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Cross-breeding of Different Geological Populations of Scallop, Chlamys farreri

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High Technology Letters 2002年第3期8卷 28-31页

作者：吴谡琦 Sun Xiuqin Zhang Jinxing li taiwu Hong Xuguang First Institute of Oceanography SOA Qingdao 266061 P.R.China Ningbo University Ningbo 315211 P.R.China

Wild populations of Chlamys farreri derived from Japan and China are used as parents in a cross breeding experiment conducted in Zhangzi Island of liaoning Province in the early May of 2000. Four different first filial generations of the crossbreeding, namely JC, CJ, CC and JJ, which represent different cross combinations of the parents, are differentiated from each other in some observed quantitative traits including survival rate, hatchery rate and attachment rate, etc. By ANOVA analysis, significant differences in growth rate are found among the four populations of the offspring after one year of culturing in Shandong Province. JJ population shows the fastest growth rate and has shell height of 46.1±0.71(SD)mm in average, whereas the other three populations, CC, CJ and JC, has the shell height of 43.1±0.42mm, 41.5±0.48mm and 37.2 ±0.31mm, respectively. No heterosis is detected in the experiment.

关键词： cross breeding scallop Chlamys farreri

MiRNA-133 modulates TLR-triggered immune response in the sea cucumber Apostichopus japonicus via targeting of IRAK-1

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MiRNA-133 modulates TLR-triggered immune response in the sea...

“全球变化下的海洋与湖沼生态安全”学术交流会

作者： Meng LU Pengjuan ZHANG Chenghua li Weiwei ZHANG ChunHua JIN taiwu li School of Marine Sciences Ningbo University

Many organisms have adopted a conserved Toll-like receptor（TLR） signaling pathway in their innate immune system to defend against the invasion of pathogenic microorganisms. However, the underlying regulatory mechanism is not well understood, especially in invertebrates. In this study, we explored the potential roles of miRNA-133 in regulating TLR pathways in the sea cucumber Apostichopus japonicus. A target screening of RNA-seq data successfully identified interleukin-1 receptor-associated kinases（AjIRAK-1） as one of putative targets of miR-133. This result was further validated by the negative expression profiles in Vibrio splendidus-challenged coelomocytes and LPS-exposed cell culture. Using a dual-luciferase reporter fused to the 3’UTR of wild type or mutant AjIRAK-1 and the mutant, we found a 52.9% reduction in the luciferase activity in transfected HEK293 cells（p＜0.01） when compared to controls. Co-infection with miR-133 mimics or specific siRNA of AjIRAK-1 that significantly repressed the expression of AjIRAK-1 and its down-stream molecules, such as AjTRAF6, AjIκB and Ajp105, in primary coelomocytes. In contrast, a miR-133 inhibitor significantly increased the expression of these TLR-pathway members. All of the data provide firsthand evidence that miR-133 modulates TLR-based immune response through the targeting of AjIRAK-1 in this non-model invertebrates.

关键词： microRNA-133 Apostichopus japonicus TLR signal cascades Interleukin-1 receptor-associated kinases

Global metabolomic responses of sea cucumber Apostichopus japonicus to thermal stresses

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Global metabolomic responses of sea cucumber Apostichopus ja...

“全球变化下的海洋与湖沼生态安全”学术交流会

作者：邵铱娜李成华 Xiaocong CHEN Pengjuan ZHANG Ye li taiwu li Jinbo JIANG School of Marine Sciences Ningbo University Ningbo City College of Vocational Technology

Aestivation is an adaptation of the sea cucumber Apostichopus japonicus to high temperature. To date, however, little is known about the intrinsic mechanisms of A. japonicus to cope with the rising temperature in aquaculture at the metabolic level. In the present study, the global metabolomic responses were characterized in the muscles of A. japonicus at two threshold thermal points, 20 and 25 °C, by 1H NMR-based metabolomics. The results showed that both temperatures disturbed energy metabolism and induced osmotic regulation based on the metabolic biomarkers such as elevated threonine, alanine, arginine, glutamate, tyrosine, histidine, glycine, fumarate, glucose, AMP and decreased ATP, aspartate, hypotaurine, choline after exposure for 0 and 3 days. At 7 days thermal-stress, several metabolites were similarly altered including the increased glutamine, glycine, glucose, AMP, tyrosine, histidine and decreased ATP at 20 °C. Surprisingly, a large amount of amino acids（valine, leucine, isoleucine, threonine, arginine, glutamate, phenylalanine） markedly declined at 25 °C. In addition, the mRNA expression levels of HSP70 and ATP synthase as well as SOD activity and MDA content were also investigated, and their expression levels were significantly altered at different exposure conditions. Our present results provided new insights into thermal-stressed effects on A. japonicus at metabolic responses.

关键词： Apostichopus japonicus muscles metabolomics nuclear magnetic resonance thermal stress

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泥蚶遗传多样性的研究

高技术通讯 2005年第12期15卷 104-110页

作者：吕振明李太武苏秀榕宁波大学生命科学与生物工程学院宁波315211

采用垂直聚丙烯酰胺同工酶电泳技术对我国及周边海域的7个泥蚶群体的¨种同工酶、近28个位点的表型变异及遗传分化进行了研究,结果发现,绝大多数同工酶在7个群体之间谱型上都存在较大差异.遗传变异统计结果表明:7个泥蚶群体多态位点比例... 详细信息

采用垂直聚丙烯酰胺同工酶电泳技术对我国及周边海域的7个泥蚶群体的¨种同工酶、近28个位点的表型变异及遗传分化进行了研究,结果发现,绝大多数同工酶在7个群体之间谱型上都存在较大差异.遗传变异统计结果表明:7个泥蚶群体多态位点比例(P.99)和(P.95)分别在39.3%～53.6%和28.6%～44.4%之间;平均杂合度观测值在0.035～0.083之间,平均杂合度预期值在0.086～0.186之间;而平均位点等位基因数在1.786～2.381之间.比较了7个群体之间的遗传相似度和遗传距离表明,7个泥蚶群体明显地分为两大类群,第一个类群包括釜山、荣成、奉化、乐清、福鼎5个群体,它们的平均遗传距离是0.0072;第二个类群包括汕头和湛江两个群体,它们的遗传距离是0.0320;而这两个类群间的不同群体间的平均遗传距离为0.4279.因此这两个类群的分类学地位值得进一步探讨.

关键词：泥蚶遗传多样性垂直聚丙烯酰胺同工酶电泳技术中国

Actigraph传感器在青少年体育活动能量消耗测试中的应用

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电子测试 2014年第9X期25卷 136-137,120页

作者：李泰武陕西交通职业技术学院西安710018

体育活动对青少年的健康十分有帮助,它具有能够增强体质和骨质健康、降低心血管和代谢性疾病风险等功效,Actigrap传感器能够更加准确的评估青少年的体育活动能量消耗,本文结合Actigrap传感器的工作原理研究了Actigraph传感器在青少年体... 详细信息

体育活动对青少年的健康十分有帮助,它具有能够增强体质和骨质健康、降低心血管和代谢性疾病风险等功效,Actigrap传感器能够更加准确的评估青少年的体育活动能量消耗,本文结合Actigrap传感器的工作原理研究了Actigraph传感器在青少年体育活动能量消耗测试中的应用,为研究体育活动与健康的关系提供了依据。

关键词： Actigraph传感器体育活动能量消耗

Two classes of glutathione S-transferase genes with different response profiles to bacterial challenge in Venerupis philippinarum

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Two classes of glutathione S-transferase genes with differen...

中国动物学会·中国海洋湖沼学会贝类学分会第九次会员代表大会暨第十五次学术讨论会

作者： Chenghua li~(a*) Xiurong Su~(a*) Ye li~(ab),taiwu li~(ab) Chongjie Sun~a Tingting Zhou~a a Faculty of life Science and Biotechnology,Ningbo University,Ningbo b Ningbo City College of Vocational Technology,Ningbo

Glutathione S-transferase(GST) is major cytosolic detoxification enzymes involved in many pathological and physiological *** the present study,two classes of GSTs(VpGST-1 and VpGST-2) were cloned from Venerupis philippinarum haemocytes by cDNA library and RACE *** alignments and phylogenetic analysis together supported that they belonged to a new member of sigma and pi classes GSTs protein family,*** expression profiles of these two genes under Vibrio anguillarum challenge were investigated by quantitative *** bacterial challenge could significantly up-regulate the mRNA expression of both VpGST-1 and VpGST-2 with larger amplitude in VpGST-2,and the feedback speed for VpGST-2 was more rapid than that of *** differences in the response to bacterial challenge indicated that they were functional diversity and probably played cooperative roles in mediating the Vibrio challenge in clam.

关键词： Venerupis philippinarum Glutathione S-transferase Expression profile Vibrio anguillarum

STUDIES ON KARYOTYPES OF DIPLOID AND TRIPLOID HARD CLAM,Mercenaria mercenaria

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STUDIES ON KARYOTYPES OF DIPLOID AND TRIPLOID HARD CLAM,Merc...

第十九届国际动物学大会

作者： Zhihua lin Zhenming Lu Xueliang Cai Jun Fang and taiwu li Zhejiang Mariculture Research Institute 6-1 Hetongqiao Wenzhou Zhejiang 325005 China College of Biology and Biotechnology Ningbo University Ningbo Zhejian China

＜正＞Hard clam （Mercenaria mercenaria ） is a commercially important species naturally distributed along the American Atlantic coast. Hard clam is a favorite shellfish on U. S market. Because its high price in marke...

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鲍的系统进化分析

海洋湖沼通报 2008年第2期 172-178页

作者：刘慧慧李太武苏秀榕浙江海洋学院海洋科学学院浙江舟山316004 宁波大学生命科学与生物工程学院浙江宁波315211

以鲍精子识别蛋白(lysin)为分子标记,通过对比已知的28种鲍lysin的氨基酸序列来研究鲍的系统发育特征。结果显示,28种鲍lysin的氨基酸序列存在很强的保守性特征,但在NH2-末端区域、适度变化的羧基末端、以及一些链内区域,氨基酸序列仍... 详细信息

以鲍精子识别蛋白(lysin)为分子标记,通过对比已知的28种鲍lysin的氨基酸序列来研究鲍的系统发育特征。结果显示,28种鲍lysin的氨基酸序列存在很强的保守性特征,但在NH2-末端区域、适度变化的羧基末端、以及一些链内区域,氨基酸序列仍存在着高度多态性。造成上述现象的最主要原因是各种鲍所生存的环境条件控制了其进化方向,各种鲍因为精子识别蛋白序列上的差异性而成为具有生殖隔离特征的不同种,又因为其中的若干种所处的生态环境相似而造成其分子序列上的某些相似性。

关键词：鲍精子识别蛋白系统进化

原油微生物群落构成多样性及降解菌DYL-1降解原油的研究

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生物技术通报 2011年第2期27卷 163-168页

作者：王中华李太武苏秀榕秦松李晔宁波大学生命科学与生物工程学院宁波315211 中国科学院烟台海岸带可持续发展研究所烟台264003

为分离得到高效原油降解菌,直接向原油中加入营养物刺激,培养一段时间后原油乳化降解,傅里叶红外光谱显示,2957cm-1、723cm-1处吸收峰消失;2855cm-1、1377cm-1处吸收峰减弱;880cm-1以及800cm-1吸收峰几乎消失,表明原油发生降解,效果明... 详细信息

为分离得到高效原油降解菌,直接向原油中加入营养物刺激,培养一段时间后原油乳化降解,傅里叶红外光谱显示,2957cm-1、723cm-1处吸收峰消失;2855cm-1、1377cm-1处吸收峰减弱;880cm-1以及800cm-1吸收峰几乎消失,表明原油发生降解,效果明显。同时分离得到一株降解菌,经分子鉴定为芽孢杆菌属(Bacillus sp.),红外光谱法和紫外吸收法分析表明其具有较强的降解原油烃的能力。根据传统分子生物学的方法,构建原油菌16S rDNA克隆文库,限制性酶切片段长度多态性(Restriction Fragment Length Polymorphism,RFLP)分析了原油中的细菌多样性。结果表明此方法有效地评估了原油中的细菌群落和多样性。

关键词：原油红外光谱 16S rDNA 生物降解 PCR