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Single nucleotide polymorphism analysis of the encoding region of EX-FABP gene and its association with fattiness trait in chicken

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Progress in Natural science:Materials International 2002年第3期 78-80页

作者： WANG Qigui, li Ning, HU Xiaoxiang, deng xuemei, liAN Zhengxing,li Hui and WU Changxin( National Laboratory for Agribiotechnology, china agricultural university, beijing 100094, china college of animal science and technology, Northeast agricultural university, Harbin 150030, china college of animal science and technology, china agricultural university, beijing 100094, china) National Laboratory for Agribiotechnology，china agricultural university，beijing 100094，china college of animal science and technology，Northeast agricultural university，Harbin 150030，china National Laboratory for Agribiotechnology，china agricultural university，beijing 100094，china college of animal science and technology，china agricultural university，beijing 100094，china college of animal science and technology，Northeast agricultural university，Harbin 150030，china

Fattiness is an important parameter to estimate meat quality, which has high heritability. In this experiment, F2 chickens derived from Broilers crossing to Silky were used to study the effect of extracellular fatty acid binding protein (EX-FABP) gene on abdominal fat accumulation. Five exons and partial introns of the gene were amplified by six pairs of primers, and then single nucleotide polymorphisms (SNPs) were detected by single strand conformation polymorphism (SSCP) and subsequently confirmed by sequencing. There were four nucleotides variations found, T-C at 659, C-T at 924, CA-TG at 968 and C-T at 1985 respectively. The result of least square analysis suggests that the chickens with HH genotype defined by the third pair of primers have a lower abdominal fat weight and abdominal fat percentage than the chickens with other genotypes (GG, GI, GH and II). It implies that extracellular fatty acid binding protein gene could be a candidate locus or linked to a major gene that significantly affects abd

关键词： abdominal fat percentage EX-FABPs SNPs.

Chicken QTL mapping by multiplex PCR

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Progress in Natural science:Materials International 2002年第1期 75-78页

作者： HUANG Yinhua, HU Xiaoxiang, deng xuemei, XU Weizhuo, li Ning , FENG Jidong, SUN Han and WU Changxin( State Key Laboratory for Agribiotechnology, china agricultural university, beijing 100094, china college of animal science and technology, Jiangxi agricultural university, Nanchang 330045, china college of animal science and technology, china agricultural university, beijing 100094, china) State Key Laboratory for Agribiotechnology，china agricultural university，beijing 100094，china college of animal science and technology，Jiangxi agricultural university，Nanchang 330045，china State Key Laboratory for Agribiotechnology，china agricultural university，beijing 100094，china college of animal science and technology China Agricultural University，Beijing 100094，China college of animal science and technology，Jiangxi agricultural university，Nanchang 330045，china

To facilitate rapid determination of the chromosomal location of quantitative trait loci, the current approaches to gene mapping are improved using a multiplex PCR technique. The high-throughput linkage analysis method described here allows selection of 178 from 328 microsateilite markers through the multiplex PCR method combined with the semi-automatic fluorescence-labeled DNA analysis technology. Those polymorphism markers are distributed on 23 autosomes and one sex chromosome (chromosome Z), covering 3080cM genetic distance. The average marker density is 18cM, dispersed into 30 different sets. These selected polymorphism microsateilite markers segregate with the family members, following the Mendel's heritage laws, and are very useful for chicken linkage map analysis as well as for the research on some important economic quantitative characters of chicken.

关键词： chicken genomic scanning multiplex PCR microsatellite marker genotype.

Insulin-like growth factor 2 as a candidate gene influencing growth and carcass traits and its bialleleic expression in chicken

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science china(life sciences) 2005年第2期48卷 187-194页

作者： WANG Genyu1,YAN Bingxue1,deng xuemei2,li Changlü2,HU Xiaoxiang1 & li Ning1 1.State Key Laboratory for Agrobiotechnology,china agricultural university,beijing 100094,china 2.Department of animal Genetics and Breeding,college of animal science and technology,china agricultural university,beijing 100094,china State Key Laboratory for Agrobiotechnology China Agricultural University Beijing 100094 China Department of animal Genetics and Breeding College of Animal Science and Technology China Agricultural University Beijing 100094 China

We have identified DNA polymorphisms in the gene of insulin-like growth factor 2 by PCR-SSCP in a resource population, which was generated by Silky reciprocally crossing to Broilers. A C→G mutation was detected in the exon 2 (at position 71) by sequencing. This single nucleotide polymorphism (SNP) was found to be associated with production traits. Chicken with BB genotype showed more chest angle width but less 3 week body weight and glandular stom- ach weight than chicken with AA genotype (Pliver and 90-day-old liver, kidney, heart, and muscle of both heterozygote AB and BA chickens. Therefore, IGF2 was not an imprinting gene in chicken. The different genetic effects between the het- erozygote AB and BA remain to be elucidated.

关键词： chicken, IGF2, single nucleotide polymorphism, genome imprinting.

The single nucleotide polymorphisms of the chicken myostatin gene are associated with skeletal muscle and adipose growth

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science china(life sciences) 2004年第1期47卷 25-30页

作者： GU Zhiliang, ZHU Dahai, li Ming, li Hui, deng xuemei & WU ChangxinMolecular and Cellular Developmental Biology Laboratory, Harbin Institute of technology, Harbin 150001, china college of animal science & technology, Northeast agricultural university, Harbin 150030, china National Laboratories for Agribiotechnology, china agricultural university, beijing 100094, china college of animal science & technology, china agricultural university, beijing 100094, china Department of life science and technology, Changshu Institute of technology, Changshu 215500, china Department of Biochemistry and Molecular Biology, National Laboratory of Medical Molecular Biology, Peking Union Medical college, beijing 100005, china

Myostatin, a new member of the TGF-p superfamily, is predominantly expressed in skeletal muscle cells and functions as a negative regulator of skeletal muscle growth in animals. Recently, we have reported three single nucleotide polymorphisms (SNPs) in the chicken my-ostatin gene. Herein, we investigate the association of those SNPs with the production traits in a F2 chicken line derived from Broilers crossing to Silky with the least square analysis. The results show that the BB and AA genotypes are strongly associated with abdominal fat weight (AFW), abdominal fat percentage (AFP), and birth weight (BW) (P ling skeletal muscle growth and differentiation, but also may be involved in regulation of adipose growth in chicken.

关键词： myostatin gene, SNPs, F2 chicken population, abdominal fat, breast muscle weight.

Single nucleotide polymorphism analysis on melanocortin receptor 1 (MC1R) of Chinese native pig

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science china(life sciences) 2004年第3期47卷 287-292页

作者： SHI Kerong1,2, WANG Aiguo1, li Ning2 & deng xuemei1 1. college of animal science and technology, china agricultural university, beijing 100094, china 2. National Laboratory for Agribiotechnology of china agricultural university, beijing 100094, china college of animal science and technology China Agricultural University Beijing 100094 China National Laboratory for Agribiotechnology of china agricultural university Beijing 100094 China college of animal science and technology China Agricultural University Beijing 100094 China National Laboratory for Agribiotechnology of china agricultural university Beijing 100094 China

Melanocortin receptor 1 (MC1R) gene, one of the important candidate genes for coat color trait, was used to analyze the single nucleotide polymorphism (SNP) in Chinese native pig breeds by PCR-single strand conformation polymorphism (PCR-SSCP). The study had also taken 3 imported pig breeds as control. The results showed that the three mutations G284A, T309C and T364C found in Chinese native pigs were consistent to the mutation found in the European Large Black individuals. However, 68CC or C492T and G728A were only found in the imported individuals, which were obviously different from the Chinese native pigs. Accordingly, we presumed that the coat colors of Chinese native pigs belonged to dominant black color sys-tem, which was completely distinct to that of imported pig breeds. Thus it was implied that MC1R gene was not the principal factor affecting the coat color differences of Chinese native pig breeds, but could be used to trace the molecular evolution of pig breeds.

关键词： pig, coat color, MC1R gene, polymorphism.

The single nucleotide polymorphisms of chicken melanocortin-4 receptor(MC4R)gene and their association analysis with carcass traits

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science china(life sciences) 2006年第6期49卷 560-566页

作者： QIU xuemei1,2,li Ning1,deng xuemei3,ZHAO Xingbo3,MENG Qingyong1 & WANG Xiuli2 1. State Key Laboratory for Agrobiotechnology,china agricultural university,beijing 100094,china 2. college of life science and technology,Dalian Fisheries university,Dalian,116023,china 3. college of animal science and technology,china agricultural university,beijing 100094,china State Key Laboratory for Agrobiotechnology China Agricultural University Beijing 100094 China college of life science and technology Dalian Fisheries University Dalian 116023 China State Key Laboratory for Agrobiotechnology China Agricultural University Beijing 100094 China college of animal science and technology China Agricultural University Beijing 100094 China college of life science and technology Dalian Fisheries University Dalian 116023 China

Mutations of the melanocortin-4 receptor (MC4R) gene are associated with the appetite, obesity; growth in pig, mice; human. But little is known about the function of chicken MC4R gene. In this study, F2 chicken resource population derived from broilers crossing to Silky was screened for the polymorphisms of the MC4R gene using PCR-single strand conformation polymorphism (PCR-SSCP); DNA sequencing methods. Four single nucleotide polymorphisms (SNPs) sites were found. The mutation (C→T) in the 5′ regulation region of chicken MC4R gene results in one more NF-E2; cap transcription factor binding sites in the mutation allele than in the wild allele. One missense mutation (G→A) occurs in the coding region (61nt), which changes the glycine to arginine. Moreover, in the coding region there are 2 synonymous mutations, one G→T mutation at 315nt; one C→T mutation at 336nt. Least square analysis of the SNPs; carcass traits showed that BB, DD; FF genotypes are significantly associated with body weight, carcass weight (or half carcass weight),; leg muscle weight (P>0.05 or P>0.01). But no significant association between the genotypes; abdominal fat weight is found. The results present the evidence that the chicken MC4R gene can be selected as the major candidate gene for the carcass traits such as body weight; growth.

关键词： melanocortin-4 receptor, SNPs, chicken, carcass traits.

Single nucleotide polymorphisms in chicken lmbr1 gene were associated with chicken growth and carcass traits

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science china(life sciences) 2007年第1期50卷 62-69页

作者： HUANG YanQun1,2,3, DU XiaoHui2, deng xuemei4, QIU XiangPin2, WANG ChunKao4, CHEN Wen3, li Ning1 & WU ChangXin4 1 State Key Laboratory for Agrobiotechnology, china agricultural university, beijing 100094, china 2 college of animal science, Sichuan agricultural university, Ya’an 625014, china 3 college of animal Husbandry and Veterinary, Henan agricultural university, Zhengzhou 450002, china 4 college of animal science and technology, china agricultural university, beijing 100094, china State Key Laboratory for Agrobiotechnology China Agricultural University Beijing 100094 China college of animal science Sichuan Agricultural University Ya'an 625014 China college of animal Husbandry and Veterinary Henan Agricultural University Zhengzhou 450002 China college of animal science Sichuan Agricultural University Ya'an 625014 China college of animal science and technology China Agricultural University Beijing 100094 China college of animal Husbandry and Veterinary Henan Agricultural University Zhengzhou 450002 China State Key Laboratory for Agrobiotechnology China Agricultural University Beijing 100094 China

Lmbr1 is the key candidate gene controlling vertebrate limb development, but its effects on animal growth and carcass traits have never been reported. In this experiment, lmbr1 was taken as the candi-date gene affecting chicken growth and carcass traits. T/C and G/A mutations located in exon 16 and one A/C mutation located in intron 5 of chicken lmbr1 were detected from Silky, White Plymouth Rock broilers and their F2 crossing chickens by PCR-SSCP and sequencing methods. The analysis of vari-ance (ANOVA) results suggests that T/C polymorphism of exon 16 had significant association with eviscerated yield rate (EYR), gizzard rate (GR), shank and claw rate (SCR) and shank girth (SG); A/C polymorphism of intron 5 was significantly associated with SCR, liver rate and head-neck weight (HNW), while both sites had no significant association with other growth and carcass traits. These results demonstrate that lmbr1 gene could be a genetic locus or linked to a major gene significantly affecting these growth and carcass traits in chicken.

关键词： chicken lmbr1 SNPs shank and claw rate carcass traits

A microarray study of altered gene expression during melanoblasts migration in normal pigmented White Leghorn and hyperpigmented mutant Silky Fowl

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Frontiers of agricultural science and Engineering 2014年第4期1卷 299-306页

作者： Yulin li Deping HAN Junying li Dawn KOLTES xuemei deng Key Laboratory of animal Genetics and Breeding of the Ministry of Agriculture College of Animal Science and TechnologyChina Agricultural UniversityBeijing 100193China Iowa State university AmesIA 50011USA

Melanoblasts originating from neural crest cells can migrate through the mesenchyme of the developed embryo and give rise to *** the melanocytes that are confined to the integument in other vertebrates,melanocytes in Silky Fowl can reach the ventral regions of the embryos owing to differences in gene expression in the process of melanoblasts *** this study,we used microarray profiling to identify differences in gene expression between White Leghorn and Silky *** expression of 2517 microarray probes(P2)was observed in Silky Fowl compared to White *** filtration by cluster analysis,functional annotation and pathway analysis,eight differentially expressed genes were identified to be closely related to the development of ***,differences in expression of immune genes were also detected between Silky Fowl and White *** differentially expressed genes associated with melanocyte development were verified by q-PCR,and results were highly consistent with the microarray *** genes with significantly altered expression involved in melanoblast migration and development suggested that different microenvironments resulted in the abnormal melanoblast migration in Silky Fowl,although there were no big differences in melanoblast development between these two *** candidate genes discovered in this study are beneficial to understand the molecular mechanism of hyperpigmentation in Silky Fowl.

关键词： Silky Fowl White Leghorn melanoblast migration gene expression

鸡显性白羽基因PMEL17多态性及基因型鉴定方法

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中国家禽 2020年第9期42卷 12-18页

作者：陈建飞华国营李锦南李厚诚袁艺博邵天琦李俊英邓学梅中国农业大学动物科学技术学院北京100193

为建立显性白羽基因型检测的快捷方法,试验采用Sanger测序法扫描了白来航、寿光鸡和农大C系前黑素小体蛋白17(PMEL17)基因的全长并进行序列比对,共得到18个与显性白羽表型完全关联的标记。试验选择白来航PMEL17基因第5内含子上的22 bp... 详细信息

为建立显性白羽基因型检测的快捷方法,试验采用Sanger测序法扫描了白来航、寿光鸡和农大C系前黑素小体蛋白17(PMEL17)基因的全长并进行序列比对,共得到18个与显性白羽表型完全关联的标记。试验选择白来航PMEL17基因第5内含子上的22 bp插入以及第7外显子上的60 bp插入作为显性白羽基因型辅助鉴定的分子标记,并设计了特异性引物对。通过扩大群体和样本数量检测,证明该鉴定方法具有操作简便、结果清晰的优势,检测效力明显提高。研究提供了鸡显性白羽基因型检测的优化位点,为白羽鸡的分子育种提供了新的标记辅助鉴定技术。

关键词：鸡显性白羽 PMEL17 多态性检测标记辅助选择