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抗血栓药研究进展

中兽医医药杂志 2024年第4期43卷 32-39页

作者：陶琦冯基范丽萍杨亚军李剑勇中国农业科学院兰州畜牧与兽药研究所农业农村部兽用药物创制重点实验室甘肃省新兽药重点实验室甘肃兰州730050

血栓形成在生理性止血中有着至关重要的作用,能够及时减少血液流失,但病理性血栓将严重危害机体的健康。血小板来源于巨核细胞胞质的分割断裂,其活化后具有多种功能,如黏附、释放和聚集等功能,并参与凝血反应。血栓的形成虽被众多因素影... 详细信息

血栓形成在生理性止血中有着至关重要的作用,能够及时减少血液流失,但病理性血栓将严重危害机体的健康。血小板来源于巨核细胞胞质的分割断裂,其活化后具有多种功能,如黏附、释放和聚集等功能,并参与凝血反应。血栓的形成虽被众多因素影响,但其形成过程基本分为血小板的活化和凝血反应两步,其治疗药物主要分为抗血小板药物和抗凝血药物。目前,多种上市的抗血栓药物经相关受体、信号通路等各种途径抑制血栓的形成,有较好的临床效果,如阿司匹林、华法林等。但近年的研究与应用发现,多种抗血栓药物均存在一定的出血风险。近年来,随着宠物物质生活的不断提高,中老年伴侣动物的心血管疾病发病率呈逐年升高趋势。然而现阶段研究预防血栓形成的药物一般是用于人类,到目前为止,国内还没有宠物专用的预防血栓形成的药物上市。因此,开发新型有效的宠物专用预防血栓药物显得越发重要。本文总结现有抗血栓药的类型和作用机制,以期为宠物专用预防血栓药物的研发提供参考。

关键词：血栓形成血小板凝血因子凝血反应抗血小板药物抗凝血药物

Tyrosine aminotransferase: biochemical and structural properties and molecular dynamics simulations

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Protein & Cell 2010年第11期1卷 1023-1032页

作者： Prajwalini Mehere Qian Han Justin A.Lemkul Christopher J.Vavricka Howard Robinson David R.Bevan jianyong li Department of Biochemistry Virginia TechBlacksburgVA 24061USA CAS Key Laboratory of Pathogenic Microbiology and Immunology Institute of MicrobiologyChinese Academy of SciencesBeijing 100101China Biology Department Brookhaven National LaboratoryUptonNY 11973USA

Tyrosine aminotransferase(TAT)catalyzes the transamination of tyrosine and other aromatic amino *** enzyme is thought to play a role in tyrosinemia type II,hepatitis and hepatic carcinoma *** objective of this study is to investigate its biochemical and structural characteristics and substrate specificity in order to provide insight regarding its involvement in these *** TAT(mTAT)was cloned from a mouse cDNA library,and its recombinant protein was produced using Escherichia coli cells and purified using various chromatographic *** recombinant mTAT is able to catalyze the transamination of tyrosine usingα-ketoglutaric acid as an amino group acceptor at neutral *** enzyme also can use glutamate and phenylalanine as amino group donors and p-hydroxyphenylpyruvate,phenylpyruvate and alpha-ketocaproic acid as amino group *** macromolecular crystallography we have determined the mTAT crystal structure at 2.9Å*** crystal structure revealed the interaction between the pyridoxal-5′-phosphate cofactor and the enzyme,as well as the formation of a disulphide *** detection of disulphide bond provides some rational explanation regarding previously observed TAT inactivation under oxidative conditions and reactivation of the inactive TAT in the presence of a reducing *** dynamics simulations using the crystal structures of Trypanosoma cruzi TAT and human TAT provided further insight regarding the substrate-enzyme interactions and substrate *** biochemical and structural properties of TAT and the binding of its cofactor and the substrate may help in elucidation of the mechanism of TAT inhibition and activation.

关键词： tyrosine aminotransferase crystal structure substrate specificity tyrosine tyrosinemia

Ionospheric total electron content disturbance associated with May 12, 2008,Wenchuan earthquake

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Geodesy and Geodynamics 2015年第2期6卷 126-134页

作者： li jianyong Meng Guojie You Xinzhao Zhang Rui Shi Hongbo Han Yufei National Earthquake Infrastructure Service State Key Laboratory of Geodesy and Earth's Dynamics Institute of Geodesy and GeophysicsChinese Academy of Sciences Institute of Earthquake Science China Earthquake Administration

Possible ionospheric disturbances relating to the May 12, 2008, MsS.0 Wenchuan earthquake were identified by Global Positioning System （GPS）-derived total electron content （TEC）, ion- osonde observations, the global ionospheric map （GIM）, and electron density profiles detected by the Constellation Observation System for Meteorology Ionosphere and Climate （COSMIC）. We applied a statistical test to detect anomalous TEC signals and found that a unique enhancement in TEC, recorded at 16 GPS stations, appeared on May 9, 2008. The critical fre- quency at F2 peak （foF2）, observed by the Chinese ionosondes, and maximal plasma frequency, derived from COSMIC data, revealed a characteristic similar to GPS TEC variations. The GIM showed that the anomalous variations of May 9 were located southeast of the epicenter. Using GPS data from 13 stations near the epicenter, we analyzed the TEC variations of satellite orbit traces during 04：00-11：00 UT. We found that TEC decreased to the east and increased to the southeast of the epicenter during this period. Results showed that the abnormal disturbance on May 9 was probably an ionosphenc precursor of the Wenchuan earthquake of May 12, 2008.

关键词： Ionospheric disturbance Global positioning system (GPS) Total electron content (TEC) Wenchuan earthquake Global ionospheric map (GIM) COSMIC f0F2 Crustal Movement Observation Network of China (CMONOC)

Biochemistry of Melanization in Mosquitoes

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中国农业大学学报 1998年第S2期3卷 181-182页

作者： li jianyong (Dep.of Pathobiology College of Veterinary Medicine,University of Illinois at Urbana-Champaign)

Melanization/pigmentation is an important physiological phenomenon in insects,which is involved incuticular tanning,parasite encapsulation,and egg shell *** the past few years,we have beenusing mosquito eggs as a model to study the detailed biochemistry of melanization reactions in *** of newly oviposited eggs is white and soft,but the chorion becomes black and hard within 3～4 hfollowing oviposition. Once the chorion turns black,the eggs become resistant to desiccation and *** mosquitoes,such as Aedes aegypti,oviposit on substrates at the edge of water and theeggs hatch only after being flooded with water following adequate ***,the ability towithstand the desiccation and other environmental adversities is critical for egg *** research deal-ing with chorion melanization in mosquitoes is aimed at understanding the biochemical processes and mech-

关键词： Biochemistry of Melanization in Mosquitoes

液相色谱-串联质谱法检测猪粪便中除虫脲的方法学研究

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畜牧与兽医 2024年第1期56卷 56-64页

作者：邓润辉范丽萍刘希望秦哲白莉霞葛闻博李世宏李剑勇董书伟杨亚军榆林学院生命科学学院陕西榆林719000 中国农业科学院兰州畜牧与兽药研究所/农业农村部兽用药物创制重点实验室/甘肃省新兽药工程重点实验室甘肃兰州730050

除虫脲(DFB)属于苯甲酰苯脲类化合物,能够抑制昆虫及其幼虫的几丁质合成,从而控制成虫的数量。为了解除虫脲预混剂混饲给药后,猪粪便中药物的浓度,以DFB-^(13)C_(6)为内标,建立了猪粪便中DFB药物浓度的液相色谱-串联质谱(LC-MS/MS)检测... 详细信息

除虫脲(DFB)属于苯甲酰苯脲类化合物,能够抑制昆虫及其幼虫的几丁质合成,从而控制成虫的数量。为了解除虫脲预混剂混饲给药后,猪粪便中药物的浓度,以DFB-^(13)C_(6)为内标,建立了猪粪便中DFB药物浓度的液相色谱-串联质谱(LC-MS/MS)检测方法。以冰乙酸-乙腈提取粪便中的DFB,采用QuEChERS技术净化提取液,LC-MS/MS测定,内标法定量。结果显示,猪粪便样品基质对于DFB的定量检测干扰较小,检测方法灵敏度高,检测限为10 ng/g,定量限为20 ng/g;检测方法的基质效应对样品的检测影响较小;标准溶液在2~500 ng/mL的浓度范围内,线性关系良好(R^(2)≥0.99);在定量限20 ng/g及低浓度(100 ng/g)、中浓度(1000 ng/g)、高浓度(5000 ng/g)的添加水平下,平均准确度在93%~110%,批内、批间变异系数均小于10%,符合方法学要求;不同浓度加标样品处理后的溶液,室温放置24 h稳定性良好,但应避免样品的反复冻融。结果表明,本试验建立的LC-MS/MS检测方法可用于猪粪便中DFB药物浓度的检测,为后期开展除虫脲预混剂的临床试验提供了可靠方法。

关键词：猪粪便除虫脲液相色谱-串联质谱法内标法

维普期刊数据库博看期刊

Polymorphisms in XRCC5,XRCC6,XRCC7 genes are involved inDNA double-strand breaks(DSBs) repair associated with the risk ofacute myeloid leukemia(AML) in Chinese population

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Journal of Nanjing Medical University 2009年第2期23卷 93-99页

作者： Guoqiang Wang Shuyu Wang Qun Shen Shiwei Yin Chunping li Aiping li jianyong li Jianwei Zhou Qizhan liu Department of Health Toxicology School of Public Health Nanjing Medical University Nanjing 210029 China Department of Hematology Jiangsu Province Hospital of Traditional Chinese Medicine Nanjing 210029 China Department of Hematology the First Affiliated Hospital of Nanjing Medical University Nanjing 210029 China

Objective：To investigate the association between the X-ray repair cross complementing（XRCC） group 5, XRCC6 and XRCC7 polymorphisms and risk of acute myeloid leukemia（AML）. Methods：This hospital-based case-control study included 120 AML patients and 210 cancer-free controls in a Chinese population. Three polymorphisms of XRCC5, XRCC6 and XRCC7 were genotyped using the polymerase chain reaction（PCR） or polymerase chain reaction-restriction fragment length polymorphism（PCR-RFLP） method. Results： We found that there was a significant decrease in risk of AML associated with the XRCC6 -61 CG/GG genotype（adjusted odd ratio （OR） = 0.55; 95% confident interval（CI） = 0.34-0.89） compared with the -61CC genotype. For the novel tandem repeat polymorphism （VNTR） in the XRCC5 promoter, we found when the XRCC5 six genotypes were dichotomized（i.e., 2R/2R, 2R/1R versus 2R/0R, 1R/1R, 1R/0R and 0R/0R）, the latter group was associated with increased risk of AML（adjusted OR = 1.67; 95% CI = 1.00-2.79） compared to 2R/ 2R＋2R/1R genotype. However, the XRCC7 6721G〉T polymorphism had no effect on risk of AML. Conclusion：The XRCC6 -61C 〉 G and XRCC5 2R/1R/0R polymorphisms, but not XRCC7 6721G 〉 T polymorphism, could play an important role in the development of AML. Larger scale studies with more detailed data on environment exposure are needed to verify these findings.

关键词： XRCC5 XRCC6 XRCC7 single nucleotide polymorphism tandem repeat polymorphism acute myeloid leukemia

The combination therapy of imatinib and dasatinib achieves long-term molecular response in two imatinib-resistant and dasatinibintolerant patients with advanced chronic myeloid leukemia

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The Journal of Biomedical Research 2016年第6期30卷 525-528页

作者： Yu Zhu liangqin Pan Ming Hong Weixing liu Chun Qiao jianyong li Sixuan Qian Department of Hematology the First Affiliated Hospital of Nanjing Medical University

For patients with chronic myeloid leukemia（CML） failing imatinib therapy,second-generation tyrosine kinase inhibitors（TKIs） are ***,we describe two patients with advanced CML who failed imatinib therapy and did not tolerate the recommended dose of dasatinib,but then achieved a major molecular response with the combination of imatinib and dasatinib with no significant extramedullar/ *** observations suggest that combination of TKIs may provide an additive/synergistic antileukemic effect.

关键词： chronic myeloid leukemia tyrosine kinase inhibitors resistance combined therapy

Extended light absorption and accelerated charge migration in ultrathin twisted carbon nitride nanoplates for efficient solar hydrogen production

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Science China Chemistry 2024年第7期67卷 2215-2223页

作者： Yanmin Zou Shiyao li Dandan Zheng jianyong Feng Sibo Wang Yidong Hou Guigang Zhang State Key Laboratory of Photocatalysis on Energy and Environment College of ChemistryFuzhou UniversityFuzhou 350108China College of Environment and Safety Engineering Fuzhou UniversityFuzhou 350108China Collaborative Innovation Center of Advanced Microstructures National Laboratory of Solid State MicrostructuresCollege of Engineering and Applied SciencesNanjing UniversityNanjing 210093China

Polymeric carbon nitride has been widely developed as a promising photocatalyst for solar hydrogen production via photocatalytic water ***,pristine carbon nitride prepared by traditional solid-state polymerization usually encounters issues such as rapid carrier recombination and insufficient absorption of visible light below 460 ***,poly(heptazine imide)with a distinctive nanoplate structure was synthesized in a binary molten salt of NaCl–CaCl_(2).The salt template allows the formation of the thin nanoplate structure,which promotes the charge separation and ***,the intercalation of Ca^(2+)ions between the conjugated layers endows the activation of n–π*electron transition due to the distortion of in-plane heptazine ***,the optimized poly(heptazine imide)nanoplates achieve an apparent quantum efficiency of up to 17.3%at 500 nm for photocatalytic hydrogen production from *** work shares new idea for rational control of the optical absorption and charge carrier dynamics of poly(heptazine imide).

关键词： photocatalysis poly heptazine imide nanoplates ion intercalation hydrogen production

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蔬菜中硝酸盐、亚硝酸盐残留状况及调控对策

上海农业学报 2024年第2期40卷 89-93页

作者：占绣萍李建勇方朝阳王齐旭张恒铖孔令娟上海市农业技术推广服务中心上海201103 上海市松江区新浜镇农业农村服务中心上海201605

硝酸盐和亚硝酸盐残留量是评价蔬菜等农产品安全的一个重要指标。在查阅国内外文献资料基础上,结合上海市蔬菜产品中硝酸盐、亚硝酸盐含量的检测结果,对蔬菜中硝酸盐和亚硝酸盐的危害、残留状况进行了梳理分析。对有机蔬菜和常规种植蔬... 详细信息

硝酸盐和亚硝酸盐残留量是评价蔬菜等农产品安全的一个重要指标。在查阅国内外文献资料基础上,结合上海市蔬菜产品中硝酸盐、亚硝酸盐含量的检测结果,对蔬菜中硝酸盐和亚硝酸盐的危害、残留状况进行了梳理分析。对有机蔬菜和常规种植蔬菜、不同种类蔬菜、同一种类蔬菜不同品种、不同收获时间之间硝酸盐(亚硝酸盐)的残留差异与可能的原因进行了探讨,提出了科学施用氮肥等调控对策,并呼吁科研工作者加快开展蔬菜中硝酸盐(亚硝酸盐)生态减控综合技术集成研究及田间生产应用。

关键词：蔬菜硝酸盐亚硝酸盐残留调控