检索结果-南通市图书馆

Effect of Intergranular Precipitation on the Internal Oxidation Behavior of Cr–Mn–N Austenitic Stainless Steels

在线全文

学校读者我要写书评

暂无评论

Acta Metallurgica Sinica(English Letters) 2015年第8期28卷 1008-1014页

作者： lei-gang zheng Xiao-Qiang Hu Xiu-Hong Kang Dian-Zhong Li Shenyang National Laboratory for Materials Science Institute of Metal Research Chinese Academy of Sciences

The effect of intergranular precipitation on the internal oxidation behavior of Cr–Mn–N austenitic steels at 1000 °C in dry air atmosphere was investigated using scanning electron microscope, transmission electron microscope, and X-ray diffraction analysis. The results show that intergranular M23C6 carbide morphologies play an important role on the internal oxidation behavior of Cr–Mn–N steels. During the period of the oxidation, both discontinuous chain-shaped and continuous film-shaped intergranular M23C6 carbides precipitated along the grain boundaries. Internal oxides of silica preferentially intruded into the matrix along grain boundaries with discontinuous M23C6 carbide particles, while silica was obviously restricted at the interfaces between the external scale and matrix on the occasion of continuous film-shaped M23C6 carbides. It is seemed that reasonable microstructure could improve the oxidation resistance of Cr–Mn–N steels.

关键词： M23C6 precipitation Internal oxidation Cr–Mn–N austenitic steel

Sequencing, Polymorphism and Expression Profile Analysis of Porcine HexokinaseⅡ(HK2) Gene

在线全文

学校读者我要写书评

暂无评论

Agricultural Sciences in China 2006年第5期5卷 384-389页

作者： WANG Jun DENG Chang-yan XIONG Yuan-zhu ZUO Bo CHENG Huan-chen LI Feng-e lei Ming-gang zheng Rong JIANG Si-wen Key Laboratory of Swine Genetics and Breeding Ministry of Agriculture/College of Animal Sciences Huazhong Agricultural UniversityWuhan 430070 P.R. China

Hexokinase Ⅱ has been demonstrated to play the role of a key enzyme member in the glycolysis reaction. It catalyzes the conversion of glucose to glucose-6-phosphate, thus committing glucose to the glycolytic pathway. In this paper, the partial exons and introns 10, 11, 13 and 14 of the porcine HK2 gene were cloned and sequenced by comparative genomics. Comparative sequencing of three pig breeds revealed ten putative single-nucleotide polymorphisms （SNPs）, one of which in intron 10 with differing bases （G981A） is within the restriction site for enzyme Msp I. Distribution of Msp I -RFLP genotype and allele frequencies among different pig breeds were studied. By association analysis between Msp I PCR- RFLP polymorphism （AA, AB, BB genotypes of HK2 gene intron 10） and some meat quality and carcass traits in F2 group, which was constructed by our laboratory, a significant difference of pig average backfat at rump was found between AB and BB genotypes （P〈0 05） in F2 group. In addition, the pattern of expression of ilK2 in a variety of tissues in pig was also determined using semi-quantitative RT-PCR. The expression of HK2 mRNA was detected only in pig skeletal muscle.

关键词： hexokinase Ⅱ pig SNPs expression profile skeletal muscle

Joint Probabilistic Scheduling and Resource Allocation for Wireless Networked Control Systems

在线全文

学校读者我要写书评

暂无评论

IEEE/CAA Journal of Automatica Sinica 2025年第1期 258-260页

作者： Meng zheng lei Zhang Wei Liang IEEE the State Key Laboratory of Robotics and the Key Laboratory of Networked Control Systems Shenyang Institute of Automation Chinese Academy of Sciences the University of Chinese Academy of Sciences

Dear Editor,This letter presents a joint probabilistic scheduling and resource allocation method(PSRA) for 5G-based wireless networked control systems(WNCSs). As a control-aware optimization method, PSRA minimizes the linear quadratic Gaussian(LQG) control cost of WNCSs by optimizing the activation probability of subsystems, the number of uplink repetitions, and the durations of uplink and downlink phases. Simulation results show that PSRA achieves smaller LQG control costs than existing works.

关键词：

cDNA Cloning,Sequence Analysis of the Porcine LIM and Cysteine-rich Domain 1 Gene

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Acta Biochimica et Biophysica Sinica 2005年第12期37卷 843-850页

作者： Jun WANG Chang-Yah DENG Yuan-Zhu XIONG Bo ZUO lei XING Feng-E LI Ming-gang lei Rong zheng Si-Wen JIANGKey Laboratiry of Swine Genetics and Breeding,Ministry of Agriculture.College of Animal Sciences,Huazhong Agricultural University.Wuhan 430070,China KeyLaboratoryofSwineGeneticsandBreeding MinistryofAgricultureCollegeofAnimalSciencesHuazhongAgriculturalU

LIM domain proteins are important regulators in cell growth,cell fate determination,celldifferentiation and remodeling of the cell cytoskeleton by their interaction with various structural proteins,kinases and transcriptional *** molecular biology combined with in silico cloning,we havecloned the complete coding sequence of pig LIM and the cysteine-rich domain 1 gene (LMCD1) whichencodes a 363 amino acid *** estimated molecular weight of the LMCD1 protein is 40,788 Da witha pI of *** was found to be highly expressed in both skeletal muscle and cardiac *** revealed that the deduced protein sequence shares 86%,91% and 93% homology with that of itshuman,mouse and rat counterparts,*** LMCD 1 protein was predicted by bioinformaticssoftware to contain a novel cysteine-rich domain in the N-terminal region,two LIM domains in theC-terminal region,nine potential protein kinase C phosphorylation sites,seven casein kinase Ⅱ phosphorylationsites,a tyrosine kinase phosphorylation site,seven N-glycosylation and N-myristoylation sites and a singlepotential N-glycosylation site,which is similar to the protein’s human *** tree wasconstructed by aligning the amino acid sequences of the LIM domain trom different *** addition,fourbase mutations were detected by comparing the sequences of Large White pigs with those of ChineseMeishan *** G294A mutation site was confirmed by polymerase chain reaction-single-strandconformation polymorphism *** allele frequencies were studied in five pig breeds.

关键词： LIM and cysteine-rich domain 1(LMCD1) SSCP PCR gene expression data analysis

Peripheral blood RNA biomarkers can predict lesion severity in degenerative cervical myelopathy

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Neural Regeneration Research 2025年第6期20卷 1764-1775页

作者： Zhenzhong zheng Jialin Chen Jinghong Xu Bin Jiang lei Li Yawei Li Yuliang Dai Bing Wang Department of Spine Surgery The Second Xiangya Hospital of Central South UniversityChangshaHunan ProvinceChina

Degenerative cervical myelopathy is a common cause of spinal cord injury,with longer symptom duration and higher myelopathy severity indicating a worse *** numerous studies have investigated serological biomarkers for acute spinal cord injury,few studies have explored such biomarkers for diagnosing degenerative cervical *** study involved 30 patients with degenerative cervical myelopathy(51.3±7.3 years old,12 women and 18 men),seven healthy controls(25.7±1.7 years old,one woman and six men),and nine patients with cervical spondylotic radiculopathy(51.9±8.6 years old,three women and six men).Analysis of blood samples from the three groups showed clear differences in transcriptomic *** analysis identified 128 differentially expressed genes that were enriched in patients with neurological *** least absolute shrinkage and selection operator analysis,we constructed a five-gene model(TBCD,TPM2,PNKD,EIF4G2,and AP5Z1)to diagnose degenerative cervical myelopathy with an accuracy of 93.5%.One-gene models(TCAP and SDHA)identified mild and severe degenerative cervical myelopathy with accuracies of 83.3%and 76.7%,*** of two immune cell types(memory B cells and memory-activated CD4^(+)T cells)predicted levels of lesions in degenerative cervical myelopathy with 80%*** results suggest that peripheral blood RNA biomarkers could be used to predict lesion severity in degenerative cervical myelopathy.

关键词： biomarkers candidate genes degenerative cervical myelopathy gene expression analysis immune cell types neurological disabilities peripheral blood RNA profiles spinal cord injury

Small extracellular vesicles derived from cerebral endothelial cells with elevated microRNA 27a promote ischemic stroke recovery

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2025年第1期20卷 224-233页

作者： Yi Zhang Zhongwu Liu Michael Chopp Michael Millman Yanfeng Li Pasquale Cepparulo Amy Kemper Chao Li Li Zhang zheng gang Zhang Department of Neurology Henry Ford HospitalDetroitMIUSA Department of Physics Oakland UniversityRochesterMIUSA Department of Pathology Henry Ford HospitalDetroitMIUSA Division of Pharmacology Department of NeuroscienceReproductive and Dentistry SciencesSchool of MedicineUniversity of Naples“Federico Ⅱ”NaplesItaly

Axonal remodeling is a critical aspect of ischemic brain repair processes and contributes to spontaneous functional *** previous in vitro study demonstrated that exosomes/small extracellular vesicles(sEVs)isolated from cerebral endothelial cells(CEC-sEVs)of ischemic brain promote axonal growth of embryonic cortical neurons and that microRNA 27a(miR-27a)is an elevated miRNA in ischemic *** the present study,we investigated whether normal CEC-sEVs engineered to enrich their levels of miR-27a(27a-sEVs)further enhance axonal growth and improve neurological outcomes after ischemic stroke when compared with treatment with non-engineered CEC-sEVs.27a-sEVs were isolated from the conditioned medium of healthy mouse CECs transfected with a lentiviral miR-27a expression *** EVs isolated from CECs transfected with a scramble vector(Scra-sEVs)were used as a *** male mice were subjected to permanent middle cerebral artery occlusion and then were randomly treated with 27a-sEVs or *** array of behavior assays was used to measure neurological *** with treatment of ischemic stroke with Scra-sEVs,treatment with 27a-sEVs significantly augmented axons and spines in the peri-infarct zone and in the corticospinal tract of the spinal grey matter of the denervated side,and significantly improved neurological *** vitro studies demonstrated that CEC-sEVs carrying reduced miR-27a abolished 27a-sEV-augmented axonal *** analysis revealed that 27a-sEVs systemically administered preferentially localized to the pre-synaptic active zone,while quantitative reverse transcription-polymerase chain reaction and Western Blot analysis showed elevated miR-27a,and reduced axonal inhibitory proteins Semaphorin 6A and Ras Homolog Family Member A in the peri-infarct *** of the Clathrin-dependent endocytosis pathway substantially reduced neuronal internalization of *** data provide evidence that 27a-sEVs have

关键词： axonal remodeling cerebral endothelial cells exosomes miR-27a mitochondria Semaphorin 6A small extracellular vesicles stroke

CNKSR2 interactome analysis indicates its association with the centrosome/microtubule system

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2025年第8期20卷 2420-2432页

作者： Lin Yin Yalan Xu Jie Mu Yu Leng lei Ma Yu zheng Ruizhi Li Yin Wang Peifeng Li Hai Zhu Dong Wang Jing Li Institute for Translational Medicine The Affiliated Hospital of Qingdao UniversityMedical CollegeQingdao UniversityQingdaoShandong ProvinceChina School of Basic Medicine Qingdao UniversityQingdaoShandong ProvinceChina College of Life Sciences and School of PharmacyMedical CollegeQingdao UniversityQingdaoShandong ProvinceChina Department of Urology Qingdao Municipal Hospital Affiliated to Qingdao UniversityQingdaoShandong ProvinceChina

The protein connector enhancer of kinase suppressor of Ras 2(CNKSR2),present in both the postsynaptic density and cytoplasm of neurons,is a scaffolding protein with several protein-binding *** of the CNKSR2 gene have been implicated in neurodevelopmental disorders,particularly intellectual disability,although the precise mechanism involved has not yet been fully *** has demonstrated that CNKSR2 plays a role in facilitating the localization of postsynaptic density protein complexes to the membrane,thereby influencing synaptic signaling and the morphogenesis of dendritic ***,the function of CNKSR2 in the cytoplasm remains to be *** this study,we used immunoprecipitation and high-resolution liquid chromatography-mass spectrometry to identify the interactors of *** a combination of bioinformatic analysis and cytological experiments,we found that the CNKSR2 interactors were significantly enriched in the proteome of the *** also showed that CNKSR2 interacted with the microtubule protein DYNC1H1 and with the centrosome marker *** colocalization analysis confirmed the centrosomal localization of *** we downregulated CNKSR2 expression in mouse neuroblastoma cells(Neuro 2A),we observed significant changes in the expression of numerous centrosomal *** manipulation also affected centrosome-related functions,including cell size and shape,cell proliferation,and ***,we found that CNKSR2 interactors were highly enriched in de novo variants associated with intellectual disability and autism spectrum *** findings establish a connection between CNKSR2 and the centrosome,and offer new insights into the underlying mechanisms of neurodevelopmental disorders.

关键词： autism spectrum disorder centrosome CNKSR2 intellectual disability interactome mass spectrometry microtubule neurodevelopmental disease protein complexes protein-protein interactions

在线全文

学校读者我要写书评

暂无评论

防范企业科技人才流失的对策探讨

武汉冶金管理干部学院学报 2009年第1期19卷 28-30页

作者：雷正刚雷三容武汉大学土木建筑工程学院湖北武汉430072 武汉冶金管理干部学院学报编辑部湖北武汉430081

科技人才是提升企业核心竞争力的战略资源和实现企业跨越式发展的关键因素,也是竞争者争夺的最重要的资源。如何有效管理科技人才,激发科技人才创造力,降低科技人才流失率,成为许多企业人力资源管理工作亟待解决的重大问题。对待科技人... 详细信息

科技人才是提升企业核心竞争力的战略资源和实现企业跨越式发展的关键因素,也是竞争者争夺的最重要的资源。如何有效管理科技人才,激发科技人才创造力,降低科技人才流失率,成为许多企业人力资源管理工作亟待解决的重大问题。对待科技人才企业应本着留用为主、预防为辅的目的,将科技人才的管理、激励与预防有机结合起来,有效地防范因科技人才的流失而给企业带来致命的打击,并最终能够促成企业与科技人才的共同发展,双方共赢。

关键词：企业科技人才激励管理流失防范

Activatable chemiluminescence probe based on four-arm PEGconjugated-pyropheophorbide-a for in vivo autofluorescence-free imaging of peroxynitrite

在线全文

学校读者我要写书评

暂无评论

Science China Chemistry 2025年

作者： Jianmei Ke lei Ding Ronggui Lian Cheng zheng Wenjun Li Aixian zheng Yupeng Sun Ming Wu Yongyi Zeng Xiaolong Liu Maochun Hong Xiaolong Zhang The United Innovation of Mengchao Hepatobiliary Technology Key Laboratory of Fujian Province Mengchao Hepatobiliary Hospital of Fujian Medical University Ganjiang Innovation Academy Chinese Academy of Sciences School of Rare Earths University of Science and Technology of China College of Chemistry Fuzhou University State Key Laboratory of Structure Chemistry Fujian Institute of Research on the Structure of Matter Chinese Academy of Sciences

Peroxynitrite（ONOO-） is a highly reactive nitrogen species that plays pivotal roles in cell signal transduction and physiological or pathological progresses. However, commonly used ONOO-optical imaging probes are still hampered by high background/autofluorescence（fluorescence probe）, short emission wavelength, or poor selectivity in the case of chemiluminescence. Herein,we report a facile method to prepare an activatable chemiluminescence probe（PPA-PEG） with good biocompatibility and functionality for in vivo autofluorescence-free imaging of ONOO-. The PPA-PEG consists of pyropheophorbide-a（PPA）, a typical deep red photosensitizer that acts as both the recognition and signaling element, and 4-arm poly（ethylene glycol）(4-arm PEG), which improves the biosafety and water solubility of probe. These components can self-assemble into nanoparticles（namely PPA-PEG nanoprobe） in aqueous solution. The PPA-PEG nanoprobe showed an ultra-low chemiluminescence signal before interacting with ONOO-, but exhibited good selectivity, high sensitivity and a fast response toward ONOO-. The PPAPEG was successfully applied to image cellular ONOO-changes, as well as the endogenous ONOO-changes in inflammation models and subcutaneous or orthotopic hepatocellular carcinoma（HCC） tumors models in living mice. In vitro and in vivo studies verified the good detection and imaging capabilities of PPA-PEG for peroxynitrite, demonstrating suitable tissue penetration and a high signal-to-background ratio（SBR）. Thus, our nanoprobe can serve as a valuable activatable chemiluminescence imaging tool for studying important peroxynitrite-related chemical and biological applications.

关键词：