检索结果-南通市图书馆

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

淋巴造血组织病理学的十年回顾和展望

中华病理学杂志 2005年第8期34卷 499-501页

作者：李甘地四川大学华西医院病理科

在庆祝创刊50周年之际,我受杂志编辑部的委托,对我国大陆1995年至2004年10年来淋巴造血组织病理学的发展进行回顾和展望。

关键词：组织病理学造血淋巴《中华病理学杂志》留学回国人员学术水平 2004年 1995年 50周年

Features of intestinal T-cell lymphomas in Chinese population without evidence of celiac disease and their close association with Epstein-Barr virus infection

在线全文

学校读者我要写书评

暂无评论

Chinese Medical Journal 2005年第18期118卷 1542-1548页

作者： ZHANG Wen-yan li gan-di liU Wei-ping OUYANG Qin REN Xing-chang li Feng-yuan XU Huan Department of Pathology West China Hospital Sichuan UniversityChengdu 610041 China Department of Gastroenterology West China Hospital SichuanUniversity Chengdu 610041 China

Background Intestinal T-cell lymphoma （ITCL） is a heterogeneous lymphoid neoplastic group with variable clinical and pathological features. ITCL in oriental countries is different from enteropathy-type intestinal T-cell lymphoma （ETCL） in relation to celiac disease and Epstein-Barr virus （EBV）. The objective of this study was to investigate the clinicopathological features, immunophenotype, expression of cytotoxic molecule （TIA-1）, T-cell receptor （TCR）-γ gene rearrangement, and Epstein-Barr virus （EBV） latent infection in primary ITCL without celiac disease in Chinese. Methods The clinical data of 42 patients were analyzed, and the patients were followed up. Compared with human reactive lymphoid tissues, in situ hybridization for EBER1/2, polymerase chain reaction for TCR-~/gene rearrangement, and immunohistochemical staining for immunophenotypes, TIA-1 and EBV latent membrane proteins （LMP-1） were investigated. Survival curves of different clinicopathological features, immunophenotypes, expression of LMPI , TCR-γ/gene rearrangement and therapy were analyzed. Results Three fourths of the patients suffered from ITCL in China were men with a peak age incidence in the 4th decade. Common presenting features included fever and hemotochezia. The prognosis was poor with a median survival of 3.0 months. The lesions were mostly localized in the ileocecum and colon. About 38/42 （90. 5% ） patients demonstrated pleomorphic medium-sized on large seen. All 42 patients with ITCL revealed CD45RO cells. Histological features of celiac disease were rarely positive. Neoplastic cells partially expressed T-cell differentiated antigens （CD3ε, CD4, CD8） and NK cell associated antigen （CD56）. The positive frequency of CD3e, CIM, CD8 and CD56 was 28/42 （66.7%） 7/42 （16.7%）, 10/42 （23.8%） and 12/42 （28.6%） respectively. Thirty-nine cells （92. 9% ） expressed TIA-1, but none expressed CD20 and CD68. More than half of the patients （64. 3% , 64.3% and 59.5% ） revealed TCR-γ primers respectively

关键词： intestinal T-cell lymphoma·pathology·immunophenotype·gene rearrangement gamma-chain T-cell antigen receptor·Epstein-Barr virus

在线全文

学校读者我要写书评

暂无评论

儿童常见淋巴造血系统肿瘤的诊断和鉴别诊断

中华病理学杂志 2005年第12期34卷 765-768页

作者：李甘地四川大学华西医院病理科成都610041

儿童的多数恶性肿瘤,包括实体瘤与非实体瘤,在病理学上大多表现为一致性小细胞(母细胞)组成的肉瘤,以往称为"小蓝细胞肉瘤",其病理诊断有时十分困难.近年来由于免疫组织化学、流式细胞术和基因重排、染色体易位检测技术的发展,儿童的小... 详细信息

儿童的多数恶性肿瘤,包括实体瘤与非实体瘤,在病理学上大多表现为一致性小细胞(母细胞)组成的肉瘤,以往称为"小蓝细胞肉瘤",其病理诊断有时十分困难.近年来由于免疫组织化学、流式细胞术和基因重排、染色体易位检测技术的发展,儿童的小细胞肉瘤的诊断与鉴别诊断有了巨大的进展,同时带来治疗上的进步.目前多数儿童的淋巴瘤/白血病是可以治愈的.可以说儿童淋巴瘤/白血病,尤其是实体瘤的诊断与治疗领先于成人肿瘤。

关键词：造血系统肿瘤鉴别诊断淋巴瘤儿童免疫组织化学流式细胞术染色体易位实体瘤恶性肿瘤病理诊断

Lymphomatoid papulosis type D in a Chinese young man： a newly recognized variant

在线全文

学校读者我要写书评

暂无评论

Chinese Medical Journal 2013年第20期126卷 3997-3998页

作者： Wen Peng-fei Jia ling Zhang Min li gan-di liu Wei-ping Wang lin Sichuan Univ West China Hosp Dept Dermatol Chengdu 610041 Sichuan Peoples R China Sichuan Univ West China Hosp Dept Pathol Chengdu 610041 Sichuan Peoples R China Chengdu Second Peoples Hosp Dept Dermatol Chengdu 610017 Sichuan Peoples R China

To the editor： We report a 23-year-old Chinese man, who presented with a 2-month history of recurrent eruptions of asymptomatic, red, necrotic papules on his face and extremities. The skin lesions would regress spontaneously within several days, leaving atrophic scars. He had no systemic symptoms. A physical examination revealed many papules and nodules on his trunk and extremities （Figure I A）. Some papules had a necrotic center covered by black crusts. No lymphadenopathy was found.

关键词：

Clinicopathology, immunophenotype, T cell receptor gene rearrangement, Epstein-Barr virus status andp53 gene mutation of cutaneous extranodal NK/T-cell lymphoma, nasal-type

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Medical Journal 2013年第7期126卷 1281-1287页

作者： WANG Ting-ting XU Chen liU Shan-ling KAN Bei RAN Yu-ping liU Wei-ping li gan-di WANG lin Department of Dermatovenereology West China Hospital Sichuan University Chengdu Sichuan 610041 China Department of Pathology West China Hospital Sichuan University Chengdu Sichuan 610041 China

Background Extranodal natural killer/T-cell （NK/T cell） lymphoma, nasal-type, is a rare lymphoma. Skin is the second most common site of involvement after the nasal cavity/nasalpharynx. The aim of this study was to investigate the clinicopathologic features, immunophenotype, T cell receptor （TCR） gene rearrangement, the association with Epstein-Barr virus （EBV） infection and p53 gene mutations of the lymphoma. Methods The clinicopathologic analysis, immunohistochemistry, in situ hybridization for EBERI/2, TCR gene rearrangement by polymerase chain reaction （PCR）, mutations of p53 gene analyzed by PCR and sequence analysis were employed in this study. Results In the 19 cases, the tumor primarily involved the dermis and subcutaneous layer. Immunohistochemical staining showed that most of the cases expressed CD45RO, CD56, CD3E, TIA-1 and GrB. Three cases were positive for CD3 and two cases were positive for CD30. Monoclonal TCRy gene rearrangement was found in 7 of 18 cases. The positive rate of EBERI/2 was 100%. No p53 gene mutation was detected on the exon 4-9 in the 18 cases. Fifteen cases showed Pro （proline）/Arg （arginine） single nucleotide polymorphisms （SNPs） on the exon 4 at codon 72. The expression of p53 protein was 72% （13/18）immunohistochemically. Conclusions Cutaneous NK/T-cell lymphoma is a rare but highly aggressive lymphoma with poor prognosis. No p53 gene mutation was detected on the exon 4-9, and Pro/Arg SNPs on p53 codon 72 were detected in the cutaneous NK/T-cell lymphoma. The overexpression of p53 protein may not be the result of p53 gene mutation.

关键词： NK/T-cell cutaneous lymphoma p53 gene mutations Epstein-Barr virus

Recent Strategies and Advances in Hydrogel‑Based Delivery Platforms for Bone Regeneration

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Nano-Micro Letters 2025年第3期17卷 389-439页

作者： Xiao Wang Jia Zeng Donglin gan Kun ling Mingfang He Jianshu li Yongping Lu Scientific and Technological Innovation Center for Biomedical Materials and Clinical Research Guangyuan Key Laboratory of Multifunctional Medical HydrogelGuangyuan Central HospitalGuangyuan 628000People’s Republic of China Jiangsu Collaborative Innovation Center of Biomedical Functional Materials Jiangsu Key Laboratory of Bio‑Functional MaterialsSchool of Chemistry and Materials ScienceNanjing Normal UniversityNanjing 210023People’s Republic of China College of Polymer Science and Engineering State Key Laboratory of Polymer Materials EngineeringSichuan UniversityChengdu 610065People’s Republic of China

Bioactive molecules have shown great promise for effectively regulating various bone formation processes,rendering them attractive therapeutics for bone ***,the widespread application of bioactive molecules is limited by their low accumulation and short half-lives in *** have emerged as ideal carriers to address these challenges,offering the potential to prolong retention times at lesion sites,extend half-lives in vivo and mitigate side effects,avoid burst release,and promote adsorption under physiological *** review systematically summarizes the recent advances in the development of bioactive molecule-loaded hydrogels for bone regeneration,encompassing applications in cranial defect repair,femoral defect repair,periodontal bone regeneration,and bone regeneration with underlying ***,this review discusses the current strategies aimed at improving the release profiles of bioactive molecules through stimuli-responsive delivery,carrier-assisted delivery,and sequential ***,this review elucidates the existing challenges and future directions of hydrogel encapsulated bioactive molecules in the field of bone regeneration.

关键词： Hydrogel Bone regeneration Bioactive molecules Drug delivery Nano-/microscale carriers

石蜡包埋组织快速DNA提取方法在诊断病理PCR分析中的应用研究

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

四川大学学报（医学版） 2007年第4期38卷 709-712页

作者：江炜刘卫平李雷李甘地四川大学华西医院病理科成都610041

目的改良石蜡组织DNA快速提取方法的流程,评价其提取效率,探讨其应用于病理诊断PCR分析的可行性。方法选取包括淋巴瘤及反应性病变的52例存档蜡块,以缓冲液煮沸方法快速提取DNA,并以传统酚-氯仿抽提法和试剂盒提取法作为对照,采用PCR反... 详细信息

目的改良石蜡组织DNA快速提取方法的流程,评价其提取效率,探讨其应用于病理诊断PCR分析的可行性。方法选取包括淋巴瘤及反应性病变的52例存档蜡块,以缓冲液煮沸方法快速提取DNA,并以传统酚-氯仿抽提法和试剂盒提取法作为对照,采用PCR反应扩增看家基因β-Globin以确定模板DNA质量,并通过测序确定结果的可靠性;同时根据临床病理诊断选择性的进行免疫球蛋白重链基因(IgH)或T细胞受体γ基因(TCRγ)重排检测。结果快速法提取DNA的浓度虽低于对照方法,但β-Globin扩增效率与对照方法相比差异没有统计学意义(P>0.05),对IgH和TCRγ重排的检出率与对照相比差异亦没有统计学意义(P均>0.05)。测序结果显示扩增片段与目的基因序列完全符合。DNA可在-20℃保存至少4周。结论快速DNA提取法可以从存档的石蜡包埋组织中提供足量的PCR反应模板,结果稳定可靠,且省时省力,在病理诊断PCR分析中具有应用价值。

关键词：石蜡包埋 DNA提取 PCR 淋巴瘤

浆膜腔积液中的淋巴造血组织肿瘤细胞病理诊断分析

在线全文

学校读者我要写书评

暂无评论

中华病理学杂志 2009年第8期38卷 542-546页

作者：苏学英许霞唐源李甘地四川大学华西医院病理科成都610041

目的探讨用细胞病理方法诊断浆膜腔积液中的淋巴造血组织肿瘤的可行性和准确性，避免漏诊和误诊，评估用细胞块切片进行免疫细胞化学染色的作用。方法收集通过组织活检证实的伴发浆膜腔积液的淋巴造血组织肿瘤33例，对其临床特点、细胞... 详细信息

目的探讨用细胞病理方法诊断浆膜腔积液中的淋巴造血组织肿瘤的可行性和准确性，避免漏诊和误诊，评估用细胞块切片进行免疫细胞化学染色的作用。方法收集通过组织活检证实的伴发浆膜腔积液的淋巴造血组织肿瘤33例，对其临床特点、细胞形态、免疫表型特征等进行分析，另外1例结外鼻型NK／T细胞淋巴瘤进行EBER原位杂交检测，3例T淋巴母细胞白血病／淋巴瘤、2例弥漫性大B细胞淋巴瘤（DLBCL）和2例伯基特淋巴瘤进行了基因重排分析。结果累及浆膜腔的33例淋巴造血组织肿瘤包括T淋巴母细胞白血病／淋巴瘤12例；成熟B细胞肿瘤16例，其中DLBCL9例，伯基特淋巴瘤2例，浆细胞骨髓瘤2例，慢性淋巴细胞白血病／小B细胞淋巴瘤2例，套细胞淋巴瘤1例；成熟T细胞和NK细胞肿瘤3例，其中血管免疫母细胞性T细胞淋巴瘤、结外鼻型NK／T细胞淋巴瘤、T细胞幼淋巴细胞性白血病各1例；粒细胞肉瘤和肥大细胞肉瘤各1例。8例DLBCL、2例浆细胞骨髓瘤、慢性淋巴细胞白血病／小B细胞淋巴瘤、1例套细胞淋巴瘤、T淋巴母细胞白血病／淋巴瘤、血管免疫母细胞性T细胞淋巴瘤、肥大细胞肉瘤共16例为复发病例，其余17例均以浆膜腔积液为首发表现并由细胞病理初诊。所有病例细胞病理学诊断均与组织病理学诊断结果相符。结论结合临床特点、细胞形态、免疫表型、原位杂交和基因重排等检测，用细胞病理方法可以对浆膜腔积液中的淋巴造血组织肿瘤，特别是复发病例进行明确诊断和鉴别诊断。

关键词：血液肿瘤细胞学技术病理学免疫组织化学诊断,鉴别

在线全文

学校读者我要写书评

暂无评论

淋巴母细胞淋巴瘤及其分子遗传学研究进展

中华病理学杂志 2005年第4期34卷 236-239页

作者：潘云李甘地刘卫平四川大学华西医院病理科成都610041

淋巴母细胞淋巴瘤(lymphoblastic lymphoma,LBL)是一类少见的,来源于不成熟前体淋巴细胞的高侵袭性肿瘤,约占所有非霍奇金淋巴瘤(Non-Hodgkin Lymphomas,NHL)的2%～8.5%[1,2],被列入Rappaport 分类的分化不良淋巴细胞淋巴瘤,Lukes 和Col... 详细信息

淋巴母细胞淋巴瘤(lymphoblastic lymphoma,LBL)是一类少见的,来源于不成熟前体淋巴细胞的高侵袭性肿瘤,约占所有非霍奇金淋巴瘤(Non-Hodgkin Lymphomas,NHL)的2%～8.5%[1,2],被列入Rappaport 分类的分化不良淋巴细胞淋巴瘤,Lukes 和Collins 分类的曲形核T细胞淋巴瘤,Sternberg肉瘤.随着对淋巴瘤以及急性白血病的进一步认识,发现LBL和急性淋巴母细胞白血病(acute lymphoblastic leukemia,ALL)是一组临床和实验室特征共同界定的同一肿瘤实体[3],这些特征包括细胞形态学、免疫表型、基因型、细胞遗传学以及临床表现和预后,因此2001年版WHO分类[4],以REAL分型为参考将LBL和ALL均列入前体B/T细胞中,命名为前体B/T淋巴母细胞白血病/淋巴瘤或前体B/T细胞急性淋巴母细胞白血病.当骨髓中肿瘤性淋巴母细胞数超过25%为白血病;而少于25%为淋巴瘤.LBL/ALL根据细胞起源不同主要分为T、B细胞,LBL 表达T细胞表型的占大多数,约为80%以上,不到20%表达B细胞或其他罕见表型.本文从临床特点、病理形态学、免疫表型、基因型和分子遗传学等方面分别对T、B-LBL/ALL的研究进展作一综述。

关键词：淋巴母细胞淋巴瘤 LBL 分子遗传学淋巴细胞肿瘤