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Single Particle Colorimetric Acid Phosphatase Activity Assay with CeO_(2)-modified Gold Nanoparticles

Chemical Research in Chinese Universities 2024年第2期40卷 320-325页

作者： ZHU Tang Jiayao YUAN Xiang MAO Guojiang WEI Lin xiao lehui Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research National and Local Joint Engineering Laboratory for New Petrochemical Materials and Fine Utilization of ResourcesCollege of Chemistry and Chemical EngineeringHunan Normal UniversityChangsha410081P.R.China Henan Key Laboratory of Organic Functional Molecule and Drug Innovation Collaborative Innovation Center of Henan Province for Green Manufacturing of Fine ChemicalsKey Laboratory of Green Chemical Media and ReactionsMinistry of EducationSchool of Chemistry and Chemical EngineeringHenan Normal UniversityXinxiang453007P.R.China College of Chemistry and Chemical Engineering Central South UniversityChangsha410083P.R.China

Acid phosphatase(ACP)is a ubiquitous phosphatase in living organisms.The abnormal variation of ACP is related to various diseases.Herein,we propose a colorimetric method based on CeO_(2)-modified gold core shell nanoparticles(Au@CeO_(2)NPs)to analyze ACP activity with high sensitivity and specificity.In this design,2-phospho-L-ascorbic acid trisodium salt(AAP)is dephosphorylated by ACP and produces reductive ascorbic acid(AA),which makes the CeO_(2)shell decomposition.A remarkable blue shift of localized surface plasmon resonance peak(LSPR,from yellow to green)along with the scattering intensity ratio changes from individual Au@CeO_(2)NPs are observed.ACP activity can be quantified by calculating the ratio changes of individual Au@CeO_(2)NPs.This assay reveals limit of detection(LOD)of 0.044 mU/mL and the linear range of 0.05–5.0 mU/mL,which are much lower than most of spectroscopic measurements in bulk solution.Furthermore,the recovery measurements in real samples are satisfactory and the capacity for practical application is demonstrated.As a consequence,Au@CeO_(2)NPs used in this assay will find new applications for the ultrasensitive detection of enzyme activity.

关键词： Single particle imaging Dark field Gold nanoparticle Enzyme activity Colorimetric detection

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Curved carbon photo-oxygenation catalysts for the suppression and nanoscopic imaging ofβ-amyloid peptides fibrillation

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Nano Research 2022年第4期15卷 3387-3397页

作者： Yuanyuan Ma Zhongju Ye Chen Zhang Yanjun Zhao Hai-bin Luo lehui xiao College of Chemistry Zhengzhou UniversityZhengzhou 450001China State Key Laboratory of Medicinal Chemical Biology Tianjin Key Laboratory of Biosensing and Molecular RecognitionCollege of ChemistryNankai UniversityTianjin 300071China School of Pharmaceutical Sciences Sun Yat-sen UniversityGuangzhou 510006China School of Pharmaceutical Science&Technology Tianjin UniversityTianjin 300072China

The progression of Alzheimer’s disease(AD)is characterized with the deposition and aggregation ofβ-amyloid(Aβ).Visualizing Aβaggregates at high spatial resolution is beneficial for AD diagnosis and treatment.Herein,we designed a new molecule by conjugating corannulene(Cor)with rhodamine B isothiocyanate(Rhb),namely Cor-Rhb,for the nanoscopic imaging and modulating Aβpeptide fibrillation.The low duty cycle,high photon output and sufficient switching cycles enable Cor-Rhb suitable for localization-based nanoscopic fluorescence imaging.We find that Cor-Rhb can inhibit Aβpeptides fibrillization and interact directly with mature fibrils,triggering their disaggregation under light illumination.Noticeably reduced Aβ-mediated cytotoxicity after the addition of Cor-Rhb is also confirmed.These explorations suggest that Cor-Rhb displays great potential as a multifunctional therapeutic agent against amyloid-related diseases,and may largely facilitate a variety of super-resolution based biological applications.

关键词： nanoscopic imaging β-amyloid reactive oxygen species fluorescence imaging fibrillation

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Dissection the endocytic routes of viral capsid proteins-coated upconversion nanoparticles by single-particle tracking

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Chinese Chemical Letters 2022年第10期33卷 4710-4714页

作者： Yujun Ning Lin Wei Shen Lin Yifan Jiang Naidong Wang lehui xiao College of Chemistry Zhengzhou UniversityZhengzhou 450001China State Key Laboratory of Medicinal Chemical Biology Tianjin Key Laboratory of Biosensing and Molecular RecognitionCollege of ChemistryNankai UniversityTianjin 300071China College of Chemistry and Chemical Engineering Hunan Normal UniversityChangsha 410081China College of Veterinary Medicine Hunan Agricultural UniversityChangsha 410128China School of Chemistry and Chemical Engineering Henan Normal UniversityXinxiang 453007China

Real-time exploring the cellular endocytic pathway of viral capsid proteins(VCPs)functionalized nanocargos at the single-particle level can provide deep insight into the kinetic information involved in virus infection.In this work,porcine circovirus type 2(PCV2)VCPs with different functions are modified onto the surface of upconversion nanoparticles(VCPs-UCNPs)to investigate the cellular internalization process in real-time.Clathrin-mediated endocytosis is found to be the essential uptake mechanism for these VCPs-UCNPs.Besides,it is verified that P_(1)-UCNPs(PCV2 VCPs with nuclear localization signal,namely P1)can be easily assembled close to the perinuclear area,which is different from that of P_(2)-UCNPs(PCV2 VCPs without nuclear localization signal,namely P_(2)).Interestingly,multistep entry processes are observed.Particularly,confined diffusion is observed during the transmembrane process.The intracellular transport of VCPs-UCNPs is dependent on microtubules toward the cell interior.During this process,P_(1)-UCNPs display increased velocities with active transport,while diffusion much faster around the perinuclear area.But for P_(2)-UCNPs,there are only two phases involved in their endocytosis process.This study presents distinct dynamic mechanisms for the nanocargos with different functions,which would make a useful contribution to the development of robust drug delivery systems.

关键词： Upconversion nanoparticles Viral capsid proteins Single-particle tracking Hepatoma cells Endocytosis

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Fast Surface Restructuring within the Gap of Au Nanocube Dimer for the Enhancement of Catalytic Efficiency

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CCS Chemistry 2022年第3期4卷 1074-1086页

作者： xiao Wang Zhongju Ye Jianhao Hua Lin Wei Shen Lin lehui xiao State Key Laboratory of Medicinal Chemical Biology Tianjin Key Laboratory of Biosensing and Molecular RecognitionCollege of ChemistryNankai UniversityTianjin 300071 College of Chemistry and Chemical Engineering Hunan Normal UniversityChangsha 410081

Identification of the catalytic dynamics and plasmonic effects plays a critical role in the design of heterogeneous catalysts.However,the knowledge of plasmonic effect on catalytic dynamics remains limited at the single-particle level.Using the non-fluorescent amplex red to fluorescent resorufin as a model reaction,significant enhancement in catalytic efficiency from the coupled Au nanocube dimer(AuCD)was clearly revealed with the single-molecule fluorescence microscopy.AuCD exhibits noticeably higher catalytic efficiency than the monomer,which is attributed to the spontaneous dynamic surface restructuring.Spatiotemporally resolved dynamics suggest that the active catalytic sites essentially originate from the plasmonic nanogap where an electromagnetic(EM)hot spot exists.The enhanced EM field accelerates the generation of hot carriers and promotes the spontaneous surface restructuring by enhancing the lattice vibrations,which ultimately improves the catalytic activity.These microscopic views provide new insights into the effect of EM fields on surface restructuring dynamics of nanocatalysts.

关键词： dark-field plasmonic nanoparticles Raman spectroscopy surface dynamic restructuring single-particle imaging

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A Carbonized Fluorescent Nucleolus Probe Discloses RNA Reduction in the Process of Mitophagy

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CCS Chemistry 2022年第8期4卷 2698-2710页

作者： Hua Liu Xin Geng Xin Wang Lin Wei Zhaohui Li Shen Lin lehui xiao College of Chemistry Zhengzhou UniversityZhengzhou 450001 State Key Laboratory of Medicinal Chemical Biology Tianjin Key Laboratory of Biosensing and Molecular RecognitionCollege of ChemistryNankai UniversityTianjin 300071 College of Chemistry and Chemical Engineering Hunan Normal UniversityChangsha 410081

Mitophagy is a complicated process of cell metabolism that exhibits dynamic spatiotemporal coordination between multiple organelles.Despite its relevance to nucleoli,visualizing the distribution of nucleolar RNA in th... 详细信息

关键词： mitophagy RNA nanoscopic imaging carbon dots blinking

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Nanozyme-Triggered Cascade Reactions from Cup-Shaped Nanomotors Promote Active Cellular Targeting

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Research 2022年第3期2022卷 117-131页

作者： Xin Wang Zhongju Ye Shen Lin Lin Wei lehui xiao State Key Laboratory of Medicinal Chemical Biology Tianjin Key Laboratory of Biosensing and Molecular RecognitionCollege of ChemistryNankai UniversityTianjin 300071China College of Chemistry Zhengzhou UniversityZhengzhou 450001China Key Laboratory of Chemical Biology&Traditional Chinese Medicine Research Key Laboratory of Phytochemical R&D of Hunan ProvinceCollege of Chemistry and Chemical EngineeringHunan Normal UniversityChangsha 410082China

Self-propelled nanomotors have shown enormous potential in biomedical applications.Herein,we report on a nanozymepowered cup-shaped nanomotor for active cellular targeting and synergistic photodynamic/thermal therapy under nearinfrared(NIR)laser irradiation.The nanomotor is constructed by the asymmetric decoration of platinum nanoparticles(PtNPs)at the bottom of gold nanocups(GNCs).PtNPs with robust peroxidase-(POD-)like activity are employed not only as propelling elements for nanomotors but also as continuous O_(2)generators to promote photodynamic therapy via catalyzing endogenous H_(2)O_(2)decomposition.Owing to the Janus structure,asymmetric propulsion force is generated to trigger the shortranged directional diffusion,facilitating broader diffusion areas and more efficient cellular searching and uptake.This cascade strategy combines key capabilities,i.e.,endogenous substrate-based self-propulsion,active cellular targeting,and enhanced dual-modal therapy,in one multifunctional nanomotor,which is crucial in advancing self-propelled nanomotors towards eventual therapeutic agents.

关键词： endogenous shaped Active

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Blinking CsPbBr_(3) perovskite nanocrystals for the nanoscopic imaging of electrospun nanofibers

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Nano Research 2021年第5期14卷 1397-1404页

作者： Tianyu Chen Mengna Huang Zhongju Ye Jianhao Hua Shen Lin Lin Wei lehui xiao State Key Laboratory of Medicinal Chemical Biology Tianjin Key Laboratory of Biosensing and Molecular RecognitionCollege of ChemistryNankai UniversityTianjin300071China Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research Ministry of EducationKey Laboratory of Phytochemical R&D of Hunan ProvinceCollege of Chemistry and Chemical EngineeringHunan Normal UniversityChangsha410081China

Blinking fluorophore perovskite nanocrystals (NCs) were employed to image the fine structure of the polystyrene (PS) electrospun fibers. The conditions of CsPbBr3 NCs embedded and dispersed into PS were investigated and optimized. The stochastic optical reconstruction microscopy is employed to visualize the fine structure of the resulted CsPbBr3@PS electrospun fibers at sub-diffraction limit. The determined resolution in the reconstructed nanoscopic image is around 25.5 nm, which is much narrower than that of conventional fluorescence image. The complex reticulation and multicompartment in bead sub-diffraction-limited structures of CsPbBr3@PS electrospun fibers were successfully mapped with the help of the stochastic blinking properties of CsPbBr3 NCs. This work demonstrated the potential applications of CsPbBr3 perovskite NCs in super-resolution fluorescence imaging to reconstruct the sub-diffraction-limited features of polymeric material.

关键词： CsPbBr_(3) perovskite nanocrystals polystyrene electrospun nanofiber super-resolution optical imaging

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闪光放射治疗(Flash-RT)在肿瘤治疗中的研究进展

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中国医学装备 2024年第1期21卷 9-14,20页

作者：王源杜乐辉张沛商庆超郭兴东卢江岳雷霄曲宝林解放军总医院第一医学中心放射治疗科北京100853

闪光放射治疗(Flash-RT)作为放射治疗技术基础领域的关键性突破,可能引起放疗领域新的大变革。本文综述了Flash-RT在肿瘤治疗中应用和机制探索的最新研究进展。目前研究发现无论是电子束和光子Flash-RT还是质子FlashRT相较于常规剂量率... 详细信息

闪光放射治疗(Flash-RT)作为放射治疗技术基础领域的关键性突破,可能引起放疗领域新的大变革。本文综述了Flash-RT在肿瘤治疗中应用和机制探索的最新研究进展。目前研究发现无论是电子束和光子Flash-RT还是质子FlashRT相较于常规剂量率放疗均可以降低对正常组织的损伤,但相关机制还未明确,包括但不限于氧耗竭、DNA损伤、细胞衰老、凋亡和免疫反应等。Flash-RT在肿瘤组织与正常组织损伤间的差异进一步减少了放疗的局限性,相较于常规放疗减少了不良反应和并发症,具有广阔应用前景。

关键词：闪光放射治疗(Flash-RT) 超高剂量率肿瘤治疗机制

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单分子荧光成像研究单颗粒纳米催化机制

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化学学报 2023年第8期81卷 1002-1014页

作者：王晓王星文肖乐辉南开大学化学学院天津300071 中南大学化学化工学院长沙410083

纳米颗粒通常具有优异的催化性能,但由于其内在的异质性,宏观水平的表征难以确定单个纳米颗粒可靠的构效关系和潜在的催化反应机制.单分子荧光成像技术具有单分子灵敏度、高时空分辨率的优点,可以在单颗粒水平实现反应产物的超灵敏检测... 详细信息

纳米颗粒通常具有优异的催化性能,但由于其内在的异质性,宏观水平的表征难以确定单个纳米颗粒可靠的构效关系和潜在的催化反应机制.单分子荧光成像技术具有单分子灵敏度、高时空分辨率的优点,可以在单颗粒水平实现反应产物的超灵敏检测,因而在纳米催化领域得到了广泛应用.本文综述了单分子荧光成像的发展以及该技术在揭示单颗粒纳米催化反应机制中的应用,主要包括尺寸效应、晶面效应、表面缺陷、等离激元效应、双金属效应、活化能、纳米限域效应以及单颗粒催化通讯等方面.最后总结和展望了单分子荧光成像技术在纳米催化研究中的挑战与发展方向.

关键词：单分子荧光单颗粒时空分辨率纳米催化机制构效关系

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闪光放射治疗(Flash-RT)技术的研究进展

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中国医学装备 2024年第1期21卷 2-8页

作者：戴相昆吴韶鹃王金媛俞伟杜乐辉阎长鑫张石磊马娜雷霄曲宝林解放军总医院第一医学中心放射治疗科北京100853

经过多年发展,精准放射治疗技术已广泛应用,但现有技术仍受限于正常组织耐受剂量的限制,无法实现肿瘤治疗的最佳目标。闪光放射治疗(Flash-RT)是一种以超高剂量率射束(UHDR)进行照射的放射治疗技术,能够在显著降低正常组织辐射损伤的同... 详细信息

经过多年发展,精准放射治疗技术已广泛应用,但现有技术仍受限于正常组织耐受剂量的限制,无法实现肿瘤治疗的最佳目标。闪光放射治疗(Flash-RT)是一种以超高剂量率射束(UHDR)进行照射的放射治疗技术,能够在显著降低正常组织辐射损伤的同时,最大限度地治疗肿瘤。但直到目前,Flash-RT的生物学机制、关键物理参数及触发机制等尚不明确,其原理及临床转化应用仍处于研究阶段。本综述通过归纳Flash-RT相关研究,阐明Flash-RT研究的技术进展及临床转化应用。

关键词：放射治疗超高剂量率闪光放射治疗(Flash-RT)

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