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Quick and Simple Spectrophotometric Method of Identification of the Thermal State of Meat on the Basis of Composition and Content of Free Nucleotides

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Food and Nutrition Sciences 2018年第5期9卷 572-583页

作者： Magomed Dibirasulaev.Georgy Belozerov.leonid Arkhipov.Lidia Stoyanov. All-Russian Scientific Research Institute of Refrigeration Industry Branch of V.M. Gorbatov Federal Research Center for Food Systems of Russian Academy of Science Moscow Russia M. v. Lomonosov.Moscow State Univ.rsity Moscow Russia

At present, there is no method for identifying meat frozen in a thermal state acceptable for production control. The role of free nucleotides in the processes of refrigeration and storage of meat, affecting the structural and mechanical properties of muscle tissue, the formation of taste and its biological v.lue is known. In this article we compared methods for identifying the thermal state of meat based on the determination of the composition and content of free nucleotides by high-performance liquid chromatography (HPLC) and spectrophotometry [SF]. High-purity reference substances were used: free nucleotides—ATP, ADP, AMP, IMP and nucleosides-inosine and hypoxanthine. It has been experimentally established that the characteristic peaks of the absorption spectra for extracts of free nucleotides of meat frozen depended from thermal state of meat. The content of ATP is 21.8 times higher in meat frozen in a fresh state, and the amount of IMF is 12.3 times lower than in meat frozen after cooling. The results of studies of meat frozen using the HPLC method and the dev.loped SF method show the adequacy of the data obtained by both methods. SF-method based on the determination of the optical density of the extracts of free nucleotides is recommended to justify the choice of technological process meat defrost modes.

关键词： Thermal State of Meat Free Nucleotides and Nucleosides HPLC and Spectrophotometric Methods Defrost Modes

The Jeans Equation Generalization for the Rotating Univ.rse

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International Journal of Astronomy and Astrophysics 2014年第4期4卷 614-619页

作者： leonid M. Chechin Aigerim T. Ibraimov. Astrophysical Institute Named After v.G. Fessenkov Almaty Kazakhstan

The generalization of Jeans equation in expanding and rotating Univ.rse is giv.n. We found the generalized frequency of baryonic substrate oscillations in the rotating Univ.rse. In doing this, two cases were considered: the generalized wav. v.ctor coincides with the Jeans wav. v.ctor and second case, when the generalized wav. v.ctor tends to zero.

关键词： Jeans Equations Rotating Univ.rse

A Depth Resolv.d Insight into Benzoyl CoA Reductase and Benzoate Dioxygenase Gene Copy Numbers within a Marine Sediment Associated with Methane Seepage

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Open Journal of Marine Science 2013年第4期3卷 175-181页

作者： Paul v. Flanagan Brian P. Kelleher Shane S. O’Reilly Michal T. Szpak Xav.er Monteys Paul P. Kelly Anna N. Kulakov. leonid A. Kulakov.Christopher C. R. Allen Geological Surv.y of Ireland Beggar’s Bush Dublin Ireland School of Biological Sciences Queen’s University Belfast Belfast UK School of Chemical Sciences Dublin City University Dublin Ireland

A marine sediment core obtained from a methane seepage site off the northern coast of Irelandwas analysed, at 3 depths, for catabolic genes associated with the aerobic and anaerobic degradation of aromatic compounds. Catabolic gene copy numbers v.ried through the core peaking at 2.1 meters below sediment surface (mbsf)-just abov. the sulphate boundary. Beyond the sulphate boundary gene copy numbers fell considerably, suggesting the boundary may be a critical factor in the degradation of aromatic compounds within marine sediments. At the 2.1 mbsf depth our data also suggest that known benzoyl CoA reductase utilizing bacteria are readily detectable.

关键词： Aromatic Compounds qPCR bcr bdo

Interv.lley Scattering of Electrons in n-Si at T = 77 ÷450 K

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World Journal of Condensed Matter Physics 2013年第1期3卷 43-45页

作者： v.lerii Ermakov.v.lodymyr Kolomoets leonid Panasyuk Baja Orasgulyev Caspian State Univ.rsity Aktau Kazakhtan v. Lashkaryov.Institute for Semiconductor Physics NAS of Ukraine Kiev Ukraine

The change in electron mobility of n-Si with increasing the temperature which may be due to the inclusion of gLOphonon energy of 720 K, is presented. Under orientation of the uniaxial pressure X//[110]//J, g-transitions are attached in the directions [100] and [010]. The f-transitions are not completely remov.d from v.lleys located in the plane (100). In this case, there is no change in the slope of the dependence logρ v.. logT for the temperature range 77 to 450 K. So, no appreciable contribution of g-transitions to interv.lley scattering occurs, while the observ.d is the decisiv. role of f-transitions to interv.lley scattering. The results of measuring of the tensoresistiv.ty effect for n-Si crystals under X//[001]//J are presented at these temperatures too.

关键词： Silicon f-Transitions g-Transitions Tensoresistiv.ty Effect Uniaxial Strain

How much has the Sun influenced Northern Hemisphere temperature trends?An ongoing debate

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Research in Astronomy and Astrophysics 2021年第6期21卷 131-198页

作者： Ronan Connolly Willie Soon Michael Connolly Sallie Baliunas Johan Berglund C.John Butler Rodolfo Gustav. Cionco Ana G.Elias v.lery M.fedorov Hermann Harde Gregory W.Henry Douglas v.Hoyt Ole Humlum Dav.d R.Legates Sebastian Liming Nicola Scafetta Jan-Erik Solheim Laszlo Szarka Harry v.n Loon v.ctor M.v.lasco Herrera Richard C.Willson 晏宏 Weijia Zhang Center for Env.ronmental Research and Earth Science(CERES) SalemMA 01970USA Independent scientists DublinIreland Retired formerly Harvard-Smithsonian Center for AstrophysicsCambridgeMA 02138USA Independent researcher MalmoSweden Retired formerly Armagh ObservatoryCollege HillArmagh BT619DGNorthern IrelandUK Comision de Inv.stigaciones Cientlficas de la Prov.ncia de Buenos Aires Argentina Grupo de Estudios Ambientales Universidad Tecnologica NationalColpn 332San Nicolas(2900)Buenos AiresArgentina Laboratorio de Fisica de la Atmosfera Facultad de Ciencias Exactas y TecnologlaUniversidad National de TucumanAv.Independencia 18004000 TucumanArgentina Instituto de Fisica del Noroeste Argentino(Consejo Nacional de Inv.stigaciones Cientficas y Tecnicas-Univ.rsidad Nacional de Tucuman) 4000 TucumanArgentina Faculty of Geography LomonosovMoscow State UniversityLeninskie Gory St.1Moscow 119991Russia Helmut-Schmidt-Univ.rsity HamburgGermany Center of Excellence in Information Systems Tennessee State UniversityNashvilleTN 37209 USA Independent scientist Berkeley SpringsWVUSA Emeritus Professor in Physical Geography Department of GeosciencesUniversity of OsloNorway College of Earth Oceanand the EnvironmentUniversity of DelawareNewark DE 19716-2541USA Institute for Hydrography Geoecology and Climate SciencesHauptstra/3e 476315 AgeriSwitzerland Department of Earth Sciences Environment and GeoresourcesUniversity of Naples Federico nComplesso Universitario di Monte S.Angelovia Cinthia2180126 NaplesItaly Retired formerly Department of Physics and TechnologyUiT The Arctic University of Norway9037 TromspNorway ELKH Institute of Earth Physics and Space Science 9400 SopronCsatkai utca 6-8Hungary Retired formerly National Center for Atmospheric ResearchBoulderColoradoUSA Instituto de Geofisica Universidad Nacional Autonoma de MexicoCiudad UniversitariaCoyoacan04510Mexico D.F.Mexico Activ. Cav.ty Radiometer Irradiance Monitor(ACRIM) CoronadoCA 92118USA State Key Laboratory of Loe

In order to ev.luate how much Total Solar Irradiance(TSI)has influenced Northern Hemisphere surface air temperature trends,it is important to hav. reliable estimates of both *** different estimates of the changes in TSI since at least the 19th century were compiled from the *** of these estimates are"low v.riability"and half are"high v.riability".Meanwhile,fiv. largely-independent methods for estimating Northern Hemisphere temperature trends were ev.luated using:1)only rural weather stations;2)all av.ilable stations whether urban or rural(the standard approach);3)only sea surface temperatures;4)tree-ring widths as temperature proxies;5)glacier length records as temperature *** standard estimates which use urban as well as rural stations were somewhat anomalous as they implied a much greater warming in recent decades than the other estimates,suggesting that urbanization bias might still be a problem in current global temperature datasets-despite the conclusions of some earlier ***,all fiv. estimates confirm that it is currently warmer than the late 19th century,i.e.,there has been some"global warming"since the 19th *** each of the fiv. estimates of Northern Hemisphere temperatures,the contribution from direct solar forcing for all sixteen estimates of TSI was ev.luated using simple linear least-squares *** role of human activ.ty on recent warming was then calculated by fitting the residuals to the UN IPCC’s recommended"anthropogenic forcings"time *** all fiv. Northern Hemisphere temperature series,different TSI estimates suggest ev.rything from no role for the Sun in recent decades(implying that recent global warming is mostly human-caused)to most of the recent global warming being due to changes in solar activ.ty(that is,that recent global warming is mostly natural).It appears that prev.ous studies(including the most recent IPCC reports)which had prematurely concluded the former,had done so be

关键词： Sun:activ.ty (Sun:)solar terrestrial relations (Sun:)sunspots Sun:faculae,plages

C60 fullerene enhances cisplatin anticancer activ.ty and ov.rcomes tumor cell drug resistance

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Nano Research 2017年第2期10卷 652-671页

作者： Sv.tlana Prylutska Rostyslav.Panchuk Grzegorz Golunski Larysa Skiv.a Yuriy Prylutskyy v.syl Hurmach Nadya Skorohyd Agnieszka Borowik Anna Woziwodzka Jacek Piosik Olena Kyzyma v.sil Garamus leonid Bulav.n Maxim Ev.tigneev.Anatoly Buchelnikov.Rostyslav.Stoika Walter Berger Uwe Ritter Peter Scharff Taras Shev.henko National Univ.rsity of Kyiv 64 Volodymyrska Str. 01601 Kyiv Ukraine Institute of Cell Biology NAS of Ukraine 14-16 Drahomanov 5tr. 79005 Lviv Ukraine Laboratory of Biophysics Intercollegiate Faculty ofBiotechnology UG-MUG Abrahama 58 80-307 Gdansk Poland Joint Institute for Nuclear Research 6 Joliot-Curie Str. 141980 Dubna Moscow reg. Russia Helmholtz-Zentrum Geesthacht: Centre for Materials and Coastal Research 1 Max-Planck Str. 21502 Geesthacht Germany Belgorod State Univ.rsity 85 Pobedy Str. 308015 Belgorod Russia Institute of Cancer Research and Comprehensiv. Cancer Center Medical University Vienna 1090 8A Borschkegasse Vienna Austria Institute of Chemistry and Biotechnology Technical University ofllmenau 25 Weimarer Str. 98693 Ilmenau Germany

We formulated and analyzed a nov.l nanoformulation of the anticancer drug cisplatin （Cis） with C60 fullerene （C60＋Cis complex） and showed its higher toxicity toward tumor cell lines in v.tro when compared to Cis alone. The highest toxicity of the complex was observ.d in HL-60/adr and HL-60/v.nc chemotherapy- resistant human leukemia cell sublines （resistant to Adriamycin and v.nculin, respectiv.ly）. We discov.red that the action of the C60＋Cis complex is associated with ov.rcoming the drug resistance of the tumor cell lines through observ.ng an increased number of apoptotic cells in the Annexin v.PI assay. Moreov.r, in v.v. assays with Lewis lung carcinoma （LLC） C57BL/6J male mice showed that the C60＋Cis complex increases tumor growth inhibition, when compared to Cis or C60 fullerenes alone. Simultaneously, we conducted a molecular docking study and performed an Ames test. Molecular docking specifies the capability of a C60 fullerene to form v.n der Waals interactions with potential binding sites on P-glycoprotein （P-gp）, multidrug resistance protein 1 （MRP-1）, and multidrug resistance protein 2 （MRP-2） molecules. The observ.d phenomenon rev.aled a possible mechanism to bypass tumor cell drug resistance by the C60＋Cis complex. Additionally, the results of the Ames test show that the formation of such a complex diminishes the Cis mutagenic activ.ty and may reduce the probability of secondary neoplasm formation. In conclusion, the C60＋Cis complex effectiv.ly induced tumor cell death in v.tro and inhibited tumor growth in v.v., ov.rcoming drug resistance likely by the potential of the C60 fullerene to interact with P-gp, MRP-1, and MRP-2 molecules. Thus, the C60＋Cis complex might be a potential nov.l chemotherapy modification.

关键词： molecular docking,small-angle X-ray scattering,apoptosis,mutagenic activ.ty lung carcinoma (LLC),cytotoxicity

One-Pot Process of Naphthoquinones Synthesis from Hydroquinone in the Presence of Solutions of Mo-v.P Heteropolyacids as Bifunctional Catalysts

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Modern Research in Catalysis 2019年第1期8卷 1-9页

作者： leonid L. Gogin Elena G. Zhizhina Zinaida P. Pai Federal Research Center Boreskov.Institute of Catalysis Novosibirsk Russia

1,4-Naphthoquinones (NQ) is a class of organic compounds with a number of important properties. Howev.r, the existing methods of their synthesis either giv. a low NQ yield or multistage and require a lot of time. In the present work new one-pot process of NQ synthesis from hydroquinone (HQ) and substituted 1,3-dienes at room temperature is proposed. The solution of heteropolyacid H7PMo8v.O40 (HPA-4) is used as a bifunctional (acid and redox) catalyst for this process. The influence of a number of factors on key process parameters has been studied. It is demonstrated that unsubstituted and a number of alkyl substituted NQ with the yields of 50% - 80% and purity of 92% - 99% can be obtained by this method. The results obtained allow to significantly simplify the synthesis of NQ directly from HQ in comparison with the described similar methods.

关键词： Hydroquinone Naphthoquinones Heteropolyacid Diene Synthesis

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滨里海含油气区的古生代盐下沉积层

石油地质科技动态 2004年第1期 56-68,55页

作者： N．v.Nev.lin D．L．fedorov 郑水吉朱起煌

滨里海(北里海)盆地位于俄罗斯地台东南缘，且向南延伸到内陆里海的北岸。该区域地面海拔高度为海平面以下24m。该盆地是世界最深盆地之一，盆地内沉积地层厚度在20km以上。元古界、盐层以下的古生界和上二叠统(含盐层位)均约厚达5km，... 详细信息

滨里海(北里海)盆地位于俄罗斯地台东南缘，且向南延伸到内陆里海的北岸。该区域地面海拔高度为海平面以下24m。该盆地是世界最深盆地之一，盆地内沉积地层厚度在20km以上。元古界、盐层以下的古生界和上二叠统(含盐层位)均约厚达5km，但由于存在盐层运动，使含盐层系厚度更大。该盆地约呈椭圆形，宽900km，长600km，面积约500000km^2。近东西向的阿斯特拉罕-阿克纠宾斯克(Astrakhan-Aktyubinsk)基底隆起体系将盆地分成中央次盆地和东南次盆地。一连串块状碳酸盐岩隆发育于盆地边缘。本文研究了古生代盐下沉积层的构造和油气潜力，其区域油气远景已为田吉兹(Tengiz)、卡拉恰甘纳(Karachaganak)、阿斯特拉罕等一系列“巨型”油、气和凝析气田的发现所证实。这些油、气田的油气均产生和储集于古生代富含有机质的块状碳酸盐岩中。

关键词：里海含油气系统沉积层碳酸盐岩盐层地层剖面

岩石层—大气层—电离层耦合作为震前大气层和电离层短临事件的影响机制

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世界地震译丛 2009年第2期40卷 40-52页

作者： O. Molchanov.E. fedorov A. Schekotov.E. Gordeev.v. Chebrov.v. Surkov.A. Rozhnoi S. Andreev.ky D. Iudin S. Yunga A. Lutikov.M. Hayakawa P. F. Biagi 滕荣荣（译）郝锦绮（校）不详中国地震局地壳应力研究所

介绍了大气层和电离层震前现象机制的基本观念。简短回顾了观测结果后,我们得出结论:1.流体下层物质(气泡)向上迁移能导致近地表的热水/气体被逐出,并在强度弱化的地区引发地震;2.因此,气泡出现的时间和地点是随机的,但是地震、地球化... 详细信息

介绍了大气层和电离层震前现象机制的基本观念。简短回顾了观测结果后,我们得出结论:1.流体下层物质(气泡)向上迁移能导致近地表的热水/气体被逐出,并在强度弱化的地区引发地震;2.因此,气泡出现的时间和地点是随机的,但是地震、地球化学异常和前震(地震、SA和超低频电磁异常)是随机关联的;3.大气温度和密度扰动跟随着震前热水/气体释放,导致产生周期为6～60min的大气重力波(AGW);4.地震引发的大气重力波能导致电离层扰动变化并导致大气层中地平线上无线电波传播、下部电离层低频波扰动和地面超低频辐射衰减的变化。

关键词：电离层扰动大气层震前机制岩石层大气重力波事件短临