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Evaluation of DNA Extraction Methods for Detection of <i>Leishmania</i>by Polymerase Chain Reaction

American Journal of Molecular Biology 2020年第4期10卷 265-272页

作者： letícia surian batalini Silvana de Oliveira Castro Carla Geórgia Rodrigues Guimarães Souto Herintha Coeto Neitzke-Abreu Manoel Sebastião da Costa Lima-Junior Faculty of Health Sciences Universidade Federal da Grande Dourados (UFGD) Dourados Brazil Postgraduate Program of Health Sciences Universidade Federal da Grande Dourados (UFGD) Dourados Brazil Aggeu Magalhã es Institute Oswaldo Cruz Fundation (Fiocruz) Recife Brazil

There are several methods used to obtain DNA from cells;however, the quantity, integrity, and purity of DNA vary among the methods, which may interfere with the polymerase chain reaction (PCR) results. The objective was to determine the most efficient and cost-effective method that provides the best DNA yield and PCR results. Three methods of DNA isolation were compared: 20% sodium dodecyl sulfate (SDS), guanidine isothiocyanate-phenol-chloroform (GTPC), and DNA extraction using a commercial kit (GE Healthcare GenomicPrep Blood DNA Isolation KitTM). Human peripheral blood samples were inoculated with 104 promastigotes of Leishmania infantum. DNA was quantified and PCR was performed with 13A/13B primers. The results showed that a higher DNA yield was obtained using the GTPC technique (214.51 ng/μL), followed by SDS (26.16 ng/μL) and the commercial kit (10.99 ng/μL). We concluded that while all of the techniques were effective for obtaining DNA, the GTPC method provided the best yield and the brightest bands.

关键词： Leishmania DNA Purification PCR Diagnosis

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Effects of Welding Procedure on Corrosion Resistance and Hydrogen Embrittlement of Supermartensitic Stainless Steel Deposits

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Journal of Iron and Steel Research International 2013年第12期20卷 124-132页

作者： Zappa Sebastián surian Estela Svoboda Hernán Research Secretariat Faculty of EngineeringNational University of Lomas de Zamora DEYTEMA-Development and Technology of Materials Centre Regional Faculty San NicolásNational Technological University Materials and Structures Laboratory INTECINFaculty of EngineeringNational University of Buenos Aires CONICET-National Scientific and Technical Research Council

The effects of shielding gas and post weld heat treatment on the pitting resistance, stress corrosion crack- ing and hydrogen embrittlement of supermartensitic stainless steel deposits were studied. Two all-weld-metal test coupons were prepared using a metal-cored wire under Ar＋ 5% He and Ar＋18%CO2 gas shielding mixtures. Solubi- lizing and solubilizing plus double tempering heat treatments were done with the objective of achieving different mi crostructural results, The samples welded under Ar＋5% He showed higher pitting corrosion resistance, for all post weld heat treatments, than those welded under Ar＋18% CO2. The different post weld heat treatments generated higher susceptibility to this corrosion mechanism. None of the samples presented signs of stress corrosion cracking, but in those subjected to the heat treatment, grain boundary selective attack was observed, on the surfaces of all the samples studied. The samples with highest hardness were more susceptible to hydrogen damage, thereby leading to reduced tensile strength on this condition.

关键词： supermartensitic stainless steel shielding gas post weld heat treatment corrosion hydrogen embrittle ment

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肾小球疾病与妊娠

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国外医学.泌尿系统分册 1985年第1期 27-29页

作者： M surian 林冰若

86例经活检证实的肾小球疾病患者,共有123次妊娠,研究了其临床病程。在妊娠期开始有肾病的有35个病人,超过半数以上无并发症。其他1/3的并发症是属于产科的或胎儿意外,1/3有肾病表现(高血压或肾功能损害),1/3原因两者兼有。其中并发症... 详细信息

86例经活检证实的肾小球疾病患者,共有123次妊娠,研究了其临床病程。在妊娠期开始有肾病的有35个病人,超过半数以上无并发症。其他1/3的并发症是属于产科的或胎儿意外,1/3有肾病表现(高血压或肾功能损害),1/3原因两者兼有。其中并发症发生率最低的是膜性肾病,最高是膜增殖性肾小球肾炎。有6次晚期自然流产,6次死产与5个新生儿死亡。17次早产,7个胎儿比妊娠期龄小。24次妊娠有高血压其中13次是可逆的,与先兆子痫有关。11例持续至分娩后(11个妊娠中5个病人有IgA肾病)。10例妊娠期肾功能恶化。其中6例可逆4例是进行性的(2例有膜增殖性肾小球肾炎)估计大多数病人的肾小球疾病妊娠期自然病史不改变。

关键词：膜性肾病自然病史肾小球自然流产肾功能损害肾活检先兆子痫肾功能恶化冷球蛋白血症活检标本

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