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Investigation of Protein Formulation Process by Light Scattering Detection

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Investigation of Protein Formulation Process by Light Scatte...

Association between physical activity and cardiovascular risk factors:Dose and sex matter

2005年全国高分子学术论文报告会

作者： mark C．Stochl Arthur ley Trevor Havard Dyax Corp． 300 Technology SquareCambridgeMA 02139 Dyax Corp． 300 Technology SquareCambridgeMA 02139 Precision Detectors Inc．34 Williams WayBellinghamMA 02019

＜正＞Aggregate formation is an important concern in the purification,formulation,and storage of protein *** integrity of protein preparations is often monitored by HPLC using UV and RI ***,the characterization of aggregates present at very low levels poses a significant challenge to current HPLC *** we describe the use of HPLC with UV／RI

关键词： Light scattering protein aggregation formulation

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Journal of Sport and Health Science 2021年第5期10卷 604-606页

作者： Alejandro Santos-Lozano Alberto Torres Barran Pablo Fernandez-Navarro Pedro L.Valenzuela Adrian Castillo-Garcia Luis MRuilope David Rios Insua Jose M.Ordovas Victoria ley Alejandro Lucia i+HeALTH Department of Health SciencesEuropean University Miguel de CervantesValladolid 47012Spain Research Institute of the Hospital 12 de Octubre(imas12 PaHerg group)and(CIBER en Fragilidad y Envejecimiento Saludable-CIBEFES)Madrid 28041Spain Institute of mathematical Sciences(ICmaT-CSIC) Madrid 28049Spain d Cancer and Environmental Epidemiology Unit National Center for EpidemiologyCarlos III Institute of HealthMadrid 28029Spain Consortium for Biomedical Research in Epidemiology&Public Health(CIBER en Epidemiologia y Salud Publica-CIBERESP) Madrid 28029Spain Department of Systems Biology University of AlcalaMadrid 28801Spain Fissac-Physiology Health and Physical ActivityMadrid 28801Spain Hypertension Unit and Cardiorenal Translational Laboratory Research Institute of the Hospital 12 de Octubre(imas12)Madrid 28041Spain Jean mayer USDA Human Nutrition Research Center on Aging at Tufts University BostonMA 02111USA Spanish State Research Agency Ministry of ScienceInnovation and UniversitiesMadrid 28046Spain Faculty of Sport Sciences Universidad Europea de MadridMadrid 28670Spain

Dear Editor,ETadegn TagedPFailure to meet World Health Organization(WHO)-determined minimum physical activity(PA)levels is an established risk factor for cardiovascular disease(CVD),^(1)but evidence is still scarce on its effects on other CVD risk ***,whether or not there are sex-specific effects on the association between PA and CVD risk is a matter of controversy,with some authors observing a protective effect of PA in men but not in women,^(2)and others finding the opposite trend.^(3)

关键词： cardiovascular protective finding

Experimental studies on perturbed acoustic resonant spectroscopy by a small rock sample in a cylindrical cavity

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Science China(Physics,Mechanics & Astronomy) 2006年第6期49卷 683-701页

作者： CHEN Dehua1,2,WANG Xiuming2,3,CONG Jiansheng1,XU Delong1,SONG Yanjie1 & ma Shuilong4 1. Department of Geophysics,Daqing Petroleum Institute,Daqing 163318,China 2. Institute of Acoustics,Chinese Academy of Sciences,Beijing 100080,China 3. Division of Petroleum,Australian Commonwealth Scientific and Industrial Research Organization,Bent-ley WA 6102,Australia 4. Daqing Testing and Technology Services Corporation,Daqing 163153,China Department of Geophysics Daqing Petroleum Institute Daqing 163318 China Institute of Acoustics Chinese Academy of Sciences Beijing 100080 China Division of Petroleum Australian Commonwealth Scientific Industrial Research Organization Bentley WA 6102 Australia Daqing Testing Technology Services Corporation Daqing 163153 China

A measurement system for acoustic resonant spectroscopy (ARS) is estab-lished,and the effects of resonant cavity geometry,inner perturbation samples and envi-ronmental temperature on the ARS are investigated. The ARSs of the small samples with various sizes and acoustic properties are measured. The results show that at the normal pressure,the resonant frequency decreases gradually with the increase of liquid tem-perature in the cylindrical cavity,while the resonant amplitude increases. At certain pres-sure and temperature,both the resonant frequency and the amplitude decrease greatly when there exist air bubbles inside the cavity fluid. The ARS is apparently affected by the sample porosity and the sample location in the resonant cavity. At the middle of the cavity,the resonant frequencies reach their maximum values for all of the measurement samples. The resonant frequencies of the porous rock samples are smaller than those of the com-pacted samples if other acoustic parameters are the same. As the sample is moved from the top to the middle of the cavity along its axis,the resonant amplitude increases gradu-ally for the compacted rocks while decreases for the unconsolidated rocks. Furthermore,the resonant amplitude increases firstly and then decreases if the porosity of the rock sample is relatively small. In addition,through the comparisons between the experimental and theoretical results,it is found that the effects of the acoustic parameters and sizes of the samples and the size of the cylindrical cavity on the laboratory results agree well with the theoretical ones qualitatively. These results may provide basic reference for the ex-periment study of rock acoustic properties in a low frequency using ARS.

关键词： acoustic resonant spectroscopy,acoustic velocity and attenuation,experiment measurement,rock acoustic property.

Histo-blood group antigens in Crassostrea gigas and binding profiles with GⅡ.4 Norovirus

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Journal of Oceanology and Limnology 2018年第4期36卷 1383-1391页

作者： ma Liping LIU Hui SU Laij in ZHAO Feng ZHOU Deqing DUAN Delin Key Laboratory of Experimental marine Biology Institute of OceanologyChinese Academy of SciencesQingdao 266071China Key Laboratory for Sustainable Utilization of marine Fisheries Resources Ministry of AgricultureYellow Sea Fisheries Research InstituteChinese Academy of Fishery SciencesQingdao 266071China Laboratory for marine Biology and Biotechnology Qingdao National Laboratory for Marine Science and TechnologyQingdao 266071China

Noroviruses(NoVs) are the main cause of viral gastroenteritis outbreaks worldwide, and oysters are the most common carriers of NoV contamination and transmission. NoVs bind specifically to oyster tissues through histo-blood group antigens(HBGAs), and this facilitates virus accumulation and increases virus persistence in oysters. To investigate the interaction of HBGAs in Pacific oysters with GⅡ.4 NoV, we examined HBGAs with ELISAs and investigated binding patterns with oligosaccharide-binding assays using P particles as a model of five GⅡ.4 NoV capsids. The HBGAs in the gut and gills exhibited polymorphisms. In the gut, type A was detected(100%), whereas type Leb(91.67%) and type A(61.11%) were both observed in the gills. Moreover, we found that seasonal NoV gastroenteritis outbreaks were not significantly associated with the specific HBGAs detected in the oyster gut and gills. In the gut, we found that strain-2006 b and strain-96/96 US bound to type A and H1 but only weakly bound to type Leb; in contrast, the Camberwell and Hunter strains exhibited weak binding to types H1 and ley, and strain-Sakai exhibited no binding to any HBGA type. In the gills, strain-96/96 US and strain-2006 b bound to type Leb but only weakly bound to type H1; strains Camberwell, Hunter, and Sakai did not bind to oyster HBGAs. Assays for oligosaccharide binding to GⅡ.4 NoV P particles showed that strain-95/96 US and strain-2006 b strongly bound to type A, B, H1, Leb, and ley oligosaccharides, while strains Camberwell and Hunter showed weak binding ability to type H1 and ley oligosaccharides and strain-Sakai showed weak binding ability to type Leb and ley oligosaccharides. Our study presents new information and enhances understanding about the mechanism for NoV accumulation in oysters. Further studies of multiple NoV-tissue interactions might assist in identifying new or improved strategies for minimizing contamination, including HBGA-based attachment inhibition or depuration.

关键词： Crassostrea gigas norovirus histo-blood group antigen binding

Reconstruction of vector static magnetic field by different axial NV centers using continuous wave optically detected magnetic resonance in diamond

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Chinese Physics B 2019年第4期28卷 337-341页

作者： Jian-Feng Ye Zheng Jiao Kun ma Zhi-Yong Huang Hai-Jiang Lv Feng-Jian Jiang School of Information Engineering Huangshan University

We carried out a proof-of-principle demonstration of the reconstruction of a static vector magnetic field involving adjacent three nitrogen-vacancy(NV) sensors with corresponding different NV symmetry axes in a bulk diamond. By means of optical detection of the magnetic resonance(ODMR) techniques, our experiment employs the continuous wave(CW) to monitor resonance frequencies and it extracts the information of the detected field strength and polar angles with respect to each NV frame of reference. Finally, the detected magnetic field relative to a fixed laboratory reference frame was reconstructed from the information acquired by the multi-NV sensor.

关键词： diamond defect optical detection magnetic resonance magnetometer

不同放牧强度对荒漠草原几种牧草一些生理指标的影响

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干旱地区农业研究 2008年第2期26卷 79-84页

作者：马红彬宁夏大学西北退化生态系统恢复与重建省部共建教育部重点实验室

研究了不同放牧强度下荒漠草原长芒草(Stipa bungeana)、牛枝子(Lespedeza potanimii)、赖草(ley-mus secalinus)、中亚白草(Pennisetum flaccidum)和糙隐子草(Cleistogenes squarrosa)5种牧草叶片游离脯氨酸(Pro)、丙二醛(MDA)和根系可溶性糖(WSS)、还原糖的含量以及叶片超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)3种保护酶活性的变化。结果表明:随着放牧强度的增加5种牧草各生理指标均有不同程度的变化,其中赖草Pro含量呈先升后降变化,其它牧草呈不断上升趋势;长芒草和糙隐子草分别在放牧强度为1.05和1.50只/hm2时SOD活性最高,分别是不放牧的1.86和2.13倍,两牧草保护酶活性较高且协同性较好,MDA含量较低且增幅不大,WSS含量呈先降后升变化;中亚白草保护酶活性在放牧轻时居中但放牧重时变小,赖草POD活性与SOD和CAT无明显的一致性,反映在MDA含量上两牧草均上升显著,在1.50只/hm2放牧强度时分别是不放牧的4.68和3.89倍,WSS含量均不断降低;牛枝子保护酶活性相对较低,MDA含量最低且增幅较小,WSS含量呈先降后升变化;糙隐子草和牛枝子根系还原糖含量变化与WSS含量变化相同,其它牧草这二者没有明显的相关性。以上述生理指标判断各牧草的适宜放牧强度,则长芒草和糙隐子草放牧强度应不大于1.05只/hm2、赖草不大于0.45只/hm2、中亚白草不大于0.60只/hm2,牛枝子放牧强度可达1.50只/hm2。

关键词：荒漠草原放牧强度牧草生理指标

Effect of semen extender supplementation with trehalose,vitamin C and E on post-thaw min pig sperm qualities

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Effect of semen extender supplementation with trehalose,vita...

流域尺度下地形属性对土壤质地类型变异的影响——以重庆市彭水县一小流域为例

第七届中国畜牧科技论坛

作者： ma hong Liu di Wang wentao Wang liang Fu bo Li zhongqiu He xinmiao Heilongjiang Academy of Agricultural Sciences

(Background) Cryopreservation of min pig sperm cryopreservation is an effective method to protect this valuable genetic resource.(Objective) To determine whether the extender supplementation with some antioxidants is able to improve the quality of post-thawed sperm of min pig.(materials And Methods) Ejaculated min pig sperm frozen in lactose-egg yolk(ley) extender supplemented with trehalose,vitamin C,and/or water-soluble vitamin E were thawed,and quality of sperm were evaluated.(Results) The post-thawed sperm qualities were higher in the supplementation groups than that in the control group(P<0.05).Furthermore,the addition of 100 mM trehalose and 25 mM water-soluble vitamin E to the freezing extender strongly preserved the quality of the sperm(P<0.05).(Conclusion) Trehalose and water-soluble vitamin E in the freezing extender effectively improved post-thaw qualities of min pig sperm.

关键词： sperm quality cryopreservation min pig

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农业资源与环境学报 2019年第3期36卷 279-286页

作者：马冉刘洪斌武伟西南大学资源环境学院重庆400716 重庆市数字农业重点实验室重庆400716 西南大学计算机与信息科学学院重庆400716

为探究流域尺度下地形属性对土壤质地类型变异的影响,以西南地区重庆市彭水县的一个流域为研究区,基于成土母质和地形因子,运用分类回归树(Classification and regression tree,CART)模型,对研究区1165个样点数据进行分析。结果表明:成... 详细信息

为探究流域尺度下地形属性对土壤质地类型变异的影响,以西南地区重庆市彭水县的一个流域为研究区,基于成土母质和地形因子,运用分类回归树(Classification and regression tree,CART)模型,对研究区1165个样点数据进行分析。结果表明:成土母质和地形因子均对土壤质地类型有重要影响,相对重要性较高的因子排序:成土母质(PAR)>风效应(WindEff)>地形湿度指数(SAGATWI);不同母质、不同质地之间地形因子存在显著差异,风效应(WindEff)和漫射辐射(DiffuseIns)分别是奥陶系和志留系母质条件下影响土壤质地分类的相对重要性最高的地形因子;流域尺度CART模型解释率为86.3%,说明CART模型对土壤质地分类精度高。研究表明,基于成土母质和地形因子构建的模型解释效果好,影响显著,可为该地区土壤利用区划和管理提供科学依据。

关键词：流域尺度 CART模型母质地形因子土壤质地类型变异

不同土壤生境下羊草叶片SPAD值与营养品质研究

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宁夏农林科技 2015年第4期56卷 32-36页

作者：马甜中国科学院遗传与发育生物学研究所北京100101 中国科学院植物研究所北京100093

用SPAD-502叶绿素仪采用野外原位、快速、准确测定不同土壤生境下人工种植5个羊草(leymus chinensis)品系叶片SPAD值,化验其营养成分。结果表明,同一土壤生境下5个羊草品系SPAD值间有显著差异;不同土壤生境下,除中科2号羊草Y1品系SPAD... 详细信息

用SPAD-502叶绿素仪采用野外原位、快速、准确测定不同土壤生境下人工种植5个羊草(leymus chinensis)品系叶片SPAD值,化验其营养成分。结果表明,同一土壤生境下5个羊草品系SPAD值间有显著差异;不同土壤生境下,除中科2号羊草Y1品系SPAD值之间无显著差异之外,羊草BG-2、B品系的SPAD值之间差异显著;主要营养成分与SPAD值相关性排序为粗蛋白(CP)>中性洗涤纤维(NDF)>磷(P)>粗灰分(ASH)>干物质;羊草SPAD值与氨基酸总量、必需氨基酸量回归关系极显著。建议将羊草叶片SPAD值作为牧草营养品质鉴别指示值之一。除此之外,不同土壤生境下人工种植中科2号羊草Y1和Y3品系的SPAD值、干物质、粗蛋白、氨基酸总量、必需氨基酸比例、能量等均高于其他品系,粗纤维含量却相对较低,说明中科2号羊草Y1、Y3品系优良的遗传性状比较稳定,不易受到环境条件的影响而改变,是值得大力推广种植的羊草新品系。有灌溉条件的壤土生境,是培育优质羊草的最佳生境条件。

关键词：中科2号羊草 SPAD值营养品质氨基酸总量必需氨基酸