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基于拉曼技术的单细胞生长检测方法

生物化学与生物物理进展 2023年第6期50卷 1489-1496页

作者：李新立张欣雨杨强李肃义吉林大学仪器科学与电气工程学院长春130061

目的单细胞生长检测可以更加科学地揭示微生物代谢变化的规律,为后期微生物工程应用提供指导。针对微生物生长应用于食品安全期和最佳食用期的精准检测问题,本文提出一种基于拉曼技术的单细胞生长检测方法。方法首先,通过同步培养实验... 详细信息

目的单细胞生长检测可以更加科学地揭示微生物代谢变化的规律,为后期微生物工程应用提供指导。针对微生物生长应用于食品安全期和最佳食用期的精准检测问题,本文提出一种基于拉曼技术的单细胞生长检测方法。方法首先,通过同步培养实验采集了枯草芽孢杆菌两个批次共900个单细胞拉曼光谱(SCRS)数据,其中600个用于训练和测试,另一批次300个用于模型验证。其次,基于主成分分析的特征关系矩阵,提出CP-SP特征评估方法以筛选SCRS特征用于模型检测。再基于XGBoost构建检测模型,并应用网格搜索和交叉验证对检测模型进行调优。最后,应用混淆矩阵、ROC曲线评估模型对细胞滞后期、对数期和稳定期的检测准确率、敏感性和特异性。结果选用CP-SP筛选的第一、第二和第四主成分较特征贡献率前3个主成分的分类性能提高了3.1%,调优后的细胞生长检测模型测试准确率为96.0%,验证准确率为92.3%。结论基于拉曼技术的单细胞生长检测方法能准确识别单细胞生长状态且具有较高的泛化能力,可为食品安全和保鲜制定精准调控机制提供科学指导。

关键词：单细胞拉曼光谱细胞生长食品安全和保鲜特征关系矩阵 XGBoost

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Quantum public-key crypto via EPR pairs

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Science China(Physics,Mechanics & Astronomy) 2025年第1期Mechanics & Astronomy) .卷 58-63页

作者： Xiang-Qun Fu Hong-Wei li Jian-Hong Shi Tan li Wan-su Bao Henan Key Laboratory of Quantum Information and Cryptography Information Engineering University Synergetic Innovation Center of Quantum Information and Quantum Physics University of Science and Technology of China

With the development of quantum computing technology, quantum public-key cryptography is gradually becoming an alternative to the existing classical public-key cryptography. This paper designs a quantum trapdoor one-way function via EPR pairs and quantum measurements. Based on this, a new quantum public-key cryptosystem is presented, which offers forward security,and can resist the chosen-plaintext attack and chosen-ciphertext attack. Compared with the existing quantum public-key cryptos,eavesdropping can be automatically detected in this new quantum public-key cryptosystem under a necessary condition, which is also detailed in the paper.

关键词： quantum public-key crypto trapdoor one-way function EPR pairs

Effect of Progressive substitution of Cement and lime by Powdered Shells Used as a Curing Agent for Dredged Soil in a Port Area

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Journal of Wuhan University of Technology(Materials Science) 2025年第1期40卷 88-102页

作者： suN Yang WANG Faxin su Yaying li yiwei HE Feng College of Harbour Coastal and Offshore EngineeringHohai UniversityNanjing 210098China Huai'an Research Institute Hohai UniversityHuai'an 223025China Jiangsu Fangyang Industrial Investment Corp.Ltd. Lianyungang 210098China

This study aimed to address the challenges of solid waste utilization,cost reduction,and carbon reduction in the treatment of deep-dredged soil at Xuwei Port in lianyungang city of *** research in this area was ***,a curing agent made from powdered shells was used to solidify the dredged soil in *** employed laboratory orthogonal tests to investigate the physical and mechanical properties of the powdered shell-based curing *** was collected by conducting experiments to assess the role of powdered shells in the curing process and to determine the optimal ratios of powdered shells to solidified soil for different *** development of strength in solidified soil was studied in both seawater and pure water *** study revealed that the strength of the solidified soil was influenced by the substitution rate of powdered shells and their interaction with *** cement content had a positive effect on *** high-strength solidified soil,the recommended ratio of wet soil:cement:lime:powdered shells were 100:16:4:4,while for low-strength solidified soil,the recommended ratio was 100:5.4:2.4:***,under appropriate conditions,improved short-term strength by promoting the formation of expansive ettringite minerals that contributed to cementation and *** findings suggest that the combination of cement and powdered shells is synergistic,positively affecting the strength of solidified *** recommended ratios provide practical guidance for achieving desired strength levels while considering factors such as cost and carbon *** role of seawater in enhancing short-term strength through crystal formation is noteworthy and can be advantageous for certain *** conclusion,this research demonstrates the potential of using a powdered shell-based curing agent for solidifying dredged soil in an environmentally friendly and cost-effective *** recommended ratios for different str

关键词： carbon reduction and solidification dredged soil abandoned shells orthogonal test chemical additives(lime and cement) unconfined compressive strength(UCS)

Magneto‑Dielectric Synergy and Multiscale Hierarchical Structure Design Enable Flexible Multipurpose Microwave Absorption and Infrared Stealth Compatibility

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Nano-Micro Letters 2025年第2期17卷 401-416页

作者： Chen li Leilei liang Baoshan Zhang yi Yang Guangbin Ji School of Electronic Science and Engineering Nanjing UniversityNanjing 210093People’s Republic of China College of Materials Science and Technology Nanjing University of Aeronautics and AstronauticsNanjing 210016People’s Republic of China

Developing advanced stealth devices to cope with radar-infrared(IR)fusion detection and diverse application scenarios is increasingly demanded,which faces significant challenges due to conflicting microwave and IR cloaking mechanisms and functional integration ***,we propose a multiscale hierarchical structure design,integrating wrinkled MXene IR shielding layer and flexible Fe_(3)O_(4)@C/PDMS microwave absorption *** top wrinkled MXene layer induces the intensive diffuse reflection effect,shielding IR radiation signals while allowing microwave to pass ***,the permeable microwaves are assimilated into the bottom Fe_(3)O_(4)@C/PDMS layer via strong magneto-electric *** theoretical and experimental optimization,the assembled stealth devices realize a near-perfect stealth capability in both X-band(8–12 GHz)and long-wave infrared(8–14μm)wavelength ***,it delivers a radar cross-section reduction of−20 dB m^(2),a large apparent temperature modulation range(ΔT=70℃),and a low average IR emissivity of ***,the optimal device demonstrates exceptional curved surface conformability,self-cleaning capability(contact angle≈129°),and abrasion resistance(recovery time≈5 s).This design strategy promotes the development of multispectral stealth technology and reinforces its applicability and durability in complex and hostile environments.

关键词： Microwave absorption Radar-infrared compatible stealth Wrinkled MXene Magneto-dielectric synergy Multifunction

Malicious Document Detection Based on GGE Visualization

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Computers, Materials & Continua 2025年第1期82卷 1233-1254页

作者： Youhe Wang yi sun Yujie li Chuanqi Zhou Henan Province Key Laboratory of Information Security Information Engineering UniversityZhengzhou450000China

With the development of anti-virus technology,malicious documents have gradually become the main pathway of Advanced Persistent Threat(APT)attacks,therefore,the development of effective malicious document classifiers has become particularly ***,detection methods based on document structure and behavioral features encounter challenges in feature engineering,these methods not only have limited accuracy,but also consume large resources,and usually can only detect documents in specific formats,which lacks versatility and *** address such problems,this paper proposes a novel malicious document detection method-visualizing documents as GGE images(Grayscale,Grayscale matrix,Entropy).The GGE method visualizes the original byte sequence of the malicious document as a grayscale image,the information entropy sequence of the document as an entropy image,and at the same time,the grayscale level co-occurrence matrix and the texture and spatial information stored in it are converted into grayscale matrix image,and fuses the three types of images to get the GGE color *** Convolutional Block Attention Module-EfficientNet-B0(CBAM-EfficientNet-B0)model is then used for classification,combining transfer learning and applying the pre-trained model on the ImageNet dataset to the feature extraction process of GGE *** shown in the experimental results,the GGE method has superior performance compared with other methods,which is suitable for detecting malicious documents in different formats,and achieves an accuracy of 99.44%and 97.39%on Portable Document Format(PDF)and office datasets,respectively,and consumes less time during the detection process,which can be effectively applied to the task of detecting malicious documents in real-time.

关键词： Malicious document visualization EfficientNet-B0 convolutional block attention module GGE image

基于Brusselator模型的我国企业技术获取系统耗散结构研究

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科技进步与对策 2016年第20期33卷 91-96页

作者：李新李柏洲苏屹东北林业大学经济管理学院黑龙江哈尔滨150040 哈尔滨工程大学经济管理学院黑龙江哈尔滨150001

企业技术获取,尤其是外部技术获取是企业参与到"多方协同的新型创业创新机制"中来的具体体现形式。企业技术获取系统耗散结构的存在性是开展企业技术获取研究和官产学研技术合作研究的前提基础。从理论上分析了企业技术获取系统耗散结... 详细信息

企业技术获取,尤其是外部技术获取是企业参与到"多方协同的新型创业创新机制"中来的具体体现形式。企业技术获取系统耗散结构的存在性是开展企业技术获取研究和官产学研技术合作研究的前提基础。从理论上分析了企业技术获取系统耗散结构存在的合理性,并进一步借助Brusselator模型的经济学转译对我国企业技术获取系统耗散结构进行了实证检验。研究发现:我国企业能够开放自身组织边界实现外部技术的获取与二次创新,企业技术获取系统耗散结构是存在的;通过对Brusselator势函数的进一步分析与解读,解释了我国企业技术获取系统耗散结构的存在性和技术后发企业技术赶超得以实现的原因。在我国经济技术迅速发展、原始创新重要性愈发凸显的重要历史时期,我国企业应在技术交流合作和外部技术二次创新中消化吸收先进外部技术,逐步提升自主R&D水平,尤其是技术原创能力。

关键词：耗散结构 Brusselator模型技术获取势函数

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食管癌药物治疗临床研究进展

中国癌症杂志 2009年第3期19卷 228-233页

作者：蒋苏豫李苏宜东南大学附属中大医院临床肿瘤中心江苏南京210009

食管癌药物治疗可以显著提高有效性、延长患者生存期,化疗、放疗与手术联合是目前治疗食管癌的主要综合治疗模式。本文就食管癌药物治疗临床研究文献进行复习和思考。依据近年来临床研究新证据,分别就新型化疗药物治疗食管癌在综合治疗... 详细信息

食管癌药物治疗可以显著提高有效性、延长患者生存期,化疗、放疗与手术联合是目前治疗食管癌的主要综合治疗模式。本文就食管癌药物治疗临床研究文献进行复习和思考。依据近年来临床研究新证据,分别就新型化疗药物治疗食管癌在综合治疗中扮演不同角色时的有效性和安全性进行系统综述。文章还初步探讨了应用新靶点药物治疗食管癌的价值。

关键词：食管癌药物治疗综合治疗

奈达铂联合顺铂对人食管癌细胞株(Eca-109)抑制作用机制的研究

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实用肿瘤杂志 2010年第2期25卷 150-155页

作者：苏翔宇李苏宜刘琳东南大学附属中大医院肿瘤科江苏南京210009

目的研究奈达铂(NDP)联合顺铂(DDP)对人食管癌细胞株Eca-109的增殖及凋亡的影响,并探讨其机制。方法采用MTT法检测NDP联合DDP对Eca-109细胞的增殖抑制率,中效原理法判断联合用药的相互作用;采用RT-PCR和蛋白印迹法分析Ki-67及Bax表达的... 详细信息

目的研究奈达铂(NDP)联合顺铂(DDP)对人食管癌细胞株Eca-109的增殖及凋亡的影响,并探讨其机制。方法采用MTT法检测NDP联合DDP对Eca-109细胞的增殖抑制率,中效原理法判断联合用药的相互作用;采用RT-PCR和蛋白印迹法分析Ki-67及Bax表达的变化。结果NDP、DDP均明显抑制Eca-109细胞的生长,且呈剂量依赖性;两者联合使用低浓度时呈拮抗效应,高浓度时呈协同效应;IC50浓度的NDP、DDP和1/2IC50(NDP)+1/2IC50(DDP)对Eca-109细胞的抑制率分别为(41.9±4.1)%、(47.4±2.9)%和(52.5±0.9)%;与单独用药组比较,联合用药组Ki-67基因(蛋白)表达显著降低,而Bax基因(蛋白)表达显著增高。结论1/2IC50浓度NDP和DDP联合应用与两药IC50浓度单独应用相比,对Eca-109细胞增殖的抑制作用和诱导凋亡能力均有明显增强。

关键词：食管肿瘤/药物疗法顺铂/投药和剂量细胞增殖/药物作用细胞凋亡/药物作用有机铂化合物/治疗应用抗肿瘤联合化疗方案/治疗应用

Research of E-Commerce System Based on EJB for Manufacture Enterprise

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厦门大学学报（自然科学版） 2002年第S1期41卷 286-287页

作者： WEI Zhao-wen 1, li Yong 2, su yi-dan 1 (1. Computer & Information Engineering College, Guangxi University, N anning 530004, China 2. Business Administration College, Chongqing University, Chongqing 400044, Chi na)

During the popular and development in Internet, mor e and more EC systems are running on Internet. Manufacture enterprise establishes EC system, integrates the systems between enterprises replace the traditional MI S system. The manufacture enterprise will face some difficulties when it begins to establish EC system. In order to solve the difficulties, the EC system not on ly includes the functions which in general EC system, but also integrates ERP sy stem, manages the enterprise from three aspects: relationship between manufactur er and customers, enterprise resources, and supplying chain, and gives the manuf acturer more intelligent information. As the departments of enterprise exchange data very frequently, connect the sellers and suppliers by using Internet, the t raditional C/S architecture isn’t satisfied by present needs. It is the only wa y that uses Multi-tier architecture. EJB (Enterprise Java Bean) is a Multi-tier distributed architecture designed by suN company. The Enterprise JavaBeans architecture is a component architecture for the development and development of component-based distributed business app lications. Applications written using the Enterprise JavaBeans architecture are scalable, transactional, and multi-user secure. These applications may be writt en once, and then deployed on any server platform that supports the Enterprise J avaBeans specification. There are many superiorities using EJB when design compu ter system. The EC System for Manufacture Enterprise is based on EJB architecture. We define four EJB containers, namely, CRM component, POM component, Knowledge Management component and ERP component. CRM EJBs take the form of managing the customer- supplier relationship, reducing the number of contracts the manufacturer must ma ke with the end customers. POM EJBs take the form of order processing activities . These order taking, order fulfillment, and managing the logistics and transpor tation involved in the physical movement of the

关键词： E-Commerce EJB CRM ERP