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Modeling and Analyzing for a Novel Continuum Model Considering Self-Stabilizing Control on Curved Road with Slope

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Computer Modeling in Engineering & Sciences 2022年第6期131卷 1815-1830页

作者： li lei Zihao Wang Yong Wu School of Energy and Power Engineering Shandong UniversityJinan250061China School of Control Science and Engineering Shandong UniversityJinan250061China Department of Logistics Management Ningbo University of Finance and EconomicsNingbo315175China

It is essential to fully understand master the traffic characteristics of the self-stabilizing control effect and roadcharacteristics to ensure the regular operation of transportation. Traffic flow on curved roads and slopes is irregularand more complicated than that on the straight road. However, most of the research only considers the effect ofself-stabilizing in the straight road. This study attempts to bridge this deficiency from the following three aspects.First, we review the potential influencing factors of traffic flow stability, which are related to the vehicle’s steadyvelocity, history velocity, and the turn radius of the road and the slope of the road. Based on the above review,an extended continuum model accounting for the self-stabilizing effect on a curved road with slope is proposed.Second, the linear stability criterion of the new model is derived by applying linear stability theory, and the neutralstability curve is obtained in detail. The modified KdV equation describing the evolution characteristics of trafficcongestion is derived by using the nonlinear analysis method. Upon the theoretical analysis, the third aspectfocuses on simulating the self-stabilizing effect under different slopes and radius, which demonstrates that theself-stabilizing effect is conducive to reducing congestion of the curved road with slope.

关键词： Traffic flow KdV equation self-stabilizing effect gradient highway curved road

Developing a fluorescence substrate for HRP-based diagnostic assays with superiorities over the commercial ADHP

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Chinese Chemical Letters 2024年第6期35卷 439-444页

作者： Zhichao Zhou Fuqian Chen Xiaotong Xia Dong Ye Rong Zhou lei li Tao Deng Zhenhua Ding Fang liu Department of Radiation Medicine Guangdong Provincial Key Laboratory of Tropical Disease ResearchSchool of Public HealthSouthern Medical UniversityGuangzhou 510515China School of Pharmaceutical Sciences Guangzhou University of Chinese MedicineGuangzhou 510006China Artemisinin Research Center Guangzhou University of Chinese MedicineGuangzhou 510006China State Key Laboratory of Respiratory Disease the First Affiliated Hospital of Guangzhou Medical UniversityGuangzhou Medical UniversityGuangzhou 510120China Guangzhou Laboratory Bio-IslandGuangzhou 510030China Nanshan Pharmaceutical Innovation Research Institute of Guangdong Province Foshan 528225China

The combination of horseradish peroxidase(HRP)and a fluorescence substrate has been attracting great interests in developing sensitive biochemical analysis and immunoassays.10-Acetyl-3,7-dihydroxyphenoxazine(ADHP or Amplex red)is the most sensitive fluorogenic substrate known for HRP in current market,however,it suffers from some drawbacks,such as non-specific reactivity to carboxylesterase and limited fluorescence stability.In the present study,a novel HRP substrate10-cyclopropylcarbonyl-dichloro-dihydroxyphenoxazine(AR-2),has been prepared,which exhibited improved sensitivity than ADHP in sensing HRP.Moreover,the fluorescence of AR-2/HRP demonstrated improved tolerance to physiological relevant p H fluctuation as compared to ADHP/HRP.Successful detection of uric acid/urate oxidase reaction indicated excellent application prospect of AR-2/HRP for monitoring H_(2)O_(2)-generating biochemical reactions.More interestingly,an enzyme-linked immunosorbent assay(EliSA)using AR-2 as the fluorescence reporter has been successfully used in detecting IgG against severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)from human serum samples.Overall,AR-2 exhibits improved performances over the commercial ADHP,which will be an ideal alternative to ADHP in HRP-based fluorescence biochemical analysis and immunoassays.

关键词： Fluorescence immunoassay HRP fluorescence substrate Hydroresorufin Amplex red/ADHP SARS-CoV-2

Application and Prospect of Platelet Multi-omics Technology in Study of Blood Stasis Syndrome

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Chinese Journal of Integrative Medicine 2022年第2期28卷 99-105页

作者： li Ying SUN Ming-qian li lei ZHANG Ye-hao MIAO Lan liU Jian-xun Beijing Key Laboratory of Pharmacology of Chinese Materia Region Xiyuan HospitalChina Academy of Chinese Medical SciencesNational Clinical Research Center for Chinese Medicine CardiologyBeijing(100091)China

The abnormality of platelet function plays an important role in the pathogenesis and evolution of blood stasis syndrome(BSS).The explanation of its mechanism is a key scientific issue in the study of cardiovascular and cerebrovascular diseases and treatment.System biology technology provides a good technical platform for further development of platelet multi-omics,which is conducive to the scientific interpretation of the biological mechanism of BSS.The article summarized the pathogenesis of platelets in BSS,the mechanism of action of blood activating and stasis resolving drugs,and the application of genomics,proteomics,and metabonomics in platelet research,and put forward the concept of"plateletomics in BSS".Through the combination and cross-validation of multi-omics technology,it mainly focuses on the clinical and basic research of cardiovascular and cerebrovascular diseases;through the interactive verification of multi-omics technology and system biology,it mainly focuses on the platelet function and secretion system.The article systematically explains the molecular biological mechanism of platelet activation,aggregation,release,and other stages in the formation and development of BSS,and provides a new research idea and method for clarifying the pathogenesis of BSS and the mechanism of action of blood activating and stasis resolving drugs.

关键词： blood stasis syndrome platelet transcriptomics metabonomics proteomics

A Bifunctional-Blocker-Aided Hybridization Chain Reaction lighting-Up Self-calibrating Nanocluster Fluorescence for Reliable Nucleic Acid Detection

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Journal of Analysis and Testing 2024年第2期8卷 160-169页

作者： Dan Zhang Guobao Zhou Hongyan Yang Yi Wang lijun Shen Yuxuan Qiu lei li Longhua Guo College of Chemistry and Materials Science Zhejiang Normal UniversityJinhua321004China Jiaxing Key Laboratory of Molecular Recognition and Sensing College of BiologicalChemical Sciences and EngineeringJiaxing UniversityJiaxing314001China College of life Sciences and Medicine Zhejiang Sci-Tech UniversityHangzhou314000China

In this work,we proposed a ratiometric silver nanoclusters(AgNCs)fluorescent assay by designing a bifunctional-blockeraided hybridization chain reaction(HCR).Hairpin probe 1(HP1)containing two special DNA fragments(5′-CAC CGC T-3′and 5′-ATT TGC CTT TTG GGG ACG GATA-3′)at two terminals creates a red-emitting AgNC nucleation sequence(rNS,5′-CAC CGC TAT TTG CCT TTT GGG GAC GGATA-3′).We found that the presence of a toehold fragment(5′-TGCCC-3′)in HP1 could silence the rNS.Upon the addition of a target nucleic acid,HCR of HP1 and hairpin probe 2(HP2)could be initiated,resulting in the formation of long chain of DNA duplexes with multibranched rNS.As the toehold fragment in HP1participated in generating duplexes,a strong emission of rNS-templated AgNCs was observed at 670 nm.More significantly,a bifunctional blocker was introduced not only to reduce the background red-emitting fluorescence but also to play as an internal green-emitting AgNCs nucleation sequence.On the one hand,the blocker could increase the signal-to-noise-ratio of the constructed biosensor,and on the other hand,the blocker also helped to prepare ratiometric HCR-AgNCs assay with self-calibrating ability to strengthen its reproducibility.Compared with the traditional HCR-AgNCs sensors,the developed ratiometric assay based on the bifunctional-blocker-aided HCR has higher reliability,which is important for the fabrication of biosensors in various fields for practical biosensing applications.

关键词： Ratiometric fluorescence Hybridization chain reaction Silver nanocluster Biosensor Bifunctional blocker

Establishment of acquired tracheoesophageal fistula using a modified magnetic compression technique in rabbits and its postmodeling evaluation

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World Journal of Gastrointestinal Surgery 2024年第5期16卷 1385-1394页

作者： Han Meng Fu-Yao Nan Na Kou Qin-Yan Hong Ming-Sheng Lv Ju-Bo li Bao-Jie Zhang Hang Zou lei li Hong-Wu Wang Respiratory Disease Center Dongzhimen HospitalBeijing University of Chinese MedicineBeijing 100029China The First Clinical Medical College Beijing University of Chinese MedicineBeijing 100700China Department of Animal Experimental Center National Center for CardiovascularFuwai HospitalBeijing 100037China

BACKGROUND Previous studies have validated the efficacy of both magnetic compression and surgical techniques in creating rabbit tracheoesophageal fistula(TEF)models.Magnetic compression achieves a 100%success rate but requires more time,while surgery,though less frequently successful,offers rapid model establishment and technical maturity in larger animal models.AIM To determine the optimal approach for rabbit disease modeling and refine the process.METHODS TEF models were created in 12 rabbits using both the modified magnetic compression technique and surgery.Comparisons of the time to model establishment,success rate,food and water intake,weight changes,activity levels,bronchoscopy findings,white blood cell counts,and biopsies were performed.In response to the failures encountered during modified magnetic compression modeling,we increased the sample size to 15 rabbit models and assessed the repeatability and stability of the models,comparing them with the original magnetic compression technique.RESULTS The modified magnetic compression technique achieved a 66.7%success rate,whereas the success rate of the surgery technique was 33.3%.Surviving surgical rabbits might not meet subsequent experimental requirements due to TEF-related inflammation.In the modified magnetic compression group,one rabbit died,possibly due to magnet corrosion,and another died from tracheal magnet obstruction.Similar events occurred during the second round of modified magnetic compression modeling,with one rabbit possibly succumbing to aggravated lung infection.The operation time of the first round of modified magnetic compression was 3.2±0.6 min,which was significantly reduced to 2.1±0.4 min in the second round,compared to both the first round and that of the original technique.CONCLUSION The modified magnetic compression technique exhibits lower stress responses,a simple procedure,a high success rate,and lower modeling costs,making it a more appropriate choice for constructing TEF models in rabbits.

关键词： Tracheoesophageal fistula Modified magnetic compression technique Post-modeling evaluation Pneumonia Malnutrition

The effect of secondary phase on corrosion behaviors of the titanium–zirconium–molybdenum alloy

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Tungsten 2024年第2期6卷 342-354页

作者： Song-Wei Ge Ping Hu Jie Deng Shi-lei li Hai-Rui Xing Jia-Yu Han Xing-Jiang Hua li Wang Jun-Zhou Yang Bo Jin Wen Zhang Kuai-She Wang School of Metallurgy Engineering Xi’an University of Architecture and TechnologyXi’an 710055China National and Local Joint Engineering Research Center for Functional Materials Processing Xi’an University of Architecture and TechnologyXi’an 710055China Western Xinxing Rare and Precious Metals Co. Ltd.Shangluo 726000China Northwest Institute for Non-Ferrous Metal Research Xi’an 710016China

As the range of applications for molybdenum and its alloys has expanded,the corrosive environment for molybdenum alloys has become more demanding.In the past,the content of doping elements has been studied to investigate their infl uence on the corrosion performance of titanium–zirconium–molybdenum(TZM)alloys.In this paper,it is considered that the second phase in the alloy is the main factor aff ecting the corrosion performance of TZM alloys.By comparing the corrosion behavior of molybdenum metal and TZM alloy,the eff ect of the secondary phase on corrosion behaviors of TZM alloy has been investigated.The results show that the second phase reduces the corrosion resistance of the TZM alloy.The potential of the second phase is 73.7 mV higher than that of the Mo matrix,which contributes to the formation of microscopic electric couples.Under the action of microscopic electric couples,pitting corrosion is preferentially formed at the interface between the second phase and the matrix,which accelerates the corrosion of the matrix.This paper provides a theoretical basis for the application of TZM alloys in corrosive environments.

关键词： Secondary phase Electrochemical corrosion Titanium–zirconium–molybdenum(TZM) Galvanic corrosion Corrosion resistance

A repertoire of intronic lariat RNAs reveals tissue-specific regulation and target mimicry potential in plants

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Science China(life Sciences) 2024年第6期67卷 1280-1291页

作者： Yong Zhang Xiaotuo Zhang Qi Tang lei li Ting Jiang Yixiao Fang Hong Zhang Jixian Zhai Guodong Ren Binglian Zheng State Key Laboratory of Genetic Engineering Institute of Plant BiologySchool of Life SciencesFudan UniversityShanghai 200438China Department of Biology School of Life SciencesSouthern University of Science and TechnologyShenzhen 518055China

Lariat RNA is concomitantly produced by excised intron during RNA splicing,which is usually debranched by DBR1,an RNA debranching enzyme.However,increasing evidence showed that some lariat RNA could escape debranching.little is known about how and why these lariat RNAs could be retained.By comparing the atlas of lariat RNAs between the non-dividing cell(mature pollen)and three actively dividing tissues(young shoot apex,young seeds,and young roots),we identified hundreds to thousands of lariat RNA naturally retained in each tissue,and the incidence of lariat RNA retention is much less in shoot apex while much more in pollen.Many lariat RNAs derived from the same intron or different lariat RNAs from the same pre-m RNA could be retained in one tissue while degraded in the other tissues.By deciphering lariat RNA sequences,we identified an AG-rich(RAAAAVAAAR)motif and a UC-rich(UCUCUYUCUC)motif for pollen-specific and the other three tissues-retained lariat RNAs,respectively.Reconstitution of the pollen-specific AG-rich motif indeed enhanced lariat RNA retention in plants.Biologically,hundreds of lariat RNAs harbored mi RNA binding sites,and dual-luciferase reporter assay showed that these natural lariat RNAs had the potential to protect expression of mi RNA target genes.Collectively,our results uncover that selective retention of lariat RNA is an actively regulatory process,and provide new insights into understanding how lariat RNA metabolism may impact mi RNA activity.

关键词： intron turnover lariat RNA miRNA RNA splicing DBR1

Pollution Dispersion in Urban Street Canyons with Green Belts

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Computer Modeling in Engineering & Sciences 2022年第8期132卷 661-679页

作者： Xiaoxuan Zhu Xueyan Wang li lei Yuting Zhao School of Energy and Power Engineering Shandong UniversityJinan250061China CNPC Ji Chai Power Equipment Company Jinan250306China

In this study,numerical simulations were used to explore the effects of roadside green belt,urban street spatial layout,and wind speed on vehicle exhaust emission diffusion in street canyon.The diffusion of different sized particles in the street canyon and the influence of wind speed were investigated.The individual daily average pollutant intake was used to evaluate the exposure level in a street canyon microenvironment.The central and leeward green belts of the road were the most conducive to the diffusion of pollutants,while the positioning of the green belts both sides of a road was least conducive to the diffusion of pollutants.Pollutant levels increased with increasing canopy height,canopy width,and decreasing tree spacing,with optimal values of 12 m,7 m,and 0.4 H,respectively.This provides protection from pollution for low-rise residents and pedestrians.The results presented here can be used to improve the air quality of the street microenvironment and provide a basis for the renovation of old street buildings.

关键词： Street canyons roadside green belts pollutant exposure particulate matter fluent software

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人类免疫缺陷病毒感染相关血脂异常研究进展

中华内科杂志 2024年第3期63卷 303-309页

作者：李磊马萍天津市第二人民医院感染二科天津300192

人类免疫缺陷病毒(HIV)感染者常见脂质代谢异常,导致该类人群心血管疾病患病率增加。HIV全病程管理使包括心血管疾病在内的非艾滋定义性疾病(NAD)得到关注,抗反转录病毒疗法(ART)与血脂异常的关系以及不同类别的ART药物对血脂水平的影... 详细信息

人类免疫缺陷病毒(HIV)感染者常见脂质代谢异常,导致该类人群心血管疾病患病率增加。HIV全病程管理使包括心血管疾病在内的非艾滋定义性疾病(NAD)得到关注,抗反转录病毒疗法(ART)与血脂异常的关系以及不同类别的ART药物对血脂水平的影响差异需要引起重视。本文基于临床证据和国内外文献、指南及专家共识,对HIV感染者/AIDS患者(PLWHA)血脂异常的危险因素与诊疗管理方式进行总结和展望。

关键词： HIV 血脂异常抗反转录病毒治疗血脂管理