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QCM and EC-SPR Studies of Cytochrome c Self-assembled on Au Electrode and Enhancement of SPR Signal by Au Nanoparticles

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Chemical Research in Chinese Universities 2012年第6期28卷 1061-1065页

作者： WANG yan-yan JIANG yan-xla ZHOU You-chen li yan-yan ma Zhi-fang SUN Shi-gang State Key Laboratory of Physical Chemistry of Solid Surfaces Department of ChemistryCollege of Chemistry and Chemical Engineering Xiamen University Xiamen 361005 P. R. China

Quartz crystal microbalance（QCM） and cyclic voltammetry（CV） were used to characterize the monolayer of cytochrome c（Cyt c）,which was adsorbed on gold film modified with alkanethiol mixed monolayer.A direct comparison of protein surface coverages calculated from QCM and cyclic voltammetric measurements illustrates that the ratio of the electroactive Cyt c to the total surface-confined Cyt c is 34%,which suggests that the orientation is a main factor affecting the electroactivity of Cyt ***,surface plasmon resonance（SPR） measurement combined with CV ＂in situ＂ was used to investigate the conformational change of Cyt c in the redox ***,Au nanoparticles（Au NPs） were adsorbed on the surface of Cyt *** result indicates that Au NPs promote electron transfer between Cyt c and the gold electrode,and SPR result suggests Au NPs enhance SPR signal.

关键词： Cytochrome c Gold film Self-assembly Electrochemical-surface plasmon resonance(EC-SPR)

Relationship between VEGF,EGF and Invasion,Metastasis of Gastric Cancer Cells

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Chinese Journal of Cancer Research 2009年第2期21卷 122-129页

作者： Chun-feng li yan ma Yu-zhe Wei Ying-wei Xue Department of Abdominal Surgery Cancer Hospital Harbin Medical University Harbin 150081 China

Objective： To investigate the relationship between expression of vascular endothelial growth factor （VEGF）, epidermal growth factor （EGF） and the biological properties of gastric cancer cells such as invasion and metastasis. Methods： RT-PCR was performed to semi-quantitatively detect the mRNA expressions of EGF, EGFR, VEGF and VEGFR in four kinds of gastric cancer cell lines BGC823, MGCS03, HGC27 and SGC7901, which were classified by their differentiation degree in our experiment. We obtained cell line growth curves from MTT assays. The migration of gastric cancer cells was observed under inverted phase contrast microscope. The changes of invasion and adhesion were detected by a Transwell assay. Results： The growth rates slowed down sequentially in MGC803, HGC27, BGC823 and SGC7901（P〈 0.05）. The ability of migration, invasion and adhesion were reduced sequentially, and the difference was significant. The expressions of EGF, EGFR, VEGF and VEGFR were significantly stronger in MGCS03 and HGC27 than in BGC823 and SGC7901 cells, and the difference was statistically significant（P〈0.05）. Conclusion： The expressions of VEGF and EGF had close relationship with the properties of migration, adhesion and invasion of gastric cancer cells in vitro. Thus, targeting VEGF and EGF may be a potential therapeutic strategy for inhibiting peritoneal metastasis of gastric cancer.

关键词： Gastric cancer cells Vascular endothelial growth factor(VEGF) Epidermal growth factor(EGF),Metastasis

Common features in patients with intracerebral hemorrhage following superficial temporal artery-middle cerebral artery bypass in steno-occlusive cerebrovascular disease

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Neural Regeneration Research 2012年第20期7卷 1585-1590页

作者： Zhiqi mao Meng li yan ma yanfei Chen Hongqi Zhang Feng ling Department of Neurosurgery Xuanwu Hospital Capital Medical University Beijing 100053 China

Five patients treated for intracranial cerebral hemorrhage after superficial temporal artery-middle cerebral artery bypass in Xuwu Hospital, Capital Medical University, Beijing, China, from 2005-2011 were included in this study. Prior to superficial temporal artery-middle cerebral artery bypass, all patients showed diminished cerebrovascular reactivity and an ipsilateral ischemic lesion. Intracranial cerebral hemorrhage developed within 1-4 days following superficial temporal artery-middle cerebral artery bypass. Transcranial Doppler showed increased middle cerebral artery velocity of 50-100% in the operated hemisphere. These findings suggested that focal hyperperfusion, an ipsilateral ischemic lesion and diminished cerebrovascular reactivity are the important characteristics of intracerebral hemorrhage following superficial temporal artery-middle cerebral artery bypass in patients with steno-occlusive cerebrovascular disease.

关键词： cerebral hemorrhage STA-MCAo ,cclusion cerebrovascuiar disordersS uperfiCihy alperperfusionte mp ral artery-middlis echemic lesion cerebrovascular reactivityC erebral artery bypass stenosis

A new cassane diterpenoid lactone from the seed of Caesalpinia minax

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Chinese Chemical Letters 2009年第4期20卷 444-446页

作者： yan Cheng li yan ma Jian Hua Miao Xu Dong Xu Jing Quan Yuan Jun Shan yang Institute of Medicinal Plant Development Peking Union Medical College and Chinese Academy of Medical Sciences Beijing 100193 People's Republic of China Guangxi Botanical Garden of Medical Plant Nanning 530023 People's Republic of China

A new cassane diterpenoid lactone was isolated from the seed of Caesalpinia minax. On the basis of spectral evidences, its structure was established as 12α-methoxyl, 5α, 14β-dihydroxy-1α, 6α, 7β-triacetoxycass-1... 详细信息

关键词： Caesalpinia Caesalpinia minax Cassane diterpenoid lactone

Cytosolic calcium oscillations in submandibular gland cells

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Acta Pharmacologica Sinica 2006年第7期27卷 843-847页

作者： li SU Chun-yan ma yan-dong ZHOU yan-hong JIA Zong-jie CUI~2 Institute of Cell Biology,Beijing Normal University,Beijing 100875,China Institute of Cell Biology Beijing Normal University Beijing China

Calcium oscillations can,by default,encode diverse and specific signals by differ-ent modes of *** modulation is illustrated by the activation ofcalcium/calmodulin-dependent protein kinase Ⅱ at unit Hz,and of calcineurin at 10mHz frequencies,*** submandibular gland secretory axis is charac-terized by both potassium and osmolarity gradients from the luminal side of thesecretory *** gradients may play significant physiological roles throughthe feedback modulation of cholinergic *** potassium transformsplateau calcium increases induced by cholinergic stimulation of the submandibu-lar acinar cells into oscillatory calcium *** ductal cells may have simi-lar mechanisms of feedback modulation both by high potassium and by *** feedback mechanisms could modulate the decision-making pro-cess for determining which secretory products are selectively released after nervestimulation.

关键词： submandibular gland secretory axis calcium oscillation

Coronary collateral circulation： Effects on outcomes of acute anterior myocardial infarction after primary percutaneous coronary intervention

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Journal of Geriatric Cardiology 2011年第2期8卷 93-98页

作者： Bin Wang Ya-ling Han Yi li Quan-Min Jing Shou-li Wang Ying-yan ma Geng Wang Bo Luan Xiao-Zeng Wang Department of Cardiology Shenyang North Hospital Shenyang 110016 Liao Ning Province China

Background To investigate the effects of collateral coronary circulation on the outcome of the patients with anterior myocardial infarction （MI） with left anterior desending artery occlusion abruptly. Methods Data of 189 patients with acute anterior MI who had a primary percutaneous coronary intervention （PCI） in the first 12 h from the onset of symptoms between January 2004 and December 2008 were retrospective analyzed. Left anterior descending arteries （LAD） of all patients were occluded. LADs were reopened with primary PCI. According to the collateral circulation, all patients were classified to two groups： no collateral group （n = 111）, patients without angiographic collateral filling of LAD or side branches （collateral index 0） and collateral group （n = 78）, and patients with angiographic collateral filling of LAD or side branches （collateral index 1, 2 or 3）. At one year＇ s follow-up, the occurrence of death, reinfarctlon, stent thrombosis （ST）, target vessel revascularization and readmission because of heart failure were observed. Results At one year, the mortality was lower in patients with collateral circulation compared with those without collateral circulation （1% vs. 8%, P = 0.049）, whereas there Were no differences in the occurrence of reinfarction, ST, target vessel revascularization and readmission because of heart failure. The occurrence of composite of endpoint was lower in patients with collateral circulation compared with those without collateral circulation （12% vs. 26%; P = 0.014）. Conclusions Pre-exist collateral circulation may prefigure the satisfactory prognosis to the patients with acute anterior MI after primary PCI in the first 12 h of MI onset.

关键词： collateral circulation myocardial infarction mortality left anterior descending artery

Diagonal loading least squares time delay estimation

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Chinese Journal of Acoustics 2012年第2期31卷 165-177页

作者： li Xuan yan Shefeng ma Xiaochuan Institute of Acoustics The Chinese Academy of Sciences Beijing 1000190

Least squares （LS） time delay estimation is a classical and effective method. However, the performance is degraded severely in the scenario of low ratio of signal-noise （SNR） due to the instability of matrix inversing. In order to solve the problem, diagonal loading least squares （DL-LS） is proposed by adding a positive definite matrix to the inverse matrix. Furthermore, the shortcoming of fixed diagonal loading is analyzed from the point of regularization that when the tolerance of low SNR is increased, veracity is decreased. This problem is resolved by reloading. The primary estimation＇s reciprocal is introduced as diagonal loading and it leads to small diagonal loading at the time of arrival and larger loading at other time. Simulation and pool experiment prove the algorithm has better performance.

关键词：对角加载最小二乘时延估计时间延迟估计低信噪比矩阵反演正定矩阵水池实验

Fabrication of the DNA/poly(3-methylthiophene) composite film modified electrode and its application for the study on the voltammetric behavior and determination of 8-hydroxy-2'-deoxyguanosine

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Science China Chemistry 2009年第11期52卷 2006-2012页

作者： WANG yanHuai,li Jing,liU yan,ma RongNa,JIA Wenli,CUI Hui & WANG HuaiSheng College of Chemistry and Chemical Engineering,Institute of Analytical Chemistry,liaocheng University,liaocheng 252059,China College of Chemistry and Chemical Engineering Institute of Analytical Chemistry Liaocheng University Liaocheng China

A composite film of DNA/poly(3-methylthiophene)(P3MT) modified glassy carbon electrode(GCE) has been fabricated by electro-deposition method.P3MT film was first electropolymerized at the GCE and the DNA layer was then immobilized on the P3MT layer by electrochemical *** voltammetric behavior of 8-hydroxy-2'-deoxyguanosine(8-OH-dG) at the composite film modified electrode was *** effects of scan rates,pH and the interference of uric acid(UA) on the voltammetric behavior and detection of 8-OH-dG were also *** experimental results suggest that the electrochemical behavior of 8-OH-dG at the composite film modified electrode was greatly improved due to the combination of the advantages of P3MT and *** 0.1 M pH 7.0 phosphate buffer solution(PBS),the anodic peak currents of 8-OH-dG were linear with the 8-OH-dG concentration in two intervals,viz.0.28―4.2 μM and 4.2―19.6 μ*** detection limit of 56 nM 8-OH-dG could be estimated(S/N=3).This proposed composite film modified electrode shows excellent reproducibility and *** may have the potential application for the detection of 8-OH-dG in human urine.

关键词： poly(3-methylthiophene) DNA 8-hydroxy-2’-deoxyguanosine composite film modified electrode

Theoretical characterization of the temperature-dependent saturation magnetization of magnetic metallic materials

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Chinese Physics B 2024年第7期33卷 577-585页

作者：吴金龙董攀贺屹马艳丽李梓源姚沁远邱俊麻建坐李卫国 State Key Laboratory of Coal Mine Disaster Dynamics and Control Chongqing UniversityChongqing 400044China College of Aerospace Engineering Chongqing UniversityChongqing 400044China College of Intelligent Systems Science and Engineering Hubei Minzu UniversityEnshi 445000China College of Mechanical Engineering and Automation Chongqing Industry Polytechnic CollegChongqing 401120China

Based on the force-heat equivalence energy density principle,a theoretical model for magnetic metallic materials is developed,which characterizes the temperature-dependent magnetic anisotropy energy by considering the equivalent relationship between magnetic anisotropy energy and heat energy;then the relationship between the magnetic anisotropy constant and saturation magnetization is ***,we formulate a temperature-dependent model for saturation magnetization,revealing the inherent relationship between temperature and saturation *** model predicts the saturation magnetization for nine different magnetic metallic materials at different temperatures,exhibiting satisfactory agreement with experimental ***,the experimental data used as reference points are at or near room *** to other phenomenological theoretical models,this model is considerably more accessible than the data required at 0 *** index included in our model is set to a constant value,which is equal to 10/3 for materials other than Fe,Co,and *** transition metals(Fe,Co,and Ni in this paper),the index is 6 in the range of 0 K to 0.65T_(cr)(T_(cr) is the critical temperature),and 3 in the range of 0.65T_(cr) to T_(cr),unlike other models where the adjustable parameters vary according to each *** addition,our model provides a new way to design and evaluate magnetic metallic materials with superior magnetic properties over a wide range of temperatures.

关键词： magnetic metallic materials temperature dependent saturation magnetization modeling