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Development of EST-SSR primers and their practicability test for Laminaria

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Acta Oceanologica Sinica 2011年第3期30卷 112-117页

作者： WANG Guoliang TAN Xiaolong SHEN Junling li Jian ZHANG Lei SUN Jianwei WANG Bin weng Manli liU Tao College of Marine life Science Ocean University of China Qingdao 266003 China institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing 100101 China College of Horticulture Qingdao Agricultural University Qingdao 266109 China

Laminaria （Saccharina） is one of the economic important seaweeds. Through sequence analysis of 4 099 ESTs from Laminaria （971 were generated from our own cDNA laboratories and 3 128 were downloaded from updated EST databases） with SSRIT software, two hundred and fifty-four SSRs in 206 ESTs were found. From the 254 SSRs, sixty-three SSR primer-pairs were designed. In order to test their practicability, the 63 primer-pairs were tested in commonly used SSR reaction conditions using 12 Laminara DNA samples as templates. The results show that 23 SSR primer-pairs gave good amplification patterns on most （more than 80%） of the 12 Laminara DNA templates. Genetic diversity study of 12 Laminaria lines, which were widely used in breeding and economic cultivation in China, was performed based on the obtained SSR data.

关键词： Laminaria EST-SSRs germplasm identification polymorphism analysis

福建省再生稻的生产现状及其增产潜力与发展对策

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亚热带资源与环境学报 2024年第3期19卷 86-91页

作者：翁伯琦刘岑薇陈华李艳春林怡姜照伟王义祥福建省农业科学院资源环境与土壤肥料研究所福州350013 福建省红壤山地农业生态过程重点实验室福州350013 福建省农业科学院水稻研究所福州350013

再生稻播一茬收两茬,具有生长期短、病虫害少、米质好等优点,是提高复种指数、增加粮食产量和种粮收入的重要措施。福建是中国最适宜种植再生稻的省份之一,绝大部分县市都可以种植再生稻。本研究分析了福建省再生稻发展现状及存在的主... 详细信息

再生稻播一茬收两茬,具有生长期短、病虫害少、米质好等优点,是提高复种指数、增加粮食产量和种粮收入的重要措施。福建是中国最适宜种植再生稻的省份之一,绝大部分县市都可以种植再生稻。本研究分析了福建省再生稻发展现状及存在的主要问题,阐述了福建省发展再生稻的优势所在与增产潜力,提出了促进再生稻发展与粮食产能的思路与对策建议,以求为优化福建省再生稻产业高质量发展体系提供参考与借鉴。

关键词：再生稻生产现状发展思路对策福建省

Effects of N Management on Yield and N Uptake of Rice in Central China

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Journal of Integrative Agriculture 2012年第12期11卷 1993-2000页

作者： PAN Sheng-gang HUANG Sheng-qi ZHAI Jing WANG Jing-ping CAO Cou-gui CAI Ming-li ZHAN Ming TANG Xiang-ru Key Laboratory of Huazhong Crop Physiology Ecology and Production Ministry of Agriculture/College of Plant Science & Technology Huazhong Agricultural University College of Agriculture South China Agricultural University

Efficient N fertilizer management is critical for the economic production of rice and the long-term protection of environmental quality. A field experiment was designed to study the effects of N fertilizer management practices on grain yield and N uptake office. The experiment was laid out in the randomized complete block design with four replications in Central China during 2008 and 2009. Five N treatments denoted as No, NIsoA, N15oB, N24OA, and N24oB, respectively, were studied. NO represented no N application and served as a control, N15OA and N15oB indicated the total N application of 150 kg N ha-I but with two different application schedules （A and B） across the early stage of rice growth. Schedule A was applied as follows： 40% basal, 30% at 10 d after transplanting （DAT） and 30% at 36 DAT （nearly at the panicle initiation stage）, while schedule B was as follows： 30% at basal, 20% at 10 DAT, and 50% at 36 DAT. Similarly, N240A and N240B indicated the total N application of 240 kg N ha-1 with schedules A and B as described above. To quantify N uptake from fertilizer and soil, a 15N experiment was also conducted within the main experimental field, with micro-plots. Grain yields were significantly increased as N rates increased from 0 to 240 kg N ha-1. At the same rate, splitting N application as schedule B significantly increased the grain yield, spikelets per panicle, percentage of ripened grain, and 1 000-grain weight, compared with the N application according to schedule A. Mean rice recovery of N fertilizer by ~SN tracing method ranged from 25.39% at N24oA to 34.89% at NIsoB, however, N fertilizer residual rate in the soil ranged from 12.40% at N240A to 16.61% at N^s0w About 31.5 and 28.5% of total uptake of ~SN derived from basal fertilizer was absorbed at panicle initiation and heading stages, respectively. However, 65.6-92.5% of total uptake of 15N derived from topdressing fertilizer was absorbed at the heading stage. Based on yield and nitrogen recovery e

关键词： 15SN N fate N management recovery rice

Analyzing the spatiotemporal evolution and driving forces of gross ecosystem product in the upper reaches of the Chaobai River Basin

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Frontiers of Environmental Science & Engineering 2024年第8期18卷 117-132页

作者： Jiacheng li Qi Han liqiu Zhang li Feng Guihuan liu Beijing Key Laboratory for Source Control Technology of Water Pollution College of Environmental Science and EngineeringBeijing Forestry UniversityBeijing 100083China Engineering Research Center for Water Pollution Source Control&Eco-remediation College of Environmental Science and EngineeringBeijing Forestry UniversityBeijing 100083China Center of Eco-compensation Chinese Academy of Environmental PlanningMinistry of Ecology of EnvironmentBeijing 100041China

The Chaobai River Basin,which is a crucial ecological barrier and primary water source area within the Beijing-Tianjin-Hebei region,possesses substantial ecological *** gross ecosystem product(GEP)in the Chaobai River Basin is a reflection of ecosystem conditions and quantifies nature’s contributions to humanity,which provides a basis for basin ecosystem service management and *** study investigated the spatiotemporal evolution of GEP in the upper Chaobai River Basin and explored the driving factors influencing GEP spatial *** patterns from 2005 to 2020 were analyzed,and GEP was calculated for 2005,2010,2015,and *** driving factors influencing GEP spatial differentiation were identified using the optimal parameter-based geographical detector(OPGD)*** key findings are as follows:(1)From 2005 to 2020,the main ecosystem types were forest,grassland,and *** areas experienced significant changes,and conversions mainly occurred among urban,water,grassland and agricultural ecosystems.(2)Temporally,the GEP in the basin increased from 2005 to 2020,with regulation services *** the county(district)scale,GEP exhibited a north-west-high and south-east-low pattern,showing spatial differences between per-unit-area GEP and county(district)GEP,while the spatial variations in per capita GEP and county(district)GEP were similar.(3)Differences in the spatial distribution of GEP were influenced by regional natural geographical and socioeconomic *** these factors,gross domestic product,population density,and land-use degree density contributed *** among different driving forces noticeably impacted GEP spatial *** findings underscore the necessity of incorporating factors such as population density and the intensity of land-use development into ecosystem management decision-making processes in the upper reaches of the Chaobai River *** policies

关键词： Ecosystem pattern Gross ecosystem product(GEP) Spatiotemporal evolution Optimal parameter-based geographical detector(OPGD) Chaobai River Basin

MXenes/CNTs-based hybrids:Fabrications,mechanisms,and modification strategies for energy and environmental applications

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Nano Research 2024年第5期17卷 3429-3454页

作者： Jizhou Jiang Fangyi li Lei Ding Chengxun Zhang Arramel Xin li School of Environmental ecology and Biological Engineering School of Chemistry and Environmental EngineeringKey Laboratory of Green Chemical Engineering Process of Ministry of EducationEngineering Research Center of Phosphorus Resources Development and Utilization of Ministry of EducationNovel Catalytic Materials of Hubei Engineering Research CenterWuhan Institute of TechnologyWuhan 430205China Nano Center Indonesia Jl.PUSPIPTEK Tangerang SelatanBanten 15314Indonesia institute of Biomass Engineering Key Laboratory of Energy Plants Resource and UtilizationMinistry of Agriculture and Rural AffairsSouth China Agricultural UniversityGuangzhou 510642China

Emerging two-dimensional(2D)layered metal carbide and nitride materials,commonly termed MXenes,are increasingly recognized for their applications across diverse fields such as energy,environment,and *** the past few years,MXenes/carbon nanotubes(CNTs)-based hybrids have attracted extensive attention as an important catalyst in energy and environmental fields,due to their superior multifunctions and mechanical *** review aims to address the fabrication strategies,the identification of the enhancement mechanisms,and recent progress regarding the design and modification of MXenes/CNTs-based hybrids.A myriad of fabrication techniques have been systematically summarized,including mechanical mixing,spray drying,three-dimensional(3D)printing,self-assembly/in-situ growth,freeze drying,templating,hydrothermal methods,chemical vapor deposition(CVD),and ***,the identification of the enhancement mechanisms was thoroughly discussed from the two dimensions of theoretical simulations and in-situ ***,the recent advancements in profound applications of MXenes/CNTs-based hybrids have also been carefully revealed,including energy storage devices,sensors,water purification systems,and microwave *** also underscore anticipated challenges related to their fabrication,structure,underlying mechanisms,modification approaches,and emergent ***,this review offers insights into prospective directions and the future trajectory for these promising *** is expected that this review can inspire new ideas or provide new research methods for future studies.

关键词： MXenes/carbon nanotubes(CNTs)-based hybrids fabrication applications modification strategies

mRNA vaccine technology for infectious diseases and beyond

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Science China(life Sciences) 2024年第10期67卷 2267-2270页

作者： Pei Hao Xiao li Xuan li Wu Zhong Key Laboratory of Molecular Virology and Immunology Shanghai Institute of Immunity and InfectionChinese Academy of SciencesShanghai 200031China Changchun Veterinary Research institute Chinese Academy of Agricultural SciencesChangchun 130122China Key Laboratory of Synthetic Biology CAS Center for Excellence in Molecular Plant SciencesInstitute of Plant Physiology and EcologyChinese Academy of SciencesShanghai 200032China National Engineering Research Center for the Emergency Drug Beijing Institute of Pharmacology and ToxicologyBeijing 100085China University of Chinese Academy of Sciences Beijing 100049China

The rise of mRNA vaccine technology Vaccines stand as one of the most significant medical advancements,playing a crucial role in the prevention of infectious diseases and the improvement of global *** triumph of smallpox vaccination stands as a towering achievement in the annals of healthcare,leading to the complete elimination of a oncedevastating disease and showcasing the efficacy of vaccination methods.

关键词： methods vaccination prevention

Inhibitory Effects of Aromatic Compounds on Soil Nitrification

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Pedosphere 2010年第3期20卷 326-333页

作者： ZHANG li-li WU Zhi-Jie SHI Yun-Feng CHEN li-Jun SONG Yu-Chao JUAN Ying-Hua institute of Applied ecology Chinese Academy of Sciences Shenyang 110016 （China） Graduate University of the Chinese Academy of Sciences Beijing 100049 （China） College of Resource and Environment Shenyang Agricultural University Shenyang 110039 （China）

Aromatic compounds (ACs) in soil can induce competitive inhibition for soil NH3 oxidation, and nitrification inhibitors can be used to this end. A laboratory incubation experiment was performed with 12 nitroaromatic compounds (NACs), 15 amidoaromatic compounds (AACs) and 20 hydroxyaromatic compounds (HACs) to assess the inhibitory effects of ACs on soil nitrification. Based on these results, the critical and optimal concentrations of ACs were determined for better inhibitory effects. Most of the test ACs were able to inhibit soil nitrification; the effectiveness differed with soil type. Among the ACs, the NACs with m-nitryl, amino or hydroxyl and the AACs with a nitro group or a chlorine atom on aromatic ring or with a p-hydroxyl were more effective. 3-nitroaniline, 4-aminophenol and 3-nitrophenol showed the greatest potential as nitrification inhibitors. The critical concentration of these compounds in brown soil and cinnamon soil was found to be 0.5 mg kg-1 soil. Due to the toxicity, carcinogenicity and mutagenicity of ACs, further toxicological and ecotoxicological research is necessary before ACs are used as nitrification inhibitors in agricultural and horticultural practices.

关键词： amidoaromatic compounds ammonia oxidation hydroxyaxomatic compounds nitrification inhibition nitroaromatic compounds

Enhancement of beneficial microbiomes in plant-soil continuums through organic fertilization:Insights into the composition and multifunctionality

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Soil ecology Letters 2024年第3期6卷 109-120页

作者： Wen-Jing li Xin-Yuan Zhou Xin-li An li-Juan li Chen-Shuo lin Hua li Hong-Zhe li Key Lab of Urban Environment and Health Institute of Urban EnvironmentChinese Academy of SciencesXiamen 361021China University of Chinese Academy of Sciences Beijing 100049China State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products Institute of EnvironmentResourceSoil and FertilizersZhejiang Academy of Agricultural SciencesHangzhou 310021China

Soil microbiomes play a crucial role in maintaining ecological functions and are of great importance for soil *** of them could bring benefits to plants for growth *** numerous studies have focused on specific beneficial bacteria and their interactions with soils and plants,we still lack a comprehensive understanding of beneficial communities in plant–soil continuums and their responses to agricultural *** address this gap,we carried out a microcosm experiment using 16S rRNA amplicon sequencing to explore the effects of organic fertilization on beneficial communities in plant–soil continuums and assess their potential *** findings reveal that organic fertilization had a positive impact on the beneficial functionality of bacterial communities in plant–soil *** improvement was primarily attributed to the optimized soil physicochemical conditions resulting from organic ***,organic fertilization increased the complexity of bacterial co-occurrence networks in both soils and the *** taxa in the endosphere undergone a shift of functions toward pathogen suppression as the result of organic ***,this study revealed that plants exhibited a preference for internalizing beneficial bacteria over other type of *** also provided new insights for evaluating the multifunctionality of microbiomes,and found that the functionality of beneficial communities in plant–soil continuums is enhanced by organic *** these findings suggested that organic fertilization can be an effective strategy for maintaining plant and soil health.

关键词： plant–soil continuums beneficial bacteria agricultural activities multifunctionality endosphere

Soil Organic Carbon Stocks of Citrus Orchards in Yongchun County Fujian Province China

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Pedosphere 2017年第5期27卷 985-990页

作者： WANG Yixiang weng boqi TIAN Na ZHONG Zhenmei WANG Mingkuang institute of agricultural ecology Fujian Academy of Agricultural Sciences

Studies related to the quantitation and distribution of soil organic carbon （C） under different land use types can help to fill the knowledge gaps regarding estimation of the amount of C stored in soils at a global scale. Orchards are an important land use type in southern China; the total area （1.15 x 107 ha） of orchards in China comprises approximately 20.5% of the area of all orchards worldwide. We assessed soil organic C stocks （SOCS） in citrus orchards in Yongchun County （consisting of 22 towns）, Fujian Province, China in 1982 and 2010. The southeastern part of the county, an area featuring extensive citrus farming with a gently sloping landscape and low rates of water erosion, had the highest SOCS. In contrast, the lowest SCOS was observed in the northern part of the county, where steep hills with severe erosion problems are common and citrus orchards are sparsely distributed. From 1982 to 2010, the mean SOCS in citrus orchards increased from 22.1 to 41.7 Mg ha-1 which indicated that current management practices such as fertilization, irrigation, and cultivation enhanced soil C stocks. Further in-depth studies on the effects of these practices on C sequestration would be worthwhile and surveys of SOCS throughout Fujian Province are needed, to support efforts to mitigate global C emission.

关键词： carbon emission carbon pool carbon sequestration kriging land use type management practices