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Mechanism underlying berberine's effects on HSP70/TNFα under heat stress:Correlation with the TATA boxes

Chinese Journal of Natural Medicines 2017年第3期15卷 178-191页

作者： JIANG Jing-Fei lei fan YUAN Zhi-Yi WANG Yu-Gang WANG Xin-Pei YAN Xiao-Jin YU Xuan XING Dong-Ming DU Li-Jun MOE(Ministry of Education)Key Laboratory of Protein Sciences Laboratory of Molecular Pharmacology and Pharmaceutical SciencesSchool of Life SciencesTsinghua UniversityBeijing 100084China Department of Pharmacology and Pharmaceutical Sciences School of MedicineTsinghua UniversityBeijing 100084China MD Anderson Cancer Center University of TexasHoustonTX 77030USA

Heat stress can stimulate an increase in body temperature,which is correlated with increased expression of heat shock protein 70(HSP70) and tumor necrosis factor α(TNFα).The exact mechanism underlying the HSP70 and TNFα induction is unclear.Berberine(BBR) can significantly inhibit the temperature rise caused by heat stress,but the mechanism responsible for the BBR effect on HSP70 and TNFα signaling has not been investigated.The aim of the present study was to explore the relationship between the expression of HSP70 and TNFα and the effects of BBR under heat conditions,using in vivo and in vitro models.The expression levels of HSP70 and TNFα were determined using RT-PCR and Western blotting analyses.The results showed that the levels of HSP70 and TNFα were up-regulated under heat conditions(40 °C).HSP70 acted as a chaperone to maintain TNFα homeostasis with rising the temperature,but knockdown of HSP70 could not down-regulate the level of TNFα.Furthermore,TNFα could not influence the expression of HSP70 under normal and heat conditions.BBR targeted both HSP70 and TNFα by suppressing their gene transcription,thereby decreasing body temperature under heat conditions.In conclusion,BBR has a potential to be developed as a therapeutic strategy for suppressing the thermal effects in hot environments.

关键词： Berberine Hyperthermia HSP70 TNFα TATA box

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Role of baicalin in regulating Toll-like receptor 2/4 after ischemic neuronal injury

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Chinese Medical Journal 2012年第9期125卷 1586-1593页

作者： LI Hui-ying YUAN Zhi-yi WANG Yu-gang WAN Hong-jiao HU Jun CHAI Yu-shuang lei fan XING Dong-ming DU Li-jun Protein Science Laboratory of Ministry of Education Laboratory of Pharmaceutical Sciences Department of Biological Sciences and Biotechnology Tsinghua University Beijing 100084 China School of Basic Medicine Jiangxi University of Traditional Chinese Medicine Nanchang Jiangxi 330004 China

Background Baicalin has a significant anti-inflammation effect and is widely used in the clinical treatment of stroke. Most of the studies of Toll-like receptor 2/4 （TLR2/4） during cerebral ischemia had defined their specific expressions in microglia in hippocampus tissue. To explore the targets of baicalin in stroke, we detected the expressions of TLR2/4 in vitro/vivo.Methods By constructing a cerebral ischemia-reperfusion model in vivo and glucose oxygen deprivation model, we successfully induced neuron damage, then added baicalin and detected expressions of TLR2/4, nuclear factor-kB （NF-kB）, tumor necrosis factor-alpha （TNFα）, and interleukin-1β （IL-1β） in mRNA level and protein level.Results We found distinct upregulations of TLR2/4 and TNFα in both mRNA level and protein level in PC12 cells and primary neurons. Moreover, TLR2/4 and TNFα expressions were significantly higher in mice hippocampus treated with cerebral ischemia-reperfusion. Baicalin could downregulate the expressions of TLR2/4 and TNFα in the damaged cells and mice hippocampus effectively.Conclusions Neurons could respond to the damage and activate the related signal pathway directly. TLR2/4 responsed to the damage and sent the signal to downstream factor TNFα through activating NF-kB. Baicalin could inhibit the inflammatory reaction in neuron damage and TLR might be its targets, which explained why baicalin could widely be used in the clinical treatment of stroke.

关键词： Toll-like receptor 2/4 baicalin neuron oxygen glucose deprivation cerebral ischemia-reperfusion

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Thermal management for the underwater frontend and readout electronics of JUNO 20‑inch PMTs

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Radiation Detection Technology and Methods 2023年第3期7卷 418-426页

作者： Yangfu Wang Xiaoshan Jiang Jun Hu Peiliang Wang lei fan Shaojing Hou Zhe Ning Yunhua Sun Xiongbo Yan Zheng Wang Ziyue Yan Peiran Ye Jie Zhang Alberto Garfagnini Antonio Bergnoli Riccardo Brugnera Beatrice Jelmini Filippo Marini Marco Grassi Mariia Redchuk Andrea Serafini Katharina von Sturm Institute of High Energy Physics BeijingChina University of China Academy of Sciences Beijing 100049China INFN Sezione di Padova PaduaItaly Dipartimento di Fisica e Astronomia Universita di PadovaPaduaItaly

Background The Jiangmen underground neutrino observatory(JUNO)is a new generation of long-term operation neutrino experimental platform under construction in Jiangmen,Guangdong province of southern China.The underwater frontend and readout electronics(F&R electronics)are placed nearby the 20-inch PMTs and readout current signals from three PMTs.The electronics are sealed in a stainless-steel underwater box to keep them dry,and temperature control of the electronics plays a significant role in their reliability.Methods A conductive cooling structure based on the passive cooling method is designed for the 20-inch PMT F&R electronics.Both numerical simulation and experimental tests were performed to evaluate the cooling structures'thermal performance.Results The results show that the case temperature of the respective chip for 20-inch PMT F&R electronics is less than 30℃in ambient still water.Conclusion The case temperature of the respective chip meets the strict temperature requirement for the F&R electronics.The JUNO collaboration has adopted this cooling structure for the 20-inch PMT readout electronics.

关键词： Thermal management JUNO PMT Electronics

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Hybrid gelatin/oxidized chondroitin sulfate hydrogels incorporating bioactive glass nanoparticles with enhanced mechanical properties, mineralization, and osteogenic differentiation

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Bioactive Materials 2021年第3期6卷 890-904页

作者： lei Zhou lei fan Feng-Miao Zhang Yuhe Jiang Min Cai Cong Dai Yi-An Luo Ling-Jie Tu Zheng-Nan Zhou Xiao-Jun Li Cheng-Yun Ning Kai Zheng Aldo R.Boccaccini Guo-Xin Tan Institute of Chemical Engineering and Light Industry Guangdong University of TechnologyGuangzhou510006China Institute of Biomaterials Department of Materials Science and EngineeringUniversity of Erlangen-NurembergCauerstrasse 691058ErlangenGermany School of Materials Science and Engineering South China University of TechnologyGuangzhou510641China College of Arts and Sciences Stony Brook University100 Nicolls RoadStony BrookNY11794United States Department of Prosthodontics Hospital of StomatologyGuanghua School of StomatologySun Yat-sen UniversityGuangzhou510055China

Biopolymer based hydrogels are characteristic of their biocompatibility and capability of mimicking extracellular matrix structure to support cellular behavior.However,these hydrogels suffer from low mechanical properties,uncontrolled degradation,and insufficient osteogenic activity,which limits their applications in bone regeneration.In this study,we developed hybrid gelatin(Gel)/oxidized chondroitin sulfate(OCS)hydrogels that incorporated mesoporous bioactive glass nanoparticles(MBGNs)as bioactive fillers for bone regeneration.Gel-OCS hydrogels could be self-crosslinked in situ under physiological conditions in the presence of borax.The incorporation of MBGNs enhanced the crosslinking and accelerated the gelation.The gelation time decreased with increasing the concentration of MBGNs added.Incorporation of MBGNs in the hydrogels significantly improved the mechanical properties in terms of enhanced storage modulus and compressive strength.The injectability of the hydrogels was not significantly affected by the MBGN incorporation.Also,the proliferation and osteogenic differentiation of rat bone marrow mesenchymal stem cells in vitro and rat cranial defect restoration in vivo were significantly promoted by the hydrogels in the presence of MBGNs.The hybrid Gel-OCS/MBGN hydrogels show promising potential as injectable biomaterials or scaffolds for bone regeneration/repair applications given their tunable degradation and gelation behavior as well as favorable mechanical behavior and osteogenic activities.

关键词： Hydrogels Bioactive glasses Biopolymers Hybrids Bone regeneration

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“地下复合支撑”变形分析与优化设计

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地质与勘探 2024年第2期60卷 356-366页

作者：潘春宇易康朱锐付丽朱文卓严溪浩范磊周峰中建安装集团有限公司江苏南京210046 南京工业大学交通运输学院江苏南京211800

“地下复合支撑”是通过在基坑坑底布置加固水泥土将坑底工程桩与基坑支护桩墙相连形成复合受力体。基坑开挖时,复合受力体共同受力抵抗变形,有效控制围护结构变形并抑制坑底土体隆起。本文提出“地下复合支撑”概念及其设计理论,分析... 详细信息

“地下复合支撑”是通过在基坑坑底布置加固水泥土将坑底工程桩与基坑支护桩墙相连形成复合受力体。基坑开挖时,复合受力体共同受力抵抗变形,有效控制围护结构变形并抑制坑底土体隆起。本文提出“地下复合支撑”概念及其设计理论,分析坑底加固面积置换率、固化剂掺量、加固深度等关键设计参数对基坑变形的影响,为“地下复合支撑”投入工程实践提供设计依据和理论参考。基于工程实例,针对原支护体系给出地下复合支撑的优化设计方案,验证新型复合支撑的可行性,体现其对基坑围护结构水平位移和坑底隆起变形控制的优势。同时,按照优化设计方案可以减少基坑内支撑的道数,具有安全性和经济性。

关键词：深基坑坑底加固基坑支护数值模拟优化设计

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河南省森林固碳量与碳汇价值评估

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郑州大学学报（工学版） 2024年第3期45卷 7-13页

作者：于鲁冀张亚慧樊雷王莉刘莹莹郑州大学生态与环境学院河南郑州450001 河南省土地整理中心河南郑州450003 郑州大学河南省减污降碳协同工程技术研究中心河南郑州450001

为了解当前黄河中下游森林固碳量及碳汇价值现状,助力黄河流域生态保护和高质量发展,基于全国森林清查和国土调查数据,以河南省为例,结合蓄积量转换法原理运用改进后的生物量转换因子连续函数法来测算森林固碳量动态变化和碳汇价值。结... 详细信息

为了解当前黄河中下游森林固碳量及碳汇价值现状,助力黄河流域生态保护和高质量发展,基于全国森林清查和国土调查数据,以河南省为例,结合蓄积量转换法原理运用改进后的生物量转换因子连续函数法来测算森林固碳量动态变化和碳汇价值。结果表明:河南省森林固碳量由2008年的1.76×10^(8)t上升至2018年的2.78×10^(8)t,年固碳量较高的森林类型为阔叶混和栎类,固碳量按龄级分布状况为幼龄林最大、过熟林最小,森林固碳量的空间格局表现为“西、南高,东、北低”,主要分布在南阳、洛阳和三门峡;森林碳汇价值由2008年的492.47亿元增加至2018年的778.68亿元,年均增长28.62亿元,2018年阔叶混、栎类和杨树的碳汇价值占碳汇总价值的79.93%。针对当前河南省森林建设存在的问题,提出提升森林固碳能力和推动森林碳汇价值实现的建议,可为促进黄河流域高质量发展提供强劲动力。

关键词：森林固碳量碳密度碳汇价值蓄积量空间分布价值评估

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中药材VR智能展陈关键技术研究

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中国数字医学 2024年第3期19卷 73-78页

作者：张铁林范磊彭磊张恒振李延军山东中医药大学智能与信息工程学院济南250355 山东中医药大学中医文献与文化研究院济南250355

目的:利用VR智能展陈关键技术设计并实现中药材虚拟展示平台,实现中医药的网络化教育,探索中药材智能信息化的实现路径。方法:在3D建模技术为基础的条件下,使用C#语言,结合界面交互、文本文字等功能在Unity3D引擎中设计并实现中药材VR... 详细信息

目的:利用VR智能展陈关键技术设计并实现中药材虚拟展示平台,实现中医药的网络化教育,探索中药材智能信息化的实现路径。方法:在3D建模技术为基础的条件下,使用C#语言,结合界面交互、文本文字等功能在Unity3D引擎中设计并实现中药材VR智能展陈系统。结果:本系统实现了药材展示、场景漫游、碰撞检测、药材信息查询、切片模型转换等功能。结论:虚拟现实是中医药信息化实施路径的方式之一,此技术有利于中药材远程授课、提高教学效果及传播中医药文化。

关键词：虚拟现实关键技术中药材展厅

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葛仙米多糖提取物对模型体系中PhIP抑制效果研究

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中国调味品 2024年第6期49卷 9-14页

作者：于迪李优优姜东华孔繁磊皮亦华广西中医药大学药学院南宁530200 广西高发传染病中西医结合转化医学重点实验室南宁530200

高蛋白肉制品在热处理中容易生成稠环芳烃杂环胺,其中PhIP(2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine)是日常家庭烹饪中含量较高且有致癌风险的一种极性杂环胺。葛仙米为我国一种珍贵的野生蓝藻生物,是含有多种活性物质的一种... 详细信息

高蛋白肉制品在热处理中容易生成稠环芳烃杂环胺,其中PhIP(2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine)是日常家庭烹饪中含量较高且有致癌风险的一种极性杂环胺。葛仙米为我国一种珍贵的野生蓝藻生物,是含有多种活性物质的一种新食品原料。该实验在苯丙氨酸和肌酐为前体物质的模型体系中,分别加入0,30,50,100,150μg的葛仙米多糖提取物进行反应,利用UPLC-MS/MS对模型体系中反应前体物质苯丙氨酸和肌酐、中间产物苯乙醛及产物PhIP进行鉴定并监测含量变化,实验结果表明,葛仙米多糖提取物能较好地抑制杂环胺PhIP的生成,模型体系中苯丙氨酸、肌酐和苯乙醛含量均呈现先减少后增多的趋势。在葛仙米多糖提取物添加量为50μg时,对模型反应体系中PhIP的抑制效果最好,抑制率为84.9%。推测葛仙米多糖提取物作用机制是通过抑制苯丙氨酸降解生成苯乙醛,阻碍其与肌酐发生反应,最终抑制PhIP的生成。该研究为利用葛仙米多糖提取物抑制食品热加工中PhIP的生成奠定了理论基础,有利于进一步开发具有葛仙米特殊风味的功能性调味品,为调味品产业发展提供了新的思路。

关键词：葛仙米多糖提取物苯丙氨酸肌酐苯乙醛 PhIP 抑制机制

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Risk Factors and Pregnancy Outcomes： Complete versus Incomplete Placenta Previa in Mid-pregnancy

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Current Medical Science 2018年第4期38卷 597-601页

作者： Yun FENG Xue-yin LI Juan XIAO Wei LI Jing LIU Xue ZENG Xi CHEN Kai-yue CHEN lei fan Qing-ling KANG Su-hua CHEN Department of Obstetrics and Gynecology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 43000 China Department of Urology Zhengzhou First People's Hospital Zhengzhou 450000 China

This prospective study was conducted to compare risk factors and pregnancy outcomes between women with complete placenta previa and those with incomplete placenta previa diagnosed in mid-pregnancy. The study was carried out from April 2014 to December 2015, during which 70 patients with complete previa and 113 with incomplete previa between 20＋0 weeks and 25＋6 weeks of gestation were included. Maternal demographics and pregnancy outcomes were compared between the two groups. Comparisons between categorical variables were tested by chi-squared test and those between continuous variables by Student t test. Resolution ofprevia occurred in 87.43% of the studied women. The mean gestational age at resolution was 32.1＋4.4 weeks. Incidence of maternal age ≥35 years and incidence of prior uterine operation 〉3 were high in women with complete previa （28.6% vs. 8.8%, P=0.003; 28.6% vs. 8.8%, P=0.003）. Resolution ofprevia occurred less often in complete previa group （74.3% vs. 95.6%, P=0.001）. Women with complete previa admitted earlier （37.3±2.0 weeks vs. 38.1±1.4 weeks, P=0.011） and delivered earlier （37.7±1.2 weeks vs. 38.3±1.4 weeks, P=0.025）. Maternal age ≥35 years and prior uterine operation 〉3 increase the risk of complete previa in mid-pregnancy. Placenta previa is more likely to persist in women with complete previa than those with incomplete previa diagnosed in mid- pregnancy. What is more, women with complete previa in mid-pregnancy delivers earlier.

关键词： complete placenta previa risk factor uterine operation pregnancy outcome resolution

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Different techniques for harvesting grafts for living donor liver transplantation: A systematic review and meta-analysis

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World Journal of Gastroenterology 2017年第20期23卷 3730-3743页

作者： Hui Li Jun-Bin Zhang Xiao-Long Chen lei fan Li Wang Shi-Hui Li Qiao-Lan Zheng Xiao-Ming Wang Yang Yang Gui-Hua Chen Gen-Shu Wang Department of Hepatic Surgery and Liver Transplantation Center of the Third Affiliated Hospital Organ Transplantation InstituteSun Yat-Sen University Organ Transplantation Research Center of Guangdong Province Journal Publishing Center of The Third Affiliated Hospital Sun Yat-Sen University Department of Hepatobiliary Surgery Yijishan Hospital Affiliated to Wannan Medical College Guangdong Key Laboratory of Liver Disease Research the Third Affiliated Hospital of Sun Yat-Sen University

AIM To perform a systematic review and meta-analysis on minimally vs conventional invasive techniques for harvesting grafts for living donor liver transplantation.METHODS Pub Med,Web of Science,EMBASE,and the Cochrane Library were searched comprehensively for studies comparing MILDH with conventional living donor hepatectomy(CLDH).Intraoperative and postoperative outcomes(operative time,estimated blood loss,postoperative liver function,length of hospital stay,analgesia use,complications,and survival rate)were analyzed in donors and recipients.Articles were included if they:(1)compared the outcomes of MILDH and CLDH;and(2)reported at least some of the above outcomes.RESULTS Of 937 articles identified,13,containing 1592 patients,met our inclusion criteria and were included in the meta-analysis.For donors,operative time[weighted mean difference(WMD)=20.68,95%CI:-6.25-47.60, =0.13]and blood loss(WMD=-32.61,95%CI:-80.44-5.21, =0.18)were comparable in the two groups.In contrast,analgesia use(WMD=-7.79,9 5%C I:-1 4.0 6-1.8 7, =0.0 1),posto pera tive complications[odds ratio(OR)=0.62,95%CI:0.44-0.89, =0.009],and length of hospital stay(W M D):-1.2 5,9 5%C I:-2.3 5-0.1 4, =0.0 3)significantly favored MILDH.No differences were observed in recipient outcomes,including postoperative complications(OR=0.93,95%CI:0.66-1.31, =0.68)and survival rate(HR=0.96,95%CI:0.27-3.47, =0.95).Funnel plot and statistical methods showed a low probability of publication bias.CONCLUSION MILDH is safe,effective,and feasible for living donor liver resection with fewer donor postoperative complications,reduced length of hospital stay and analgesia requirement than CLDH.

关键词：生活施主 hepatectomy 收获的接枝最低限度地侵略的技术常规侵略途径元分析

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