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Positive and negative shear lag behaviors of composite twin-girder decks with varying cross-section

Science China(Technological Sciences) 2017年第1期60卷 116-132页

作者： ZHU li nie JianGuo JI WenYu Key Laboratory of Civil Engineering Safety and Durability of China Education Ministry Department of Civil Engineering Tsinghua University Beijing 100084 China

In steel-concrete composite twin-girder decks, wide concrete slab would undergo significant shear lag warping effect, including positive and negative. Some researchers have investigated the positive shear lag of composite decks by means of one-dimensional line model, while the studies on the negative shear lag have not yet been reported until now. In this study, a new one-dimensional analytical model of composite twin-girder decks is first proposed based on the model proposed by Dezi et al. Besides slab shear lag effect and partial connection at slab-girder interface which have been included in the model of Dezi et al., the particularity of the proposed model relies on its ability to account for variation characteristic of cross-section. Verification of the analytical model is later conducted through comparison of results from the analytical analysis and elaborate FE analysis for a simply supported composite deck with increasing depth and a two-span continuous one with decreasing depth. Finally, three kinds of structural forms of composite twin-girder decks, including cantilever, simply supported and continuous decks, are selected to carry out the analysis of positive and negative shear lag behaviors by means of the analytical model. The influences of cross-sectional variation characteristic and load type on positive and negative shear lag behaviors are mainly investigated. Additionally, a new definition on effective width for considering simultaneously positive and negative shear lag behaviors is proposed. The results from the proposed analytical model and EC4 specification are compared to provide suggestions for designers and checkers. In this study, the proposed analytical model can provide a powerful numerical tool for researchers to conduct the further investigation, and the analysis on shear lag and effective width can assist in design analysis of composite twin-girder decks.

关键词： steel-concrete composite twin-girder deck analytical model variation of cross-section positive and negative shear lagbehavior effective width

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MOSPD2 is a receptor mediating the LEAP-2 effect on monocytes/macrophages in a teleost,Boleophthalmus pectinirostris

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Zoological Research 2020年第6期41卷 644-655页

作者： Chang-Hong li Jie Chen li nie Jiong Chen State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products Ningbo UniversityNingboZhejiang 315211China Laboratory of Biochemistry and Molecular Biology School of Marine SciencesMeishan CampusNingbo UniversityNingboZhejiang 315832China Key Laboratory of Applied Marine Biotechnology of Ministry of Education Meishan CampusNingbo UniversityNingboZhejiang 315832China College of Ecology Lishui UniversityLishuiZhejiang 323000China

liver-expressed antimicrobial peptide 2(LEAP-2)is a cationic peptide that plays an important role in a host’s innate immune *** previously demonstrated that mudskipper(Boleophthalmus pectinirostris)LEAP-2(BpLEAP-2)induces chemotaxis and activation of monocytes/macrophages(MO/MΦ).However,the molecular mechanism by which BpLEAP-2 regulates MO/MΦ remains *** this study,we used yeast twohybrid cDNA library screening to identify mudskipper protein(s)that interacted with BpLEAP-2,and characterized a sequence encoding motile sperm domain-containing protein 2(BpMOSPD2).The interaction between BpLEAP-2 and BpMOSPD2 was subsequently confirmed by co-immunoprecipitation(Co-IP).Sequence analyses revealed that the predicted BpMOSPD2 contained an N-terminal extracellular portion composed of a CRAL-TRIO domain and a motile sperm domain,a C-terminal transmembrane domain,and a short cytoplasmic *** tree analysis indicated that BpMOSPD2 grouped tightly with fish MOSPD2 homologs and was most closely related to that of the Nile tilapia(Oreochromis niloticus).The recombinant BpMOSPD2 was produced by prokaryotic expression and the corresponding antibody was prepared for protein concentration *** interference was used to knockdown BpMOSPD2 expression in the mudskipper MO/MΦ,and the knockdown efficiency was confirmed by quantitative real-time polymerase chain reaction(qRT-PCR)and western *** of BpMOSPD2 significantly inhibited BpLEAP-2-induced chemotaxis of mudskipper MO/MΦand BpLEAP-2-induced bacterial killing ***,knockdown of BpMOSPD2 inhibited the effect of BpLEAP-2 on mRNA expression levels of BpIL-10,BpTNFα,BpIL-1β,and BpTGFβ in MO/MΦ.In general,BpMOSPD2 directly interacted with BpLEAP-2,and mediated the effects of BpLEAP-2 on chemotaxis and activation of mudskipper MO/MΦ.This is the first identification of MOSPD2 as a receptor for LEAP-2.

关键词： LEAP-2 Monocyte/macrophage MOSPD2 RNA interference Yeast two-hybrid

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Current understanding on the roles of gut microbiota in fish disease and immunity

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Zoological Research 2019年第2期40卷 70-76页

作者： Jin-Bo Xiong li nie Jiong Chen Laboratory of Biochemistry and Molecular Biology School of Marine Sciences Ningbo University Key Laboratory of Applied Marine Biotechnology of Ministry of Education Ningbo University

Intensive aquaculture has increased the severity and frequency of fish *** the functional importance of gut microbiota in various facets of host physiology,modulation of this microbiota is a feasible strategy to mitigate emerging diseases in *** achieve this,a fundamental understanding of the interplay among fish health,microbiota, and invading pathogens is *** commentary focuses on current knowledge regarding the associations between fish diseases,dysbiosis of gut microbiota,and immune responses. Furthermore,updated research on fish disease from an ecological perspective is discussed,including colonization resistance imposed by commensals and strategies used by pathogens to overcome *** also propose several directions for future research,such as exploration of the causal links between fish diseases and specific taxa,and identification of universal gut microbial biomarkers for rapid disease diagnosis.

关键词： microbiota fish disease immunity

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Studies on Non-potentiometric Piezoelectric Sensor System for Determination of Vitamin B_1

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Chemical Research in Chinese Universities 2003年第3期19卷 280-285页

作者： YUAN Jin bin nie li hua YAO Shou zhuo State Key Laboratory of Chemo/Biosensing&Chemometrics College of Chemistry and Chemical EngineeringHunan UniversityChangsha 410082P.R.China

A piezoelectric sensor responsive to vitamin B 1 was fabricated based on the vitamin B 1 tetraphenylborate ion pair. The general performance characteristics of the sensor are presented here. The proposed sensor showed a wide working pH range, a good sensitivity and selectivity. The response range is between 1 0×10 -7 -4 9×10 -5 mol/L with a detection limit of 8×10 -8 mol/L at pH 4 0. The selectivity should be attributed to the preferential adsorption of the component ion on the membrane/solution interface. The adsorption behavior of vitamin B 1 on the crystal surface was investigated with a quartz crystal impedance(QCI) system.

关键词： Piezoelectric sensor Vitamin B_(1) Ion-pair complex

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Development of Near Infrared Spectroscopy for Rapid Quality Assessment of Red Ginseng

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Chemical Research in Chinese Universities 2009年第5期25卷 633-637页

作者： nie li-xing WANG Gang-li liN Rui-chao National Institute for the Control of Pharmaceutical and Biological Products Beijing 100050 P. R. China

Near infrared spectroscopy（NIRS） was developed as a rapid analysis method for the qualitative and quantitative assessment of the quality of red ginseng. Discriminant analysis（DA） based on principal component analysis and Mahalanobis distance was used to distinguish red ginseng from counterfeits non-destructively. The result shows that the proposed method could distinguish red ginseng from counterfeits correctly and no misclassified sample was found in both training and test sets. The partial least squares（PLS） algorithm was used to predict the sum of ginsenosides Re and Rgl and the content of ginsenoside Rb1. Two calibration models were developed to correlate NIR spectra with the reference values determined by HPLC method. The correlation coefficient（R）, the root mean square error of calibration（RMSEC） and the root mean square error of prediction（RMSEP） were as follows： R=0.9827, RMSEC=0.0163%, RMSEP=0.0250% for the sum of ginsenosides Re and Rgl; R=0.9869, RMSEC=0.0156%, RMSEP=0.0256% for content of ginsenoside Rb1. The overall results demonstrate that NIRS coupled with chemometrics could be successfully applied as a rapid, precise and cost-effective method not only to identify the red ginseng from counterfeits but also to determine simultaneously some chemical compositions in red ginseng.

关键词： Near infrared soectroscopy Red ginseng Discriminant analysis Partial least squares

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Effects of Headgroups and Serum on Gene Transfection of Alkaline Amino Acid Based Cationic lipids

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Chemical Research in Chinese Universities 2009年第1期25卷 69-71页

作者： li li YANG Yang nie Yu HE Bin GU Zhong-wei National Engineering Research Center for Biomaterials Sichuan University Chengdu 610064 P. R. China

Three cationic lipids with lysylated（1）, histidylated（2）, and arginylated（3） headgroups and cholesterol hydrophobic moiety were synthesized. The average sizes of liposomes and lipoplexes were around 100 and 160 nm, respectively. The gene transfection efficiency of the three lipoplexes loaded with pGL3 or pORF-LacZ was compared on 293T cells in the presence or the absence of serum. The transfection efficiency of the three lipoplexes in a serum-free medium was 2 to 3-fold higher than that of dioleoyl-trimethylammonium propane（DOTAP）. In the presence of serum, however, most of the lipoplexes showed lower transfection activities; only lipoplex 3 retained its high transfection efficiency.

关键词： Cationic lipids Alkaline amino acids Serum Gene delivery Transfection efficiency

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Probing the weak interactions between amino acids and carbon monoxide

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Chinese Chemical Letters 2008年第1期19卷 119-122页

作者： Ying Wu lin Chang Ming nie li Fu liao College of Chemistry and Chemical Engineering University of South China Hengyang 421001 China

The relatively weak interactions between some non-charged amino acids with CO were investigated theoretically by using density functional theory （DFT） method. The stretching frequency of CO correlates well with its bond length followed the Badger＇s rule, and the correlation between binding energy and Mulliken charge partition of CO was also investigated for the first time. 2007 Ying Wu lin. Published by Elsevier B.V. on behalf of Chinese Chemical Society. All rights reserved.

关键词： Amino acid CO Interaction Orientation Binding energy

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Effect of Sc on Al_(3)Fe phase and mechanical properties of as-cast AA5052 aluminum alloy

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China Foundry 2024年第3期21卷 257-264页

作者： Yang li Qing Yu Feng-feng Chen Jia-wen He Hong-mei Yang Meng-nie li Faculty of Materials Science and Engineering Kunming University of Science and TechnologyKunming 650093China

The AA5052 aluminum alloy is widely used in automobile and aerospace manufacturing,and with the development of light-weight alloys,it is required that these materials exhibit better mechanical *** studies have demonstrated that the addition of Sc to aluminum alloys can improve both the microstructure and properties of the *** this study,the effect of Sc on the Fe-rich phase and properties of the AA5052 aluminum alloy was studied by adding 0%,0.05%,0.2%,and 0.3%*** results show that with the increase of Sc,the coarse needle-like Fe-rich phase gradually transforms into Chinese-script and then nearly spherical particles,reduce the size of Fe-rich phase,and refine the grain with increase of high angle grain boundaries(HAGBs).These microstructure changes enhance the strength of the AA5052 alloy through Sc *** ductility of the alloy is obviously improved because the addition of a lower amount of Sc changes the morphology of Fe-rich phase from needle-like into a Chinese-script,and it is subsequently reduced as a result of significant increase in HAGBs with increasing Sc content.

关键词： AA5052 aluminum alloy Al3Fe phase mechanical properties grain boundary

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RIG-I： a multifunctional protein beyond a pattern recognition receptor

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Protein & Cell 2018年第3期9卷 246-253页

作者： Xiao-xiao Xu Han Wan li nie Tong Shao li-xin Xiang Jian-zhong Shao Key Laboratory for Cell and Gene Engineering of Zhejiang Province College of Life Sciences Zhejiang University Hangzhou 310058 China Laboratory for Marine Biology and Biotechnology Qingdao National Laboratory for Marine Science and Technology Qingdao 266235 China

It was widely known that retinoic acid inducible gene I （RIG-I） functions as a cytosolic pattern recognition receptor that initiates innate antiviral immunity by detecting exogenous viral RNAs. However, recent stud- ies showed that RIG-I participates in other various cellular activities by sensing endogenous RNAs under different circumstances. For example, RIG-I facilitates the therapy resistance and expansion of breast cancer cells and promotes T cell-independent B cell activation through interferon signaling activation by recognizing non-coding RNAs and endogenous retroviruses in certain situations. While in hepatocellular carcinoma and acute myeloid leukemia, RIG-I acts as a tumor suppressor through either augmenting STAT1 activation by competitively binding STAT1 against its negative regulator SliP1 or inhibiting AKT-mTOR signaling pathway by directly interacting with Src respectively. These new findings suggest that RIG-I plays more diverse roles in various cellular life activities, such as cell proliferation and differentiation, than previously known. Taken together, the function of RIG-I exceeds far beyond that of a pattern recognition receptor.

关键词： RIG-I viral RNA endogenous RNA immunity cancer

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Progress in research on the application of nursing models for breast cancer patients during the perioperative period

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Frontiers of Nursing 2018年第2期5卷 83-90页

作者： li-Ting nie Qiao-Yuan Yan School of Nursing Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei 430030China Union Hospital Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei 430022China

Nursing models at home and abroad for breast cancer patients during the perioperative period were screened, including eight types of models： the nursing model guided by self-care theory, the plan-do-check-act cycle combined with the four-in-one model, the peer support nursing model, the nursing model guided by transcultural theory, the multidisciplinary cooperative nursing model, the knowledge-attitude-practice nursing model, the safe nursing management model, and the case nursing model. These models were analyzed and described with the aim of providing a reference for the clinical breast surgery nursing staff in China and for promoting the development of nursing in China for breast cancer the perioperative period.

关键词： Nursing models breast cancer perioperative period literature review

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