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Journal of Integrative Agriculture 2012年第10期11卷 1691-1699页

作者： li Ming-gui liU Zhen-shan PAN Zeng-xiang LUO Hua XIE Zhuang li qi-fa College of Animal Science and Technology Nanjing Agricultural University

The H19 gene, which is imprinted with preferential expression from the maternal allele, was one of the first identified imprinting genes in mammals. Recent studies revealed that correct imprinting of the H19 gene plays a vital role in human spermatogenesis. To investigate whether imprinting defects were associated with the hybrid sterility of male cattle-yak, the methylation patterns of the H19 imprinting control region （ICR） and H19 mRNA expression in the testes of cattle-yak, yak, and cattle were examined. The results showed that the 3rd CCCTC-binding factor （CTCF） site of the H19 ICR was significantly hypomethylated in the testes of cattle-yak compared with yak or cattle. As expected, H19 was expressed at a significantly higher level in cattle-yak than in yak or cattle. These results suggest that imprinting defects of the CTCF- binding site in the HI9 ICR were possibly associated with disturbed spermatogenesis of male cattle-yak. Thus, we propose that disorders in H19 imprinting, resulting in an increased H19 mRNA expression, might contribute to the sterility of F1 male hybrids between cattle and yak.

关键词： cattle-yak male sterility H19 ICR CTCF-binding site mRNA expression DNA methylation

Relationship Between the mRNA Expression Level of TGF-β Receptor Genes in Tissues and Ovulation Rate in Hu Sheep

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Agricultural Sciences in China 2010年第11期9卷 1659-1666页

作者： li Er-lin XIE Xin-hua XU Ye-fen XIE Zhuang CHEN ling liU Hong-lin li qi-fa College of Animal Science and Technology Nanjing Agricultural University Nanjing 210095 P.R. China Breeding Sheep farm of Hu Sheep Stock Seed farm Suzhou 215200 P.R.China

Eight ewes of Hu sheep which bred multi-lamb were used as the high-fecundity group and the other eight ewes of Hu sheep which bred single lamb were used as the control group to investigate the relationship between the mRNA expression level of TGF-β receptor genes in tissues and ovulation rate in Hu sheep. Cloprostenol sodium was injected to make the synchronization of estrus treatment, then all ewes were slaughtered within 24-36 h after empathema and the ovaries were collected. Furthermore, the number of ovulation points was counted to determine ovulation rate for each sheep. Tissue expression analysis was conducted by RT-PCR for one ewe form the high-fecundity group and the relationship between the mRNA expression of genes encoding TGF-β receptors and ovulation rate was detected by real-time fluorescent quantitative PCR. The results showed that the relative expression level of TGF-flR I gene in the reproductive organ was significantly higher than in the lung and muscle （P 〈 0.01）, while relative expression level of TGF-βR H in reproductive organ was significantly higher than that of other tissues （P 〈 0.01）, indicating that these are highly expressed genes in the ovary. In addition, mRNA expression level of TGF-βR I and TGF-flRH in the ovaries of the high-fecundity group were significantly higher （P〈 0.01） and obviously higher （P= 0.011） than the control group, respectively. The mRNA expression level of TGF-βR I and TGF-βR H had a positive correlation with ovulation rate and the correlation coefficients were 0.562 （P〉 0.05） and 0.711 （P〈 0.05）, respectively. It is suggested that TGF-β receptors have close relationship with highfecundity in Hu sheep.

关键词： Hu sheep TGF-βR I TGF-βR H ovulation rate

Complete Sequence of Yak Mitochondrial D-loop Region and Its Taxonomic Status in Bovinae

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畜牧兽医学报 2009年第S1期40卷 18-22页

作者： li qi-fa li Yin-xia DONG li-yan ZHAO Xing-bo PAN Zeng-xiang li Ning XIE Zhuang Laboratory of Animal Reproductive Genetics and Molecular Evolution College of Animal Science and TechnologyNanjing Agricultural University State Key Laboratory for Agribiotechnology China Agricultural University

Primers based upon the mitochondrial genome sequences of Bos taurus were used to amplify and sequence the complete mitochondrial D-loop region of Jiulong yak(Poephagus grunniens).The results showed that the length of D-loop was 893 bp,with 87.4%homology to the Bos taurus D-loop sequence;there were 17 bp *** Ovis aries as an outgroup,the phylogeny of representative species of Bovinae(***,***,Bos taurus,Bos indicus,Bison bison,Bison bonasus,and Bubalus bubalis)was *** Bovinae,the sequence divergence between ***,*** and American bison(Bison bison)was 6.2%-6.8%,which was less than that of Bos taurus and Bos indicus within Bos(10.0%-11.3%).Phylogenetic analysis found that ***,*** and Bison bison clustered first of all,indicating there was higher genetic comparability among them than to that of *** data from paleontology,morphology,and molecular biology,the present analysis supports the argument that Poephagus grunniens and Poephagus mutus should be classified as a distinct genus in Bovinae,that is Poephagus.

关键词： Bovinae Poephagus yak(Poephagus grunniens) D-loop phylogenetic relationship taxonomic status

Developmental Changes of the LPL mRNA Expression and Its Effect on IMF Content in Sheep Muscle

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Agricultural Sciences in China 2008年第1期7卷 104-111页

作者： qiAO Yong HUANG Zhi-guo li qi-fa liU Zhen-shan DAI Rong PAN Zeng-xiang XIE Zhuang liU Hong-lin College of Animal Science and Technology Nanjing Agricultural University Nanjing 210095 P.R.China Sichuan University of Science ＆ Engineering Zigong 643000 P.R.China Xinjiang Reclamation Science lnst-itute Shihezi 832001 P.R.China

This study investigates the developmental changes of the lipoprotein lipase （LPL） mRNA level in sheep muscle and its effect on intramuscular fat （IMF） accumulation. Male Kazak and Xinjiang Merino sheep at 2-120 days old were selected. Six animals of each breed were slaughtered at 2, 30, 60, 90, and 120 days （only the Xinjiang Merino sheep at 120-day old were available） to collect samples from longissimus dorsi muscle for the purpose of determining the IMF content and extracting total RNA that was used to investigate the developmental changes of the LPL mRNA expression by real-time PCR. The results showed that in male Kazak sheep, the IMF content increased with the progress of development and there were significant differences （P〈0.05） between the age groups. However, there was no difference （P〉0.05） between age groups in Xinjiang Merino sheep. Furthermore, the IMF content of the male Kazak sheep was significantly higher （P 〈 0.01） than that of the Xinjiang Merino sheep aged from 30 to 90 days. The highest LPL mRNA expression appeared at day 2 and it was significantly higher than that of all other age groups （P 〈 0.01）, while animals at 60-day old had the lowest LPL mRNA expression in the male Kazak sheep. In Xinjiang Merino sheep, the highest one occurred at 30-day old （P〈0.01）, followed by a continuous decrease to the lowest level at 90-day old, and then it started to increase slightly. At 2 to 60-day old, the LPL mRNA expression was negatively correlated to the IMF content （r=-0.625, P 〈 0.05） in male Kazak sheep, but no such relationship was detected in the male Xinjiang Merino sheep.

关键词： sheep intramuscular fat (IMF) lipoprotein lipase (LPL) real-time PCR

SHIP2通过抑制PI3-K/Akt信号通路降低胰岛素敏感性

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生理科学进展 2010年第1期41卷 62-64页

作者：邹力李法琦重庆医科大学附属第一医院老年病科重庆400016

含SH2结构域的肌醇多磷酸5'-磷酸酶-2(Srchomology 2 domain containing insitol polyphos-phate5'-phosphatase2,SHIP2)负向调节磷脂酰肌醇3-激酶/蛋白激酶B依赖的胰岛素信号通路,降低胰岛素敏感性。SHIP2基因的变异能显著改变胰岛素敏感性。抑制内源性SHIP2蛋白或其基因表达,能够提高胰岛素敏感性,从而改善胰岛素抵抗,有可能成为研究胰岛素抵抗相关疾病发病机制和治疗药物开发的新途径。

关键词：肌醇多磷酸5’-磷酸酶基因表达胰岛素敏感性

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肠内营养在老年患者中的应用

中国全科医学 2012年第30期15卷 3544-3547页

作者：李巧李法琦重庆医科大学附属第一医院老年病科重庆市400016

营养支持分为肠内营养(EN)和肠外营养(PN),EN在维护肠黏膜屏障、正常肠道菌群、抑制各种前炎性递质的释放等方面优于PN。随着EN制剂及喂养方法的改进,目前EN得到广泛应用。营养状况是影响人体健康和衰老进程的重要组成部分,老年人因消... 详细信息

营养支持分为肠内营养(EN)和肠外营养(PN),EN在维护肠黏膜屏障、正常肠道菌群、抑制各种前炎性递质的释放等方面优于PN。随着EN制剂及喂养方法的改进,目前EN得到广泛应用。营养状况是影响人体健康和衰老进程的重要组成部分,老年人因消化吸收功能下降、常伴随多种基础疾病、进食差等原因,营养不良患病率较高。EN在老年人常见病、多发病中应用广泛。

关键词：营养疗法老年人肠内营养胃肠外营养

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HIFU治疗中的空化效应及其应用

中国医学影像技术 2007年第7期23卷 1097-1099页

作者：张婷李发琪重庆医科大学生物医学工程系医学超声工程研究所省部共建超声医学工程国家重点实验室重庆400016

超声空化效应是高强度聚焦超声(HIFU)治疗中的重要生物学效应之一,在形成凝固性坏死与提高治疗效率等方面起着关键性作用。因而在HIFU治疗中是否有空化效应参与,什么条件下发生,对热效应有怎样的影响,如何评价它的利弊及怎样去控制和利... 详细信息

超声空化效应是高强度聚焦超声(HIFU)治疗中的重要生物学效应之一,在形成凝固性坏死与提高治疗效率等方面起着关键性作用。因而在HIFU治疗中是否有空化效应参与,什么条件下发生,对热效应有怎样的影响,如何评价它的利弊及怎样去控制和利用它是研究者关注的问题,本文就这些问题进行综述。

关键词：高强度聚焦超声空化效应热效应

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半胱氨酸天冬氨酸蛋白酶与心血管疾病

中国临床康复 2005年第43期9卷 145-147页

作者：邱炯李法琦重庆医科大学附属第一医院心内科重庆市400016

目的:通过分析细胞凋亡的信号转导途径,探讨半胱氨酸天冬氨酸蛋白酶家族与心血管疾病的关系。资料来源:应用计算机检索美国NCBIPubmed数据库1980-01/200402关于细胞凋亡、半胱氨酸天冬氨酸蛋白酶与心血管疾病的文章,主要检索主题词包括... 详细信息

目的:通过分析细胞凋亡的信号转导途径,探讨半胱氨酸天冬氨酸蛋白酶家族与心血管疾病的关系。资料来源:应用计算机检索美国NCBIPubmed数据库1980-01/200402关于细胞凋亡、半胱氨酸天冬氨酸蛋白酶与心血管疾病的文章,主要检索主题词包括“caspases,cardivasculardiseases,apoptosis”,语言种类为English。资料选择:选取心血管疾病中,心肌细胞凋亡的信号转导途径的原创实验性文章以及2001年以来在权威期刊上发表的相关综述文章。纳入标准:①具有原创性,论点论据可靠的实验文章。②观点明确,分析全面的综述文章。③文献主体内容与本课题联系紧密的文章。排除标准:具有明显实验设计和结果错误的文章及观点模糊的综述。质量评价主要考察资料的真实性、实施过程是否严密。资料提炼:共检索到关于半胱氨酸天冬氨酸蛋白酶家族与心血管疾病的文献32篇,20篇文献符合纳入标准。排除的12篇文献均为重复性文章。资料综合:20篇文献分别阐明了在心血管疾病,如心肌梗死,心力衰竭高血压,肺动脉高压等的发生发展过程中,半胱氨酸天冬氨酸蛋白酶家族介导的凋亡发挥了重要的作用。应用半胱氨酸天冬氨酸蛋白酶抑制剂治疗心血管疾病的实验研究,已取得一定效果。结论:半胱氨酸天冬氨酸蛋白酶家族介导的凋亡,在多种心血管疾病的发生发展中,发挥着不可替代的作用,通过抑制其活性来对抗心肌细胞凋亡,从而保护心脏,有可能成为治疗心血管疾病的一项新策略。

关键词：心血管疾病半胱氨酸天冬氨酸蛋白酶细胞凋亡

高强度聚焦超声（HIFU）肿瘤热消融技术的关键问题简析

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中华肝胆外科杂志 2011年第2期17卷 175-176页

作者：李发琪重庆医科大学生物医学工程系超声医学工程重庆市市级重点实验室400016

目的 1988年，王智彪萌发了用高强度聚焦超声（highintensity focused ultrasound，HIFU）从体外对体内肿瘤进行非侵入切除的灵感。在之后的10年中，他和他的团队在该领域提出了“生物学焦域”、“超声治疗剂量学”、“组织声环境”等... 详细信息

目的 1988年，王智彪萌发了用高强度聚焦超声（highintensity focused ultrasound，HIFU）从体外对体内肿瘤进行非侵入切除的灵感。在之后的10年中，他和他的团队在该领域提出了“生物学焦域”、“超声治疗剂量学”、“组织声环境”等概念。在实时超声监控、治疗系统优化、远程医疗系统、临床方案等方面突破了相应的关键技术壁垒。将HIFU治疗技术成功运用于外科治疗，在国际上积累了数量最多的临床病例。在该领域的设备研制、临床应用及若干基础研究方面走在了世界前列。

关键词：高强度聚焦超声消融治疗肿瘤关键技术

Semaphorin3A抗骨质疏松作用靶点的研究进展

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中国全科医学 2015年第17期18卷 2105-2106,2110页

作者：郭蓓李法琦重庆医科大学附属第一医院老年病科重庆市400016

骨重塑是一个动态、持续的过程,包括两个阶段:破骨细胞作用下骨的吸收、迁移和成骨细胞作用下新骨的合成,一旦此平衡失调,就会导致严重的骨代谢疾病。目前临床中已广泛应用的抗骨质疏松药物存在一个共性问题,即抑制骨吸收的同时也抑制... 详细信息

骨重塑是一个动态、持续的过程,包括两个阶段:破骨细胞作用下骨的吸收、迁移和成骨细胞作用下新骨的合成,一旦此平衡失调,就会导致严重的骨代谢疾病。目前临床中已广泛应用的抗骨质疏松药物存在一个共性问题,即抑制骨吸收的同时也抑制了新骨的形成。而Semaphorin3A作为成骨细胞产生的一种分泌型蛋白,抑制骨吸收的同时促进了新骨的再生,在骨质疏松的治疗方面具有双向调节作用,为骨质疏松的治疗提供了新视野。本文就Semaphorin3A在抗骨质疏松作用靶点上的相关分子机制研究进展作一综述。

关键词：信号素3A 骨质疏松骨重塑破骨细胞成骨细胞