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Mussel-inspired multifunctional coating for bacterial infection prevention and osteogenic induction

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Journal of Materials Science & Technology 2021年第9期68卷 160-171页

作者： mingjun li Christoph Schlaich Jianguang Zhang Ievgen S.Donskyi Karin Schwibbert Frank Schreiber Yi Xi Jorg Radnik Tanja Schwerdtle Rainer Haag Center for Health Science and Engineering Tianjin Key Laboratory of Materials Laminating Fabrication and Interface Control TechnologySchool of Materials Science and EngineeringHebei University of TechnologyTianjin 300130China Institut fur Chemie und Biochemie Freie Universitat BerlinBerlin 14195Germany Division Biodeterioration and Reference Organisms Federal Institute for Materials Research and Testing(BAM)Berlin 12205Germany Division of Surface Analysis and Interfacial Chemistry Federal Institute for Materials Research and Testing(BAM)Berlin 12205Germany Institute of Nutritional Science Department of Food ChemistryUniversity of PotsdamNuthetal D-14558Germany

Bacterial infection and osteogenic integration are the two main problems that cause severe complications after surgeries. In this study, the antibacterial and osteogenic properties were simultaneously introduced in biomaterials, where copper nanoparticles(Cu NPs) were generated by in situ reductions of Cu ions into a mussel-inspired hyperbranched polyglycerol(MI-h PG) coating via a simple dip-coating *** hyperbranched polyglycerol with 10 % catechol groups’ modification presents excellent antifouling property, which could effectively reduce bacteria adhesion on the surface. In this work, polycaprolactone(PCL) electrospun fiber membrane was selected as the substrate, which is commonly used in biomedical implants in bone regeneration and cardiovascular stents because of its good biocompatibility and easy post-modification. The as-fabricated Cu NPs-incorporated PCL membrane [PCL-(MI-h PG)-Cu NPs]was confirmed with effective antibacterial performance via in vitro antibacterial tests against Staphylococcus aureus(S. aureus), Escherichia coli(E. coli), and multi-resistant E. coli. In addition, the in vitro results demonstrated that osteogenic property of PCL-(MI-h PG)-Cu NPs was realized by upregulating the osteoblast-related gene expressions and protein activity. This study shows that antibacterial and osteogenic properties can be balanced in a surface coating by introducing Cu NPs.

关键词： Mussel-inspired coating CuNPs Multi-resistant bacteria Antibacterial Antifouling Osteogenesis

Applications of cold atmospheric plasmas(CAPs)in agriculture:a brief review and the novel development of a radio-frequency CAP jet generator for plant mutation

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Plasma Science and Technology 2022年第9期24卷 1-14页

作者： Heping li Jing li Xiting ZHAO liyan WANG Chuan FANG Nan SU Chong ZHANG Yan XU Meng LV mingjun li Xinhui XING Department of Engineering Physics Tsinghua UniversityBeijing 100084People’s Republic of China College of life Sciences Henan Normal UniversityXinxiang 453007People’s Republic of China Biobreeding Research Center Wuxi Research Institute of Applied TechnologiesTsinghua UniversityWuxi 214072People’s Republic of China MOE Key Laboratory of Industrial Biocatalysis Department of Chemical EngineeringTsinghua UniversityBeijing 100084People’s Republic of China Center for Systems and Synthetic Biology Tsinghua UniversityBeijing 100084People’s Republic of China Institute of Biopharmaceutical and Health Engineering Tsinghua Shenzhen International Graduate SchoolShenzhen 518055People’s Republic of China

Cold atmospheric plasmas(CAPs)have shown great applicability in *** kinds of CAP sources have been studied in agricultural applications to promote plant growth and cure plant *** briefly review the state-of-the-art stimulating effects of atmospheric-pressure dielectricbarrier-discharge(AP-DBD)plasmas,after the direct or indirect treatment of plants for growth promotion and disease *** then discuss the special demands on the characteristics of the CAP sources for their applications in plant mutation *** atmospheric and room temperature plasma(ARTP)jet generator with a large plasma irradiation area,a high enough concentration of chemically reactive species and a low gas temperature is designed for direct plant *** measurements of the electrical,thermal and optical features of the ARTP generator are ***,an ARTP-P(ARTP for plant mutagenesis)mutation breeding machine is developed,and a typical case of plant mutation breeding by the ARTP-P mutation machine is presented using Coreopsis tinctoria *** and agricultural experiments show that the newly-developed ARTP-P mutation breeding machine with a large irradiation area can generate uniform CAP jets with high concentrations of chemically reactive species and mild gas temperatures,and have signiflcant mutagenesis effects on the Coreopsis tinctoria *** ARTP-P mutation breeding machine may provide a platform for systematic studies on mutation mechanisms and results for various plant seeds under different operating conditions in future research.

关键词： cold atmospheric plasma radio-frequency glow discharge mutation plant breeding ARTP agricultural application

小细胞肺癌肌球蛋白重链9表达下调参与细胞机械感知功能缺失

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中华实验外科杂志 2024年第6期41卷 1241-1244页

作者：李明君杨宁宁罗明董卫萍王文刚郑州大学第一附属医院肿瘤科郑州450052 郑州大学第一附属医院急诊科郑州450052 武汉大学中南医院骨科武汉430062 郑州大学第一附属医院乳腺外科郑州450052 郑州大学第一附属医院骨科郑州450052

目的探究小细胞肺癌的机械感知功能及其关键蛋白肌球蛋白重链9(MYH9)在细胞失巢凋亡中的作用。方法采用3 kPa和12 kPa基质刚度水凝胶,比较小细胞肺癌细胞的Paxillin面积和细胞极化。组织和细胞系水平从11个重要机械感知蛋白中筛选关键蛋... 详细信息

目的探究小细胞肺癌的机械感知功能及其关键蛋白肌球蛋白重链9(MYH9)在细胞失巢凋亡中的作用。方法采用3 kPa和12 kPa基质刚度水凝胶,比较小细胞肺癌细胞的Paxillin面积和细胞极化。组织和细胞系水平从11个重要机械感知蛋白中筛选关键蛋白MYH9。通过慢病毒敲减和过表达MYH9表达水平,明确MYH9对细胞机械感知功能的作用,采用流式细胞膜联蛋白V(Annexin V)/碘化丙锭(PI)双染法测定MYH9对细胞失巢凋亡能力的影响。两组连续变量采用独立样本的t检验,3组组间比较采用One-Way ANOVA分析。结果人支气管上皮细胞的软基质组黏着斑面积[(0.79±0.17)μm^(2)比(1.68±0.30)μm^(2),t=8.26,P0.05]和细胞形态比值(1.26±0.20比1.36±0.22,t=1.06,P>0.05)较硬基质组差异无统计学意义。筛选基因表达综合数据库(GEO)中小细胞肺癌组织的转录组测序数据,小细胞肺癌细胞组MYH9蛋白表达(0.12±0.02比0.30±0.02,t=11.44,P<0.01)低于对照组。过表达小细胞肺癌细胞中MYH9后,软基质组黏着斑面积[(0.69±0.21)μm^(2)比(1.12±0.43)μm^(2),t=2.85,P<0.05]和细胞形态比值(1.37±0.18比1.70±0.34,t=2.72,P<0.05)低于硬基质组,漂浮培养下的早期凋亡细胞比例[(12.81±1.92)%比(4.81±1.30)%,t=7.69,P<0.01]高于硬基质组。结论小细胞肺癌细胞的机械感知功能缺失,其关键功能蛋白MYH9显著下调。过表达MYH9在恢复小细胞肺癌细胞机械感知功能的同时,削弱其抗脱落凋亡能力。

关键词：小细胞肺癌机械感知脱落凋亡

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安徽某铁矿高压辊磨系统优化试验

现代矿业 2024年第3期40卷 117-121页

作者：王荣林李明军杨松付王欢常鲁平王帅安徽马钢矿业资源集团姑山矿业有限公司中钢集团马鞍山矿山研究总院股份有限公司

安徽某选厂为提高处理量,以全铁品位35.17%的中碎产品为试样,进行了2种不同方案的高压辊磨系统优化试验研究。中碎产品全部预先筛分—筛上干选—辊磨试验不同筛孔尺寸预先筛分试验结果表明:给矿中细粒级越少,越有利于新生成-5 mm粒级产... 详细信息

安徽某选厂为提高处理量,以全铁品位35.17%的中碎产品为试样,进行了2种不同方案的高压辊磨系统优化试验研究。中碎产品全部预先筛分—筛上干选—辊磨试验不同筛孔尺寸预先筛分试验结果表明:给矿中细粒级越少,越有利于新生成-5 mm粒级产品,预先筛分筛孔尺寸为5 mm时,新生成的-5 mm粒级含量最多,达57.99%。中碎产品部分预先筛分—筛上产品干选合并未筛分给矿高压辊磨试验结果表明:随着中碎产品分矿比例的降低,辊压给矿中-5 mm粒级含量降低,新生成-5 mm粒级含量增加;当分矿比为3∶1时,新生成的-5 mm粒级含量最高,为45.36%。试验结果对比表明,采用全部预先筛分—筛上干选—辊磨试验方案指标最好,较中碎产品直接进行高压辊磨,新生成-5 mm粒级含量提高25.47个百分点。

关键词：高压辊磨预先筛分干选磁铁矿

Spectroscopic and temperature sensing properties of Sm3+ in self-activated CsLu（WO4）2 phosphors

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Journal of Rare Earths 2024年第11期42卷 2033-2042页

作者： mingjun Song Jing Wang Zhi Xie li liu Wang Zhao Weiwei Zhou School of Chemistry Chemical and Environmental EngineeringWeifang University College of Mechanical and Electrical Engineering Fujian Agriculture and Forestry University School of Chemistry and Materials Engineering Huainan Normal University

In this research,a series of Sm3+doped CsLu（WO4）2phosphors was prepared via high temperature solid phase technique to design new red phosphors and optical thermometric *** structures,morphology,band gap and luminescence properties were characterized by X-ray diffraction,scanning electron microscopy,diffuse reflection and luminescence spectra,*** UV excitation,CsLu（WO4）2gives rise to a blue broad emission band between 350 and 700 nm,which stems from the3T1u→1A1gtransition of *** Sm3+is introduced into CsLu（WO4）2,energy transfer between WO66-groups and Sm3+ions takes place in CsLu（WO4）2:Sm3+phosphors,and color-tunable luminescence from blue to red is realized by controlling the Sm3+doping *** energy transfer efficiency between WO66-groups and Sm3+ons was analyzed,and the energy transfer mechanism was determined to be dipole-dipole *** to the temperature-dependent luminescence spectra,WO66-groups and Sm3+ions exhibit large discrepancy in thermal quenching rates,and thus the temperature sensing properties of CsLu（WO4）2:Sm3+in the temperature range of 283-403 K were *** on the framework of fluorescence intensity ratio theory,the basic optical thermometry parameters including absolute and relative sensitivity of CsLu（WO4）2:Sm3+we re calculated and the results show that it has great potential for application in optical thermometry.

关键词： Energy transfer Fluorescence intensity ratio Optical thermometry Phosphors Rare earths

关节镜下全缝线锚钉治疗肩袖损伤的短期临床疗效

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中国运动医学杂志 2024年第1期43卷 3-10页

作者：鲍涛胡洋洋巩绪勇王硕果王亮杨健费文勇卢耀甲杨宇夏刘殿伟党孟博李明俊扬州大学临床医学院扬州225001 江苏省苏北人民医院运动医学科日间手术中心扬州225001 大连医科大学大连116044

目的:采用前瞻性、单队列临床试验评估关节镜下应用全缝线锚钉修复肩袖损伤的短期临床疗效。方法:以江苏省苏北人民医院运动医学科、日间手术中心自2022年7月至2022年9月收治的25例肩袖损伤(1.5 cm0.05)。结论:关节镜下应用全缝线锚钉... 详细信息

目的:采用前瞻性、单队列临床试验评估关节镜下应用全缝线锚钉修复肩袖损伤的短期临床疗效。方法:以江苏省苏北人民医院运动医学科、日间手术中心自2022年7月至2022年9月收治的25例肩袖损伤(1.5 cm0.05)。结论:关节镜下应用全缝线锚钉进行肩袖修补术具有可行性和安全性,短期临床疗效较好。

关键词：全缝线锚钉肩关节肩袖修补肩袖损伤锚周囊肿

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水冷永磁防爆电机十字形肋片的优化设计

黑龙江科技大学学报 2024年第1期34卷 106-111页

作者：吕品王浩铭李明军程鲁帅王凯旋翟悦琳黑龙江科技大学电气与控制工程学院哈尔滨150022

为了研究不同水冷结构对水冷永磁电机散热性能的影响,以一台水冷永磁电机为研究对象,优化十字形肋片结构对轴向“Z”字形水路,通过分析加入水道中的十字形肋片的结构参数,确定横肋的高度和数量两个影响参数,采用Workbench对电机进行三... 详细信息

为了研究不同水冷结构对水冷永磁电机散热性能的影响,以一台水冷永磁电机为研究对象,优化十字形肋片结构对轴向“Z”字形水路,通过分析加入水道中的十字形肋片的结构参数,确定横肋的高度和数量两个影响参数,采用Workbench对电机进行三维建模,获得不同横肋高度和数量下的电机各部温度分布。结果表明,对比分析电机各部分温升,得到最优冷却结构的横肋数量为3时冷却效果最佳,定子、绕组、转子和永磁体的最高温度分别下降15.32、15.41、11.24和11.24℃。

关键词：永磁电机冷却水道十字形肋片

The integration of transcriptomic and transgenic analyses reveals the involvement of the SA response pathway in the defense of chrysanthemum against the necrotrophic fungus Alternaria sp.

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Horticulture Research 2020年第1期7卷 1744-1757页

作者： Xiting Zhao lingyu Song liwei Jiang Yuting Zhu Qinghui Gao Dandan Wang Jing Xie Meng Lv Ping liu mingjun li College of life Sciences Henan Normal UniversityXinxiang 453007China Engineering Technology Research Center of Nursing and Utilization of Genuine Chinese Crude Drugs in Henan Province Xinxiang 453007China College of Mathematics and Information Science Henan Normal UniversityXinxiang 453007China

Chrysanthemum morifolium cv.‘Huaihuang’has ornamental,edible,medicinal,and tea product ***,its field growth,yield,and quality are negatively affected by black spot disease caused by Alternaria sp.(Strain:HQJH10092301;GenBank accession number:KF688111).In this study,we transcriptionally and transgenically characterized a new cultivar,‘Huaiju 2#’(Henan Traditional Chinese Medicine Plant Cultivar identification number:2016002),which was bred from‘Huaihuang’and shows resistance to Alternaria ***‘Huaiju 2#’plants were inoculated with Alternaria *** three or five *** analysis showed increases in both salicylic acid(SA)and jasmonic acid(JA)in infected plants compared to the *** activity analysis also revealed a significant increase in defense enzyme activities in infected ***-Seq of plants infected for 3 or 5 days produced a total of 58.6 GB of clean *** these reads,16,550 and 13,559 differentially expressed genes(DEGs)were identified in Cm_3 dpi(sample from 3 days post-inoculation labeled as Cm_3 dpi)and Cm_5 dpi(sample from 5 days post-inoculation labeled as Cm_5 dpi),respectively,compared with their controls(Cm_0 d:a mixture samples from 0 d(before inoculation)and those treated with sterile distilled water at 3 dpi and 5 dpi).Gene annotation and cluster analysis of the DEGs revealed a variety of defense responses to Alternaria ***,which were characterized by increases in resistance(R)proteins and the reactive oxygen species(ROS),Ca2+,mitogen-activated protein kinase(MAPK),and JA signaling *** particular,SA signaling was highly responsive to Alternaria *** qPCR analysis of 12 DEG candidates supported their differential expression characterized by using the RNA-Seq *** candidate was CmNPR1(nonexpressor of pathogenesis-related gene 1),an important positive regulator of SA in systemic acquired resistance(SAR).Overexpression of CmNPR1 in‘Huaiju 2#’increased the resistance of transgen

关键词： sterile cultivar integration

Investigation of pore structure alteration and permeability enhancement of shale matrix by supercritical water treatment after hydraulic fracturing

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Petroleum 2024年第2期10卷 265-274页

作者： Yili Kang Peisong li Wangkun Cao mingjun Chen lijun You Jiang liu Zhehan Lai State Key Laboratory of Oil and Gas Reservoir Geology and Exploitation Southwest Petroleum UniversityChengdu610500China Institute of Engineering Technology Petro China Coalbed Methane Company LimitedXi'an710082China Chemical and Petroleum Engineering University of CalgaryCalgaryT2N 1N4Canada

Shale gas reservoirs are unconventional tight gas reservoirs,in which horizontal wells and hydraulic fracturing are required to achieve commercial *** fracture networks created by hydraulic fracturing can increase the drainage area extensively to enhance shale gas ***,large volumes of fracturing fluid that is difficult to flow back to the surface and remained in the shale formation,will inevitably lead to damages of the shale formations and limit the effectiveness of *** water(SCW)treatment after hydraulic fracturing is a new method to enhance shale gas recovery by using appropriate heat treatment methods to the specific formation to convert the retained fracturing fluid into a supercritical state(at temperatures in excess of 373.946°C and pressures in excess of 22.064 MPa).An experiment was conducted to simulate the reaction between shale and SCW,and the capacity of SCW treatment to enhance the permeability of the shale was evaluated by measuring the response of the shale porosity and permeability on SCW *** experimental results show that the shale porosity and permeability increase by 213.43%and 2198.37%,*** pore structure alteration and permeability enhancement of the shale matrix were determined by analyzing the changes in pore structure and mineral composition after SCW *** mechanisms that affect pore structure and mineral composition include oxidative catalysis decomposition of organic matters and reducing minerals,acid-catalyzed decomposition of carbonate minerals and feldspar minerals,hydrothermal catalysis induced fracture extension and cementation weakening induced fracture *** treatment converts harm into a benefit by reducing the intrusion of harmful substances into the shale formation,which will broaden the scope and scale of shale formation stimulation.

关键词： Shale gas Formation damage control Formation heat treatment Supercritical water