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Journal of Harbin Institute of Technology(New Series) 2024年第1期31卷 16-25页

作者： Xinyue liu yaling Huang Yuyao li Jie liu Quanfang Chen Department of Electrical Engineering Southwest Jiaotong UniversityChengdu 610031China

Graphene(Gr)has unique properties including high electrical conductivity;Thus,graphene/copper(Gr/Cu)composites have attracted increasing attention to replace traditional Cu for electrical applications. However,the problem of how to control graphene to form desired Gr/Cu composite is not well solved. This paper aims at exploring the best parameters for preparing graphene with different layers on Cu foil by chemical vapor deposition(CVD)method and studying the effects of different layers graphene on Gr/Cu composite’s electrical conductivity. Graphene grown on single-sided and double-sided copper was prepared for Gr/Cu and Gr/Cu/Gr composites. The resultant electrical conductivity of Gr/Cu composites increased with decreasing graphene layers and increasing graphene volume fraction. The Gr/Cu/Gr composite with monolayer graphene owns volume fraction of less than 0.002%,producing the best electrical conductivity up to59.8 ×10^(6)S/m,equivalent to 104.5% IACS and 105.3% pure Cu foil.

关键词： chemical vapor deposition(CVD) Gr/Cu Gr/Cu/Gr graphene layers graphene volume fraction electrical conductivity

Gene-knockout by iSTOP enables rapid reproductive disease modeling and phenotyping in germ cells of the founder generation

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Science China(life Sciences) 2024年第5期67卷 1035-1050页

作者： yaling Wang Jingwen Chen Xueying Huang Bangguo Wu Peng Dai Feng Zhang Jinsong li lingbo Wang State Key Laboratory of Genetic Engineering Shanghai Key Laboratory of Metabolic Remodeling and HealthInstitute of Metabolism and Integrative BiologyFudan UniversityShanghai 200438China Institute of Reproduction and Development Obstetrics and Gynecology HospitalFudan UniversityShanghai 200011China NHC Key Lab of Reproduction Regulation(Shanghai Institute for Biomedical and Pharmaceutical Technologies) School of PharmacyFudan UniversityShanghai 200433China Shanghai Key Laboratoryof Maternal and Fetal Medicine Clinical and Translational Research Center of Shanghai First Maternity and Infant HospitalFrontier Science Center for Stem Cell ResearchSchoolof Life Sciences and TechnologyTongji UniversityShanghai 200092China State Key Laboratory of Cell Biology CAS Center for Excellence in Molecular Cell ScienceShanghai Institute of Biochemistry and Cell BiologyChinese Academy of SciencesShanghai 200031China

Cytosine base editing achieves C·G-to-T·A substitutions and can convert four codons(CAA/CAG/CGA/TGG)into STOP-codons(induction of STOP-codons,iSTOP)to knock out genes with reduced *** enables direct phenotyping in founders’somatic cells,but it remains unknown whether this works in founders’germ cells so as to rapidly reveal novel genes for ***,we initially establish that iSTOP in mouse zygotes enables functional characterization of known genes in founders’germ cells:Cfap43-iSTOP male founders manifest expected sperm features resembling human“multiple morphological abnormalities of the flagella”syndrome(i.e.,MMAF-like features),while oocytes of Zp3-iSTOP female founders have no zona *** further illustrate iSTOP’s utility for dissecting the functions of unknown genes with Ccdc183,observing MMAF-like features and male infertility in Ccdc183-iSTOP founders,phenotypes concordant with those of Ccdc183-KO *** ultimately establish that CCDC183 is essential for sperm morphogenesis through regulating the assembly of outer dynein arms and participating in the intra-flagellar *** study demonstrates iSTOP as an efficient tool for direct reproductive disease modeling and phenotyping in germ cells of the founder generation,and rapidly reveals the essentiality of Ccdc183 in fertility,thus providing a time-saving approach for validating genetic defects(like nonsense mutations)for human infertility.

关键词： disease modeling infertility multiple morphological abnormalities of the flagella(MMAF) induction of STOP-codons(iSTOP CRISPR-STOP) sperm motility

Graphene Nanoribbons Enhancing the Electronic Conductivity and Ionic Diffusion of Graphene Electrodes for High-Performance Microsupercapacitors

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Energy & Environmental Materials 2024年第4期7卷 285-294页

作者： Yan Zhang Huandi Zhang Xiaoxiao Wang Xiaowei Shi Zehua Zhao yaling Wang Jiamei liu Cheng Tang Guolong Wang Lei li State Key Laboratory for Mechanical Behavior of Materials School of Materials Science and Engineering Xi'an Jiaotong University Center for Materials Chemistry Frontier Institute of Science and TechnologyXi'an Jiaotong University Instrument Analysis Center of Xi'an Jiaotong University Xi'an Jiaotong University

The electrochemical performance of microsupercapacitors with graphene electrodes is reduced by the issue of graphene sheets aggregation,which limits electrolyte ions penetration into *** the space between graphene sheets in electrodes facilitates the electrolyte ions penetration,but sacrifices its electronic conductivity which also influences the charge storage ability.

关键词： areal energy density graphene graphene nanoribbons microsupercapacitors structure engineering

Water-soluble organic matter with various polarities in PM_(2.5) over Xi'an,China:Abundance,functional groups,and light absorption

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Particuology 2024年第1期84卷 281-289页

作者： Xueting Yang Shasha Huang Dan li Hongmei Xu yaling Zeng liu Yang Diwei Wang Ningning Zhang Junji Cao Zhenxing Shen Department of Environmental Science and Engineering Xi'an Jiaotong UniversityXi'an710049China School of Environmental Science and Engineering Southern University of Science and TechnologyShenzhen518055China State Key Laboratory of Loess and Quaternary Geology Institute of Earth EnvironmentChinese Academy of SciencesXi'an710049China

Humic-like substances(HUliS)are a major component of brown carbon and consequently play a major role in climate *** this study,70 PM_(2.5) samples were collected from Xi'an in winter 2019 and summer *** HUliS(HUliS-n),acidic HUliS(HUliS-a),and high-polarity water-soluble organic compounds(HP-WSOC)were analyzed to determine their carbon concentrations and measure their ultraviolet-visible absorption and infrared *** the three components,HUliS-n had the highest carbon content in both winter(3.29±1.45μg m^(-3))and in summer(1.38±1.10μg m^(-3)).The semi-quantitative results for the functional groups revealed that HP-WSOC was rich in carboxylic acids and had high aromaticity in winter,whereas HUliS-n was rich in carboxylic acids in ***,HULJS-a was richer in nitrate esters and saturated aliphatic hydrocarbons in summer than in *** results for specific ultraviolet absorbance(SUVA)and E_(250)/E_(365) revealed that HUliS had higher molecular weight and aromaticity in winter than in ***-n dominated in the total light absorption of HUliS+HP-WSOC in both winter(73.08%)and summer(48.57%).Overall,the results on the carbon content,optical properties,and functional groups of WSOCs with differing polarity can improve un-derstanding of environmental and climatic effects.

关键词： Humic-like substances(HUliS) Aromaticity Fourier-transform infrared(FTIR) spectroscopy light absorbance

支气管肺发育不良早产儿父母出院准备需求的质性研究

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护士进修杂志 2024年第2期39卷 204-208页

作者：余丽红李亚玲彭娟郭庆娇肖诗兰冯安丽游晶贵州健康职业学院贵州铜仁554300 贵州医科大学附属医院贵州贵阳550001 贵州医科大学护理学院贵州贵阳550025

目的从新生儿重症监护室(NICU)支气管肺发育不良(BPD)早产儿父母的角度探讨其出院准备情况及居家照护信息需求,为采取科学有效的出院准备干预方案提供依据。方法采用目的抽样方法,选取2021年12月-2022年8月入住贵州医科大学附属医院N... 详细信息

目的从新生儿重症监护室(NICU)支气管肺发育不良(BPD)早产儿父母的角度探讨其出院准备情况及居家照护信息需求,为采取科学有效的出院准备干预方案提供依据。方法采用目的抽样方法,选取2021年12月-2022年8月入住贵州医科大学附属医院NICU的12名BPD早产儿父母为研究对象,采用质性研究的方法对BPD早产儿父母进行半结构式深入访谈,采用Colaizzi现象学分析法整理分析资料。结果提炼出4个主题,即多样化知识需求、角色适应不足、出院准备不确定和情绪变化。结论 BPD患儿父母表示需要充分的准备和个性化的指导,以减少在过渡准备出院期间的焦虑和不确定性。因此需提高照顾技能和疾病相关知识,有助于父母的角色适应,并且多学科团队的支持指导可能对出院准备过程至关重要,尤其针对BPD早产儿的后期随访服务,为支气管肺发育早产儿家庭出院后延续性护理服务提供参考依据。

关键词：支气管肺发育不良照顾者早产儿出院准备度质性研究照护

Global characterization of OsPIP aquaporins reveals that the H_(2)O_(2)transporter OsPIP2;6 increases resistance to rice blast

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The Crop Journal 2024年第1期12卷 102-109页

作者： Gousi li Jingluan Han Chen Yi Hao Luo Yuzhu Wang Fengpin Wang Xiaoyu Wang Letian Chen yaling Zhang State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources College of Life SciencesSouth China Agricultural UniversityGuangzhou 510642GuangdongChina Guangdong Laboratory for lingnan Modern Agriculture Guangzhou 510642GuangdongChina National Key Laboratory of Plant Molecular Genetics Shanghai Center for Plant Stress BiologyCAS Center for Excellence in Molecular Plant SciencesShanghai Institute of Plant Physiology and EcologyChinese Academy of SciencesShanghai 200032China

Plasma membrane intrinsic proteins(PIPs)are conserved plant aquaporins that transport small molecules across the plasma membrane to trigger instant stress responses and maintain cellular homeostasis under biotic and abiotic *** elucidate their roles in plant immunity to pathogen attack,we characterized the expression patterns,subcellular localizations,and H_(2)O_(2)-transport ability of 11 OsPIPs in rice(Oryza sativa),and identified OsPIP2;6 as necessary for rice disease ***2;6 resides on the plasma membrane and facilitates cytoplasmic import of the immune signaling molecule H_(2)O_(2).Knockout of OsPIP2;6 increases rice susceptibility to Magnaporthe oryzae,indicating a positive function in plant ***2;6 interacts with OsPIP2;2,which has been reported to increase rice resistance to pathogens via H_(2)O_(2)*** findings suggest that OsPIP2;6 cooperates with OsPIP2;2 as a defense signal transporter complex during plant–pathogen interaction.

关键词： Aquaporin Plant immunity Rice blast H_(2)O_(2)transport

In situ high-quality liF/li_(3)N inorganic and phenyl-based organic solid electrolyte interphases for advanced lithium–oxygen batteries

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Carbon Energy 2024年第9期6卷 29-38页

作者： Qianyan Wang Minsheng Wu Yunkai Xu Chuyue li Yuanjia Rong yaling liao Menglin Gao Xiaoping Zhang Weirong Chen Jun Lu School of Electrical Engineering Southwest Jiaotong UniversityChengduChina College of Chemical and Biological Engineering Zhejiang UniversityHangzhouChina

lithium metal shows a great advantage as the most promising anode for its unparalleled theoretical specific capacity and extremely low electrochemical ***,uncontrolled lithium dendrite growth and severe side reactions of the reactive intermediates and organic electrolytes still limit the broad application of lithium metal ***,we propose 4-nitrobenzenesulfonyl fluoride(NBSF)as an electrolyte additive for forming a stable organic-inorganic hybrid solid electrolyte interphase(SEI)layer on the lithium *** abundance of lithium fluoride and lithium nitride can guarantee the SEI layer's toughness and high ionic conductivity,achieving dendrite-free lithium ***,the phenyl group of NBSF significantly contributes to both the chemical stability of the SEI layer and the good adaptation to volume changes of the lithium *** lithium-oxygen batteries with NBSF exhibit prolonged cycle lives and excellent cycling *** simple approach is hoped to improve the development of the organic-inorganic SEI layer to stabilize the lithium anodes for lithium-oxygen batteries.

关键词： lithium anode lithium-oxygen batteries reactive oxygen species solid electrolyte interphase

龙岩市部分规模化猪场猪粪便寄生虫检测与分析

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福建畜牧兽医 2024年第4期46卷 30-33页

作者：朱金玲林俊宏黄思琼李雅铃王少榕黄翠琴黄剑梅龙岩学院生命科学学院福建龙岩364000 福建农林大学动物科学学院福州350002 龙岩欣牧生物科技有限公司福建龙岩364000

目的:了解龙岩市5个区(市、县)的部分规模化猪场猪寄生虫感染情况,更好地保护猪群健康。方法:收集临床猪粪便样品,采用直接涂片法进行猪粪便寄生虫检测。结果:采集的229份粪便样品中,有101份检测出寄生虫,寄生虫感染率为44.11%(101/229)... 详细信息

目的:了解龙岩市5个区(市、县)的部分规模化猪场猪寄生虫感染情况,更好地保护猪群健康。方法:收集临床猪粪便样品,采用直接涂片法进行猪粪便寄生虫检测。结果:采集的229份粪便样品中,有101份检测出寄生虫,寄生虫感染率为44.11%(101/229),检测到3种猪寄生虫,分别为猪结肠小袋纤毛虫、猪球虫和猪蛔虫,其检出率分别为32.75%(75/229)、14.41%(33/229)和0.44%(1/229)。按采样季节分析寄生虫感染情况,发现春季的检出率为35.38%(23/65),夏季的检出率为51.06%(48/94),秋季的检出率为29.41%(5/17),冬季的检出率为47.17%(25/53)。结论:龙岩市部分规模化猪场存在寄生虫感染,且以土源性寄生虫感染为主,未发现生物源性寄生虫感染,本研究可为龙岩市猪寄生虫病防控提供参考依据。

关键词：规模化猪场直接涂片法结肠小袋纤毛虫猪球虫猪蛔虫

精神科护士使用保护性约束的知信行量表编制及信效度检验

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四川精神卫生 2024年第2期37卷 137-143页

作者：叶君荣王浩云王雯肖爱祥吴晨欣王莉夏志春姜恋李亚玲于林黄杏笑杨航广州医科大学附属脑科医院广东广州510370 广州医科大学护理学院广东广州511436 江门市第三人民医院广东江门529000 广州市民政局精神病医院广东广州510430

背景当前,我国关于精神科护士使用保护性约束知信行的测量工具未充分考虑精神科专科特点,且测评条目繁冗,不利于推广应用,亟需更为科学的测量工具对精神科护士使用保护性约束的知信行水平进行评定。目的构建精神科护士使用保护性约束的... 详细信息

背景当前,我国关于精神科护士使用保护性约束知信行的测量工具未充分考虑精神科专科特点,且测评条目繁冗,不利于推广应用,亟需更为科学的测量工具对精神科护士使用保护性约束的知信行水平进行评定。目的构建精神科护士使用保护性约束的知信行量表,并验证其信效度,为评估精神科护士使用保护性约束的知信行水平提供工具。方法基于知信行理论,通过查阅文献、访谈以及德尔菲法形成初版量表。采用分层抽样法,于2021年7月—12月,选取广东省内三级、二级和一级(含未评定等级和基层医院)各2家、共729名精神科护士为研究对象。通过项目分析、探索性因子分析、验证性因子分析形成正式量表,并采用内容效度、结构效度评价量表的效度,采用Cronbach’sα系数、重测信度、折半信度评价量表的信度。结果精神科护士使用保护性约束的知信行量表共12个条目,分为知识、信念、行为三个维度。量表水平的内容效度指数(S-CVI)为0.941,各条目的内容效度指数(I-CVI)为0.812~1.000。探索性因子分析共提取3个公因子,累计方差贡献率为62.948%。验证性因子分析结果显示模型拟合良好。量表的Cronbach’sα系数为0.887,其中知识、信念和行为维度的Cronbach’sα系数分别为0.819、0.842和0.831,量表的折半信度为0.712,重测信度为0.922。结论精神科护士使用保护性约束的知信行量表具有良好的信效度,可用于临床工作中对护理人员保护性约束知识、态度及行为的测评。

关键词：精神科护士保护性约束知信行量表编制信度效度

Coiled-coil domain-containing 38 is required for acrosome biogenesis and fibrous sheath assembly in mice

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Journal of Genetics and Genomics 2024年第4期51卷 407-418页

作者： yaling Wang Xueying Huang Guoying Sun Jingwen Chen Bangguo Wu Jiahui Luo Shuyan Tang Peng Dai Feng Zhang Jinsong li lingbo Wang Shanghai Key Laboratory of Metabolic Remodeling and Health Institute of Metabolism and Integrative BiologyFudan UniversityShanghai 200438China Institute of Reproduction and Development Obstetrics and Gynecology HospitalFudan UniversityShanghai 200011China Shanghai Key Laboratory of Maternal and Fetal Medicine Clinical and Translational Research Center of Shanghai First Maternity and Infant HospitalFrontier Science Center for Stem Cell ResearchSchool of Life Sciences and TechnologyTongji UniversityShanghai 200092China State Key Laboratory of Cell Biology CAS Center for Excellence in Molecular Cell ScienceShanghai Institute of Biochemistry and Cell BiologyChinese Academy of SciencesShanghai 200031China

During spermiogenesis,haploid spermatids undergo dramatic morphological changes to form slender sperm flagella and cap-like acrosomes,which are required for successful *** deformities in flagella cause a male infertility syndrome,multiple morphological abnormalities of the flagella(MMAF),while acrosomal hypoplasia in some cases leads to sub-optimal embryonic developmental ***,evidence regarding the occurrence of acrosomal hypoplasia in MMAF is ***,we report the generation of base-edited mice knocked out for coiled-coil domain-containing 38(Ccdc38)via inducing a nonsense mutation and find that the males are *** Ccdc38-KO sperm display acrosomal hypoplasia and typical MMAF *** find that the acrosomal membrane is loosely anchored to the nucleus and fibrous sheaths are disorganized in Ccdc38-KO *** analyses reveal that Ccdc38 knockout causes a decreased level of TEKT3,a protein associated with acrosome biogenesis,in testes and an aberrant distribution of TEKT3 in *** finally show that intracytoplasmic sperm injection overcomes Ccdc38-related *** study thus reveals a previously unknown role for CCDC38 in acrosome biogenesis and provides additional evidence for the occurrence of acrosomal hypoplasia in MMAF.

关键词： Acrosomal hypoplasia Multiple morphological abnormalities of the flagella(MMAF) Infertility Sperm motility Asthenoteratozoospermia Disease modeling