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Transplantation of human induced pluripotent stem cell derived keratinocytes accelerates deep second-degree burn wound healing

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World Journal of Stem Cells 2023年第7期15卷 713-733页

作者： li-jun wu Wei lin Jian-Jiang liu Wei-Xin Chen Wen-jun He Yuan Shi Xiao liu Ke li Department of Plastic and Aesthetic Surgery The Second Affiliated Hospital of Soochow UniversitySuzhou 215004Jiangsu ProvinceChina Department of Burn and Plastic Surgery The First Affiliated Hospital of Soochow UniversitySuzhou 215006Jiangsu ProvinceChina

BACKGROUND Current evidence shows that human induced pluripotent stem cells(hiPSCs)can effectively differentiate into keratinocytes(KCs),but its effect on skin burn healing has not been *** To observe the effects of hiPSCs-derived KCs transplantation on skin burn healing in mice and to preliminarily reveal the underlying *** An analysis of differentially expressed genes in burn wounds based on GEO datasets GSE140926,and GSE27186 was established.A differentiation medium containing retinoic acid and bone morphogenetic protein 4 was applied to induce hiPSCs to differentiate into *** expression of KCs marker proteins was detected using immunofluorescence staining.A model of a C57BL/6 mouse with deep cutaneous second-degree burn was created,and then phosphate buffered saline(PBS),hiPSCs-KCs,or hiPSCs-KCs with knockdown of COL7A1 were injected around the wound *** wound healing,re-epithelialization,engraftment of hiPSCs-KCs into wounds,proinflammatory factor level,and the NF-κB pathway proteins were assessed by hematoxylin-eosin staining,carboxifluorescein diacetate succinimidyl ester(CFSE)fluorescence staining,enzyme linked immunosorbent assay,and Western blotting on days 3,7,and 14 after the injection,***,the effects of COL7A1 knockdown on the proliferation and migration of hiPSCs-KCs were confirmed by immunohistochemistry,EdU,Transwell,and damage repair *** HiPSCs-KCs could express the hallmark proteins of ***7A1 was down-regulated in burn wound tissues and highly expressed in *** of hiPSCs-KCs into mice with burn wounds resulted in a significant decrease in wound area,an increase in wound re-epithelialization,a decrease in proinflammatory factors content,and an inhibition of NF-κB pathway activation compared to the PBS *** in vitro assay showed that COL7A1 knockdown could rescue the inhibition of hiPSCs-KCs proliferation and migration,providing further evidence that COL7A

关键词： Induced pluripotent stem cell Keratinocytes Cell transplantation Burn wound healing COL7A1

Organoids of liver diseases: From bench to bedside

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World Journal of Gastroenterology 2019年第16期25卷 1913-1927页

作者： li-jun wu Zi-Yan Chen Yi Wang jun-Gang Zhao Xiao-Zai Xie Gang Chen Department of Hepatobiliary Surgery The First Affiliated Hospital Wenzhou Medical University Research Center of Evidence-Based Medicine and Clinical Epidemiology School of Public Health and Management Wenzhou Medical University

Understanding the occurrence, development, and treatment of liver diseases is the main goal of hepatopathology research. liver diseases are not only diverse but also highly heterogeneous among individuals. At present, research on liver diseases is conducted mainly through cell culture, animal models, pathological specimens, etc. However, these methods cannot fully reveal the pathogenic mechanism and therapeutic characteristics of individualized liver *** advances in three-dimensional cell culture technology(organoid culture techniques) include pluripotent stem cells and adult stem cells that are cultured in vitro to form self-organizing properties, making it possible to achieve individualized liver disease research. This review provides a comprehensive overview of the development of liver organoids, the existing and potential applications of liver regenerative medicine, the pathogenesis of liver disease heterogeneity, and drug screening.

关键词： Organoids liver diseases Individualized research liver cancer Threedimensional cell culture liver organoids

Two new lanostane triterpenoids from Abies sachalinensis

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Chinese Chemical Letters 2008年第12期19卷 1447-1449页

作者： Hui Yuan Gao Jian Huang Bo Hang Sun Masonori Kuroyanagi li jun wu School of Traditional Chinese Medicines Shenyang Pharmaceutical University Shenyang 110016 China Faculty of life and Environment School Prefectural University of Hiroshima Hiroshima 727-0023 Japan

The continuous chemical investigation on the ethyl acetate （EtOAc） soluble fraction of the MeOH extract afforded two new lanostane triterpenoid derivatives including one with a rearranged lanostane skeleton. They were identified as 3,4-seco-8-（14→13R）abeo-17,13-friedo-9β-lanosta-4（28）, 7,14（30）,24-tetraen-26,23-olide-23-hydroxy-3-oic acid （1） and 7,14-mariesa- dien-3oL-hydroxy-25-methoxy-26-oic acid （2）. Structural determination of these compounds were carried out by the spectral studies especially by the two digital （2D）-NMR and high-resolution MS experiences.

关键词： Abies sachalinensis Rearranged lanostane 17,13-Fdedolanostane

Pion-photon and kaon-photon transition distribution amplitudes in the Nambu–Jona-Lasinio model

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Chinese Physics C 2024年第8期48卷 94-103页

作者： Jin-li Zhang jun wu Department of Mathematics and Physics Nanjing Institute of TechnologyNanjing 211167China National Laboratory of Solid State Microstructures Department of PhysicsNanjing UniversityNanjing 210093China

The Nambu–Jona-Lasinio model is utilized to investigate the pion-and kaon-photon leading-twist transition distribution amplitudes using proper time ***,the properties of the vector and axial vector pion-photon transition distribution amplitudes are examined,and the results meet the desired *** study involves sum rule and polynomiality *** vector and axial vector pion-photon transition form factors that are present in theπ^(+)→γe^(+)νprocess are the first Mellin moments of the pion-photon transition distribution *** vector transition form factor originates from the internal structure of hadrons,the axial current can be coupled to a pion,this pion is virtual,and its contribution will be present independently of the external *** kaon transition form factors are *** vector form factor's value at zero momentum transfer is determined by the axial anomaly,while this is not the case for the axial *** vector and axial form factors,as well as the neutral pion vector form factor F_(πγγ)(t),are *** to our findings,the pion axial transition form factor is harder than the vector transition form factor and harder than the electromagnetic form *** also discuss the link betweenπ−γandγ−πtransitions distribution amplitudes.

关键词： transition form factors NJL model transition distribution amplitudes

Characterization of the First Genome of Porcine mastadenovirus B(HNU1 Strain) and Implications on Its Lymphoid and Special Origin

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Virologica Sinica 2020年第5期35卷 528-537页

作者： Shu-Jing liu Qiong Wang Ting-Ting li Si-Hua Zhang Jin-Yan li li-jun wu Ye Qiu Xing-Yi Ge Hunan Provincial Key Laboratory of Medical Virology Institute of Pathogen Biology and ImmunologyCollege of BiologyHunan UniversityChangsha 410082HunanChina Hubei Animal Disease Prevention and Control Center Wuhan 430070China wuhan Animal Disease Prevention and Control Center Wuhan 430016China wuhan Academy of Agricultural Sciences Wuhan 430065China

Porcine adenoviruses(PAdVs)are classified into three species,PAdV-A,PAdV-B,and *** genomes of PAdV-A and PAdV-C have been well ***,the genome of PAdV-B has never been completely sequenced,and the epidemiology of PAdV-B remains *** our study,we have identified a novel strain of PAdV-B,named PAdV-BHNU1,in porcine samples collected in China by viral metagenomic assay and general *** genome of PAdV-BHNU1 is 31,743 bp in length and highly similar to that of California sea lion adenovirus 1(*** lion AdV-1),which contains typical mastadenoviral structures and some unique regions at the carboxy-terminal ***,PAdV-BHNU1 harbors a dUTPase coding region not clustering with other mastadenoviruses except for *** lion AdV-1 and afiber coding region homologous with galectin 4 and 9 of ***,the variance of GC contents between PAdV-BHNU1(55%)and *** lion AdV-1(36%)indicates their differential evolutionary *** epidemiologic study revealed a high positive rate(51.7%)of PAdV-B-HNU1 in porcine lymph samples,but low positive rates of 10.2%and16.1%in oral swabs and rectal swabs,*** conclusion,this study characterized a novel representative genome of a lymphotropic PAdV-B with unique evolutionary origin,which contributes to the taxonomical and pathogenic studies of PAdVs.

关键词： Porcine adenovirus(PAdV) PAdV-B PAdV-B-HNU1 Lymphoid

Effect of proppant sizes and injection modes on proppant transportation and distribution in the tortuous fracture model

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Particuology 2024年第1期84卷 261-280页

作者： jun li Xu Han Siyuan He Mingyi wu Xin Huang Nianyin li State Key Laboratory of Oil and Gas Reservoir Geology and Exploitation Southwest Petroleum UniversityChengdu610500China Chongqing Industry Polytechnic College Chongqing401120China Sichuan Particles Energy and Technology Co. LtdChengdu611630China Engineering Technology Research Institute of PetroChina Southwest Oil and Gas Field Company Chengdu610017China

Particle-fluid transport and placement mechanism in tortuous fracture played a crucial role in uncon-ventional ***,most studies focused on mono-size proppant with fluid transport pro-cesses in tortuous ***,the mixture-size proppant with fluid movement mechanism in tortuous fracture was still ***,this study designed and applied a series of experiments with a physical analog model of a tortuous fracture with 120°and 90°-angled bends and combined high-speed camera-based *** experimental system was used to track different-mixture-sized proppant particle motion trajectories for a series of proppant injection schemes;The following conclu-sions were drawn from this study:*** pile-up processes mechanism in all investigated schemes were similar and could be reduced to four main *** packing structure at both sides of the fracture wall had different variation rates,which were controlled by the mix ratio(change from 1∶1-1∶5)of proppant *** new packing patterns,such as Zebra Stripe,had occurred,controlled by the different proppant injection ***-sized mono-proppant(30/50 mesh)had the highest transport efficiency in the tortuous fracture,followed by the mixed-sized multi-proppant(10/20 mesh:30/50 mesh),large-sized proppant(10/20 mesh)was the *** optimized alternating in-jection mode was recommended as injecting small-sized proppant first(30/50 mesh)and followed by mixed-sized multi-proppant(10/20 mesh:30/50 mesh),which could contribute to obtaining the optimal both proppant packing height and travel distance in tortuous *** correlations were devel-oped for predicting the proppant packing height and transportation distance.

关键词： Tortuous fracture model Mixed-sized proppant Multi-sized proppant injection mode Proppant motion and distribution Packing mechanism

Bimolecular Self-Condensation of Pyrrolizinones Induced byGrignard Reagent

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Chinese Chemical Letters 1999年第11期10卷 893-894页

作者： Wen Ge YAN Shou Fang ZHANG li jun wu(Shenyang Pharmaceutical University, Shenyang 110015) Shenyang Pharmaceutical University Shenyang 110015 China

The products of pyrrolizinones with 3-methoxyphenyl magnesium bromide were bimolecular condensation compounds, not the related alcohol, and they were confirmed by IR, H-1-NMR, C-13-NMR. HMQC, HMBC. NOE-differ., MS and... 详细信息

关键词： pyrrolizinone self-condensation character

Magnetic resonance cholangiopancreatography for the detection of pancreatic duct stones in patients with chronic pancreatitis

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World Journal of Gastroenterology 2009年第20期15卷 2543-2546页

作者： Zhen-Hua Ma Qing-Yong Ma Huan-Chen Sha Sheng-li wu jun Wen Department of Hepatobiliary Surgery the First Affiliated Hospital Medical School of Xi'an Jiaotong University Xi'an 710061 Shaanxi Province China

AIM:To assess the role of magnetic resonance cholangiopancreatography(MRCP) in detection of pancreatic duct stones(PDS) in patients with chronic pancreatitis(CP).METHODS:Clinical data of 78 CP patients who were treated at the First Affi liated Hospital of Xi'an Jiaotong University(China) between January 2004 and July 2008 were retrospectively analyzed.A predictive model of pancreatic duct stones was established through logistic regression and its effectiveness was verifi *** these patients, MRCP was performed in 60 patients who served as a control group, while 44 patients with a higher predictive value than the entry threshold of the predictive model served as an experimental ***:The positive rate of PDS in the 78 patients with CP was 19.2%(15/78).The predictive entry threshold of the predictive model was 5%(P lity of existence of PDS could be predicted according to the following 4 indexes:gastrointestinal symptoms, intermittent abdominal pain, diabetes mellitus(DM)/impaired glucose tolerance(IGT) and positive B-mode ultrasound *** incidence of PDS in the experimental group was higher than that in the control group(P < 0.05).CONCLUSION:MRCP is strongly suggested for the detection of PDS in patients with gastrointestinal symptoms, intermittent abdominal pain, DM/IGT and positive B-mode ultrasound results.

关键词： Chronic pancreatitis Pancreatic duct stone Magnetic resonance cholangiopancreatography B-mode ultrasound Logistic regression

Multi-source data integration and multi-scale modeling framework for progressive prediction of complex geological interfaces in tunneling

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Underground Space 2024年第2期15卷 1-25页

作者： Jingxiao Wang Peinan li Xiaoying Zhuang Xiaojun li Xi Jiang jun wu Department of Geotechnical Engineering College of Civil EngineeringTongji UniversityShanghai 200092China College of Environmental Science and Engineering Donghua UniversityShanghai 201620China Institute of Photonics Department of Mathematics and PhysicsLeibniz University HannoverHannover 30167Germany Department of Civil and Environmental Engineering The Hong Kong Polytechnic UniversityHung HomKowloonHong Kong 100872China College of Civil Engineering Shanghai Normal UniversityShanghai 200233China

A reliable geological model plays a fundamental role in the efficiency and safety of mountain tunnel ***,regional models based on limited survey data represent macroscopic geological environments but not detailed internal geological characteristics,especially at tunnel portals with complex geological *** paper presents a comprehensive methodological framework for refined modeling of the tunnel surrounding rock and subsequent mechanics analysis,with a particular focus on natural space distortion of hard-soft rock interfaces at tunnel *** progressive prediction of geological structures is developed considering multi-source data derived from the tunnel survey and excavation *** improve the accuracy of the models,a novel modeling method is proposed to integrate multi-source and multi-scale data based on data extraction and potential field ***,a regional-scale model and an engineering-scale model are built,providing a clear insight into geological phenomena and supporting numerical *** addition,the proposed framework is applied to a case study,the Long-tou mountain tunnel project in Guangzhou,China,where the dominant rock type is *** results show that the data integration and modeling methods effectively improve model structure *** improved model’s calculation deviation is reduced by about 10%to 20%in the mechanical *** study contributes to revealing the complex geological environment with singular interfaces and promoting the safety and performance of mountain tunneling.

关键词： Mountain tunnel Geological modeling Multi-source data Progressive prediction Tunnel portals