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ABF2, ABF3, and ABF4 Promote ABA-Mediated Chlorophyll Degradation and Leaf Senescence by Transcriptional Activation of Chlorophyll Catabolic Genes and Senescence-Associated Genes in Arabidopsis

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Molecular Plant 2016年第9期9卷 1272-1285页

作者： Shan Gao Jiong Gao Xiaoyu Zhu Yi Song zhongpeng li Guodong Ren Xin Zhou Benke Kuai State Key Laboratory of Genetic Engineering School of Life Sciences Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering Institute of Biodiversity ScienceFudan University Shanghai 200438 China These authors contributed equally to this article.

Chlorophyll （Chl） degradation is an integral process of leaf senescence, and NYE1/SGR1 has been demonstrated as a key regulator of Chl catabolism in diverse plant species. In this study, using yeast one-hybrid screening, we identified three abscisic acid （ABA）-responsive element （ABRE）-binding transcription factors, ABF2 （AREB1）, ABF3, and ABF4 （AREB2）, as the putative binding proteins of the NYE1 promoter. Through the transactivation analysis, electrophoretic mobility shift assay, and chromatin immunoprecipitation, we demonstrated that ABF2, ABF3, and ABF4 directly bound to and activated the NYE1 promoter in vitro and in vivo. ABA is a positive regulator of leaf senescence, and exogenously applied ABA can accelerate Chl degradation. The triple mutant of the ABFs, abf2abf3abf4, as well as two ABA-insensitive mutants, abil-1 and snrk2.2/2.3/2.6, exhibited stay-green phenotypes after ABA treatment, along with decreased induction of NYE1 and NYE2 expression. In contrast, overexpression of ABF4 accelerated Chl degradation upon ABA treatment. Interestingly, ABF2/3/4 could also activate the expression of two Chl catabolic enzyme genes, PAO and NYCl, by directly binding to their promoters. In addition, abf2abf3abf4 exhibited a functional stay-green phenotype, and senescence-associated genes （SAGs）, such as SAG29 （SWEET15）, might be directly regulated by the ABFs. Taken together, our results suggest that ABF2, ABF3, and ABF4 likely act as key regulators in mediating ABA-triggered Chl degradation and leaf senescence in general in Arabidopsis.

关键词： abscisic acid ABF transcription factor chlorophyll degradation leaf senescence Arabidopsisthaliana

REF6 promotes lateral root formation through de-repression of PIN1/3/7 genes

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Journal of Integrative Plant Biology 2019年第4期61卷 383-387页

作者： Xiaolei Wang Jiong Gao Shan Gao zhongpeng li Benke Kuai Guodong Ren State Key Laboratory of Genetic Engineering and Fudan Center for Genetic Diversity and Designing Agriculture Institute of Plant Biology School of Life Sciences Fudan University School of life Sciences Qilu Normal University

The H3K27 methyltransferase CLF inhibits lateral root(LR) formation through depositing the repressive H3K27me3 mark to the chromatin of PIN1, a key polar auxin transporter gene. Here, we show that the H3K27me3 demethylase REF6 promotes lateral root primordium initiation and LR emergence. REF6 directly binds to the chromatin of PIN1/3/7. Dysfunction in REF6 results in increased levels of H3K27me3 on PIN1/3/7 and suppressed expression of PIN genes. Genetic analysis of the clf ref6 double mutant revealed an antagonistic action between CLF and REF6, in terms of LR *** findings indicate that H3K27 methylation and demethylation activities are likely coordinated to ensure proper LR organogenesis.

关键词： REF6 PIN1/3/7 genes^FA

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连续梁倒拆施工倾覆稳定性的体系可靠度方法

同济大学学报（自然科学版） 2015年第8期43卷 1167-1173页

作者：马如进李忠鹏同济大学土木工程学院上海200092

针对连续梁双悬臂挂篮逆施工顺序拆除施工的纵向倾覆稳定性,采用可靠度方法对其进行安全评价.提出因果链模式的体系可靠度方法,推导拓展了全概率公式的应用范围,并据此推导了因果链模式体系可靠度计算公式,计算结果和串并联联接模式很... 详细信息

针对连续梁双悬臂挂篮逆施工顺序拆除施工的纵向倾覆稳定性,采用可靠度方法对其进行安全评价.提出因果链模式的体系可靠度方法,推导拓展了全概率公式的应用范围,并据此推导了因果链模式体系可靠度计算公式,计算结果和串并联联接模式很好吻合.推导拓展了贝叶斯公式的应用范围,分析得出导致体系失效最主要的失效模式.对最主要的失效模式进行了参数敏感性分析,得出可以指导拆除施工抗倾覆问题的结论,即吊索强度、根数和钢管桩强度以及切割不对称误差对拆除体系倾覆安全可靠度影响非常大.

关键词：混凝土连续梁倒拆施工纵向倾覆稳定性可靠度因果链模式

Proposal for experimentally observing expectant ball lightning

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Chinese Optics Letters 2021年第8期19卷 116-120页

作者： Silin Guo zhongpeng li Chuliang Zhou Ye Tian State Key Laboratory of High Field Laser Physics and CAS Center for Excellence in Ultra-Intense Laser Science Shanghai Institute of Optics and Fine MechanicsChinese Academy of SciencesShanghai 201800China Center of Materials Science and Optoelectronics Engineering University of Chinese Academy of SciencesBeijing 100049China

Ball lightning is widely concerning because it is hard to detect,predict,and *** dependences of electromagnetic(EM)solitons,which are considered expectant ball lightning,forming at the wavelength of the incident light are investigated with two-dimensional particle-in-cell *** shows that both the long wavelength microwave and the short wavelength laser are not suitable for producing the observed ball-lightning-like EM solitons.A strong field terahertz wave is proposed to inject and generate EM *** paper can provide some references for researchers studying ball lightning.

关键词： expectant ball lightning electromagnetic solitons terahertz

NON-YELLOWING2 （NYE2）, a Close Paralog of NYE1, Plays a Positive Role in Chlorophyll Degradation in Arabidopsis

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Molecular Plant 2016年第4期9卷 624-627页

作者： Wu, Shouxin li, zhongpeng Yang, lifeng Xie, Zuokun Chen, Junyi Zhang, Wei liu, Tianqi Gao, Shan Gao, Jiong Zhu, Yihua Xin, Jiwen Ren, Guodong Kuai, Benke Fudan Univ Sch Life Sci Fudan Inst Plant Biol State Key Lab Genet Engn Shanghai 200438 Peoples R China Huazhong Agr Univ Coll Plant Sci & Technol Natl Key Lab Crop Genet Improvement Wuhan 430070 Peoples R China

Dear Editor,Plant senescence is an integral part of plant development, with old organs senescing in association with the emergence and development of nascent ones. Rapid chlorophyll （Chl） degradation, the most prominent event during green organ senescence, is proposed to be a key detoxification process to facilitate massive nutrient remobilization （lim et al., 2007）.

关键词： senescence integral development

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抗震救灾精神的制度基础

中华文化论坛 2008年第3期 98-100页

作者：李忠鹏四川省社会科学院宏观经济与工业经济研究所

汶川抗震救灾中,当代中国人所表现出的高度动员力、凝聚力和整合力,所表现出的大勇大爱、大仁大义,与旧时代国民性中的冷漠、麻木、自私,形成了鲜明的对比。这种抗震救灾精神,具有较为坚实的内在制度基础。要使这种精神进一步固化,应该... 详细信息

汶川抗震救灾中,当代中国人所表现出的高度动员力、凝聚力和整合力,所表现出的大勇大爱、大仁大义,与旧时代国民性中的冷漠、麻木、自私,形成了鲜明的对比。这种抗震救灾精神,具有较为坚实的内在制度基础。要使这种精神进一步固化,应该继续推进社会主义民主政治建设,完善市场经济制度,加强市民社会建设。

关键词：抗震救灾精神固化制度基础

TOR kinase coordinates nutrient with hormone signaling to orchestrate plant growth,development and stress tolerance

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TOR kinase coordinates nutrient with hormone signaling to or...

基层党组织领导新型农村集体经济发展的生成逻辑、作用机制与优化路径

2018全国植物生物学大会

作者： zhongpeng li Pengcheng Wang liwen Fu Jian-Kang Zhu Yan Xiong Basic Forestry and Proteomics Research Center Haixia Institute of Science and TechnologyFujian Agriculture and Forestry University Shanghai Center for Plant Stress Biology Chinese Academy of Science Center for Excellence in Molecular Plant SciencesChinese Academy of Sciences

Sessile plants need to adjust their growth/stress tolerance mode constantly depending on the intensely changing ***,energy and hormone signaling mechanisms constitute the most ancient and fundamental regulatory networks in all life *** growth/stress plasticity is activated and switched by nutrient and hormone signaling remains enigmatic in photosynthetic *** target of rapamycin(TOR)kinase is an evolutionarily conserved master regulator that integrates energy,nutrients,growth factors and stress signals to promote survival and growth in all *** previous work has revealed that TOR integrates both nutrient and growth hormone signals to tightly control cell proliferation in meristems to stimulate plant *** can sense metabolic sugar signal and auxin level to modulate root and shoot meristem activation by phosphorylating cell cycle related E2 F transcription ***,we also found that plant stress hormone,ABA(Abscisic acid),strongly inhibited TOR kinase *** inhibition effect of ABA is dependent on the canonical ABA signaling ***2 s,the key downstream effector of ABA signaling,directly binds,phosphorylates Raptor(one of the components of TOR complex) to inhibit TOR kinase ***,our findings established a pivotal hub role of TOR signaling in integrating different environmental signals,nutrient status with plant growth and stress hormones to orchestrate plant growth and fitness.

关键词： glucose hormone target of rapamycin phosphorylation cell proliferation stress tolerance

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农村经济 2024年第4期 1-12页

作者：李忠鹏王伟西安交通大学马克思主义学院陕西西安710049 华北电力大学马克思主义学院北京市102206

党建引领是新型农村集体经济发展壮大的政治引擎。新时代基层党组织领导新型农村集体经济发展是对马克思主义农民合作经济思想这一科学理论的根本遵循,是总结党领导农民发展集体经济实践经验而提出的创新举措,更是在新的历史起点上全面... 详细信息

党建引领是新型农村集体经济发展壮大的政治引擎。新时代基层党组织领导新型农村集体经济发展是对马克思主义农民合作经济思想这一科学理论的根本遵循,是总结党领导农民发展集体经济实践经验而提出的创新举措,更是在新的历史起点上全面推进乡村振兴、巩固党在农村执政基础、推动农业农村现代化的现实需要。党的政治优势与组织优势,集体产权、经营体制等方面的制度改革优势和乡土社会的资源禀赋转化为新型农村集体经济的实际发展效能,需要通过社会动员、组织统筹、市场经营、利益联结、协商议事等有效作用机制,从而全面提升基层党组织促成村民集体行动、谋划产业发展方向、加强农村市场建设、推动集体收益共享、构建多元共治格局的统合治理能力。在新发展阶段,要通过加强对新型集体经济组织的领导、创新新型集体经济发展的方式、提升集体经济民主治理效能来强化基层党组织对新型集体经济的统领作用。

关键词：党建引领新型农村集体经济乡村振兴共同富裕乡村治理

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基于螺旋接触线的五轴侧铣刀具定位方法

工程设计学报 2024年第5期31卷 575-584页

作者：李忠朋张立强刘钢上海工程技术大学机械与汽车工程学院上海201620 机械工业航空大型复杂薄壁构件智能制造技术重点实验室上海201620 成都智远先进制造技术研究院四川成都610511

针对五轴侧铣加工非可展直纹面时刀具与设计曲面相互干涉的问题,提出了一种基于螺旋接触线的刀具定位方法。首先,基于Z-buffer法建立单个刀位下的误差解析模型,以评估刀具定位方法的优劣。其次,根据非可展直纹面的扭转特性,构造刀轴矢... 详细信息

针对五轴侧铣加工非可展直纹面时刀具与设计曲面相互干涉的问题,提出了一种基于螺旋接触线的刀具定位方法。首先,基于Z-buffer法建立单个刀位下的误差解析模型,以评估刀具定位方法的优劣。其次,根据非可展直纹面的扭转特性,构造刀轴矢量的数学模型,同时分析刀具-工件接触线的性质及参数表达式。再次,考虑实际加工中的非线性加工误差,通过运动学变换对全局刀位进行路径插补优化。最后,基于改进两点偏置法、最小二乘法及所提出的方法进行仿真分析并对比3种方法所产生的误差,同时基于后2种方法进行实验验证。仿真和实验结果表明,所提出的方法能够有效减小侧铣加工中的原理误差,可为非可展直纹面的五轴侧铣加工提供一定的参考依据。

关键词：五轴侧铣非可展直纹面螺旋接触线路径优化原理误差