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Quantitative proteomics analysis of parthenogenetically induced pluripotent stem cells

Protein & Cell 2011年第8期2卷 631-646页

作者： Zhe Hu leiwang Zhensheng Xie Xinlei Zhang Du Feng Fang Wang Bingfeng Zuo Lingling Wang Zhong Liu Zhisheng Chen Fuquan Yang Lin Liu Department of Cell Biology and Genetics Key Laboratory of Bioactive Materials of Ministry of EducationCollege of Life SciencesNankai UniversityTianjin 300071China Laboratory of Proteomics Institute of BiophysicsChinese Academy of SciencesBeijing 100101China Center for Computational and Systems Biology Institute of BiophysicsChinese Academy of SciencesBeijing 100101China

Parthenogenetic embryonic stem(pES)cells isolated from parthenogenetic activation of oocytes and embryos,also called parthenogenetically induced pluripotent stem cells,exhibit pluripotency evidenced by both in vitro and in vivo differentiation *** proteomic analysis was performed using differential in-gel electrophoresis and isotope-coded affinity tag-based quantitative proteomics to investigate the molecular mechanisms underlying the developmental pluripotency of pES cells and to compare the protein expression of pES cells generated from either the in vivo-matured ovulated(IVO)oocytes or from the in vitro-matured(IVM)oocytes with that of fertilized embryonic stem(fES)cells derived from fertilized embryos.A total of 76 proteins were upregulated and 16 proteins were downregulated in the IVM pES cells,whereas 91 proteins were upregulated and 9 were downregulated in the IVO pES cells based on a minimal 1.5-fold change as the cutoff *** distinct pathways were found in the differentially expressed proteins except for those involved in metabolism and physiological ***,no differences were found in the protein expression of imprinted genes between the pES and fES cells,suggesting that genomic imprinting can be corrected in the pES cells at least at the early *** germline competent IVM pES cells may be applicable for germ cell renewal in aging ovaries if oocytes are retrieved at a younger age.

关键词： parthenogenetic embryonic stem cell proteome fluorescent two-dimensional difference in-gel electrophoresis isotope-coded affinity tag

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Detection of micrometastasis of gastric carcinoma in peripheral blood circulation

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World Journal of Gastroenterology 2004年第6期10卷 804-808页

作者： Xi-MeiChen Guo-YuChen Zhi-RongWang Feng-ShangZhu Xiao-leiwang XiaZhang DepartmentofGastroenterology TongjiHospitalofTongjiUniversityShanghai200065China

AIM:To detect the micrometastasis of gastric carcinoma in peripheral blood circulation using immunomagnetic beads sorting technique and RT-PCR technique,and to discuss its significance and the difference between the two ***:Density gradient centrifugation was used to isolate mononuclear cells from peripheral blood,immunomagnetic beads sorting technique and RT-PCR technique were used to detect the disseminated carcinoma ***,immunocytochemical and immunofluorescence staining were also used to identify the characteristics of the cells separated with immunomagnetic beads sorting ***:Cells expressing cytokeratin were separated and enriched from the peripheral blood specimens of patients suffering from gastric carcinoma or chronic gastritis. After HE staining, two kinds of ceils with little cytoplasm were *** of these cells had small and round nuclei, even chromatins and the thickness of nuclear membrane was normal. Immunohistochemical staining indicated that there were CD34 and CD45 expression on the cell membrane ofthis kind of cells and these cells also showed expressed human telomerase reverse transcriptase by immunofluorescence staining, but the expression of carcinoembryonic antigen was ***,these cells might hematopoiesis *** kind of cells had larger and abnormal nuclei with thicker nuclear membranes. Massed chromatins and polynudeoli were found in the nudei. These cells expressed human telomerase reverse transcriptase and carcinoembryonic antigen,but CD34 and CD45 were not found on the cell ***,these cells were considered as gastric carcinoma cells escaping from the original focuses and existing in the peripheral blood *** cells were found in 25 of 60(41.7%) specimens of peripheral blood from patients with gastric carcinoma, while there were no such cells separated from the blood specimens of chronic gastritis *** difference of positive rates of disseminated carcinoma c

关键词：胃癌微小转移病灶外周血逆转录聚合酶链反应淋巴细胞肿瘤诊断学

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Shadow Matching: Improving Smartphone GNSS Positioning in Urban Environments

Shadow Matching: Improving Smartphone GNSS Positioning in Ur...

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第四届中国卫星导航学术年会

作者： leiwang Paul D Groves Marek K Ziebart Dept. Civil Environmental and Geomatic Engineering University College LondonGower Street London WC1E 6BT United Kingdom

Positioning using Global Navigation Satellite Systems （GNSS） is unreliable in dense urban areas. The accuracy in the across-street direction can degrade to a few tens of meters in deep urban canyons because the unobstructed satellite signals travel along the street, rather than across it, resulting in poor signal geometry. A new solution to this cross-street positioning problem is to use 3D city models to predict satellite visibility, and then compare with the measured satellite visibility to determine position. This concept is known as shadow *** this work, for the first time, the shadow-matching technique is demonstrated using GNSS data from a Smartphone. The algorithm has been optimized for speed. The system is then verified with real-world GPS and GLONASS data from Samsung Galaxy S3 smartphones. The experimental data show thatshadowmatching outperforms the conventional GNSS positioning, improving cross-street positioning success rate for a 2 meter accuracy from 18.5 % to 86.9 % of the time at the selected test site. The system is also compatible with Beidou and Galileo, with potentially improved performance.

关键词： GNSS Urban Canyons 3D City Model Shadow Matching Smartphone

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MiR156 regulates anthocyanin biosynthesis through SPL targets and other microRNAs in poplar

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Horticulture Research 2020年第1期7卷 1247-1258页

作者： Yamei Wang Wenwen Liu Xinwei Wang Ruijuan Yang Zhenying Wu HanWang leiwang Zhubing Hu Siyi Guo Hailing Zhang Jinxing Lin Chunxiang Fu Shandong Provincial Key Laboratory of Energy Genetics Key Laboratory of BiofuelsChinese Academy of SciencesQingdao Institute of BioEnergy and Bioprocess TechnologyChinese Academy of SciencesQingdao 266101China University of Chinese Academy of Sciences Beijing 100049China College of Biological Sciences&Biotechnology Beijing Forestry UniversityBeijing 10083China Collaborative Innovation Center of Crop Stress Biology Henan Province and Institute of Plant Stress BiologyHenan UniversityKaifeng 475001China Grass and Science Institute of Heilongjiang Academy of Agricultural Sciences HarbinHeilongjiangChina

Anthocyanins biosynthesized from the flavonoid pathway are types of pigments that are involved in the protection of poplar from biotic and abiotic *** researchers studying anthocyanin-related transcription factors and structural genes in poplar have made significant ***,little is known about the regulatory role of microRNAs in anthocyanin biosynthesis in ***,we overexpressed miR156 in poplar to study the comprehensive effects of the miR156-SPL module on the biosynthesis of *** RNA sequencing analysis revealed 228 microRNAs differentially expressed in transgenic poplar plants with dramatically increased miR156 ***,integrated microRNAomic and transcriptomic analysis suggested that two microRNAs,miR160h,and miR858,have the potential to affect anthocyanin accumulation in poplar by regulating auxin response factors and MYB transcription factors,***,the accumulation of miR160h and miR858 displayed a positive correlation with miR156 levels,suggesting a possible interaction between the miR156-SPL module and these microRNAs in ***,metabolomics analysis revealed that the levels of anthocyanins,flavones,and flavonols were substantially elevated in transgenic poplar plants overexpressing miR156 compared with the wild type,whereas the total lignin content was reduced in the transgenic *** together,our results indicate that miR156 can fine tune the anthocyanin biosynthetic pathway via multiple factors,including microRNAs,transcription factors,and the levels of structural genes,in *** provides additional clues for understanding the complex regulatory network of anthocyanin biosynthesis in woody plants.

关键词： miR156 analysis anthocyanin

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Survival Outcome of Squamous Cell Carcinoma Arising from Sinonasal Inverted Papilloma

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Chinese Medical Journal 2015年第18期128卷 2457-2461页

作者： Qing-Zhuang Liang De-Zhi Li Xiao-leiwang Hui Huang Zhen-Gang Xu Yue-HuangWu Department of Breast and Thyroid Surgery Peking University Shenzhen Hospital Shenzhen Guangdong 518036 China Department of Head and Neck Surgery Cancer Hospital Chinese Academy of Medical Sciences Peking Union Medical College Beijing 100021 China

Background： Sinonasal inverted papilloma （IP） is a rare benign tumor of the nasal cavities and paranasal sinuses. It is destructive or bone-rcmodeling, tends to recur alter surgical resection, and has a significant malignant potential. The present study aimed to pertbnn a retrospective analysis of patients with squamous cell carcinoma （SCC） arising from IP, including characteristics, survival outcome, and predictors of associated malignancy. Methods： The medical records of 213 patients diagnosed with IP from January 1970 to January 2014 were retrospectively reviewed. Eighty-seven patients were diagnosed with SCC/IP; their clinical characteristics, treatments, and survival outcomes were analyzed. Results： Of the 87 patients with SCC/IP, the 5- and 10-year overall survival outcomes were 39.6% and 31.8%, respectively. Twenty-nine of these patients received surgery and 58 received combined surgery and radiation. Of the patients with stages III-IV, the 5-year survival rate was 30.7% for those treated with surgery only and 39.9% for those given the combination treatment （P = 0.849）. Factors associated with significantly poor prognosis were advanced-stage, metachronous tumors, or with cranial base and orbit invasion. Age, synchronous or metachronous tumors, and pathological stage were independent risk factors for mortality, shown by multivariate analysis. Conclusion： Patients with SCC/IP had low overall survival outcomes. Advanced age, stage, and metachronous tumors are the main factors affecting prognosis. Treatment planning should consider high-risk factors to improve survival outcome.

关键词： Sinonasal Inverted Papilloma Squamous Cell Carcinoma Survival Outcome

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Molecular cloning and expression analysis of a hemolin-like molecule from Antheraea pernyi

Molecular cloning and expression analysis of a hemolin-like ...

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第十二届家（柞）蚕遗传育种暨良种繁育学术研讨会

作者： Yu Sun leiwang Cen Qian Lishang Dai Jun Li Congfen Zhang Bao-Jian Zhu Chao-Liang Liu College of Life Science Anhui Agricultural University

Hemolin belongs to the immunoglobulin superfamily and plays an important role in innate immune response of insects. In this study, a hemolin-like cDNA of 1418 bp was obtained from Antheraea pernyi（Ap-hemolin-like）. Sequence analysis revealed Ap-hemolin-like was homologous to those hemolins from other insect species. Recombinant Ap-hemolin-like protein was expressed in Escherichia coli cells, and polyclonal antibodies were produced against the recombinant proteins. Real-time PCR and western blot analysis showed that the Ap-hemolin-like was expressed in hemolymph, Malpighian tubules, midgut, epidermis and fat body, with the highest expression level in hemolymph. To investigate its role in the immune response against microorganisms, fifth instar larvae were challenged by injecting nucleopolyhedrovirus（NPV）, E. coli, or Beauveria bassiana. The results showed that the expression of Ap-hemolin-like in hemolymph and fat body was obviously induced by microorganisms. In addition, the recombinant Ap-hemolin-like protein promoted the agglutination of E. coli in the presence of calcium, which was confirmed by agglutination assay. These results suggested that the Ap-hemolin-like proteinwas involved in innate immune response of A. pernyi against pathogens.

关键词： Antheraea pernyi Hemolin-like Expression pattern Immune response

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重庆市九龙坡区探索企业河长治水思路探索

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生态文明新时代 2023年第4期 65-67页

作者：陈野雷汪不详

河流的治理与管护涉及多个领域,本文以重庆市九龙坡区为例,从涉河排污企业着手,以小见大,讲述行政河长带动企业河长参与治河护河,通过充分发挥沿河企业能动性,帮助企业强化绿色生产意识、扶持企业创新生产方式节能减排增效、借助智慧手... 详细信息

河流的治理与管护涉及多个领域,本文以重庆市九龙坡区为例,从涉河排污企业着手,以小见大,讲述行政河长带动企业河长参与治河护河,通过充分发挥沿河企业能动性,帮助企业强化绿色生产意识、扶持企业创新生产方式节能减排增效、借助智慧手段开展企业尾水深度治理与管护、政企合力处置企业突发水污染事件等措施,突破城市内河治理中水质提升瓶颈,探索出一条既有利于河湖健康成长、又有利于社会效益提高的绿色发展之路。

关键词：企业河长河湖治理绿色发展

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Characterization and function of a short peptidoglycan recognition protein from the Chinese oak silkworm, Antheraea pernyi

Characterization and function of a short peptidoglycan recog...

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第十二届家（柞）蚕遗传育种暨良种繁育学术研讨会

作者： Li-Shang Dai Cen Qian leiwang Guo-Qing Wei Qiu-Ning Liu Yu Sun Cong-Fen Zhang Jun Li Dong-Ran Liu Bao-Jian Zhu Chao-Liang Liu College of Life Science Anhui Agricultural University

Peptidoglycan recognition proteins（PGRPs） are conserved proteins in animals from insects to mammals and play an important role in immune response by recognizing peptidoglycan on microbial surfaces. In this study, a PGRP gene from Antheraea pernyi, named Ap-PGRP-A, was isolated and characterized. Sequence analysis revealed that the full-length c DNA of Ap-PGRP-A was 961 bp, containing a 40 bp 5′-untranslated sequence, a 339 bp 3′-untranslated region and an open reading frame of 582 bp. This gene encoded a putative protein of 193 amino acid residues. Phylogenetic analysis indicated that Ap-PGRP-A had the closest protein sequence similarity to Antheraea mylitta PGRP. The recombinant protein was successfully expressed in Escherichia coli cells and a rabbit anti-Ap-PGRP-A antibody was also prepared. Real-time quantitative reverse transcription-PCR analysis showed that Ap-PGRP-A was extensively expressed in the fat body, midgut, hemocyte, malpighian tubule and epidermis of A. pernyi, especially in the fat body and midgut. The expression levels of Ap-PGRP-A were significantly up-regulated by three types of microorganisms, and Ap-PGRP-A expression was more sensitive in response to the Gram-negative bacterium E. coli than the Gram-positive bacterium Bacillus subtilis or the fungus Beauveria bassiana. These data indicate that Ap-PGRP-A may play a role in the innate immune responses of A. pernyi.

关键词： Antheraea pernyi Peptidoglycan recognition protein Expression Immune response

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早期胃癌内镜特点及高危因素分析

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中国内镜杂志 2020年第10期26卷 48-53页

作者：王磊时军利李炳庆承德医学院附属医院消化内科河北承德067000

目的探讨早期胃癌内镜下表现的高危因素,提高早期胃癌的内镜检出率。方法回顾性分析该院2016年12月-2018年6月行胃镜精查患者的临床病理资料,共245例患者247处病变。记录患者内镜诊断、病变大小、形态、颜色、是否附着黏液、病变部位、... 详细信息

目的探讨早期胃癌内镜下表现的高危因素,提高早期胃癌的内镜检出率。方法回顾性分析该院2016年12月-2018年6月行胃镜精查患者的临床病理资料,共245例患者247处病变。记录患者内镜诊断、病变大小、形态、颜色、是否附着黏液、病变部位、靛胭脂染色情况、窄带成像技术联合放大内镜(NBI-ME)下表现等,先用2检验分析早期胃癌的相关危险因素,再应用多因素Logistic回归模型分析早期胃癌内镜下表现的高危因素,并进行分层分析,同时探讨NBI-ME对早期胃癌的诊断敏感性和特异性。结果 2检验分析结果表明:萎缩性胃炎、病变位于胃窦部、病变形态为凹陷型、病变> 1 cm、白光下存在边界、颜色发红、靛胭脂染色后不着色及存在边界、NBI-ME下观察可见边界、表面微结构不规则、微血管不规则均与早期胃癌有关;Logistic回归分析表明:靛胭脂染色后不着色(O^R=4.474,95%CI:1.576~12.701,P=0.005)、NBI-ME观察存在边界(O^R=7.608,95%CI:1.427~40.570,P=0.017)、NBI-ME观察微血管不规则(O^R=8.051,95%CI:1.509~42.949,P=0.015)为早期胃癌的独立危险因素。分层分析显示:NBI-ME观察微血管不规则联合靛胭脂染色不着色诊断早期胃癌的准确性为78.3%。NBI-ME诊断早期胃癌的敏感性为72.1%,特异性为78.9%。结论内镜下发现病变靛胭脂染色后不着色、NBI-ME观察到微血管不规则或NBI-ME观察到存在边界,提示为早期胃癌可能性较大。其中NBI-ME观察到微血管不规则联合靛胭脂染色不着色诊断早期胃癌的准确度较高。

关键词：早期胃癌多因素Logistic回归分析窄带成像技术放大内镜电子内镜

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甲磺酸阿帕替尼在晚期难治性骨与软组织肉瘤治疗中的临床应用观察

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中国肿瘤临床 2019年第12期46卷 615-621页

作者：康建平肖砚斌董苏伟李文忠马翔张漾杰王雷龚一帆云南省肿瘤医院昆明医科大学第三附属医院骨科

目的:观察甲磺酸阿帕替尼治疗晚期难治性骨与软组织肉瘤的有效性及安全性,同时分析影响患者无进展生存期(progression free survival,PFS)的可能相关因素。方法:选取云南省肿瘤医院2017年6月至2018年9月收治的21例晚期难治性骨与软组织... 详细信息

目的:观察甲磺酸阿帕替尼治疗晚期难治性骨与软组织肉瘤的有效性及安全性,同时分析影响患者无进展生存期(progression free survival,PFS)的可能相关因素。方法:选取云南省肿瘤医院2017年6月至2018年9月收治的21例晚期难治性骨与软组织肉瘤患者,予以甲磺酸阿帕替尼片,主要疗效指标为PFS,次要疗效指标为总生存时间(overall survival,OS)。根据实体瘤疗效评定标准RECIST 1.1评价临床疗效,包括总缓解率(overall response rate,ORR)、疾病控制率(disease control rate,DCR),同时根据美国国家癌症研究所(National Cancer Institute,NCI)4.0标准随访观察安全性。结果:21例患者均获得随访,截至最后1次随访时间2019年3月31日,无完全缓解(complete response,CR),部分缓解(partial response,PR)患者有2例(9.5%),疾病稳定(stable disease,SD)患者7例(33.3%),疾病进展(progressive disease,PD)患者12例(57.1%),ORR为9.5%,DCR为(42.8%),中位无进展生存期(median progression free survival,mPFS)为8个月,中位总生存时间(median overall survival,mOS)为14个月。患者的性别、年龄、ECOG评分、组织来源、是否手术、是否化疗对患者PFS的影响差异无统计学意义(P>0.05),只有服用阿帕替尼前是否有放疗史这一因素对患者PFS的影响差异均具有统计学意义(P<0.05),有放疗史患者的PFS低于无放疗史患者。Ⅲ级及以上不良反应依次为手足综合征(14.3%)、气胸(14.3%)和贫血(4.8%)。结论:甲磺酸阿帕替尼在治疗晚期难治性骨与软组织肉瘤中显现出一定的疗效,不良反应总体来说是可预见、可控制和可逆转的,对于治疗依从性良好、无其他治疗方法可选择的晚期难治性骨与软组织肉瘤的患者可以尝试。

关键词：阿帕替尼骨与软组织肉瘤靶向治疗疗效分析

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