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Acta Oceanologica Sinica 2017年第2期36卷 78-87页

作者： WANG Zhen li Yang liN Xuezheng The first institute of {2. Key Laboratory of Marine Bioactive Substances State Oceanic Administration Qingdao 266061 China

The key functional genes involved in temperature adaption of the Antarctic psychrotrophic bacterium Psychrobacter sp. G. were identified by transcriptomic sequencing. We analyzed the global transcriptional profile of Psychrobacter sp. G under cold stress（0°C） and heat stress（30°C）, with the optimal growth temperature 2.°C as the control. There were large alterations of the transcriptome profile, including significant upregulation of 11 and 12.transcripts as well as significant downregulation of 47 and 42.transcripts in the cold and heat stress groups, respectively, compared to the control. The expression of various genes encoding enzymes and transcriptional regulators, including Pfp I and Tet R family transcriptional regulators under heat stress, as well as the expression of DEAD/DEAH box helicase and the Icl R family of transcriptional regulators under cold stress, were upregulated significantly. The expression of several genes, most affiliated with Ton Bdependent receptor and siderophore receptor, was downregulated significantly under both heat and cold stress. Many of the genes associated with the metabolism of fatty acid and ABC transporters were regulated differentially under different temperature stress. The results of this survey of transcriptome and temperature stress-relevant genes contribute to our understanding of the stress-resistant mechanism in Antarctic bacteria.

关键词： Psychrobacter temperature stress transcriptome Antarctic

Hydrogen Peroxide Sensor Based on Horseradish Peroxidase Combined with CaCO_3 Microspheres and Gold Nanoparticles

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Chemical Research in Chinese Universities 2011年第5期27卷 875-879页

作者： ZHENG li JIANG Feng-hua MA Guang-ran ZHUANG Qian-fen li Feng Marine Ecology Research Center First Institute of Oceanography State Oceanic Administration Qingdao 266061 P. R. China Shandong Provincial Key Laboratory of Biochemical Analysis College of Chemistry and Molecular Engineering Qingdao University of Science and Technology Qingdao 266042 t~ R. China

Direct electrochemistry and electrocatalysis of horseradish peroxidase（HRP） were achieved by entrapping the enzyme between CaCO3 microspheres and gold nanoparticles through forming sandwich configuration （CaCO3-HRP-AuNPs）. Polyanion, poly（styrene sulfonate）（PSS）, was hybrid with CaCO3 microspheres to increase the surface negative charges for binding with HRP through electrostatic interaction. After the bioconjugate CaCO3 PSS-HRP was entrapped in chitosan based sol-gel（CS-GPTMS） film, HRP was encapsulated by in situ formation of an outer layer of AuNPs through electrochemical reduction of HAuCl4. The composite film containing AuNPs, CaCO3-PSS-HRP bioconjugates and CS-GPTMS can provide favorable microenvironment for HRP to perform direct electron transfer at glassy carbon electrode（GCE）. HRP retained its bioelectrocatalytic activity and lead to sensitive and fast amperometric response for the determination of H2.2. H2.2.could be detected in a very wide linear range from 5.0×10-6 mol/L to 7.1×10-2.mol/L. The sandwich configuration of CaCO3-biomolecules-AuNPs could serve as a versatile platform for enzyme immobilization and biosensing.

关键词： Direct electrochemistry Horseradish peroxidase(HRP) CaCO3 microsphere Chitosan based sol-gel

台湾东北部海域海表温度季节与年际异常及其对历史气候重建的启示

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地球科学 2018年第3期43卷 851-861页

作者：赵松常凤鸣李铁刚庄丽华熊志方徐烨中国科学院海洋研究所海洋地质与环境重点实验室山东青岛266071 青岛海洋科学与技术国家实验室海洋地质过程与环境功能实验室山东青岛266061 中国科学院大学地球科学学院北京100049 国家海洋局第一海洋研究所海洋沉积与环境地质国家海洋局重点实验室山东青岛266061

SST(海洋表层温度,sea surface temperature)的季节与年际异常对于认识现代全球变暖、重建历史时期气候变化以及探讨气候变化机制具有重要意义,而台湾东北部海域SST季节与年际异常的研究却相对较少.为更好地理解现代全球变暖和历史气候... 详细信息

SST(海洋表层温度,sea surface temperature)的季节与年际异常对于认识现代全球变暖、重建历史时期气候变化以及探讨气候变化机制具有重要意义,而台湾东北部海域SST季节与年际异常的研究却相对较少.为更好地理解现代全球变暖和历史气候变化,利用NOAA的全球海表温度最优插值资料、Hadley中心的全球海表温度数据以及MEI逐月指数,分析了现代全球变暖背景下台湾东北部海域SST季节与年际异常及其控制因素.季节尺度上,受东亚冬季风的影响,研究海区的冬季SST变化比夏季更为剧烈,冬季SST控制着该海域年均SST和SST季节性的变化.现代器测和古气候记录表明该现象在年际-百年尺度上可能一直存在.年际尺度上,SST异常与MEI指数存在显著的8个月滞后相关性,ENSO(厄尔尼诺—南方涛动,El Ni1o-Southern Oscillation)事件通过东亚冬季风来影响研究海域的SST变化.在历史气候重建中区分气候变化的多尺度性和替代指标的季节性、认识历史气候对ENSO及东亚冬季风的响应特征和机制,这将有助于进一步理解现代全球气候变暖的原因.

关键词：海洋表层温度台湾东北部海域海洋地质东亚冬季风厄尔尼诺-南方涛动

Establishment of an efficient transformation protocol and its application in marine-derived Bacillus strain

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Science China(life Sciences) 2014年第6期57卷 627-635页

作者： liU Yang ZHENG Hua ZHAN GuiHua QIN Wen TIAN li li Wenli Key Laboratory of Marine Drugs Ministry of Education of ChinaSchool of Medicine and PharmacyOcean University of China first institute of {2. State Oceanic Administration qingdao University of Science & Technology

Marine-derived Bacillus strains have been proved to be a very promising source for natural product ***,transformation of environmental strains is much more difficult than that of domesticated ***,we report the development of an efficient and robust electroporation-based transformation system for marine-derived Bacillus marinus B-9987,which is a macrolactin antibiotics producer and a very promising biological control agent against fungal plant *** transformation efficiency was greatly enhanced 103-fold by using unmethylated plasmid to bypass modification-restriction barrier,and using glycine betaine to protect cells from electrical damages during *** of HEPES and 2.mmol L?1MgCl2.further improved the efficiency by additional 2.fold,with a maximum value of 7.1×104 cfu/μg *** demonstrate the feasibility and efficiency of the protocol,a green fluorescent protein reporter system was constructed;furthermore,phosphopantetheinyl transferase gene sfp,which is essential to the biosynthesis of polyketides and nonribosomal peptides,was overexpressed in B-9987,leading to increased production of macrolactin A by about *** addition,this protocol is also applicable to marine-derived Bacillus licheniforms EI-34-6,indicating it could be a reference for other undomesticated Bacillus *** our knowledge,this is the first report regarding the transformation of marine-derived Bacillus strain.

关键词： marine-derived Bacillus marinus electroporation macrolactin green fluorescent protein phosphopantetheinyl transferase

Numerical Solutions of Coupled Nonlinear Schrödinger Equations by Orthogonal Spline Collocation Method

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Communications in Computational Physics 2012年第10期12卷 1392-1416页

作者： Qing-Jiang Meng li-Ping Yin Xiao-Qing Jin Fang-li Qiao Department of Mathematics University of MacaoMacao first institute of {2. StateOceanic AdministrationQingdaoShandong 266061China&College of Physical and Environmental OcanographyOcean University of ChinaQingdaoShandong 266003China Key Laboratory of Marine Science and Numerical Modeling of state oceanic administration&{2. institute of oceanography State Oceanic AdministrationQingdaoShandong 266061China.

In this paper,we present the use of the orthogonal spline collocation method for the semi-discretization scheme of the one-dimensional coupled nonlinear Schrödinger *** method uses the Hermite basis functions,by which physical quantities are approximatedwith their values and derivatives associatedwith Gaussian *** convergence rate with order O(h4+t2.and the stability of the scheme are *** properties are shown in both theory and *** numerical experiments are presented to validate the numerical study under consideration.

关键词： Coupled nonlinear Schrödinger equations orthogonal spline collocation method conservation law

The current situations of green macroalgae and micro-propagules in Pyropia aquaculture of the Subei Shoal in spring of 2.13

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Acta Oceanologica Sinica 2016年第6期35卷 94-100页

作者： liU Xiangqing WANG Zongling FAN Shiliang XIAO Jie li Yan ZHANG Xuelei li Ruixiang WANG Xiaona College of Environmental Science and Engineering Ocean University of China The first institute of {2. State Oceanic Administration Laboratory of Marine Ecology and Environmental Science Qingdao National Laboratory for Marine Science and Technology

To discover the distribution of green algal micro-propagules in the Subei Shoal and clarify the growth of green macroalgae attached on Pyropia aquaculture rafts,an integrated investigation in Pyropia aquaculture area and one cruise in the coastal area of the Subei Shoal were carried out from March to May in *** results showed that green algal micro-propagules were discovered in seawater and sediment during March to *** average quantity of micro-propagules was 2.7 ind./L in surface seawater and 43 ind./g in *** biomass of attached green macroalgae increased in Pyropia aquaculture from March to *** species,including Ulva prolifera,Ulva linza and Blidingia *** found in Pyropia aquaculture *** dominant specie was Blidingia *** the second was *** in *** study indicated that the micro-propagules and macroalgae were existed in the coastal area of the Subei Shoal at the early stage of green *** was the key point to the governance of green tide in China.

关键词： distribution Subei Shoal green algal micro-propagules green macroalgae

Effect of temperature,salinity and irradiance on growth and photosynthesis of Ulva prolifera

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Acta Oceanologica Sinica 2016年第10期35卷 114-121页

作者： XIAO Jie ZHANG Xiaohong GAO Chunlei JIANG Meijie li Ruixiang WANG Zongling li Yan FAN Shiliang ZHANG Xuelei Key Laboratory of Science and Engineering for Marine Ecology and Environment The First Institute of OceanographyState Oceanic AdministrationQingdao 266061China qingdao Environmental Monitoring Station Qingdao 266071China

Intensive Pyropia aquaculture in the coast of southwestern Yellow Sea and its subsequent waste, including disposed Ulva prolifera, was speculated to be one of the major sources for the large-scale green tide proceeding in the Yellow Sea since 2.07. It was, however, unclear how the detached U. prolifera responded and resumed growing after they detached from its original habitat. In this study, we investigated the growth and photosynthetic response of the detached U. prolifera to various temperature, salinity and irradiance in the laboratory. The photosynthetic rate of the detached U. prolifera was significantly higher at moderate temperature levels（14–2.℃）and high salinity（2.–32., with optimum at 2.℃ and 32. Both low（14℃） and highest temperature（40℃）, as well as low salinity（8） had adverse effects on the photosynthesis. Compared with the other Ulva species, U. prolifera showed higher saturated irradiance and no significant photoinhibition at high irradiance, indicating the great tolerance of U. prolifera to the high irradiance. The dense branch and complex structure of floating mats could help protect the thalli and reduce photoinhibition in field. Furthermore, temperature exerted a stronger influence on the growth rate of the detached U. prolifera compared to salinity. Overall, the high growth rate of this detached U. prolifera（10.6%–16.7% d^–1） at a wide range of temperature（5–32.） and salinity（14–32. implied its blooming tendency with fluctuated salinity and temperature during floating. The environmental parameters in the southwestern Yellow Sea at the beginning of green tide were coincident with the optimal conditions for the detached U. prolifera.

关键词： Ulva prolifera green tide photosynthesis growth rate temperature salinity

Community structure and controlled factor of attached green algae on the Porphyra yezoensis aquaculture rafts in the Subei Shoal, China

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Acta Oceanologica Sinica 2015年第8期34卷 93-99页

作者： li Yan XIAO Jie DING Lanping WANG Zongling SONG Wei FANG Song FAN Shiliang li Ruixiang ZHANG Xuelei Ocean University of China first institute of {2. State Oceanic Administration Shantou University

This study analyzes the community structure, the quantity changes of the algae and the effect of important environmental factors and estimates the total biomass of the attached green algae in the survey areas. The study uses data from surveys of the attached green algae on the Porphyra yezoensis aquaculture rafts and data regarding the environmental factors from October 2.10 to April 2.11 in the Subei Shoal. The attached green algae on the rafts included Ulva prolifera, Capsosiphon groenlandicus, U. linza, U. intestinalis, U. clathrata, and U. cornpressa. The biomass changes of the attached green algae exhibited an inverted parabola： the biomass was the highest （14 898 t） in April, and was the second highest （2.034 t） in November; it was lowest in February （only 72. t） and increased sharply from March to April. The species diversity differed significantly among the seasons. In September and October, when the P. yezoensis aquaculture rafts were initially set up, the attached green algae had a high biodiversity, while from December to the next February, a variety of green algae species coexisted on the rafts, although the biomass was low, and from March to April, as the biomass increased sharply, the species diversity dropped to the minimum. During this time, C. groenlandicus was apparently dominant with the maximum biomass proportion up to 80%, while the U. prolifera proportion increased exponentially to 2.% to 40%. The water temperature had a direct regulating effect on the biomass and the species succession of the attached green algae. The estimation of the community dynamics and the biomass of the green algae provided the evidence needed to track the origin of the large-scale green tide in the southern Yellow Sea.

关键词： Porphyra yezoensis aquaculture attached green algae community structure controlled factor SubeiShoal

Bacterial and archaeal community structure of pan-Arctic Ocean sediments revealed by pyrosequencing

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Acta Oceanologica Sinica 2017年第8期36卷 146-152页

作者： liN Xuezheng ZHANG liang liU Yanguang li Yang The first institute of {2. State Oceanic AdministrationQingdao 266061China

This study was to investigate bacterial and archaeal community structure of pan-Arctic Ocean sediments by pyrosequencing. In total, investigation of three marine sediments revealed 15 002.bacterial and 4 362.archaeal operational taxonomic units （OTUs） at the 97% similarity level. Analysis of community structure indicated that these three samples had high bacterial and archaeal diversity. The most relatively abundant bacterial group in Samples CC 1 and R05 was Proteobacteria, while Firmicutes was dominant in Sample BL03. Thaumarchaeota was the most relatively abundant archaeal phylum in Samples CC1 and R05, and the relative abundance of Thaumarchaeota was almost as high as that of Euryarchaeota in Sample BL03. These two phyla accounted for nearly 100% of the archaeal OTUs. 6-Proteobacteria and y-Proteobacteria were the two most relatively abundant classes at Proteobacterial class level, and their relative abundance was more than 60% in Samples CC1 and R05. There were also differences in the top 10 relatively abundant bacterial and archaeal OTUs among the three samples at the 97% similarity, and only 12.core bacterial OTUs were detected. Overall, this study indicated that there were distinct microbial communities and many unique OTUs in these three samples.

关键词： Arctic sediment microbial community diversity pyrosequencing

Temperature inversion in the Huanghai Sea bottom cold water in summer

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Acta Oceanologica Sinica 2013年第3期32卷 42-47页

作者： YUAN Dongliang li Yao QIAO Fangli ZHAO Wei Key Laboratory of Ocean Circulation and Waves Chinese Academy of Sciences institute of Oceanology Chinese Academy of Sciences Graduate University of Chinese Academy of Sciences first institute of {2. State Oceanic Administration

Using conductivity-Temperature-depth data of a recent cruise during July 2.-2., 2.08 and historical data, it is found that temperature inversions occur from time to time in the Huanghai Sea（Yellow Sea） cold water mass （HSCWM） in summer. The temperature inversions are produced by the movement of the fresh and cold HSCWM masses above the warm and saline Huanghai Sea Warm Current water at the central bottom of the Huanghai Sea Trough. The non-homogeneous profiles of the temperature and the salinity suggest that vertical mixing in the HSCWM, which is of great importance to the circulation in the Huanghai Sea in summer, is weak. Trajectories of satellite-tracked surface drifters suggest that waters in the northern reach of the Huanghai Sea move southward along the 40-50 m isobaths and descend into the southern Huanghai Sea to form the western core of the HSCWM.

关键词： temperature inversion Huanghai Sea bottom cold water Huanghai Sea cold water mass sum-mer