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A novel seed cone of Pinus from the Miocene of coastal Southeast China indicates kinship with Southeast Asian pines

Plant Diversity 2023年第6期45卷 732-747页

作者： Xiang-Chuan li Yi Hu Xiang Zhang liang Xiao li-na liang Rui-Zhi Zhang Lei Qiao School of Earth Sciences and Resources Chang'an UniversityXi'an 710054China State Key Laboratory of Palaeobiology and Stratigraphy Nanjing Institute of Geology and PalaeontologyChinese Academy of SciencesNanjing 210008China Shaanxi Key Laboratory of Early life and Environments Northwest UniversityXi'an 710069China Exploration Company Shaanxi Yanchang Petroleum(Group)Co.LTD.Yan'an 716099China Beijing Youth Education SciTech Co. LTD.Beijing 100089China

Pinus is an economically and ecologically important genus whose members are dominant components globally in low-latitude mountainous and mid-latitude temperate *** species richness is currently concentrated in subtropical mid-low latitudes of the Northern Hemisphere,differing from the latitudinal diversity gradient mostly recognized in woody *** the present pattern was developing in Earth's past is still poorly studied,particularly in eastern ***,a new fossil species,Pinus shengxianica *** described based on a fossil seed cone from the Late Miocene Shengxian Formation in Zhejiang,southeast China.A co-occurring cone is recognized as a known fossil species,Pinus speciosa *** comparison of extant and fossil members of Pinus suggests *** shares a striking cone similarity to Pinus merkusii and Pinus latteri(subsection Pinus)from tropical Southeast Asia in having annular bulges around the umbo on the *** morphological resemblance indicates these two extant low-latitude pines probably possess a close affinity with the present newly-discovered *** and originated from East Asian mid-low latitude ancestors during this generic re-diversification in the *** scenario is consistent with the evolutionary trajectory reflected by the pine fossil history and molecular data,marking the Miocene as a key period for the origin and evolution of most extant pines *** co-occurrences of diverse conifers and broadleaved angiosperms preferring diverse niches demonstrate Late Miocene eastern Zhejiang was one of the hot spots for coniferophyte diversity and hosted a needled-broadleaved mixed forest with complex vegetation structure and an altitudinal zonation.

关键词： Fossil cones Pinus Southeast China Miocene Phytogeography Paleoecology

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An on-line solid phase extraction-liquid chromatography tandem mass spectrometry method for the determination of perfluoroalkyl substances in the Antarctic ice core samples

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Chinese Chemical Letters 2015年第9期26卷 1073-1078页

作者： Ya-li Shi Yuan-Yuan Pan li-na liang Ya-Qi Cai State Key Laboratory of Environmental Chemistry and Ecotoxicology Research Center for Eco-Environmental ScienceChinese Academy of Sciences Thermo Fisher Scientific(China)Co. Ltd.

An on-line solid phase extraction-high performance liquid chromatography-tandem mass spectrometry method for the analysis of perfluoroalkyl substances（PFASs） in water samples was *** optimal analytical conditions were obtained through the optimization of the extraction efficiency of online solid phase extraction column,sample loading rate and loading volume,and the concentration of ammonium acetate in mobile *** the optimal condition,the analytical method displayed good linearity（r^2〉0.99） for 12 PFASs（C5-C14 perfluoroalkyl carboxylic acids and C6/C8 perfluoroalkyl sulfonic acids） over a concentration range of 0.5-100 ng/*** limits of quantitation for samples were between 0.025 ng/L and 0.5 ng/L and the relative standard deviations（RSD） of five consecutive analyses were less than 10%for 1 ng/L standard *** results were obtained using this analytical method for the analysis of perfluoroalkyl substances in Antarctic ice core *** recoveries of all perfluoroalkyl substances were in a range of 73%-117%when the samples were spiked with standards at the concentrations of 2.5 ng/L and 25 ng/L.

关键词： Perfluoroalkyl substances On-line solid phase extraction LC-MS/MS Antarctic ice cores

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Genetic, environmental and other risk factors for progression of retinitis pigmentosa

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International Journal of Ophthalmology(English edition) 2022年第5期15卷 828-837页

作者： Zi-Yang Huang li-na liang Ya-Min li Kai Xu Xiao-Yu li Eye Hospital China Academy of Chinese Medical SciencesBeijingChina

Retinitis pigmentosa(RP) is a commonly inherited disease of the retina, which is characterized by progressive loss of visual function due to specific genetic mutations. There are many risk factors that may have effect on the progression of RP, such as inheritance patterns, genotype, gender, age, smoking, physical activity, and other demographic and environmental factors. Baseline visual field conditions, changes of ellipsoid zone, photoreceptor layer thickness, and choroidal structure are reported to be the phenotype risk factors for RP progression. Moreover, aqueous flare and high-sensitivity C-reactive protein are probable inflammation biomarkers for assessing the progression of RP. Increased oxidative stress is considered to be one of the potential factors for the existence of RP. The risk factors can be combined to form a corresponding prediction model to predict disease progression. This review is to summarize the current literature that studies the genetic, environmental, phenotypic, demographic, inflammatory and other risk factors of RP progression and discuss the most reliable risk factors that could provide predictive models.

关键词： retinitis pigmentosa risk factor progression genetics phenotype inflammation prediction

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Mechanism of Mingjing Granules in Treating Wet Age-Related Macular Degeneration Based on Network Pharmacology and Experimental Verification

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World Journal of Traditional Chinese Medicine 2024年第1期10卷 22-32页

作者： Xiao-Yu li li-na liang Wei-Jun Zhang Yun Gao Qiang Chen Eye Function Laboratory Eye HospitalChina Academy of Chinese Medical SciencesBeijingChina China Evidence-Based Medicine Center for Traditional Chinese Medicine BeijingChina Center for East-West Medicine UCLA David Geffen School of MedicineLos AngelesCAUSA

Objective:To analyze the potential mechanism of Mingjing granules in the treatment of wet age-related macular degeneration(wAMD)based on the research methods of network pharmacology and molecular docking approach and to provide a new reference for the currently limited treatment of *** and Methods:We searched TCMSP,GeneCards,OMIM,PharmGkb,TTD,and DrugBank database to screen the main active ingredients of Mingjing granules and their therapeutic targets of *** network of active components and targets was constructed using Cytoscape3.6.1 software,which was also used for the topological analysis of target *** network of Protein-Protein Interactions(PPI)was mapped using the String *** also used R language to do the Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway for additional *** docking studies were finished by Chemoffice,Autodock,and ***,the efficacy of the Mingjing granules was examined in animal experiments,in which we used enzyme-linked immunosorbent assay to the contents of vascular endothelial growth factor(VEGF)and matrix metalloproteinase-9(MMP-9)levels in peripheral ***:Active compounds,including quercetin,lignocaine,and kaempferol,were *** network analysis showed that tumor necrosis factor(TNF),MMP-9,epidermal growth factor(EGF),prostaglandin-endoperoxide synthase 2(PTGS2),and caspase-3(CASP3)were related to both Mingjing granules and *** and KEGG pathway analysis showed that these targets were mainly involving lipids and atherosclerosis,TNF,and interleukin-17(IL-17)signaling *** studies suggested that quercetin and luteolin can fit in the binding pocket of four target proteins(CASP3,EGF,PTGS2,and TNF).In the vivo experiment,the Mingjing granules were found to be effective on the expression of VEGF and MMP-9 in peripheral ***:This study initially reveals the multi-constituent,multi-target,and multi-pathway mechanism of action of Ming

关键词： Experimental verification Mingjing granules molecular docking network pharmacology wet age-related macular degeneration

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LXHY Formula Inhibits Choroidal Neovascularization Development via Inhibiting the Recruitment and Adhesion of BMCs to the Retina

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World Journal of Traditional Chinese Medicine 2018年第2期4卷 62-68页

作者： li-na liang You-Zhi Tang Wen-Jie Zhan Jiao li Qun-Ying Ma Jie liang Department of Ophthalmology Research Laboratory Eye HospitalChina Academy of Chinese Medical Sciences

Objective:The objective of this study is to investigate the inhibitory effect of LXHY,a Chinese medicine compound formula,on choroidal neovascularization(CNV) and to find the possible working ***:CNV was induced in C57 BL/6 mice by krypton laser and bone marrow-derived cells(BMCs) isolated from enhanced green fluorescent protein(EGFP) transgenic mice were injected through tail vein 0.5–1 h after the laser *** BMC-treated mice were randomly divided into two groups gavaged with either distilled water(DW group) or LXHY formula solution from day 1 after laser *** days 7,14,and 28 after treatment,histopathologic examination,fundus fluorescein angiography,and choroidal flatmount assay were performed to measure the CNV severity and BMC ***4 levels in peripheral blood were measured by enzyme-linked immunosorbent *** cell-derived factor-1α(SDF?1α),vascular cell adhesion molecule-1(VCAM-1),and intercellular adhesion molecule-1(ICAM-1) were detected by immunofluorescent ***:On days 7 and 14 after treatment,CNV lesions in the LXHY-treated mice showed less recruitment of BMCs and were smaller in size compared to DW-treated *** examination also confirmed less severe CNV lesions in the LXHY ***4 levels in peripheral blood in the LXHY group were less than that of DW group on days 7 and ***,the expression levels of SDF-1α,ICAM?1,and VCAM?1 at the lesion sites in the LXHY group were lower compared with the DW ***:This experiment indicated that LXHY formula could inhibit CNV formation and development,probably by inhibiting the recruitment and attachment of BMCs into CNV area.

关键词： Age-related macular degeneration bone marrow-derived cells choroidal neovascularization LXHY formula

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Determination of common anions in oxalate by ion chromatography coupled with UV photolysis pretreatment

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Chinese Chemical Letters 2012年第11期23卷 1263-1266页

作者： Sheng lin Cao Ming li Ye Wei De Lv Guang Wen Pan Ting Ting Zhang Zhong Yang Hu li na liang Yan Zhu Department of Chemistry Zhejiang UniversityHangzhou 310028China Shanghai Lab Thermo Fisher Scientific Inc.Shanghai 201203China Hangzhou Vocational and Technical Institute Hangzhou 310018China

A new and simple method was developed to determine anions in oxalate of analytical reagent grade. After UV photolysis with optimal 1% H2O2 in 10,000 mg/L oxalate in the fabricated photoreactor, sample was directly injected into IC system. Satisfactory linearity, detections limits, good repeatability and spiked recovery were obtained. The method was successfully applied to determine anions in two commercial oxalate samples.

关键词： Ion chromatography UV photolysis Oxalate Common anions

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International Clinical Practice Guideline of Chinese Medicine Retinal Vein Occlusion

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World Journal of Traditional Chinese Medicine 2022年第3期8卷 453-461页

作者： li-Ke Xie Xiao-Feng Hao Qing-Hua Peng li-na liang Jing liu Hong-Sheng Bi Yan-Nian Hui Eye Hospital of China Academy of Chinese Medical Sciences

Foreword Please note that some of the contents of this document may involve patents, and the issuing agency of this document is not responsible for identifying *** main drafting committees of this guideline: Eye Hospital of China Academy of Chinese Medical Sciences, Hunan University of Traditional Chinese Medicine, Wangjing Hospital of China Academy of Chinese Medical Sciences, Eye Hospital of Shandong University of Traditional Chinese Medicine, Xijing Hospital of Air Force Military Medical University.

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International Clinical Practice Guideline of Chinese Medicine-Age-related Macular Degeneration

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World Journal of Traditional Chinese Medicine 2022年第4期8卷 556-564页

作者： Xing-Wei Wu li-Ke Xie You-Zhi Tang li-na liang Xiao-Feng Hao li-Xin Qiu Xin-Quan liu Wen-Xin Ma First People’s Hospital Affiliated to Shanghai Jiaotong University Eye Hospital of China Academy of Chinese Medical Sciences

Foreword Patent issues may be existed in this document, and the Word Federation of Chinese Medicine Societies declared that they are not to *** main drafting Committee of this docement:First People’s Hospital Affiliated to Shanghai Jiaotong University, Eye hospital,China academy of Chinese Medical Sciences, Beijing Tongren Hospital Affiliated to Capital Medical University, Longhua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, national Eye Institute/national Institute of Health.

关键词： Medicine Patent draft

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Inhibitory gamma-aminobutyric acidergic neurons in the anterior cingulate cortex participate in the comorbidity of pain and emotion

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Neural Regeneration Research 2025年第10期20卷 2838-2854页

作者： Lu Guan Mengting Qiu na li Zhengxiang Zhou Ru Ye liyan Zhong Yashuang Xu Junhui Ren Yi liang Xiaomei Shao Jianqiao Fang Junfan Fang Junying Du Department of Neurobiology and Acupuncture Research Third School of Clinical MedicineZhejiang Chinese Medical UniversityHangzhouZhejiang ProvinceChina Key Laboratory for Research of Acupuncture Treatment and Transformation of Emotional Diseases Third School of Clinical MedicineZhejiang Chinese Medical UniversityHangzhouZhejiang ProvinceChina Fuchun Community Health Service Center of Fuyang District HangzhouZhejiang ProvinceChina

Pain is often comorbid with emotional disorders such as anxiety and *** of the anterior cingulate cortex has been implicated in pain and pain-related negative emotions that arise from impairments in inhibitory gamma-aminobutyric acid *** review primarily aims to outline the main circuitry(including the input and output connectivity)of the anterior cingulate cortex and classification and functions of different gamma-aminobutyric acidergic neurons;it also describes the neurotransmitters/neuromodulators affecting these neurons,their intercommunication with other neurons,and their importance in mental comorbidities associated with chronic pain *** understanding on their role in pain-related mental comorbidities may facilitate the development of more effective treatments for these ***,the mechanisms that regulate gamma-aminobutyric acidergic systems remain *** is also unclear as to whether the mechanisms are presynaptic or *** exploration of the complexities of this system may reveal new pathways for research and drug development.

关键词： anterior cingulate cortex anxiety chronic pain circuit communication comorbidity depression gamma-aminobutyric acidergic neurons parvalbumin neurons synaptic transmission

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Magneto‑Dielectric Synergy and Multiscale Hierarchical Structure Design Enable Flexible Multipurpose Microwave Absorption and Infrared Stealth Compatibility

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nano-Micro Letters 2025年第2期17卷 401-416页

作者： Chen li Leilei liang Baoshan Zhang Yi Yang Guangbin Ji School of Electronic Science and Engineering Nanjing UniversityNanjing 210093People’s Republic of China College of Materials Science and Technology Nanjing University of Aeronautics and AstronauticsNanjing 210016People’s Republic of China

Developing advanced stealth devices to cope with radar-infrared(IR)fusion detection and diverse application scenarios is increasingly demanded,which faces significant challenges due to conflicting microwave and IR cloaking mechanisms and functional integration ***,we propose a multiscale hierarchical structure design,integrating wrinkled MXene IR shielding layer and flexible Fe_(3)O_(4)@C/PDMS microwave absorption *** top wrinkled MXene layer induces the intensive diffuse reflection effect,shielding IR radiation signals while allowing microwave to pass ***,the permeable microwaves are assimilated into the bottom Fe_(3)O_(4)@C/PDMS layer via strong magneto-electric *** theoretical and experimental optimization,the assembled stealth devices realize a near-perfect stealth capability in both X-band(8–12 GHz)and long-wave infrared(8–14μm)wavelength ***,it delivers a radar cross-section reduction of−20 dB m^(2),a large apparent temperature modulation range(ΔT=70℃),and a low average IR emissivity of ***,the optimal device demonstrates exceptional curved surface conformability,self-cleaning capability(contact angle≈129°),and abrasion resistance(recovery time≈5 s).This design strategy promotes the development of multispectral stealth technology and reinforces its applicability and durability in complex and hostile environments.

关键词： Microwave absorption Radar-infrared compatible stealth Wrinkled MXene Magneto-dielectric synergy Multifunction

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