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Reference genes for quantitative RT-PCR data in gastric tissues and cell lines

World Journal of Gastroenterology 2013年第41期19卷 7121-7128页

作者： Fernanda Wisnieski Danielle Queiroz Calcagno Mariana Ferreira Leal leonardo Caires dos santos Carolina de Oliveira Gigek Elizabeth Suchi Chen Thaís Brilhante Pontes Paulo Pimentel Assumpo Mnica Barauna de Assumpo Smia Demachki Rommel Rodríguez Burbano Marília de Arruda Cardoso Smith Disciplina de Genética Departamento de Morfologia e GenéticaUniversidade Federal de So PauloSo Paulo 04023900Brazil Departamento de Ortopedia e Traumatologia Universidade Federal de So PauloSo Paulo 04038031Brazil Núcleo de Pesquisa em Oncologia Hospital Joo de Barros BarretoUniversidade Federal do ParáBelém 60673000Brazil Laboratório de Citogenética Humana Instituto de Ciências BiológicasUniversidade Federal do ParáBelém 66075110Brazil

AIM:To evaluate the suitability of reference genes in gastric tissue samples and cell ***:The suitability of genes ACTB,B2M,GAPDH,RPL29,and 18S rRNA was assessed in21 matched pairs of neoplastic and adjacent nonneoplastic gastric tissues from patients with gastric adenocarcinoma,27 normal gastric tissues from patients without cancer,and 4 cell lines using reverse transcription quantitative real-time polymerase chain reaction(RT-qPCR).The ranking of the best single and combination of reference genes was determined by NormFinder,geNorm,BestKeeper,and *** addition,GenEx software was used to determine the optimal number of reference *** validate the results,the mRNA expression of a target gene,DNMT1,was quantified using the different reference gene combinations suggested by the various software packages for ***:ACTB was the best reference gene for all gastric tissues,cell lines and all gastric tissues plus cell ***+B2M or ACTB+B2M was the best combination of reference genes for all the gastric *** the other hand,ACTB+B2M was the best combination for all the cell lines tested and was also the best combination for analyses involving all the gastric tissues plus cell *** to the GenEx software,2 or 3 genes were the optimal number of references genes for all the gastric *** relative quantification of DNMT1 showed similar patterns when normalized by each combination of reference *** level of expression of DNMT1 in neoplastic,adjacent non-neoplastic and normal gastric tissues did not differ when these samples were normalized using GAPDH+B2M(P=0.32),ACTB+B2M(P=0.61),or GAPDH+B2M+ACTB(P=0.44).CONCLUSION:GAPDH+B2M or ACTB+B2M is the best combination of reference gene for all the gastric tissues,and ACTB+B2M is the best combination for the cell lines tested.

关键词： Gastric cancer Reference gene Normalization Gene expression Quantitative real-time polymerase chain reaction

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骨整合的分形测量和微观建模

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中国口腔医学继续教育杂志 2020年第2期23卷 110-113页

作者： leonardo Cavalcanti Bezerra dos santos Alessandra de Albuquerque Tavares Carvalho Jair Carmeiro Leiao Paulo Jose Duarte Neto Tatijana Stosic Borko Stosic 夏睿迪(译) 张新春(审) 不详中山大学光华口腔医学院 510000

本研究使用分形维数的概念评估了通过模拟体液浸没对钛金属植入物表面进行不同物理化学处理后的骨整合过程。并发现.不同的处理导致磷酸钙晶体的生长模式大不相同.其分形维数的范围为1.68-1.93。所观察到的晶体模式可以通过一般的沉积... 详细信息

本研究使用分形维数的概念评估了通过模拟体液浸没对钛金属植入物表面进行不同物理化学处理后的骨整合过程。并发现.不同的处理导致磷酸钙晶体的生长模式大不相同.其分形维数的范围为1.68-1.93。所观察到的晶体模式可以通过一般的沉积、扩散和聚集生长机制来解释,其中扩散微粒的黏附概率起着基本作用。

关键词：物理化学处理分形维数生长模式骨整合金属植入物微观建模聚集生长分形测量

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Lead and Cadmium Distribution in Tubes of Galvanized Steel by Hot-Dip Used for Drinking Water Supply

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Journal of Civil Engineering and Architecture 2020年第5期14卷 271-279页

作者： Fernando Benedicto Mainier Felipe Silva Semaan Thais Pereira Sarmento Pedro Henrique Oliveira amorim Rodolfo Gomes Shamádos santos Escola de Engenharia Universidade Federal FluminenseNiteróiRio de Janeiro 27255-125Brazil Instituto de Química Universidade Federal FluminenseNiteróiRio de Janeiro 27255-125Brazil

The main objective of this work was to evaluate the presence of lead and cadmium in galvanized steel tubes by hot-dip used exclusively for drinking water *** this research,samples of galvanized tubes were removed from walls of residential buildings with 30 years of *** the process of hot galvanizing,the manufacturing standards allow or for low cost conveniences,and the use of primary zinc ingot with 1.6%metallic impurities,such as lead and cadmium,is allowed,enabling the incorporation of these metals into the zinc coating deposited on the carbon *** associated corrosion of these tubes can release these toxic metals,contaminating the drinking water used by *** samples taken from the tubes were evaluated by a square-wave anodic stripping voltammetry technique,and the results for lead and cadmium suggested randomly distributed levels,indicating that the contents of these contaminants depend mainly on the quality of the added primary zinc and the operational conditions of the immersion *** metallographic evaluations performed on the samples of the galvanized tubes showed irregularities in thickness and even a lack of the zinc layer deposited on the carbon steel *** facts suggest that galvanized tubes should not be used for drinking water.

关键词： Lead zinc hot-dip galvanized steel square-wave anodic stripping voltammetry contamination

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Carbon and Nitrogen in the Soil Profile under the Canopy Dipteryx alata(Baru)in Pasture

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Journal of Life Sciences 2018年第4期12卷 189-194页

作者： Maria Luisa Pegoraro Rodrigues Sebastiao Ferreira de Lima Ana Paula Leite de Lima Natália Pegoraro Rodrigues Maria Aparecida dos santos Amanda amorim da Silva Autonomous graduate in forestry engineeringresearch in silvicultureNova Andradina 79750-000Brazil Agronomy and forestry engineering Federal University of Mato Grosso do SulChapadao do Sul 79560-000Brazil Autonomous graduate in forestry engineeringresearch in silvicultureAlcinópolis 79530-000Brazil PhD student in Terrestrial ecotoxicology at the University of Aveiro/Aarhus Aveiro 3200-628Portugal

The presence of trees in the pastures can bring several benefits to both the animals and to the soil under their ***,the objective of this study was to evaluate the levels of organic carbon and nitrogen under the baru canopy in the soil *** adult baru trees were *** samplings were made at the four cardinal points at four different distance points from the projection radius of the crown,being:P1=one-third of the crown projection radius length;P2=two-thirds of the crown projection radius length;P3=extremity of the crown projection radius;P4=one and a half times the maximum crown projection radius *** samples were collected at each point at depths of 0-0.10,0.10-0.20 and 0.20-0.40 *** carbon and nitrogen contents were evaluated and the C/N ratio was *** baru trees influenced the soil organic and carbon contents in the areas under their *** organic carbon and nitrogen contents were higher in relation to the tree trunk(P1)and also in the most superficial layer of the soil(0-0.10 m).The C/N ratio was higher outside the tree canopy and in the deeper layer.

关键词： Organic matter C/N ratio leguminous trees

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