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POTENTIATION EFFECT OF DIAZEPAM ON ACTH ANALGESIA IN THE MICE

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针刺研究 1989年第Z1期 117-118页

作者： li Haiti Zhang Wende department of physiology, third military medical university, chongqing

In the present study we injected diazepam, r-aminobutyric acid (GABA)receptor antagonist——picrotoxin and GABA synthesis inhibitor——isoniazid into the intraperitoneal (ip). Mice painful threshold and serotonin (5-HT)content in mice brain were measured by spectrofluorometric assay, the effectof diazepam on ACTH analgesia was obstraeted. The possible mechanisms ofdiazepam effect were studied.

关键词： ACTH POTENTIATION EFFECT OF DIAZEPAM ON ACTH ANALGESIA IN THE MICE

Influence of activities in arousal system on the excitability of principal neurons of entorhinal cortex and memory

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Influence of activities in arousal system on the excitabilit...

中国神经科学学会第九届全国学术会议暨第五次会员代表大会

作者： Yang li,Jianxia XIA,Xingshu CHEN,Jiali WANG,Jie YAN,Zhongxiang YAO,Zhian HU~* department of physiology,third military medical university,chongqing,400038,China

The entorhinal cortex(EC) has long been regarded as a bidirectional gateway between higher cortical areas and the ***(hypocretins) are wake-promoting neuropeptides which are mainly produced by the neurons within the lateral *** system includes two separate peptides orexin-A and orexin-B proteolytically derived from the same precursor protein and two specific G-protein-coupled receptors OX1R and *** the present study,we firstly investigated the electrophysiological effects of orexin-A on stellate neurons and pyramidal neurons in live brain slices of the EC using whole-cell patch-clamp recordings,and then observed signaling cascades triggering the ionic and synaptic mechanisms involved in *** results show as follow:Orexin-A depolarizes stellate and pyramidal neurons in the superficial layers of EC throμgh effects on K channels and nonselective cation *** excitatory action of orexin-A involves OX1Rs and PKC mediates both the inhibition of IK and activation of NSCC induced by ***-A enhances both excitatory and inhibitory synaptic transmissions in the superficial layers of EC via *** summury,the studies reported here provide evidence that the direct excitatory effects of orexin-A on principal neurons in the superficial layers of rat EC are mediated by the activation of OX1Rs and PKC signaling cascades,which inhibit sustained K current and activate *** addition, orexin-A enhances both excitatory and inhibitory synaptic transmissions in the EC via OX1Rs.

关键词： orexin entorhinal cortex learning and memory

Neuronal differentiation and synapse formation of embryonic stem cellderived precursors transplanted into the hippocampus of rats receiving Aβ（1-40） injection

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Neuronal differentiation and synapse formation of embryonic ...

中国神经科学学会第四次会员代表大会暨第八届全国学术会议

作者： Zhi-Fang li~1,Wei-Zhong SUN~1,Hong-liang YI~1,Xiao-Tang FAN~2,Hai-Wei XU~(1,*) 1 department of physiology,third military medical university,chongqing,400038,China 2 department of Developmental Biology,third military medical university,chongqing,400038,China

＜正＞Objective Advances in stem cell research have raised the hope and possibility for the therapy of neurodegenerative *** Alzheimer’s disease（AD） transgenic animal models,stem cell transplantation has been demonstrated to reverse behavioral *** recent study has demonstrated that embryonic stem（ES） cell-derived neural precursor cells improve memory dysfunction in rats with injections ofβ-amyloid peptide（1-40）（Aβ1-40） in the dorsal ***,the underlying mechanisms have remained unknown as most studies suggested that the observed behavioral improvements appeared to be related to structural reorganization such as specific neuronal differentiation and synapse *** present study was performed to test a murine ES cell-based approach in rats subjected to Aβ1 -40 injection into hippocampus dentate *** Efficacy of cell therapy regarding graft survival,neuronal yield and diversity,and synapse formation of the grafted cells were tested after transplanting ES cell-derived neural precursors into the hippocampus of adult rats with immunohistochemistry,quantum labeling and electric microscopy. Results Here,we show that grafted cells can survive,and differentiate with high yield into immunohistochemically mature glial cells and neurons of diverse *** importantly,transplanted cells demonstrate characteristics of proper synapse formation between host and grafted neural *** Our observations show that ES cell-based transplantation approach may be promising in treatment of AD.

关键词： synapse transplantation β-amyloid quantum dot embryonic stem cell

Orexin-A modulates the excitability of principal neurons in the superficial layers of rat entorhinal cortex

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Orexin-A modulates the excitability of principal neurons in ...

中国生理学会第23届全国会员代表大会暨生理学学术大会

作者： Yang li,Jia-Xiang XIONG,Jie YAN,Wei HUANG,Jian-Xia XIA,Zhi-An HU~* department of physiology,third military medical university,chongqing,400038,China

The entorhinal cortex(EC) has long been regarded as a bidirectional gateway between higher cortical areas and the ***(hypocretins) are wake-promoting neuropeptides which are mainly produced by the neurons within the lateral *** system includes two separate peptides orexin-A and orexin-B proteolytically derived from the same precursor protein and two specific G-protein-coupled receptors OX1R and *** the present study,we firstly investigated the electrophysiological effects of orexin-A on stellate neurons and pyramidal neurons in live brain slices of the EC using whole-cell patch-clamp recordings,and then observed signaling cascades triggering the ionic and synaptic mechanisms involved in *** results show as follow: Orexin-A depolarizes stellate and pyramidal neurons in the superficial layers of EC through effects on K channels and non-selective cation *** excitatory action of orexin-A involves OX1Rs and PKC mediates both the inhibition of IK and activation of NSCC induced by ***-A enhances both excitatory and inhibitory synaptic transmissions in the superficial layers of EC via *** summary,the studies reported here provide evidence that the direct excitatory effects of orexin-A on principal neurons in the superficial layers of rat EC are mediated by the activation of OX1Rs and PKC signaling cascades,which inhibit sustained K current and activate *** addition,orexin-A enhances both excitatory and inhibitory synaptic transmissions in the EC via OX1Rs.

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Anterior cingulate cortex substitutes for the impaired retrieval role of prelimbic cortex in trace eyeblink conditioning

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Anterior cingulate cortex substitutes for the impaired retri...

中国神经科学学会第九届全国学术会议暨第五次会员代表大会

作者： Guang-Yan WU~(1,2,3),Zheng-li FAN~2,Xuan li~2,Bing WU~2,Rui FAN~4,Jian-Feng SUI~(1,2,*) 1 department of physiology,College of Basic medical Sciences,third military medical university,chongqing 400038, China 2 Experimental Center of Basic Medicine,College of Basic medical Sciences,third military medical university, chongqing 400038,China 3 The Second Team of Cadet Brigade,third military medical university,chongqing 400038,China 4 department of Rehabilitation,the Second Affiliated Hospital,chongqing medical university,chongqing 400010, China

＜正＞Objective It has been suggested that the anterior cingulate cortex（ACC） and prelimbic cortex（PL） of medial prefrontal cortex（mPFC） have critical roles in the acquisition and retrieval（expression） processes of trace eyeblink conditioning（TEC）,*** was also reported that re-training after PL lesions following initial learning could recover the impaired behavioral *** on these results,we hypothesized that ACC substituted for the impaired retrieval role of PL in *** present study is to investigate（1） the hypothesis of TEC retrieval substitution and（2） the natural roles of ACC and PL in *** lidocaine hydrochloride（LH） or saline was microinfused into ACC,or PL,or both ACC and PL after daily pre-infusion session training following 8 days initial acquisition ***（1） Infusion of lidocaine into area 24 does not significantly affect TEC expression.（2） Area 24 infusions affect TEC acquisition as evidenced by a difference in pre-infusion and post-infusion performance between sham and lesion groups that develops over several infusion days.（3） Area 32 infusions have immediate effect on TEC expression.（4） The expression deficits with area 32 infusions fades over 5 infusion sessions,and（5） subsequent dual infusions into both regions produces immediate deficits that do not *** The results support our hypothesis of substitution and also indicate that reversible inactivation of ACC prevented the acquisition of TEC but did not affect its retrieval,whereas reversible inactivation of PL caused significant deficits in retrieval of TEC,but did not affect its acquisition.

关键词： guinea pig lidocaine hydrochloride medial prefrontal cortex retrieval substitution reversible inactivation trace conditioning

Targeted migration of engrafted neural precursor cells derived from ES cells and their differentiation in A β-injuried hippocampus of rats

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Targeted migration of engrafted neural precursor cells deriv...

中国神经科学学会第四次会员代表大会暨第七届全国学术会议

作者： Jun TANG,Hai-Wei XU~*,Xiao-Tang FAN,Zhi-Fang li,Da-Bing li,li YANG,Guang-Ji ZHOU department of physiology,third military medical university,chongqing,400038,China

＜正＞Neural stem cells（NSCs） could be applied for neuronal regeneration and structural brain repair only when they migrate within the CNS and reach the precise regions where neuronal replacement is *** the brain was injuried, NSCs in the hippocampus and subventricular zone（SVZ） of the adult changed their migratory route and moved toward the injury *** present study,we observed the migration and differentiation of neural precursor cells（NPCs） derived from murine embryonic stem（ES） cells when transplanted into the A β-injuried hippocampus of *** between migration distance and differentiation ratio of the engrafted NPCs was also *** ES cell line MESPU35,which expressing enhanced green fluorescent protein（EGFP）,was differentiated into nestin-positive NPCs with modified serumfree methods before *** migration distance was increased about 5 times 12 weeks *** differentiation ratio to GFAP-positive cells of migratory NPCs kept rising from about 30.41±1.45%in 4 weeks to 49.25±1.23%in 16 weeks,and differentiation ratio to NF200-positive cells was increased from 16.68±0.95%in 4 weeks to 27.94±1.21%in 16 ***,the percentage of migratory GFAP-positive cells distributed at the ipsilateral of Aβplaques were increased from 60.2%to 81.3%,and the percentage of migratory NF200-positive cells localized at the ipsilateral was increased from 61.3%to 84.1%.It showed that there was a significant positive linear correlation between migration distance and the neural differentiation ratio（correlation coefficient,r=0.991）,a significant positive linear correlation between migration distance and differentiation ratio to astrocyte（correlation coefficient,r = 0.953）.Our findings suggested that engrafted NPCs migrated targetedly to the A β-injured site and differentiated into neurons and astrocyte.

关键词： Embryonic stem cells β-amyloid peptide Cell transplantation Differentiation Migration Rat

5-羟色胺和生长抑素对P物质及慢痛引起的脊髓背角神经元电活动的影响

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针刺研究 1996年第3期21卷 27-31页

作者：阮怀珍李希成蔡文琴第三军医大学生理教研室重庆630038 第三军医大学组胚教研室

本研究用多管微电极细胞外记录和离子微电泳方法，以Wistar大鼠为实验对象，观察了P物质（SP）对脊髓背角神经元自发放电和慢性伤害性痛放电的影响以及与5-羟色胺（5－HT）和生长抑素（SOM）的关系。结果表明：（1）电泳SP可使脊髓背角... 详细信息

本研究用多管微电极细胞外记录和离子微电泳方法，以Wistar大鼠为实验对象，观察了P物质（SP）对脊髓背角神经元自发放电和慢性伤害性痛放电的影响以及与5-羟色胺（5－HT）和生长抑素（SOM）的关系。结果表明：（1）电泳SP可使脊髓背角神经元单位放电增多。（2）电泳SP又可抑制福尔马林（Formalin）引起的神经元慢性伤害性痛放电。（3）电泳5－HT和SOM可抑制SP及慢痛诱发的脊髓背角神经元放电。上述结果提示：在痛觉调制过程中，脊髓内SP起着传递痛觉信息和镇痛的双重作用，5－HT和SOM可通过抑制SP的效应，参与痛觉调制。

关键词： P物质 5-羟色胺生长抑素脊髓背角神经元痛觉

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吗啡对鞘内注射P物质引起的痛阈和c-fos表达的影响

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针刺研究 1996年第1期21卷 65-69页

作者：阮怀珍李希成黎海蒂第三军医大学生理教研室重庆630038

本文采用测痛和免疫组织化学法，观察到大鼠鞘内注射（it）P物质（SP）所引起痛反应与剂量有关，小剂量（0．5μg，1μg）对痛阈无明显影响，大剂量（5μg，10μg）可使之降低，并可诱发脊髓c－fos的表达。it吗啡（10μg）不仅使痛阈提... 详细信息

本文采用测痛和免疫组织化学法，观察到大鼠鞘内注射（it）P物质（SP）所引起痛反应与剂量有关，小剂量（0．5μg，1μg）对痛阈无明显影响，大剂量（5μg，10μg）可使之降低，并可诱发脊髓c－fos的表达。it吗啡（10μg）不仅使痛阈提高，还可抑制itSP产生的痛反应及脊髓C－fos表达，此效应可被纳洛酮翻转。

关键词： P物质鞘内注射吗啡痛域原癌基因蛋白c-fos

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Severe burn-induced acute insulin resistance is mediated by transient hyperglycemia-triggered AGEs production

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Severe burn-induced acute insulin resistance is mediated by ...

第九届全国钙信号和细胞功能研讨会

作者： Xing Zhang,Xiaoqing Cai,Jie Xu,Xu li,Yuan Zhang,Jia li,Feng Gao department of physiology,Fourth military medical university,Xi’an 710032,China

Hyperglycemia has been well recognized among patients suffering severe burns and is identified as an independent risk factor for adverse outcomes of critical ***, we investigated the dynamic profile of acute insulin resistance(AIR)following severe burns(third-degree,comprising 40%of total body surface area)in *** hyperglycemia with two peaks at 30 min(increased 13%)and 3 h(increased 35%)

关键词： Animal=rat Method=burn injury Topic=acute insulin resistance