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Postnatal development of rat retina:a continuous observation and comparison between the organotypic retinal explant model and in vivo development

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Neural Regeneration Research 2025年第3期20卷 900-912页

作者： Baoqi Hu Rui Wang Hanyue Zhang Xiou Wang Sijia Zhou Bo Ma yan Luan xin Wang xinlin Chen Zhichao Zhang Qianyan Kang Department of Ophthalmology The First Affiliated Hospital of Xi’an Jiaotong UniversityXi’anShaanxi ProvinceChina Department of Ophthalmology The First Affiliated Hospital of Northwest UniversityXi’anShaanxi ProvinceChina Institute of Neurobiology Xi’an Jiaotong University Health Science CenterXi’anShaanxi ProvinceChina

The organotypic retinal explant culture has been established for more than a decade and offers a range of unique advantages compared with in vivo experiments and cell ***,the lack of systematic and continuous comparison between in vivo retinal development and the organotypic retinal explant culture makes this model controversial in postnatal retinal development ***,we aimed to verify the feasibility of using this model for postnatal retinal development studies by comparing it with the in vivo *** this study,we showed that postnatal retinal explants undergo normal development,and exhibit a consistent structure and timeline with retinas in ***,we used SOX2 and PAX6 immunostaining to identify retinal progenitor *** then examined cell proliferation and migration by immunostaining with Ki-67 and doublecortin,***-67-and doublecortin-positive cells decreased in both in vivo and explants during postnatal retinogenesis,and exhibited a high degree of similarity in abundance and distribution between ***,we used Ceh-10 homeodomain-containing homolog,glutamate-ammonia ligase(glutamine synthetase),neuronal nuclei,and ionized calcium-binding adapter molecule 1 immunostaining to examine the emergence of bipolar cells,Müller glia,mature neurons,and microglia,*** timing and spatial patterns of the emergence of these cell types were remarkably consistent between in vivo and explant *** study showed that the organotypic retinal explant culture model had a high degree of consistency with the progression of in vivo early postnatal retina *** findings confirm the accuracy and credibility of this model and support its use for long-term,systematic,and continuous observation.

关键词： bipolar cells differentiation in vivo microglia Müller glia organotypic retinal explant culture postnatal retina development proliferation retinal progenitor cells

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雷电流测量用大型Rogowski线圈输出波形振荡现象分析

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电波科学学报 2010年第6期25卷 1085-1089页

作者：周璧华朱凯鄂李炎新郭建明解放军理工大学工程兵工程学院江苏南京210007

针对大型Rogowski线圈输出波形的振荡现象,在讨论自积分Rogowski线圈工作原理的基础上,建立模型分析了Rogowski线圈的频率响应特性以及振荡产生的主要原因。分析结果表明:线圈的屏蔽层与线圈间、线圈匝间存在的分布电容导致了输出波形... 详细信息

针对大型Rogowski线圈输出波形的振荡现象,在讨论自积分Rogowski线圈工作原理的基础上,建立模型分析了Rogowski线圈的频率响应特性以及振荡产生的主要原因。分析结果表明:线圈的屏蔽层与线圈间、线圈匝间存在的分布电容导致了输出波形的振荡。实验证实,减小该分布电容可使振荡明显变小,从而为改进柔性大型Rogowski线圈的屏蔽设计提供了依据。

关键词： Rogowski线圈自积分分布电容振荡

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Single-cell omics: experimental workflow, data analyses and applications

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Science China life Sciences 2025年第1期68卷 5-102页

作者： Fengying Sun Haoyan li Dongqing Sun Shaliu Fu Lei Gu xin Shao Qinqin Wang xin Dong Bin Duan Feiyang xing Jun Wu Minmin Xiao Fangqing Zhao Jing-Dong J.Han Qi liu Xiaohui Fan Chen li Chenfei Wang Tieliu Shi Department of Clinical Laboratory the Affiliated Wuhu Hospital of East China Normal University (The Second People's Hospital of Wuhu City) Pharmaceutical Informatics Institute College of Pharmaceutical SciencesZhejiang University Key Laboratory of Spine and Spinal Cord Injury Repair and Regeneration (Tongji University) Ministry of EducationOrthopaedic DepartmentTongji HospitalBioinformatics DepartmentSchool of Life Sciences and TechnologyTongji University Frontier Science Center for Stem Cells School of Life Sciences and TechnologyTongji University Translational Medical Center for Stem Cell Therapy and Institute for Regenerative Medicine Shanghai East HospitalBioinformatics DepartmentSchool of Life Sciences and TechnologyTongji University Research Institute of Intelligent Computing Zhejiang Lab Shanghai Research Institute for Intelligent Autonomous Systems Center for Single-cell Omics School of Public HealthShanghai Jiao Tong University School of Medicine National Key Laboratory of Chinese Medicine Modernization Innovation Center of Yangtze River DeltaZhejiang University Center for Bioinformatics and Computational Biology Shanghai Key Laboratory of Regulatory Biologythe Institute of Biomedical Sciences and School of Life SciencesEast China Normal University Beijing Institutes of life Science Chinese Academy of Sciences Peking-Tsinghua Center for life Sciences Academy for Advanced Interdisciplinary StudiesCenter for Quantitative Biology (CQB)Peking University Zhejiang Key Laboratory of Precision Diagnosis and Therapy for Major Gynecological Diseases Women's HospitalZhejiang University School of Medicine Key Laboratory of Advanced Theory and Application in Statistics and Data Science-MOE School of StatisticsEast China Normal University

Cells are the fundamental units of biological systems and exhibit unique development trajectories and molecular features. Our exploration of how the genomes orchestrate the formation and maintenance of each cell, and control the cellular phenotypes of various organismsis, is both captivating and intricate. Since the inception of the first single-cell RNA technology, technologies related to single-cell sequencing have experienced rapid advancements in recent years. These technologies have expanded horizontally to include single-cell genome, epigenome,proteome, and metabolome, while vertically, they have progressed to integrate multiple omics data and incorporate additional information such as spatial sc RNA-seq and CRISPR screening. Single-cell omics represent a groundbreaking advancement in the biomedical field,offering profound insights into the understanding of complex diseases, including cancers. Here, we comprehensively summarize recent advances in single-cell omics technologies, with a specific focus on the methodology section. This overview aims to guide researchers in selecting appropriate methods for single-cell sequencing and related data analysis.

关键词： single-cell sequencing genome epigenome proteomics metabolomics multimodal spatial transcriptomics CRISPR screening

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地震波超前勘探技术在断层探测中的应用与研究

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煤炭技术 2017年第11期36卷 173-175页

作者：梁博森李彦欣山东科技大学矿业与安全工程学院山东青岛266590 山东科技大学数学与系统科学学院山东青岛266590

基于地震波超前勘探理论,利用MATLAB编程模拟得到工作面前方介质弹性波传播特征和规律,从而对工作面前方是否含有断层做出判断,因此利用地震波超前勘测技术探明工作面是否有断层等异常体对煤矿安全生产具有非常重要的意义。

关键词：地震波超前勘探断层工作面

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ZnCl_2/Al_2O_3催化尿素和1,2-丙二醇合成碳酸丙烯酯

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化学世界 2010年第3期51卷 159-161页

作者：王敏张恒李言信赵斌曲阜师范大学化学与化工学院山东曲阜273165

以Al2O3为载体,采用浸渍蒸发法制备了负载型ZnCl2/Al2O3固体酸催化剂,将其应用于催化尿素和1,2-丙二醇合成碳酸丙烯酯,采用XRD、FTIR等手段分析了ZnCl2与载体Al2O3的结合情况,考察了原料配比、反应温度、催化剂用量、反应时间等条件对... 详细信息

以Al2O3为载体,采用浸渍蒸发法制备了负载型ZnCl2/Al2O3固体酸催化剂,将其应用于催化尿素和1,2-丙二醇合成碳酸丙烯酯,采用XRD、FTIR等手段分析了ZnCl2与载体Al2O3的结合情况,考察了原料配比、反应温度、催化剂用量、反应时间等条件对反应效果的影响。结果表明,在最佳反应条件下,碳酸丙烯酯的收率可达68.2%。

关键词： ZnCl2 固体酸催化碳酸丙烯酯

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Inter‑Skeleton Conductive Routes Tuning Multifunctional Conductive Foam for Electromagnetic Interference Shielding,Sensing and Thermal Management

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Nano-Micro Letters 2025年第3期17卷 19-36页

作者： Xufeng li Chunyan Chen Zhenyang li Peng Yi Haihan Zou Gao Deng Ming Fang Junzhe He xin Sun Ronghai Yu Jianglan Shui Caofeng Pan Xiaofang liu School of Materials Science and Engineering Beihang UniversityBeijing 100191People’s Republic of China Institute of Atomic Manufacturing Beihang UniversityBeijing 100191People’s Republic of China Tianmushan Laboratory Xixi Octagon CityYuhang DistrictHangzhou 310023People’s Republic of China Science and Technology On Electromagnetic Scattering Laboratory Beijing Institute of Environmental FeaturesBeijing 100854People’s Republic of China

Conductive polymer foam(CPF)with excellent compressibility and variable resistance has promising applications in electromagnetic interference(EMI)shielding and other integrated functions for wearable ***,its insufficient change amplitude of resistance with compressive strain generally leads to a degradation of shielding performance during ***,an innovative loading strategy of conductive materials on polymer foam is proposed to significantly increase the contact probability and contact area of conductive components under *** inter-skeleton conductive films are constructed by loading alginate-decorated magnetic liquid metal on the polymethacrylate films hanged between the foam skeleton(denoted as AMLM-PM foam).Traditional point contact between conductive skeletons under compression is upgraded to planar contact between conductive ***,the resistance change of AMLM-PM reaches four orders of magnitude under ***,the inter-skeleton conductive films can improve the mechanical strength of foam,prevent the leakage of liquid metal and increase the scattering area of EM ***-PM foam has strain-adaptive EMI shielding performance and shows compression-enhanced shielding effectiveness,solving the problem of traditional CPFs upon *** upgrade of resistance response also enables foam to achieve sensitive pressure sensing over a wide pressure range and compression-regulated Joule heating function.

关键词： Inter-skeleton conductive films Conductive polymer foam liquid metal Electromagnetic interference shielding

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Trinuclear gold-catalyzed site-selective alkylation of peptides

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Science China Chemistry 2025年第1期68卷 249-256页

作者： Qing-Yun Fang Chengyihan Gu Yinghan Chen Wencheng yan Yong liang Weipeng li Chengjian Zhu Jie Han Jin Xie State Key Laboratory of Coordination Chemistry Jiangsu Key Laboratory of Advanced Organic Materials Chemistry and Biomedicine Innovation Center (Chem BIC) School of Chemistry and Chemical Engineering Nanjing University Green Catalysis Center College of Chemistry Zhengzhou University State Key Laboratory of Natural Medicines China Pharmaceutical University

Selective modification of peptides has always been a challenging issue. The selective alkylation of peptides can alter the biological activity and physical properties of molecules, and it has gained significant research interest. We herein disclose an intriguing gold-catalyzed alkylation of peptides using bench-stable unactivated alkyl bromides under photoredox catalysis. A wide range of structurally diverse primary, secondary and tertiary alkyl bromides serve as effective coupling partners to precisely connect with the α-C(sp3)–H in glycine residue of peptides. This protocol demonstrates an exceptionally broad scope for alkyl halides and excellent tolerance for a wide range of useful functional groups. A series of relay transformations to construct cyclic peptides, to proceed click reaction and to realize peptide fluorescence labeling further enhance its synthetic practicality. In addition, mechanistic studies and density functional theory(DFT) calculations reveal an inner-sphere and subsequent outersphere single electron transfer(SET) mechanism.

关键词： gold catalysis peptide alkylation C-C coupling radical

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关中地区历史时期蝗灾统计及其影响浅析

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干旱区资源与环境 2006年第5期20卷 159-162页

作者：殷淑燕黄春长仇立慧李新艳贾耀锋陕西师范大学旅游与环境学院西安710062

历史时期关中地区蝗灾的发生频率具有明显的波动性,且随着历史的发展,有在波动中上升的趋势。唐朝中期以前,关中地区蝗灾的发生频率较低,波动幅度也较小,之后,关中地区蝗灾发生频率的波动幅度明显增大,蝗灾的发生次数明显增多。唐朝后期... 详细信息

历史时期关中地区蝗灾的发生频率具有明显的波动性,且随着历史的发展,有在波动中上升的趋势。唐朝中期以前,关中地区蝗灾的发生频率较低,波动幅度也较小,之后,关中地区蝗灾发生频率的波动幅度明显增大,蝗灾的发生次数明显增多。唐朝后期(801-850A.D.)、明末清初(1601-1650 A.D.)和清末至民国(1851-1950 A.D.)是关中地区历史上蝗灾记载最多的时期。与水灾的发生频率相比较,关中地区的蝗灾与旱灾的发生频率表现出更强的一致性,尤其是900AD(唐末)后,每一个蝗灾频率高峰几乎都与旱灾频率高峰完全同步,说明在关中地区蝗灾的发生与旱灾具更强的相关性。

关键词：关中地区历史时期蝗灾

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中性框架与医疗决策框架下博弈实验测量医生利他性的比较研究

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中国卫生政策研究 2023年第7期16卷 48-55页

作者：李心言林兴滕佳利张高源韩优莉首都医科大学公共卫生学院北京100069

目的:比较两种框架下利用博弈实验测量医生利他性的异同,为利他性测量方法选择和量化分析提供参考依据。方法:分别运用中性框架与医疗决策框架博弈实验两种方式测度个体利他性,招募162名医学生在两种框架下进行决策。中性框架下基于社... 详细信息

目的:比较两种框架下利用博弈实验测量医生利他性的异同,为利他性测量方法选择和量化分析提供参考依据。方法:分别运用中性框架与医疗决策框架博弈实验两种方式测度个体利他性,招募162名医学生在两种框架下进行决策。中性框架下基于社会偏好模型计算利他参数,医疗决策框架下包含竞争和不竞争实验场景,基于随机效用模型与固定效应模型计算利他参数。结果:受试者利他性存在一定异质性。中性框架下40%的受试者利他参数大于0.5;医疗决策框架下混合logit模型与条件logit模型利他参数(边际替代率)估计结果具有一致性,不竞争(竞争)场景下48.6%(94.5%)医生利他参数大于1,说明受试医学生相对于个人(医生)利润更重视他人(患者)效益。中性框架和医疗决策框架下不竞争场景测度利他参数显著正相关(Spearman’s ρ=0.2917,P<0.001)。结论:两种框架下测量的医学生均有较高的利他性,中性框架医生利他性相对稳定,医疗决策框架下实验场景的变化显著影响利他参数的数值。

关键词：利他性偏好实验室实验中性框架医疗决策框架

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基于地球化学的地质重金属监测技术及问题分析

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世界有色金属 2020年第9期45卷 227-228页

作者：李延鑫甘肃交通职业技术学院公路桥梁系甘肃兰州730070

由于地质重金属监测技术对解决土壤重金属污染问题具有重要作用,为此提出基于地球化学的地质重金属监测技术及问题分析。利用地质化学测量方法对监测样品进行采集将样品带入实验室进行光谱处理,利用液相色谱-原子荧光联用法和微波消解... 详细信息

由于地质重金属监测技术对解决土壤重金属污染问题具有重要作用,为此提出基于地球化学的地质重金属监测技术及问题分析。利用地质化学测量方法对监测样品进行采集将样品带入实验室进行光谱处理,利用液相色谱-原子荧光联用法和微波消解法对处理后的样品进行监测分析,以此完成对基于地球化学的地质重金属监测技术研究。

关键词：地球化学地质重金属监测技术液相色谱-原子荧光联用法

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