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Treatment of SMA type 1 infants using a single-dose AAV9-mediated gene therapy via intrathecal injection of GC101: An open-label, single-arm study

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中华医学杂志(英文版) 2024年第16期137卷 2024 Jul 9页

作者： Ma Xiuwei Zhuang Lu Ma Wenhao li Jun Wang Xiaodong li zhongqiu Jiang Xinyang Wang Yongxia Du Ying Zhang Yingqian He Fang Zhu Zhiming Du Shaopeng Xu Juan Gu Ruijie Zhang Yanping Zhang Shan li Ting Yang Xiao Zhang Sheng Zhu lina li Qiuping Dong Xiaoyan Wu Xiaobing Feng Zhichun Department of Neurodevelopment Faculty of Pediatrics The Seventh Medical Center of Chinese PLA General Hospital Beijing China National Engineering Laboratory for Birth Defects Prevention and Control of Key Technology Beijing China Research and Development Division Genecradle Therapeutics Inc. Beijing China Medical Division Genecradle Therapeutics Inc. Beijing China Department of Pharmacy Medical Supplies Center of Chinese PLA General Hospital Beijing China Beijing Key Laboratory of Pediatric Organ Failure Beijing China

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南京大学仙林校区流浪猫种群数量和繁殖调查

兽类学报 2024年第3期44卷 333-343页

作者：孙桃柱吴琼张振伟李忠秋南京大学生命科学学院动物行为与保护实验室南京210023

流浪猫已对全球生物多样性带来严重威胁,然而,中国对流浪猫的基础生物学研究却很少。2018—2022年,以南京大学仙林校区(约195 hm2)为研究区域,开展了基于个体识别的校园流浪猫种群及繁殖等基础生物学研究。通过连续20 d的样点调查,构建... 详细信息

流浪猫已对全球生物多样性带来严重威胁,然而,中国对流浪猫的基础生物学研究却很少。2018—2022年,以南京大学仙林校区(约195 hm2)为研究区域,开展了基于个体识别的校园流浪猫种群及繁殖等基础生物学研究。通过连续20 d的样点调查,构建了流浪猫的种群数量累积曲线,发现即便在熟知流浪猫种群分布的基础上,一天调查中流浪猫的发现率仅为51.22%(44.51%~53.66%),而累积6 d的重复调查,发现率可达到84.15%(81.10%~86.59%)。2018—2022年南京大学仙林校区流浪猫数量快速增长,从2018年的51只增加至2022年的220只。2021年3月至2022年3月每月6次调查,期间共记录到273只流浪猫,其中成年猫149只,幼猫124只,雌雄性比约为1∶1。在此期间流浪猫的一年留存率约为58.71%,且绝育流浪猫的留存率显著高于未绝育的流浪猫。流浪猫全年均可繁殖,但产仔高峰集中在3—6月。65窝幼猫中,有84只存活超过6个月,若家猫的平均产仔数为3~4只,则南京大学仙林校区的流浪猫幼仔6月龄成活率约为36.92%(32.31%~43.08%)。上述结果说明,低强度的样点调查可能低估了流浪猫的种群数量。同时,在人类提供庇佑的环境中,流浪猫的生存不受食物、住所以及疾病的限制,其生存率和繁殖成功率较高,高存活率和高繁殖力将为流浪猫种群数量的控制带来困难。

关键词：流浪猫种群数量种群动态繁殖参数

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企业社交媒体交流可见性对工作场所学习的影响机制

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中国人力资源开发 2024年第7期41卷 55-68页

作者：梁亮潘保臻李仲秋张雪东北林业大学经济管理学院哈尔滨150040 广州大学教育学院广州510006 东北农业大学经济管理学院哈尔滨150030 上海师范大学哲学与法政学院上海200233

数字化背景下企业社交媒体的广泛应用使得企业社交媒体交流可见性成为学术界关注的热点。学者围绕企业社交媒体交流可见性在工作场所的具体效用展开了实证研究,探讨了企业社交媒体交流可见性对员工建言行为、知识共享等主动行为结果的... 详细信息

数字化背景下企业社交媒体的广泛应用使得企业社交媒体交流可见性成为学术界关注的热点。学者围绕企业社交媒体交流可见性在工作场所的具体效用展开了实证研究,探讨了企业社交媒体交流可见性对员工建言行为、知识共享等主动行为结果的影响。然而,员工工作场所学习作为一种主动行为是否受企业社交媒体交流可见性影响尚不明确。本研究基于自我决定理论,引入和谐式工作激情和主动性人格变量,探讨企业社交媒体交流可见性与员工工作场所学习之间的关系,并利用来自中国北方一家服务业企业的287份有效问卷进行实证检验。研究结果发现:企业社交媒体交流可见性对员工工作场所学习具有显著正向影响;和谐式工作激情在企业社交媒体交流可见性与员工工作场所学习之间起中介作用;主动性人格正向调节企业社交媒体交流可见性与和谐式工作激情之间的关系,并且正向调节了和谐式工作激情在企业社交媒体交流可见性与员工工作场所学习之间的中介作用。本研究丰富和拓展了企业社交媒体交流可见性在工作场所发挥的作用,也为管理者深入理解企业社交媒体给组织带来的影响提供了理论指导。

关键词：企业社交媒体交流可见性和谐式工作激情主动性人格工作场所学习

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低温对民猪骨骼肌线粒体能量代谢基因的影响

西北农业学报 2024年第7期33卷 1195-1201页

作者：张冬杰马守正汪亮李忠秋刘娣黑龙江省农业科学院畜牧研究所哈尔滨150086

为了探究低温环境下民猪肌肉组织内与线粒体能量代谢相关基因的变化情况,以解析民猪抗寒特性的遗传机制。将9头体质量相近的母猪随机分成3组,每组3头,在冬季将其中2组置于室外半敞篷舍内饲养,分别处理3 d(急性低温处理组)和58 d(慢性低... 详细信息

为了探究低温环境下民猪肌肉组织内与线粒体能量代谢相关基因的变化情况,以解析民猪抗寒特性的遗传机制。将9头体质量相近的母猪随机分成3组,每组3头,在冬季将其中2组置于室外半敞篷舍内饲养,分别处理3 d(急性低温处理组)和58 d(慢性低温处理组),1组置于常规舍内饲养,作为对照组。以背肌为试验材料,采用PCR array方法,对90个与线粒体能量代谢相关基因的表达水平进行检测。结果表明,与对照组相比,急性低温处理组基因的表达水平差异不显著,但慢性低温处理显著改变 ATP5H、 ATP5L、 ATP6V1G3、 COX5A、 COX7A2、 COX7A2L、 NDUFS4、 SDHB、 UQCRQ和 GADD45B基因的表达水平,尤其是 GADD45B,达到了极显著水平(P<0.01),这些基因均为编码OXPHOS系统亚基基因,推测慢性低温诱导了背肌ATP的生成。对 GADD45B基因进一步分析发现,该基因在哺乳动物中高度保守,基本符合分子进化规律,表达具有明显的组织特异性。急性低温处理对民猪背肌内ATP的生成影响不显著,但慢性低温处理诱导ATP的生成,以维持体温恒定。

关键词：民猪骨骼肌低温线粒体能量代谢

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Vocal repertoire of Azure-winged Magpies(Cyanopica cyanus):A context-associated communication system

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Avian Research 2023年第1期14卷 45-53页

作者： Xu Wang Fan Yu Haozhong Si zhongqiu li Laboratory of Animal Behaviour and Conservation School of Life SciencesNanjing UniversityNarying 20023Chine

Signals within animals’vocal communication are considered functional referential and *** in the absence of the context,receivers are expected to acquire the information of calls and respond specificallyWhereas the framework was supported by plenty of evidence,its exhaustivity in describing all animal vocalisations has been ***,we investigated the vocal repertoire of a cooperatively breeding species,Azure-winged Magpie(Cyanopica cyanus),to present evidence for referential *** results showed that Azure-winged Magpies had a relatively large vocal repertoire,consisting of twelve distinct *** calls were associated with the context including movement,begging for food,contact,vigilance against predators,***,even the predator-specific alarm calls would induce various responses of *** implies that multiple pieces of information are involved in the vocalisation,which could be utilised by the receiver to select an appropriate response based on the *** study gives a detailed description of the context and function of the vocal repertoire in Azure-winged Magpies,laying the foundation for further investigation on the developmental mechanisms of bird *** study also suggests that the referential signals of animal vocalisations may not be limited to the context-specific responses of receivers and need to be discussed from a broader perspective.

关键词： Amure wingeod Magpies Behavioural ontext Reerential sigmals Vocal ammumication Vocal repertoire

Mechanical model for double side self-propelled rolling machine based on rigid and flexible contact dynamics

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International Journal of Agricultural and Biological Engineering 2022年第6期15卷 38-43页

作者： Yiming li Yu liu Xiang Yue zhongqiu li Xing'an liu Tianlai li College of Engineering Shenyang Agricultural UniversityShenyang 110866China College of Horticulture Shenyang Agricultural UniversityShenyang 110866China Key Laboratory of Protected Horticulture Shenyang Agricultural UniversityMinistry of EducationShenyang 110866China National&Local Joint Engineering Research Center of Northern Horticultural Facilities Design&Application Technology(liaoning) Shenyang 110866China

The objective of the present research was to establish a mechanical model to study the performance of double side self-propelled rolling *** are two key models in the modeling *** first model is the soft cover dynamics model,which is an important innovation in this *** the insulation quilt was established based on the Macro-modeling *** second model is the double side self-propelled rolling machine virtual prototype *** specifying multiple contact constraints and loadings between the soft cover dynamics model and the rigid component,the virtual prototype model was built successfully and the double side self-propelled rolling process was completely ***,the interaction mechanisms of the rigid and flexible coupling mechanics were *** virtual rolling processes of different insulation quilt lengths were analyzed under different thickness *** simulated results showed a good agreement with the experimental measurements,which suggested that the established model is an effective approach to evaluating and optimizing the rolling *** successful establishment of the mechanical model can facilitate the study of the performance of the product and further optimization,and also is of great significance to shorten the development cycle and reduce costs.

关键词： contact dynamics solar greenhouse self-propelled rolling machine mechanical model

Do bovids evolve hindquarter markings for anti-predation?

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Current Zoology 2022年第2期68卷 143-148页

作者： Cong Yu lixin CHEN Sihan NING Sana ULLAH zhongqiu li Laboratory of Animal Behavior and Conservation School of Life SciencesNanjing UniversityNanjing 210023China Department of Zoology Division of Science and TechnologyUniversity of EducationLahore 54000Pakistan

Conspicuous coloration in animals serves many functions such as ***-predation strategies include motion dazzle and flash *** dazzle markings can reduce the probability of being preyed on because the predators misjudge their *** flash behavior,prey demonstrate conspicuous cue while fleeing;the predators follow them;however,the prey hide their markings and the predators assume that the prey has *** investigate whether bovids use conspicuous hindquarter markings as an anti-predatory behavior,we undertook phylogenetically controlled analyses to explore under what physiological characteristics and environmental factors bovids might have this color *** results suggested that rump patches and tail markings were more prevalent in bovids living in larger-sized groups,which supports the hypothesis of intraspecific ***,we observed the occurrence of conspicuous white hindquarter markings in bovids having smaller body size and living in larger groups,suggesting a motion dazzle ***,the feature of facultative exposing color patterns(flash markings)was not associated with body size,which was inconsistent with predictions and implied that bovids may not adopt this as an anti-predator *** was concluded that species in bovids with conspicuous white hindquarter markings adopt motion dazzle as an anti-predation strategy while fleeing and escaping from being prey on.

关键词： anti-predation bovid flash behavior hindquarter markings interspecies communication motion dazzle

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广州管圆线虫感染大鼠脑组织转录组分析

中国寄生虫学与寄生虫病杂志 2024年第2期42卷 217-224,233页

作者：程东慧蒋天哥景一丹杨丽敏郭云海方圆李中秋张仪中国疾病预防控制中心寄生虫病预防控制所(国家热带病研究中心) 传染病溯源预警与智能决策全国重点实验室国家卫生健康委员会寄生虫病原与媒介生物学重点实验室世界卫生组织热带病合作中心科技部国家级热带病国际研究中心上海200025 上海交通大学医学院-国家热带病研究中心全球健康学院上海200025

目的了解广州管圆线虫感染后大鼠脑组织转录组的表达水平。方法将32只SD大鼠随机分为对照组(12只)和感染组(20只),感染组每只大鼠经灌胃感染40条广州管圆线虫Ⅲ期幼虫,对照组灌胃等体积生理盐水。感染后1、7、14、21 d,对照组、感染组... 详细信息

目的了解广州管圆线虫感染后大鼠脑组织转录组的表达水平。方法将32只SD大鼠随机分为对照组(12只)和感染组(20只),感染组每只大鼠经灌胃感染40条广州管圆线虫Ⅲ期幼虫,对照组灌胃等体积生理盐水。感染后1、7、14、21 d,对照组、感染组分别随机解剖3、5只,取脑组织制备石蜡切片,苏木精-伊红(HE)染色后观察脑组织病理变化。TRIzol法提取感染后14 d大鼠脑组织RNA,逆转录为cDNA后测序。选取差异表达mRNA进行基因本体论(GO)富集分析和京都基因与基因组百科全书(KEGG)代谢通路分析,采用STRING数据库预测差异表达mRNA靶蛋白之间的蛋白质互作(PPI)关系。利用生物信息学方法构建差异表达基因的竞争性内源性RNA(ceRNA)调控网络。采用qPCR验证表达差异的lncRNA。结果HE染色结果显示,感染广州管圆线虫后14 d,感染组大鼠脑组织出现病理变化,脑海马区神经元胞浆固缩;感染后21 d,脑膜处可见寄生虫样组织。RNA测序结果显示,感染广州管圆线虫后14 d,大鼠脑组织中差异表达mRNA的数量为955个(890个上调、65个下调);差异表达lncRNA的数量为193个(122个上调、71个下调)。GO富集分析结果显示,差异表达mRNA主要富集在炎症反应、免疫应答等生物过程,细胞成分主要为质膜外侧的胞外空间、细胞表面等,分子功能主要为趋化因子活性、趋化因子受体结合等;KEGG代谢通路分析结果显示,差异表达mRNA主要参与细胞因子-细胞因子受体相互作用、趋化因子等信号通路。PPI分析结果显示,差异表达基因主要的靶点为趋化因子配体11、RT1-Da、丝氨酸家族E成员1等,均与免疫应答相关。ceRNA结果显示,显著富集的miRNA如mir-466b-3p、mir-1956、mir-207和mir-328a-5p等与免疫应答、细胞凋亡、血管生成等过程有关。qPCR结果显示,感染广州管圆线虫后,感染组大鼠H19的相对转录水平逐渐升高,在感染后21 d达到峰值,为15.074±3.366,高于对照组的1.000±0.113(t=13.190,P<0.05);RT1-CE6、LOC100910973和lncR-ncf1的相对转录水平在感染后14 d达到峰值,分别为9.702±1.408、6.683±1.299和7.733±0.717,均高于对照组的1.003±0.039、1.001±0.156和0.999±0.076(t=20.760、13.830、28.810,均P<0.01);AABR07030796.1的相对转录水平在感染后14 d开始上升,至感染后21 d达到峰值,分别为4.485±0.236和5.068±1.608,均高于对照组的1.000±0.159和1.001±0.256(t=7.049、8.229,均P<0.01)。感染广州管圆线虫后14 d,大鼠脑组织中H19、RT1-CE6、LOC100910973、lncR-ncf1和AABR07030796.1的qPCR结果和RNA测序结果均显示上调,表达趋势一致。结论广州管圆线虫感染后大鼠脑组织转录组中获得955个差异表达mRNA和193个差异表达lncRNA,主要富集在炎症反应、免疫应答等生物过程。

关键词：广州管圆线虫长链非编码RNA 转录组学竞争性内源RNA

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Using a behavior random permutation model to identify displacement grooming in ungulates

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Current Zoology 2023年第2期69卷 200-207页

作者： Yunchao Luo lin Wang Le Yang Xiao-Fen li Patrick Anselme Xinxin Wang Xingjun Tian zhongqiu li Lab of Animal Behavior and Conservation School of Life SciencesNanjing UniversityNanjing 210023China Tibet Plateau Institute of Biology Lhasa 850000China Department of Electrical Engineering Shanghai Jiao Tong University800 DongChuan RoadShanghai 200240China Department of Biopsychology Ruhr-Universitat Bochum 44801BochumGermany

Behavior affects an individual's life in all aspects,e.g.,enhancing fitness,leveraging predation risk,and reducing competition with ***,the sequential distribution of behaviors received less attention and is unclear what the function of displacement behavior *** activities can be found in vertebrate species but there is no formal method to determine whether a behavior is expressed as a displaced or normal *** the sequential distributions of behaviors in a natural setting may allow researchers to identify unexpected distributions as a possible signature of displacement *** this study,we used a behavior random permutation model to detect the presence of a displacement activity in the Tibetan antelope Pantholops hodgsonii and the Tibetan gazelle Procapra *** results showed that grooming in both ungulates tended to be accompanied with vigilance,and the frequency of grooming after vigilance was significantly higher than before vigilance.A significant positive correlation between the scan rate and grooming rate in the 2 ungulates was *** suggest that grooming could sometimes be expressed as a displacement activity in *** addition to providing a general method for further research on displacement activities in a variety of animal species,this study sheds light on the importance of a spectral analysis of sequential distribution of animal *** random permutation models can be used to explore the relevance between any 2 behaviors in a specific sequence,especially to identify a myriad of unexpected behaviors relative to their normal context of occurrence.

关键词： behavior sequence displacement activity self-grooming vigilance random distribution behavioral analysis method