department of Physics Zhejiang University of Science and TechnologyHangzhou 310023China Department of Applied Physics Zhejiang University of TechnologyHangzhou 310014China

The polymer translocation through a nanopore from a donor space(or named cis side) to a receiver space(trans side) in the chaperone-induced crowded environment has attracted increasing attention in recent years due to its significance in biological systems and technological applications. In this work, we mainly focus on the effects of chaperone concentration and chaperone-polymer interaction on the polymer translocation. By assuming the polymer translocation to be a quasi-equilibrium process, the free energy F of the polymer can be estimated by Rosenbluth-Rosenbluth method and then the translocation time τ can be calculated by Fokker-Plank equation based on the obtained free energy landscape. Our calculation results show that the translocation time can be controlled by independently tuning the chaperone concentration and chaperone-polymer interaction at the cis side or the trans side. There exists a critical chaperone-polymer attraction ε~*=-0.2 at which the volume exclusion and interaction effects of the chaperone can balance each other. Additionally, we also find that at large chaperone-polymer attraction, the translocation time is mainly governed by the diffusion coefficient of the polymer.

关键词： Polymer translocation Rosenbluth-Rosenbluth method Fokker-Plank equation Free energy landscapes

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Improved ACE and ICF low complexity joint algorithm to reduce PAPR of CO-OFDM system

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Optoelectronics Letters 2021年第4期17卷 209-214页

作者： li ting-ting TONG Zheng-rong ZHANG Wei-hua liU-Ye Engineering Research Center of Optoelectronic Devices and Communication Technology Minislty of EducationTianjin Key Laboratory of Film Electronic and Communication DevicesSchool of Electrical and Electronic EngineeringTianjin University of TechnologyTianjin 300384China

Active constellation expansion(ACE) and iterative clipping and filtering(ICF) are simple and effective techniques for reducing the peak-to-average ratio(PAPR) in coherent optical orthogonal frequency division multiplexing(CO-OFDM) systems, but effective PAPR suppression requires a lot of iterations. To overcome this shortcoming, a joint algorithm based on improved active constellation expansion(IACE) and ICF(IACE-ICF) is proposed. The simulation results show that at the complementary cumulative distribution function(CCDF) of 10-4, the PAPR of IACE-ICF(G=4, iter=4) algorithm is optimized by 1.507 d B, 1.13 d B and 0.204 d B compared with that of the IACE, ICF(iter=4) and ICF-IACE(G=4, iter=4) algorithms, respectively. Meanwhile, when the bit error rate(BER) is 10-3, the optical signal to noise ratio(OSNR) of the proposed scheme is optimized by 2.04 d B, 1.75 d B and 1.4 d B compared with that of clipping, ICF(iter=4) and ICF-IACE(G=4, iter=4) algorithms, respectively. On the other hand, the proposed scheme can reduce the number of complex multiplications by 14.29% and complex additions by 28.57% compared with the ICF(iter=14) scheme.

关键词： algorithm expansion scheme

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食品减盐措施和氯化钠检测技术研究进展

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食品安全质量检测学报 2024年第10期15卷 144-154页

作者：李婷婷王蓉刘盼任兴权曾文锦孙姗姗酒泉市食品检验检测中心(酒泉市质量检验检测中心) 酒泉735000 中国食品药品检定研究院/国家市场监管重点实验室(食品质量与安全) 北京100050

食盐在人们的日常饮食中不可或缺,也在食品工业中发挥重要作用。一系列监测数据表明,人们日常食盐摄入量远高于世界卫生组织建议的5 g/d。高盐饮食带来的健康风险在世界范围内得到广泛关注,降低居民每日食盐摄入量已成为共识。随着各国... 详细信息

食盐在人们的日常饮食中不可或缺,也在食品工业中发挥重要作用。一系列监测数据表明,人们日常食盐摄入量远高于世界卫生组织建议的5 g/d。高盐饮食带来的健康风险在世界范围内得到广泛关注,降低居民每日食盐摄入量已成为共识。随着各国政府和世界卫生组织的一系列措施干预,食品减盐已取得初步效果。本文介绍了包含直接减少食盐用量、优化食盐物理结构和改变食盐分布形态、添加咸味增强剂、使用非钠盐替代、多感官协同作用、改进加工工艺及多种措施协同作用等方法在内的减盐措施,并对它们的减盐效果和应用前景进行阐述,分别从以钠基计和以氯基计两个方面评述了13种食品中氯化钠含量的检测方法,主要介绍了它们的测试原理、前处理方法改进和应用范围,以期为食品减盐措施和氯化钠检测技术的深入研究提供有价值的参考。

关键词：减盐措施检测技术氯化钠

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基础理论与实践应用深度融合的生物化学教学——Henderson-Hasselbalch方程

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化学教育（中英文） 2024年第14期45卷 98-104页

作者：海华何明磊那立艳金黎明高明波门磊李兴泰冮洁朴永哲金华李春斌权春善李婷婷陈会英王艳颖大连民族大学生命科学学院辽宁大连116600

以生物化学课程基础理论教学中Henderson-Hasselbalch方程的教学为例,对其教学内容和教学方法进行改革,深入挖掘该方程在多种实践及工程中的应用,尝试在教学中扩展其实践与应用的讲解。结果表明,通过对该方程实践与应用深入细致地讲解,... 详细信息

以生物化学课程基础理论教学中Henderson-Hasselbalch方程的教学为例,对其教学内容和教学方法进行改革,深入挖掘该方程在多种实践及工程中的应用,尝试在教学中扩展其实践与应用的讲解。结果表明,通过对该方程实践与应用深入细致地讲解,能使学生更好地理解和掌握该方程,有效夯实学生对该理论知识的理解和掌握,同时能提高学生应用该方程思考实际问题并做出理性判断的能力。

关键词： Henderson-Hasselbalch方程生物化学教学改革实践与应用

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铝合金车轮涂装前处理工序的节能减排

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中国涂料 2024年第2期39卷 62-65页

作者：韩跃刘海峰李婷婷杨申马玉松郭博程立中车轮集团河北保定071000

为提高水资源利用效率,达到节能降耗的目的,对铝合金车轮前处理进行中水回用及逆向补水可行性研究。优化补水管路,严格监控水洗槽电导率及末道水洗菌群污染程度;对铝合金车轮涂膜性能(附着力、硬度、耐碎石冲击、耐CASS试验、耐盐雾性... 详细信息

为提高水资源利用效率,达到节能降耗的目的,对铝合金车轮前处理进行中水回用及逆向补水可行性研究。优化补水管路,严格监控水洗槽电导率及末道水洗菌群污染程度;对铝合金车轮涂膜性能(附着力、硬度、耐碎石冲击、耐CASS试验、耐盐雾性、耐丝状腐蚀性、耐水性、耐湿性及耐洗涤剂)进行测试。结果标明:涂装前处理中水回用及逆向补水工艺稳定,水洗槽电导率及末道水洗含菌量均满足前处理工艺指标要求,涂膜性能合格。

关键词：铝合金轮毂节能降耗中水回用逆向补水含菌量涂膜性能

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A rapid, simple, and sensitive immunoagglutination assay with silica nanoparticles for serotype identification of Pseudomonas aeruginosa

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Journal of Integrative Agriculture 2019年第8期18卷 1936-1943页

作者： DONG Xiu-mei TAO Jing li ting-ting ZHANG Ping ZHU Yan TANG Yu SU Rui-hong SHI Dong-fang College of Veterinary Medicine Northeast Agricultural University Harbin 150030 P.R.China Heilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical Development College of Veterinary MedicineNortheast Agricultural University Harbin 150030 P.R.China

An agglutination test based on colored silica nanoparticles(colored SiNps) was established to detect serotypes of Pseudomonas aeruginosa. Monodisperse colored SiNps were used as agglutination test carriers. The colored SiNps were prepared through reverse microemulsion with reactive dyes, sensitized with 11 kinds of mono-specific antibodies against P. aeruginosa, and denoted as IgG-colored SiNps. Eleven kinds of IgG-colored SiNps were individually mixed with P. aeruginosa on a glass *** serotypes of P. aeruginosa could be identified by agglutination test with evident agglutination. The P. aeruginosa could be detected in a range from 3.6×10^5 to 3.6×10^12 cfu mL^–1. This new agglutination test was confirmed to be a specific,sensitive, fast, easy-to-perform, and cost-efficient tool for the routine diagnosis of P. aeruginosa.

关键词： Pseudomonas aeruginosa colored silica nanoparticles agglutination test serotype detection

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Reshaping force for deformed casing repairing with hydraulic rolling reshaper and its influencing factors

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Petroleum Science 2024年第4期21卷 2745-2757页

作者： Hong-Fei li Min Luo ting-ting Xu Qiao-Zhen li Cong-Jian Huang School of Mechanical Science and Engineering Northeast Petroleum University

Hydraulic rolling reshaper is an advanced reshaping tool to solve the problem of casing deformation,which has been widely used in recent years. When it is used for well repair operation, the reshaping force provided by ground devices is generally determined by experience. However, too large reshaping force may destroy the deformed casing, and too small reshaping force may also prolong the construction period and affect the repairing effect. In this paper, based on Hertz contact theory and elastic-plastic theory, combined with the process parameters of shaping, and considering the structural characteristics of the deformed casing and reshaper, we propose a mathematical model for calculating the reshaping force required for repairing deformed casing by hydraulic rolling reshaper. Meanwhile, the finite element model and numerical method of hydraulic rolling reshaper repairing deformed casing are established by using the finite element method, and the reliability of the mathematical model is verified by several examples. On this basis, the control variable method is used to investigate the influence of each parameter on the reshaping force, and the influence degree of each parameter is explored by orthogonal simulation test and Pearson correlation analysis. The research results not only provide an important theoretical basis for the prediction of reshaping force in on-site construction, but also provide a reference for the subsequent improvement of the shaping process.

关键词： Hydraulic rolling reshaper Reshaping force Deformed casing Casing reshaping Hertz contact theory

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