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Chemical Research in Chinese Universities 2008年第6期24卷 771-777页

作者： li zhi-li Institute of Basic Medical Sciences Chinese Academy of Medical Sciences ＆ Peking Union Medical College Beijing 100005 P. R. China

Escherichia coli 3-Deoxy-D-manno-octulosonate 8-phosphate（KDO8P） synthase catalyzed the condensation reaction between D-arabinose 5-phosphate（A5P） and phosphoenolpyruvate（PEP） to form KDO8P and inorganic phosphate（Pi）. The noncovalent tetrameric association ofKDO8P synthase was observed and dissociated in gas phase by means of electrospray ionization mass spectrometry under the very ＂soft＂ conditions. The results indicate that PEP-bound enzyme generated abundant tetrameric species as well as monomeric species at the ＂soft＂ conditions, whereas, the unbound enzyme favored the formation of a dimeric species. The mass spectra of the mixture of the enzyme with one of substrates, PEP, and A5P or one of products, KDO8P and Pi show that the complex of the unbound enzyme with PEP or Pi was prone to the formation of a monomeric species, whereas, that of the unbound enzyme with A5P or KDO8P was similar to the unbound enzyme. The intensity of the dimeric species increased with the increase of temperature at a collision voltage of 10 V. Taken together, the results presented here suggest that mass spectrometry will be a powerful tool to explore subtile conformational changes and/or subunit-subunit interactions of multiprotein assembly induced by ligand-binding and/or the changes of environmental conditions.

关键词： Electrospray ionization mass spectrometry 3-Deoxy-D-manno-octulosonate 8-phosphate(KDO8P) syn- thase Oligomeric stability

Decoherence of a Double Quantum Dot Charge Qubit Approached with Redfield Equation

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Communications in Theoretical Physics 2009年第2期51卷 345-348页

作者： li Zeng-Zhao li zhi-Qiang Department of Physics and Institute of Modern Physics Ningbo University Ningbo 315211 China

In this paper, we investigate the decoherence time of a double quantum dot （DQD） charge qubit in three kinds of baths through solving dynamics of the qubit. The dynamics of the qubit is investigated with Redfield master equation. It is shown that the decoherence time of the qubit in Ohmic bath has the same order of magnitude as the experiments reported. When the environment is modeled with the supra-Ohmic bath the decoherence time of the qubit is shorter than the experimental result. And when modeled with the sub-Ohmic bath the decoherence time of the qubit is longer than the experimental result.

关键词： quantum dot decoherence redfield equation

Tensile reliability analysis for gravity dam foundation surface based on FEM and response surface method

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Water Science and Engineering 2010年第2期3卷 233-240页

作者： Tong-chun li Dan-dan li zhi-qiang WANG College of Water Conservancy and Hydropower Engineering Hohai University Nanjing 210098 P. R. China

In this study, the limit state equation for tensile reliability analysis of the foundation surface of a gravity dam was established. The possible crack length was set as the action effect and allowable crack length was set as the resistance in the limit state. The nonlinear FEM was used to obtain the crack length of the foundation surface of the gravity dam, and the linear response surface method based on the orthogonal test design method was used to calculate the reliability, providing a reasonable and simple method for calculating the reliability of the serviceability limit state. The Longtan RCC gravity dam was chosen as an example. An orthogonal test, including eleven factors and two levels, was conducted, and the tensile reliability was calculated. The analysis shows that this method is reasonable.

关键词： tensile reliability foundation surface of gravity dam nonlinear FEM," response surface method," Longtan RCC gravity dam

Studies on Ion-molecule Reaction of Disubstituted Benzene with Ion System of Acetyl Chloride in Gas Phase

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Chemical Research in Chinese Universities 2000年第4期16卷 334-340页

作者： li zhi-li (Shandong Non-metallic Material Research Institute, Jinan 250031, P. R. China) 山东省非金属材料所山东济南 250031

The ion-molecule reactions of disubstituted benzenes with the ion system of acetyl chloride under the chemical ionization condition were examined and the fragmentation reactions of the adduct ions formed by the ion-molecule reactions were studied by using collision-induced dissociation technique. It was found that the electron-releasing groups favored the adduct reactions and the electron-withdrawing groups did not. The position and properties of substituting groups had an effect on the relative abundance of the adduct ions. The fragmentation reaction of the adduct ions formed by ortho-benzene diamine with the acetyl ion was similar to the reductive alkylation reaction of amine in condensed phase.

关键词： Disubstituted benzene Ion-molecule reaction Collision-induced dissociation technique Adduction

Clinical Features and Treatment of Bronchogenic Cyst in Adults

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Chinese Medical Sciences Journal 2009年第1期24卷 60-63页

作者： Hong-sheng liu Shan-qing li zhi-li Cao zhi-yong Zhang Hua Ren Department of Thoracic Surgery Peking Union Medical College Hospital Chinese Academy of Medical Sciences ＆ Peking Union Medical College Beijing 100730 China

Objective To investigate the clinical features and management of bronchogenic cyst in the adults. Methods We retrospectively reviewed 50 patients admitted to our hospital with histopathologically proved bronchogenic cyst from January 1983 to December 2007. Of all the patients, 28 were male and 22 were female, with an average age of 36.9 (range, 18 to 64) years. The symptoms, location of the cysts, imaging evaluation, surgical treatment manner, and outcome of these patients were analyzed. Results Symptoms were present in 33 of the 50 patients, and cough was the most common symptom. Thirteen patients presented with complications: hemoptysis, infected cyst, dysphagia, paralysis, and hoarseness. The locations of the cysts included the mediastinum (28 cases), pulmonary parenchyma (12 cases), hilar area (3 cases), visceral pleura (1 case), and some rare locations including the intestinal mesentery (1 case), retroperitoneum (1 case), adrenal gland (1 case), neck (2 cases), and dura matter of the cervical verte-brae (1 case). Chest X-ray was performed in 36 patients and computed tomography (CT) was performed in 41 patients. The bronchogenic cyst in CT was characterized as a round, well circumscribed, unilocular mass, with density ranging from that of water to high density (0-50 Hu). As for treatment, complete resection of the bronchogenic cyst was performed in 47 (94%) patients, subtotal resection was performed in 3 (6%) patients. Open surgery was performed in 45 (90%) patients, and thoracoscopy (video-assisted thoracic surgery) was performed in 5 (10%) paitients. Of the 12 patients with intrapulmonary cyst, 11 patients underwent lobectomy and 1 patient underwent wedge resection. Postoperative sequelae occurred in 2 patients, 1 with persistent air leakage and 1 with hoarseness. All patients were proved with bronchogenic cyst pathologically. The average follow-up period was 6.5 years (range, 4 months to 10 years), and no late sequelae or recurrence of the cyst occurred. Conc

关键词： bronchogenic cyst lung mediastinum thoracoscopy

Effect of Simulated Microgravity and its Associated Mechanism on Pulmonary Circulation in Rats

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Biomedical and Environmental Sciences 2013年第2期26卷 118-127页

作者： li Tian zhi YUAN Ming CHEN Zhen Hong GUO Ying Hua KANG Chun Yan WANG Jing Yu li zhi li WANG De Sheng WANG Hui Juan YUAN Min liU Chang Ting Nanlou Respiratory Diseases Department Chinese PLA General Hospital State KeyLaboratory of Space Medicine Fundamentals and Application China Astronaut Research and Training Center

Objective To study the effect of Simulated Microgravity and its Associated Mechanism on Pulmonary Circulation in Rats. Methods Rat tail-suspension model was used to simulate the physiological effects of microgravity and changes in pulmonary blood vessel morphology, pulmonary arterial and venous blood pressure, pulmonary vascular resistance, pulmonary vasomotoricity, as well as the regulation of pulmonary circulation by cytokines produced and released by the lung of rats were measured. Results The walls of pulmonary blood vessels of rats were thickened, and the pulmonary artery was reconstructed with increased pulmonary vascular resistance. The pulmonary blood vessels of rats became more prone to dilation as contractions increased. Rat epithelial Adrenomedulin gene transcription and protein expression were upregulated. The level of basic fibroblast growth Factor of rat was also elevated. Conclusion Findings from the present study on rats revealed that the microgravity can affect pulmonary blood vessel structure, pulmonary arterial pressure, and pulmonary blood vessel self-regulation and cytokine production.

关键词： Rat Simulated microgravity Changes in pulmonary circulation Regulations of pulmonarycirculation

Detection and Characterization of Non-covalent Complex between Lappaconitine and β-Cyclodextrin by Electrospray Ionization Tandem Mass Spectrometry

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Chinese Chemical Letters 2006年第1期17卷 65-68页

作者： Qing Xuan XU li li Hao YUE zhi Qiang liU Ming Quart GUO Shu Ying liU Laboratory of New Drug Research and Mass Spectrometry Changchun Institute of Applied Chemistry Chinese Academy of Sciences Changchun 130022

The non-covalent complexes between lappaconitine （LA） and β-cyclodextrin （β-CD） have been detected and characterized by electrospray ionization combined with ion trap tandem mass spectrometry （ESI-MSn）. The experimental results showed that only 1：1 non-covalent complex can be formed in different starting molar ratios of LA to β-CD. Furthermore, the diagnostic fragmentation of the β-CD-LA complex, with a significant contribution of covalent fragmentation of LA leaving the N-acetyl anthranoyl （AN） moiety inserted to β-CD, provided the convincing evidence for the formation of non-covalent complex between LA and β-CD and the cite of LA molecule included to cavity of β-CD assigned to AN residue.

关键词： Lappaconitine β-cyclodextrin non-covalent complex ESI-MS CID.

Public Awareness and Mask Usage during the COVID-19 Epidemic:A Survey by China CDC New Media

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Biomedical and Environmental Sciences 2020年第8期33卷 639-645页

作者： SUN Cheng Xi HE Bin MU Di li Pei Long ZHAO Hong Ting li zhi li ZHANG Mu li FENG Lu Zhao ZHENG Jian Dong CHENG Ying CUI Ying li Zhong Jie Shandong Center for Disease Control and Prevention Jinan 250014ShandongChina Health Communication Center Chinese Centre for Disease Control and PreventionBeijing 102206China Division of Infectious Disease Key Laboratory of Surveillance and Early-warning on Infectious DiseaseChinese Centre for Disease Control and PreventionBeijing 102206China Division of Epidemiology National Center for AIDS/STD Control and PreventionChinese Center for Disease Control and PreventionBeijing 102206China

An online survey conducted March 18–19,2020 on the official China CDC WeChat account platform was used to evaluate the effect of public education about masks usage during the new coronavirus disease 2019(COVID-19)*** nationals older than 18 were eligible for the *** survey collected 5,761 questionnaires from the 31 provinces,municipalities,and autonomous regions of China's Mainland.99.7%and 97.2%of the respondents answered correctly that respiratory droplets and direct contact were the main transmission routes.73.3%of the respondents considered COVID-19 to be‘serious’or‘very serious’.

关键词： mainland autonomous education

Evaluation of Sensitivities and Specificities of SARS-CoV Detection by Real-time Quantitative Reverse Transcription-PCR Assays

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Virologica Sinica 2009年第3期24卷 187-193页

作者： li-li XU zhi-hong HU Hua-lin WANG Xiao HAN Fei DENG State Key Laboratory of Virology Wuhan Institute of Virology Chinese Academy of Sciences Wuhan 430071 China

The etiological agent of severe acute respiratory syndrome(SARS) was identified as a new coronavirus,termed SARS-CoV. Establishment of an efficient and sensitive diagnostic system of SARS-CoV genetic materials is crucial for SARS control. In this study,we quantified SARS-CoV mRNAs in both infected cell culture lysate and in supernatant by using Real-time quantitative revere transcription-PCR based on EvaGreenTM dye and Taqman-MGB probes. For extensive evaluation of sensitivities and specificities,13 pairs of primers and 4 probes were designed based on different genes of SARS-CoV. Glyceraldehydes-3-phosphate dehydrogenase(GAPDH) was selected as the internal control gene. Results showed that S-gene-specific PCR was the most sensitive for detection,but because of its sequence variability in the different viral strains,primers and a probe based on the N gene were suitable substitutions. Meanwhile,we found the mRNA concentrations in cell culture lysates were much higher than in cell supernatant and facilited more sensitive detection of the SARS-CoV.

关键词： SARS-CoV Sensitivities Specificities Evaluation

Research progress and strategies for multifunctional rapeseed： A case study of China

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Journal of Integrative Agriculture 2016年第8期15卷 1673-1684页

作者： FU Dong-hui JIANG ling-yan Annaliese S Mason XIAO Mei-li ZHU Long-rong li li-zhi ZHOU Qing-hong SHEN Chang-jian HUANG Chun-hui Key Laboratory of Crop Physiology Ecology and Genetic Breeding Ministry of Education/Agronomy College Jiangxi Agricultural University Nanchang 330045 P.R.China Department of Biochemistry University of Missouri Columbia 65211 USA Department of Plant Breeding IFZ Research Centre for Biosystems Land Use and Nutrition Justus Liebig University Heinrich- Buff-Ring 26-32 Giessen 35392 Germany Jiangxi Province Key Laboratory of Animal Nutrition/Engineering Research Center of Feed Development Jiangxi Agricultural University Nanchang 330045 P.R. China linli County Rural Practical Technology Research Institute Changde 415000 P.R. China

financially supported by the Key R＆D Program of Jiangxi Province of China （20152ACF60010）;the Key Projects of Hunan Science and Technology Support Program of China （Application research of Guiye A male sterile line）;the Research Project of Humanities and Social Sciences in Universities of Jiangxi Province, China（JC1315）;supported by Emmy Noether DFG grant MA 6473/1-1

关键词： multifunctional rapeseed sightseeing rapeseed remediation of cadmium pollution rapeseed oil fodder vegetables