检索结果-南通市图书馆

Matrix metalloproteinase-9 expression and blood brain barrier permeability in the rat brain after cerebral ischemia/reperfusion injury

引用

Neural Regeneration Research 2008年第5期3卷 505-508页

作者： Lifang lei Xiaohong Zi qiuyun Tu Department of Neurology Third Xiangya Hospital Central South University Changsha 410083 Hunan Province China

BACKGROUND： The integrity of the blood brain barrier （BBB） plays an important role in the patho-physiological process of cerebral ischemia/reperfusion injury. It has been recently observed that metalloproteinase-9 （MMP-9） is closely related to cerebral ischemia/reperfusion injury OBJECTIVE： This study was designed to observe MMP-9 expression in the rat brain after cerebral ischemia/reperfusion injury and to investigate its correlation to BBB permeability. DESIGN, TIME AND SETTING： This study, a randomized controlled animal experiment, was performed at the Institute of Neurobiology, Central South University between September 2005 and March 2006. MATERIALS： Ninety healthy male SD rats, aged 3-4 months, weighing 200-280 g, were used in the present study. Rabbit anti-rat MMP-9 polyclonal antibody （Boster, Wuhan, China） and Evans blue （Sigma, USA） were also used. METHODS： All rats were randomly divided into 9 groups with 10 rats in each group： normal control group, sham-operated group, and ischemia for 2 hours followed by reperfusion for 3, 6, 12 hours, 1, 2, 4 and 7 days groups. In the ischemia/reperfusion groups, rats were subjected to ischemia/reperfusion injury by suture occlusion of the right middle cerebral artery. In the sham-operated group, rats were merely subjected to vessel dissociation. In the normal control group, rats were not modeled. MAIN OUTCOME MEASURES： BBB permeability was assessed by determining the level of effusion of Evans blue. MMP-9 expression was detected by an immunohistochemical method. RESULTS： All 90 rats were included in the final analysis. BBB permeability alteration was closely correlated to ischemia/reperfusion time. BBB permeability began to increase at ischemia/reperfusion for 3 hours, then it gradually reached a peak level at ischemia/reperfusion for 1 day, and thereafter it gradually decreased. MMP-9 expression began to increase at ischemia/reperfusion for 3 hours, then gradually reached its peak level 2 days after perfusion, and th

关键词： ischemia/reperfusion injury matrix metalloproteinase-9 blood brain barrier

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

A Study on the Protection of Urban Fringe Habitat Units from the Perspective of the Actor-Network Theory

引用

China City Planning Review 2021年第3期30卷 6-18页

作者： Tang Xizi lei qiuyun Shenglin Elijah Chang Bi Linglan Zhang Yu Qian Fang(译) Liu Jian(校) School of Architecture and Design Southwest Jiaotong UniversityChengduP.R.China Graduate Institute Building&Planning National Taiwan UniversityTaipeiP.R.China 不详

Being an important part of a region’s ecological space,the habitat units on the urban fringe continuously provide multiple services for the city and its residents and guarantee the ecological security of the local *** composed of farmlands,secondary woodlands,ditches and ponds,etc.,they are typical semi-natural habitat which is subject to the influences of both the human society and the natural *** they involve multiple stakeholders and concern complex ownerships,top-down measures are difficult to be implemented,as presented by the Not-In-MyBackyard(NIMBY)phenomenon against protected *** on the Actor-Network Theory,this paper clarifies the intrinsic relationship between the maintenance of urban fringe habitat units and the relevant social *** the case study on Yilan’s practice of protecting and utilizing agricultural and forestry resources in Taiwan,China,the paper explores the community supported management and control strategies for the purposes of enhancing the protection of habitat units and promoting the effectiveness of regional services,which are implemented through the steps of implementing such translation methods as Obligatory Passage Point setting and benefit granting,recruiting and mobilizing relevant actors to form a network,and realizing the substantial protection of habitat units.

关键词： urban fringe habitat unit protection and utilization actor-network

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

城乡共享“自然-农业公园”——城乡结合部高价值小块农林用地保护规划方法研究

引用

城市规划 2021年第11期45卷 54-64页

作者：邢忠汤西子顾媛媛何依蔓雷秋云重庆大学建筑城规学院重庆大学山地城镇建设与新技术教育部重点实验室西南交通大学建筑与设计学院

交错区空间区位与用地属性赋予城乡结合部农林用地极高的城与乡共享价值。文章运用文献研究与案例比较的方法,探讨结合部内未划入基本农田与林地保护范围,但具有多样价值的分散小块农林斑块的规划路径。旨在通过自然-农业公园共享价值利... 详细信息

交错区空间区位与用地属性赋予城乡结合部农林用地极高的城与乡共享价值。文章运用文献研究与案例比较的方法,探讨结合部内未划入基本农田与林地保护范围,但具有多样价值的分散小块农林斑块的规划路径。旨在通过自然-农业公园共享价值利用,保护高价值农林用地,提升地域生态品质,达成食品物种多样性保护等复合目标。文章就此展开关联规划方法研究:制订"用地组织-功能保障-系统与指标控制-设计导引"的技术框架;梳理现状要素,立足地域空间生态过程选择公园用地并构筑自维持网络;依据环境特质与城乡共享价值赋予公园产业经济功能;外联乡村腹地自然绿斑绿廊,内引城区公共绿地,凸显与城乡地域空间互惠共享复合服务功能;硬、软件管控结合,落地规划目标。

关键词：城乡结合部城乡共享自然-农业公园高价值农林用地规划方法

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

非共价键构筑糖基表面活性剂及其性能研究

引用

化工时刊 2021年第4期35卷 5-11页

作者：雷秋云廖雄刘学民葛新江南大学化学与材料工程学院江苏无锡214122

利用N-十二烷基葡萄糖胺AGA12(和N-十二烷基乳糖胺ALA12)分别和柠檬酸以不同摩尔比通过非共价键构筑糖基表面活性剂(G-C-n和L-C-n)(n=1,2,3)。通过表面张力和电导率等手段,研究了单链烷基糖胺(AGA12、ALA12)与柠檬酸摩尔比和亲水基团对G... 详细信息

利用N-十二烷基葡萄糖胺AGA12(和N-十二烷基乳糖胺ALA12)分别和柠檬酸以不同摩尔比通过非共价键构筑糖基表面活性剂(G-C-n和L-C-n)(n=1,2,3)。通过表面张力和电导率等手段,研究了单链烷基糖胺(AGA12、ALA12)与柠檬酸摩尔比和亲水基团对G-C-n和L-C-n水溶液性能的影响。结果表明,随着单链烷基糖胺和柠檬酸摩尔比增加,糖基表面活性剂(G-C-n和L-C-n)的cmc值逐渐减小,pC20值逐渐增大,且这类表面活性剂水溶液的胶束化过程是热力学自发的熵驱动的放热过程。

关键词：非共价键构筑糖基表面活性剂溶液性能热力学性质

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Exceptional co-catalyst free photocatalytic activities of B and Fe co-doped SrTiO3 for CO2 conversion and H2 evolution

引用

Nano Research 2018年第12期11卷 6391-6404页

作者： Muhammad Humayun lei Xu Ling Zhou Zhiping Zheng qiuyun Fu Wei Luo Engineering Research Center for Functional Ceramics of the Ministry of Education School of Optical and Electronic InformationHuazhong University of Science and TechnologyWuhan 430074China

CO2 should be converted into chemical-fuels, and to reduce H2O to H2 over SrTiO3 (STO) owing to its negative conduction band position vs. NHE. Herein a novel B and Fe co-doped SrTiO3 (B, F-STO) photocatalyst was successfully fabricated via a single-step sol-hydrothermal process. Various experiments confirmed that B and Fe are effectively doped into the STO matrix. Boron substituted oxygen anions, while Fe substituted Ti cations. UV-visible diffuse reflectance spectra (UV-vis DRS) and valence-band X-ray photoelectron spectroscopy (XPS) spectra confirmed that the band gap of STO significantly reduced from 3.4 to 1.9 eV upon co-doping with B and Fe. Hence, the B, F-STO photocatalyst exhibits more absorption (λ ≤ 650 nm) compared to pure STO (λ ≤ 360 nm). Further, from photoluminescence spectra, fluorescence spectra, and photoelectrochemical measurements, charge separation in STO is considerably enhanced by co-doping B and Fe. This resulted in the improved UV-vis light catalytic activities for CO2 conversion to CH4 and CO and H2O splitting to evolve H2. The amounts of CH4 and CO produced over B, F-STO are ∼ 17.2 and 21 μmol, respectively, about 5-fold enhanced compared to that of STO (∼ 3.4 μmol CH4 and 5.2 μmol CO), and the calculated quantum efficiency at λ = 420 nm is ∼ 2.16%. Similarly, the amount of H2 produced over B, F-STO is ∼ 61 μmol, about 6.7-fold enhanced compared to that over STO (9 μmol), and the calculated quantum efficiency at λ = 420 nm is ∼ 2.12%. This work provides feasible routes to fabricate highly efficient SrTiO3-based nanophotocatalysts for solar-fuel production.

关键词： sol-hydrothermal process photocatalysis charge separation CO2 conversion H2 evolution

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

UPLC-MS metabolomics provides insights into the diff erences between black- and white-shelled Pacifi c oysters Crassostrea gigas

引用

Journal of Oceanology and Limnology 2021年第1期39卷 340-349页

作者： Xi CHEN qiuyun JIANG Hongce SONG Lingling LI Chaoyi XIE Baoyu HUANG Yaqiong LIU Meiwei ZHANG lei WEI Xiaotong WANG School of Agriculture Ludong UniversityYantai 264025China

A variety of shell colors are one of the most fundamental characteristics of molluscs,which have importantly ecological and economic signifi *** Pacifi c oyster Crassostrea gigas is distributed in many sea areas around the world and also an aquacultured mollusc with high nutritional *** this study,the whole soft body and the mantle tissue of black-shelled Pacifi c oyster(BSO)and white-shelled Pacifi c oyster(WSO)with starkly diff erent melanin contents were compared,and the diff erences in physiology and metabolism between BSO and WSO were *** results of physiological indicators suggested BSO show more melanin,more dry matter,more crude lipid content,and stronger ability to scavenge free radicals than *** altered metabolites of glycerophospholipids,fatty acyls,and steroids revealed diff erent regulatory mechanisms of *** correlation analysis of metabolomics and previously published RNAseq data suggested that BSO and WSO mainly diff ered in the basal metabolic processes,such as lipid,amino acid and purine *** study provides insights into the changes in the physiological indictors and the metabolites of oysters with varying melanin content.

关键词： Crassostrea gigas UPLC-MS metabolomics physiological indicators melanin shell color

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Electronic structure of LaIrIn5and f-electron character in its related Ce-115 compounds

引用

Science China(Physics,Mechanics & Astronomy) 2020年第11期63卷 90-97页

作者： Rui Zhou XueBing Luo ZhaoFeng Ding lei Shu XingYu Ji ZiHao Zhu YaoBo Huang DaWei Shen ZhengTai Liu ZhongHao Liu Yun Zhang qiuyun Chen Science and Technology on Surface Physics and Chemistry Laboratory Mianyang 621908China Department of Engineering Physics Tsinghua UniversityBeijing 100084China State Key Laboratory of Surface Physics and Department of Physics Fudan UniversityShanghai 200433China Shanghai Institute of Applied Physics Chinese Academy of SciencesShanghai 201204China Center of Excellence in Superconducting Electronics State Key Laboratory of Functional Materials for InformaticsShanghai Institute of Microsystem and Information TechnologyChinese Academy of SciencesShanghai 200050China

LaIrIn5 is a reference compound of the heavy-fermion superconductor *** lack of f electrons in LaIrIn5 indicates that there should not be any f electron participating in the construction of its Fermi *** the electronic structure comparison between LaIrIn5 and LaIrIn5 provides a good platform to study the properties of f *** angle-resolved photoemission spectroscopy(ARPES)measurements and density functional theory(DFT)calculations are performed to study the electronic structures of LaIrIn5 and *** find the valence band structures of the two materials are similar to each other,except for the absence of f bands in *** analyzing the Fermi crossings of the three conduction bands of the two materials quantitatively,we find the volumes of the electron pocketsαandβaround the M′point become larger from LaIrIn5 to LaIrIn5,while the hole pocketγaround theΓ′point becomes *** with the calculation results,we confirm that this is mainly originated from the f-electron contribution,while the lattice-constant difference between LaIrIn5 and LaIrIn5 only has a finite *** also give a summary of the f-electron character in its related Ce-115 heavy fermion *** results may be essential for the complete microscopic understanding of the 115 compounds and the related heavy-fermion systems.

关键词： ARPES DFT heavy fermion LaIrIn5

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

miR-429-3p mediates memory decline by targeting MKP-1 to reduce surface GluA1- containing AMPA receptors in a mouse model of Alzheimer’s disease

引用

Acta Pharmaceutica Sinica B 2024年第2期14卷 635-652页

作者： Man Luo Yayan Pang Junjie Li Lilin Yi Bin Wu qiuyun Tian Yan He Maoju Wang lei Xia Guiqiong He Weihong Song Yehong Du Zhifang Dong Pediatric Research Institute Ministry of Education Key Laboratory of Child Development and DisordersNational Clinical Research Center for Child Health and DisordersChina International Science and Technology Cooperation Base of Child Development and Critical DisordersChongqing Key Laboratory of Translational Medical Research in Cognitive Development and Learning and Memory DisordersChildren’s Hospital of Chongqing Medical UniversityChongqing 400014China Department of Anatomy Basic Medical CollegeChongqing Medical UniversityChongqing 400016China Townsend Family Laboratories Department of PsychiatryThe University of British ColumbiaVancouver BC V6T 1Z3Canada Oujiang Laboratory(Zhejiang Lab for Regenerative Medicine Vision and Brain Health)Institute of AgingKey Laboratory of Alzheimer’s Disease of Zhejiang ProvinceZhejiang Clinical Research Center for Mental DisordersSchool of Mental Health and the Affiliated Kangning HospitalWenzhou Medical UniversityWenzhou 325000China Institute for Brain Science and Disease of Chongqing Medical University Chongqing 400016China

Alzheimer ’s disease(AD) is a leading cause of dementia in the ***-activated protein kinase phosphatase 1(MKP-1) plays a neuroprotective role in ***,the molecular mechanisms underlying the effects of MKP-1 on AD have not been extensively ***(miRNAs) regulate gene expression at the post-transcriptional level,thereby repressing mRNA ***,we reported that the microRNA-429-3p(miR-429-3p) was significantly increased in the brain of APP23/PS45 AD model mice and N2AAPPAD model *** further found that miR-429-3p could downregulate MKP-1 expression by directly binding to its 3’-untranslated region(3’ UTR).Inhibition of miR-429-3p by its antagomir(A-miR-429) restored the expression of MKP-1 to a control level and consequently reduced the amyloidogenic processing of APP and Aβ *** importantly,intranasal administration of A-miR-429 successfully ameliorated the deficits of hippocampal CA1 long-term potentiation and spatial learning and memory in AD model mice by suppressing extracellular signal-regulated kinase(ERK1/2)-mediated GluAl hyperphosphorylation at Ser831 site,thereby increasing the surface expression of GluAl-containing α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptors(AMPARs).Together,these results demonstrate that inhibiting miR-429-3p to upregulate MKP-1 effectively improves cognitive and synaptic functions in AD model mice,suggesting that miR-429/MKP-1 pathway may be a novel therapeutic target for AD treatment.

关键词： Alzheimer's disease MKP-1 miR-429-3p AMPAreceptor Learning and memory Long-term potentiation

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

氧合酶家族分子在叶酸缺乏中对人神经母细胞瘤细胞、淀粉样前体蛋白和β位淀粉样前体裂解酶-1表达的影响

引用

中华老年医学杂志 2018年第3期37卷 324-329页

作者：雷艺丁斌荣唐旻涂秋云中南大学湘雅三医院老年科长沙410013

目的探讨氧合酶家族分子（TET）在叶酸缺乏条件下对人神经母细胞瘤细胞系SH-SY5Y、IMR-32细胞的增殖能力及淀粉样前体蛋白（APP）基因、早老素1（PS1）基因以及β位淀粉样前体裂解酶-1（BACE1）基因表达的影响及机制。方法体外培养SH-S... 详细信息

目的探讨氧合酶家族分子（TET）在叶酸缺乏条件下对人神经母细胞瘤细胞系SH-SY5Y、IMR-32细胞的增殖能力及淀粉样前体蛋白（APP）基因、早老素1（PS1）基因以及β位淀粉样前体裂解酶-1（BACE1）基因表达的影响及机制。方法体外培养SH-SY5Y、IMR-32细胞，分为叶酸缺乏组、低剂量组、正常对照组，采用噻唑蓝（MTT）法观察细胞增殖、实时荧光定量PCR（RT-PCR）检测细胞中APP、PS1、BACE1、TETs、甲基转移酶（DNMTs）基因mRNA的表达；构建TET1低表达稳转细胞株，采用荧光倒置显微镜观察荧光蛋白，MTT法观察细胞增殖，RT-PCR检测细胞中TET1、APP、PS1、BACE1的转录水平。结果（1）在叶酸缺乏组及低剂量组，培养120h和144h后sH-sY5Y、IMR-32细胞增殖能力明显降低（均P〈0．001）；SHSY5Y细胞中APP、PS1、BACE1、DNMT1、DNMT3a、DNMT3b、TET1、TET2、TET3的mRNA水平升高（F=80．315、35．386、101．979、786．407、80．331、131．545、28．000、9．165、102．167，均P〈0．05），IMR-32细胞中APP、PS1、BACE1、DNMT1、DNMT3b、TET1、TET2、TET3的mRNA水平升高（F=12．283、93．669、40．815、157．234、24．835、147．594、54．794、73．068，均P〈0．05）。（2）构建TET1低表达稳转细胞株，低表达组（SH-SY5Y-shTET1）细胞内TET1的相对表达量分别为0．25±0．02，低于阴性对照组1．00±0．09（P=0．007）；低表达组（IMR-32-shTET1）细胞内TET1的相对表达量分别为0．28±0．07，低于阴性对照组1．00±0．01（P=0．003）。（3）相较于阴性对照组，低表达组（SH-SY5Y-shTET1、IMR-32-shTET1）的增殖能力均增加（均P〈0．01）；且细胞内APP、BACE1的mRNA水平降低（P〈0．01或P〈0．05）。结论在人神经母细胞瘤细胞系SH-SY5Y、IMR-32中，叶酸缺乏上调TETs的表达，通过TET蛋白去甲基化途径增加细胞中APP、BACE1表达，并抑制细胞生长。

关键词：叶酸 DNA甲基转移酶氧合酶淀粉样蛋白 β位淀粉样前体裂解酶

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

复方高滋斑片对抑郁大鼠海马NLRP3炎症小体的影响

引用

中国医院药学杂志 2024年第18期44卷 2115-2121页

作者：肖敏韩正茹游秋云昝俊峰石雷尤朋涛湖北中医药大学药学院中药资源与中药化学省级重点实验湖北武汉430065

目的:研究复方高滋斑片(Compound Gaoziban Tablets,CGZBT)对抑郁大鼠的缓解作用和海马NLRP3炎症小体的潜在影响。方法:适应性喂养1周后根据0 weeks糖水偏好率将大鼠随机分为Control组、CUMS组、CGZBT-L组(0.4g·kg^(–1))、CGZBT-M组(0.8 g·kg^(–1))、CGZBT-H组(1.6 g·kg^(–1))、Fluoxetine组,每组12只。SD大鼠造模6周建立慢性不可预知轻度应激(CUMS)抑郁模型,从第5周开始在CUMS造模同时给予相应药物干预2周。最后1天对大鼠进行糖水偏好实验、旷场实验、强迫游泳实验。麻醉后对大鼠海马体、血清进行取材,苏木精-伊红染色法观察海马体CA1区神经元细胞的变化。用ELISA法检测血清中肿瘤坏死因子α(TNF-α)、白介素-1β(IL-1β)表达水平。Western blot法检测海马体中核苷酸结合寡聚化结构域样受体蛋白3(NLRP3)、凋亡相关斑点样蛋白(ASC)、半胱氨酸天冬氨酸蛋白酶-1(Caspase-1)、白细胞介素-1β前体(pro-IL-1β)、IL-1β、白介素-18(IL-18)的表达水平。结果:与CUMS组相比,CGZBT各剂量组旷场移动总距离显著增加(P<0.01),强迫游泳不动时间减少(P<0.01或P<0.05);CGZBT-M组和CGZBT-H组大鼠的糖水偏好率显著增加(P<0.01)。CGZBT可缓解CUMS大鼠海马体CA1区神经元细胞的损伤和降低血清中TNF-α、IL-1β的含量(P<0.01或P<0.05)。与CUMS组相比,CGZBT各剂量组大鼠海马中ASC、Caspase-1、IL-1β、的蛋白表达显著下调(P<0.01或P<0.05);CGZBT-M组和CGZBT-H组大鼠NLRP3、IL-18蛋白表达显著下调(P<0.01或P<0.05)。结论:CGZBT可缓解抑郁大鼠的抑郁症状并抑制海马体中NLRP3炎性小体的表达。

关键词：复方高滋斑片抑郁症炎症小体海马

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：