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Deciphering the role of mtH2O2 in hepatic ischemia-reperfusion injury mechanisms with low-background super-resolution fluorogenic probe

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Science China Chemistry 2025年第01期 297-307页

作者： Bin Fang limin wang Haoqin li Jiaxin Zhang Yang Ding Pan Pan li Bo Peng hua Bai lin li Frontiers Science Center for Flexible Electronics Xi'an Institute of Flexible Electronics (IFE) and Xi'an Institute of Biomedical Materials &Engineering Northwestern Polytechnical University Institute of Flexible Electronics (IFE Future Technologies) Xiamen University

Hepatic ischemia-reperfusion injury（HIRI） is characterized by mitochondrial dysfunction and oxidative stress. Monitoring mitochondrial hydrogen peroxide（mtH2O2） levels in real-time through super-resolution imaging is crucial for elucidating its distribution in live cells and its mechanism of action during HIRI. However, low-background fluorogenic probes have been overlooked in the context of super-resolution imaging. In this study, we developed a low-background fluorogenic probe（MitoWG） with the potential for super-resolution morphology-correlated mitochondrial identification to track the fluctuates of mtH2O2in HIRI. Activation of the desirable fluorescence properties of the probe by mtH2O2was confirmed using structural illumination microscopy（SIM）, enabling high-quality mitochondrial imaging with exceptional specificity and *** in mtH2O2levels were successfully observed in both cellular and rat models of HIRI. Furthermore, we associated the decline in mitochondrial redox homeostasis with accelerated mtH2O2production during HIRI, which triggered mitophagy deficiency and led to cell death. In conclusion, Mito-WG possesses excellent photophysical and low-background properties for SIM imaging, making it a promising tool for mtH2O2tracking in HIRI research and clinical diagnosis.

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Neutral sulfate berberine modulates cytokine secretion and increases survival in endotoxemic mice

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Acta Pharmacologica Sinica 2006年第9期27卷 1199-1205页

作者： Fei li hua-dong wang~2 Da-xiang LU Yan-ping wang Ren-bin QI Yong-mei FU Chu-jie li Department of Pathophysiology,Medical College of Jinan University,Guangzhou 510632,China DepartmentofPathophysiology MedicalCollegeofJinanUniversityGuangzhou510632China

Aim:Berberine is thought to be an immunomodulator,so the present study aimedto investigate the effect of berberine on mortality,lung and intestine injury inendotoxemic mice,and the mechanism of its ***:Mice were chal-lenged with lipopolysaccharide(LPS,28 mg/kg,ip),and neutral sulfate berbefinewas administrated *** was monitored every 12 h,and his-tology of the lungs and intestine as well as the plasma tumor necrosis factor-α(TNF-α),interferon-γ(IFN-γ),interleukin-12(IL-12),IL-10,and nitric oxide(NO)levels were ***:Pretreatment with 50 mg/kg neutral sulfate ber-berine once a day for 5 days significantly decreased the mortality rate and attenu-ated tissue injury of the lungs and small intestine in mice challenged with *** stimulated a marked increase in plasma levels of TNF-α,IFN-γ,IL-12,IL-10,and *** administration of berberine significantly reduced plasma TNF-α,IFN-γ,and NO levels,but did not suppress plasma IL-12 levels in mice exposed ***,pretreatment with neutral sulfate berbefine augmented IL-10secretion stimulated by LPS in ***:Pretreatment with neutral sul-fate berberine attenuates tissue injury and improves survival in endotoxemic mice,which may be mediated,at least in part,by the inhibition of pro-inflammatorymediator production and upregulation of IL-10 *** findings mightprovide a new strategy for the treatment of endotoxemia.

关键词： lipopolysaccharide berberine tumor necrosis factor-α interleukin-10 interleukin-12 interferon-γ nitric oxide mice

Prophylaxis of hepatitis B recurrence in post-liver transplantation patients with lamivudine-resistant YMDD mutant

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Chinese Medical Journal 2007年第16期120卷 1400-1403页

作者： ZHANG Qi CAI Chang-jie LU Ming-qiang li Xi JIANG Nan JIANG hua XU Chi li hua wang Gen-shu YI Shu-hong ZHANG Jian ZHANG Jun-feng YI Hui-min ZHANG Ying-cai CHEN Gui-hua liver Transplant Center Third Affiliated Hospital of Sun Yat-sen University Transplantation Research Institute of Sun Yat-sen University Guangzhou 510630 China

Background The most frequently used therapy for post-transplantation recurrence of hepatitis B virus （HBV） infection is lamivudine, but this drug is associated with a high resistance rate due to YMDD mutant. In preliminary reports, adefovir dipivoxil （ADV） has been shown to have activity against lamivudine-resistant strains of HBV. However, clinical experience in treatment of HBV infection after liver transplantation （LT） is still not entirely clear. This study was aimed to evaluate the prophylactic efficacy of ADV plus hepatitis B immunoglobulin （HBIG） in patients with YMDD mutant before LT. Methods From March 2004 to March 2006, 16 patients with chronic hepatitis B had lamivudine-resistant YMDD mutants detected prior to liver transplantation and received treatment with ADV plus additional intramuscular HBIG after LT as prophylaxis against graft reinfection. Tests for liver function, serum HBsAg, anti-HBs （HBIG）, HBeAg, anti-HBc, anti-HBe, HBV-DNA, and creatinine were assessed pre- or post-liver transplantation. Results The median follow-up of these patients post-liver transplantation was 19.4 months. Fifteen patients survived and one patient died of recurrence of hepatocellular carcinoma （HCC）. There was significant difference （10.98% vs. 2.26%, P〈0.05） in YMDD mutant rate between the patients with HBV-DNA over 106 copies/ml and those with HBV-DNA less than 106 copies/mi. Fifteen patients （93.8%） had undetectable HBV-DNA at 4 weeks and 1 （6.3%） at 6 months after LT. No hepatitis B recurrence was detected by persistent testing of HBsAg, HBeAg, and HBV-DNA and no increase of serum creatinine level associated with ADV was observed in any of the patients. Conclusion ADV combined with intramuscular HBIG can effectively prevent patients with pre-transplantation YMDD mutant from HBV recurrence after LT.

关键词： liver transplantation adefovir dipivoxil YMDD mutant HBV recurrence

Early liver retransplantation versus late liver retransplantation： analysis of a single-center experience

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Chinese Medical Journal 2008年第20期121卷 1992-1996页

作者： CHEN Gui-hua FU Bin-sheng YANG Yang CAI Chang-jie LU Min-qiang li hua wang Gen-shu YI Shu-hong XU Chi ZHANG Jun-feng ZHANG Tong wang Guo-ying liver Transplantation Center Third Affiliated Hospital of Sun Yat-sen University Transplantation Research Institute of Sun Yat-sen University Guangzhou Guangdong 510630 China

Background Orthotopic liver retransplantation （re-OLT） is the only effective therapy for irreversible failure of a liver graft. Early and late graft failure gives way to two different clinical conditions that should be discussed separately. This study was designed to compare early and late re-OLT for patients with poor graft function after primary transplantation at our center and sum up our clinical experience in re-OLT. Methods The clinical data of 31 re-OLTs at our center from January 2004 to February 2007 were analyzed retrospectively, consisting of the first group with 14 cases of early re-OLT and the second group with 17 cases of late re-OLT.Results Biliary tract complications were the main indications for early re-OLT （57.1%） and late re-OLT （52.9%）. Other common indications were vascular complications in early re-OLT and recurrence of primary diseases in late re-OLT. No significant differences were found between the groups with regard to the volume of bleeding during operation, cold ischemia time, operative duration, and perioperative mortality; except for the model of end-stage liver disease （MELD） score. Outcome was fatal for 7 patients in early re-OLT and 9 patients in late re-OLT. Two deaths were due to multiple organ failure with 3 deaths due to severe sepsis-related disease in early re-OLT, and 4 deaths were due to severe sepsis-related disease with 3 deaths due to recurrence of hepatocellular carcinoma （HCC） in late re-OLT. One and 2-year actuarial survival rates after re-OLT were 55.2% and 36.9%, respectively, for patients in early re-OLT, and 65.1% and 52% respectively, for patients in late re-OLT. No significant differences were found regarding survival rates between the two groups. Conclusions Similar clinical results can be achieved in early and late re-OLT. Proper indications and optimal operation timing, adequate preoperative preparation, experienced surgical procedures, and effective perioperative anti-infection strategy contribute to th

关键词： liver transplantation retransplantation survival rate

Expression of cysteinyl leukotriene receptor 2 and aquaporine-4 in edematous human brain tumors

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Expression of cysteinyl leukotriene receptor 2 and aquaporin...

DNA辅助纳米金三角片的高效纯化与信息编码

中华医学会神经外科学分会第九次学术会议

作者： Feng Yan,Gao Chen,Qun li,lin wang,hua Hu 浙江大学医学院附属第二医院神经外科

Background Brain edema is brain swelling that occurs due to the accumulation of excess water in the brain parenchyma. Vasogenic edema,of which brain tumour edema is prime example,is produced by water flow into the ext... 详细信息

关键词： Cysteinyl leukotriene receptor 2 Aquaporin 4 Blood-brain barrier Glioma Peritumoral edema

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高分子学报 2019年第9期50卷 964-972,I0004页

作者：鲁爽方维娜王丽华柳华杰中国科学院上海应用物理研究所上海201800 中国科学院大学北京100049 上海交通大学医学院附属仁济医院分子医学研究院上海200127 同济大学化学科学与工程学院上海200092

针对目前纳米金三角片的分离纯化手段仍不够成熟这一问题,提出借助巯基修饰的DNA分子,对混有类球形颗粒等杂质的纳米金三角片粗产物,进行形状选择性分离纯化的新思路.纳米金表面修饰的DNA分子,一方面屏蔽了金与溶液环境的直接接触,提高... 详细信息

针对目前纳米金三角片的分离纯化手段仍不够成熟这一问题,提出借助巯基修饰的DNA分子,对混有类球形颗粒等杂质的纳米金三角片粗产物,进行形状选择性分离纯化的新思路.纳米金表面修饰的DNA分子,一方面屏蔽了金与溶液环境的直接接触,提高了颗粒的稳定性;另一方面,形貌粒径以及表面DNA修饰量的差异,造成不同纳米金颗粒在电场中的迁移速度不同,因而可通过凝胶电泳手段分离纯化金颗粒.该方法获得的纳米金三角片纯度在80%以上,并对于不同粒径的三角片纯化具有普适性.更为重要的是,表面修饰的DNA同时实现了对纳米金三角片的信息编码,通过设计修饰DNA序列信息,可以实现后续的可控组装,构建更高级结构.

关键词：纳米金颗粒金三角片 DNA 自组装电泳纯化

Using Modified Self-adaptive Differential Evolution for Estimation of Chemical Reaction Kinetic Parameters

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Using Modified Self-adaptive Differential Evolution for Esti...

作者： li-yu-xin CHEN Dong-xiang ZHANG li-hua wang Jian-feng SU School of Chemistry and Chemical Engineering Beijing Institute of Technology

In order to accurately estimate the reaction kinetic parameters, a novel modified self-adaptive differential evolution（MSaDE） algorithm is proposed in this paper, aiming at the problems of premature or local optimization of differential evolution（DE）. MSaDE advances a mutation strategy candidate pool, a given interval of mutation factor（F） and crossover factor（CR）. In the evolutionary process, mutation strategy, F and CR of the next generation are self-adaptively adjusted according to the previous state information of search process. Through five commonly used benchmark functions, it shows that MSaDE can effectively improve the global optimal searching capability, with higher search accuracy and faster convergence rate. In addition, MSaDE is applied to estimate the kinetic parameters of ammonium perchlorate（AP） thermal decomposition reaction models, satisfactory results are obtained.

关键词： Differential evolution algorithm Self-adaptive Parameter estimation

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应用聚类和粗糙集的FPGA底层数据挖掘

光学精密工程 2013年第1期21卷 233-238页

作者：姜大力赵波王永李华旺杨根庆中国科学院微系统与信息技术研究所上海200050 中国科学院大学北京100039 中国科学院长春光学精密机械与物理研究所吉林长春130022

提出了一种从XDL文件中提取现场可编程门阵列(FPGA)底层逻辑和布线资源的方法。预处理阶段通过正则表达式将原有底层逻辑文件转换为待分析的有层次属性的关系型数据库。数据挖掘阶段则根据各个层次数据内部的特性不同,采用不同的算法进... 详细信息

提出了一种从XDL文件中提取现场可编程门阵列(FPGA)底层逻辑和布线资源的方法。预处理阶段通过正则表达式将原有底层逻辑文件转换为待分析的有层次属性的关系型数据库。数据挖掘阶段则根据各个层次数据内部的特性不同,采用不同的算法进行聚类来得到初步知识。通过粗糙集分析初步知识间的关系和约简属性,得出初步知识间的联系同时进一步提取出决策规则和产生式规则的知识。最后,通过规则验证器和泛化器对提取出的规则进行验证和泛化。实验结果表明,对于大型的FPGA器件,wire的逻辑最高压缩比可以达到2.88×10-4。该方法相对于底层器件有较好的通用性和交互性,适用于对不同器件族FPGA底层信息的知识提取,对深入研究FPGA的拓扑架构,提高对FPGA进行动态重配置的可控性和实现更灵活的重配置很有意义。

关键词：现场可编程门阵列数据挖掘粗糙集聚类

Coil migrating out of a small wide-necked aneurysm embolized with stent-assisted technique

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介入放射学杂志 2006年第2期15卷 126-128页

作者： GAO Bu-lang li Ming-hua wang Yong-li FANG Chun Department of Radiology Shanghai Sixth People＇s HospitalShanghai Jiaotong UniversityShanghai 200233Chian

Stent-assisted coiling is one of the techniques used to treat intracranial aneurysms with wide necks or unfavorable neck-to-fundus ratos,which have been traditionally considered as poor candidates for Guglielmi detach... 详细信息

关键词：血管疾病血管畸形造影诊断检查方式