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Coronatine promotes maize water uptake by directly binding to the aquaporin ZmPIP2;5 and enhancing its activity

Journal of Integrative Plant Biology 2023年第3期65卷 703-720页

作者： Rui He Huiqing Su Xing Wang Zhijie Ren Kun Zhang Tianyu Feng Mingcai Zhang Zhaohu li legong li Junhong Zhuang Zhizhong Gong Yuyi Zhou liusheng Duan State Key Laboratory of Plant Physiology and Biochemistry Engineering Research Center of Plant Growth RegulatorMinistry of Education&College of Agronomy and BiotechnologyChina Agricultural UniversityBeijing 100193China College of Plant Protection China Agricultural UniversityBeiing 100193China Key Laboratory of Plant Gene Resources and Biotechnology for Carbon Reduction and Environmental Improvement Beijing Municipal GovernmentCollege of Life SciencesCapital Normal UniversityBeijing 100048China Center for Crop Functional Genomics and Molecular Breeding China Agricultural UniversityBeijing 100193China State Key Laboratory of Plant Physiology and Biochemistry College of Biological SciencesChina Agricultural UniversityBeijing 100193China

Water uptake is crucial for crop growth and development and drought stress tolerance. The water channel aquaporins(AQP) play important roles in plant water uptake. Here, we discovered that a jasmonic acid analog, coronatine(COR), enhanced maize(Zea mays) root water uptake capacity under artificial water deficiency conditions. COR treatment induced the expression of the AQP gene Plasma membrane intrinsic protein 2;5(ZmPIP2;5).In vivo and in vitro experiments indicated that COR also directly acts on ZmPIP2;5 to improve water uptake in maize and Xenopus oocytes. The leaf water potential and hydraulic conductivity of roots growing under hyperosmotic conditions were higher in ZmPIP2;5-overexpression lines and lower in the zmpip2;5 knockout mutant, compared to wild-type plants. Based on a comparison between ZmPIP2;5 and other PIP2s, we predicted that COR may bind to the functional site in loop E of ZmPIP2;5. We confirmed this prediction by surface plasmon resonance technology and a microscale thermophoresis assay, and showed that deleting the binding motif greatly reduced COR binding. We identified the N241 residue as the COR-specific binding site, which may activate the channel of the AQP tetramer and increase water transport activity,which may facilitate water uptake under hyperosmotic stress.

关键词： coronatine osmotic stress water uptake capacity ZmPIP2 5

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A DTX/MATE-Type Transporter Facilitates Abscisic Acid Efflux and Modulates ABA Sensitivity and Drought Tolerance in Arabidopsis

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Molecular Plant 2014年第10期7卷 1522-1532页

作者： Haiwen Zhang Huifen Zhu Yajun Pan Yuexuan Yu Sheng Luan legong li College of life Sciences Capital Normal University Beijing 100048 China Department of Plant and Microbial Biology University of California Berkeley CA 94720 USA Institute of Plant Molecular Biology School of Life Sciences Nanjing University Nanjing 210093 China

Abscisic acid （ABA） regulates numerous physiological and developmental processes in plants. Recent studies identify intracellular ABA receptors, implicating the transport of ABA across cell membranes as crucial for ABA sensing and response. Here, we report that a DTX/Multidrug and Toxic Compound Extrusion （MATE） family member in Arabidopsis thaliana, AtDTX50, functions as an ABA efflux transporter. When expressed heterologously in both an Escherichia coli strain and Xenopus oocyte cells, AtDTX50 was found to facilitate ABA efflux. Furthermore, dtx50 mutant mesophyll cells preloaded with ABA released less ABA compared with the wild-type （WT）. The AtDTX50 gene was expressed mainly in the vascular tissues and guard ceils and its expression was strongly up-regulated by exogenous ABA. The AtDTX50：：GFP fusion protein was localized predominantly to the plasma membrane. The dtx50 mutant plants were observed to be more sensitive to ABA in growth inhibition. In addition, compared with the WT, dtx50 mutant plants were more tolerant to drought with lower stomatal conductance, consistent with its function as an ABA efflux carrier in guard cells.

关键词： hormone biology molecular transport Arabidopsis.

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Arabidopsis PRK6 interacts specifically with AtRopGEF8/12 and induces depolarized growth of pollen tubes when overexpressed

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Science China(life Sciences) 2018年第1期61卷 100-112页

作者： Yuexuan Yu Jiali Song Xiaohui Tian Haiwen Zhang legong li Huifen Zhu College of life Sciences Capital Normal University

The pollen receptor kinases (PRK) are critical regulators of pollen tube growth. The Arabidopsis genome encodes eight PRK genes, of which six are highly expressed in pollen tubes. The potential functions of AtPRK1 through AtPRK5, but not of AtPRK6,in pollen growth were analyzed in tobacco. Herein, AtPRK6 was cloned, and its function was identified. AtPRK6 was expressed specifically in pollen tubes. A yeast two-hybrid screen of AtPRK6 against 14 Arabidopsis Rop guanine nucleotide exchange factors (RopGEFs) showed that AtPRK6 interacted with AtRopGEF8 and AtRopGEF12. These interactions were confirmed in Arabidopsis mesophyll protoplasts. The interactions between AtPRK6 and AtRopGEF8/12 were mediated by the C-termini of AtRopGEF8/12 and by the juxtamembrane and kinase domain of AtPRK6, but were not dependent on the kinase activity. In addition, transient overexpression of AtPRK6::GFP in Arabidopsis protoplasts revealed that AtPRK6 was localized to the plasma membrane. Tobacco pollen tubes overexpressing AtPRK6 exhibited shorter tubes with enlarged tips. This depolarized tube growth required the kinase domain of AtPRK6 and was not dependent on kinase activity. Taken together, the results show that AtPRK6,through its juxtamembrane and kinase domains (KD), interacts with AtRopGEF8/12 and plays crucial roles in polarized growth of pollen tubes.

关键词： Arabidopsis 试管花粉生长交往相互作用染色体编码屏幕显示

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The SNW Domain of SKIP Is Required for Its Integration into the Spliceosome and Its Interaction with the Pall Complex in Arabidopsis

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Molecular Plant 2016年第7期9卷 1040-1050页

作者： Yan li Con qconcl Xia Jinlin Feng Dong Yang Fangming Wu Ying Cao legong li ligeng Ma College of life Sciences Hebei Normal University Shijiazhuang Hebei 050021 China Key Laboratory of Plant Gene Resources and Biotechnology for Carbon Reduction and Environmental Improvement Beijing Municipal GovernmentBeijing 100048 China College of life Sciences Capital Normal University Beijing 100048 China

SKIP is a conserved protein from yeasts to plants and humans. In plant cells, SKIP is a bifunctional regulator that works in the nucleus as a splicing factor by integrating into the spliceosome and as a transcriptional activator by interacting with the Pall complex. In this study, we identified two nuclear localization signals in SKIP and confirmed that each is sufficient to target SKIP to the nucleus. The SNW domain of SKIP is required for both its function as a splicing factor by promoting integration into the spliceosome in response to stress, and its function as a transcriptional activator by controlling its interaction with the Pall complex to participate in flowering. Truncated proteins that included the SNW domain and the N- or C-terminus of SKIP were still able to carry out the functions of the full-length protein in gene splicing and transcriptional activation in Arabidopsis. In addition, we found that SKIP undergoes 26S proteasome-mediated degrada- tion, and that the C-terminus of SKIP is required to maintain the stability of the protein in plant cells. Together, our findings demonstrate the structural domain organization of SKIP and reveal the core domains and motifs underlying SKIP function in plants.

关键词： SKIP spliceosome Pafl complex core domain and motif nuclear localization signal 26Sproteasome-mediated degradation

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Arabidopsis CNGC14 Mediates Calcium Influx Required for Tip Growth in Root Hairs

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Molecular Plant 2017年第7期10卷 1004-1006页

作者： Sisi Zhang Yajun Pan Wang Tian Mengqi Dong Huifen Zhu Sheng Luan legong li College of life Sciences Capital Normal University Key Laboratory of Plant Gene Resources and Biotechnology for Carbon Reduction and Environmental Improvement Beijing Municipal Government Beijing 100048 China Department of Plant and Microbial Biology University of California Berkeley CA 94720 USA

Dear Editor, Root hairs create an enormous surface area to aid plants in nutrient and water acquisition, anchorage in the soil, and microbe interactions. Root hairs develop in two stages, including initiation and tip growth. Initiation occurs when a portion of genetically determined epidermal cells develop wall loosening and form a disc-shaped area of cell swelling.

关键词： NUCLEOTIDE-GATED CHANNEL POLLEN GRAVITROPISM THAliANA GUIDANCE

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CIPK9: a calcium sensor-interacting protein kinase required for low-potassium tolerance in Arabidopsis

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Cell Research 2007年第5期17卷 411-421页

作者： Girdhar K Pandey Yong Hwa Cheong Beom-Gi Kim John J Grant legong li Department of Plant and Microbial Biology University of California Berkeley CA 94720 USA Department of Plant and Microbial Biology University of California

Potassium is one of the major macro-nutrients essential for a number of cellular processes in plants. Low potassiumlevel in the soil represents a limiting factor for crop production. Recent studies have identified potassium transporters thatare involved in potassium acquisition, and some of them are critical for potassium nutrition under low potassium condi-tions. However, little is understood on the molecular components involved in low potassium signaling and responses. Wereport here the identification of a calcineurin B-like protein-interacting protein kinase (CIPK9) as a critical regulator oflow potassium response in Arabidopsis. The CIPK9 gene was responsive to abiotic stress conditions, and its transcriptwas inducible in both roots and shoots by potassium deprivation. Disruption of CIPK9 function rendered the mutantplants hypersensitive to low potassium media. Further analysis indicated that K~+ uptake and content were not affected inthe mutant plants, implying CIPK9 in the regulation of potassium utilization or sensing processes.

关键词： calcium calcineurin-B like protein protein kinase potassium nutrition signal transduction

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The interaction of CaM7 and CNGC14 regulates root hair growth in Arabidopsis

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Journal of Integrative Plant Biology 2020年第7期62卷 887-896页

作者： Qudsia Zeb Xiaohan Wang Congcong Hou Xiwen Zhang Mengqi Dong Sisi Zhang Qian Zhang Zhijie Ren Wang Tian Huifen Zhu legong li liangyu liu Key Laboratory of Plant Gene Resources and Biotechnology for Carbon Reduction and Environmental Improvement Beijing Municipal Governmentand College of Life SciencesCapital Normal UniversityBeijing 100048China

Oscillations in cytosolic free calcium determine the polarity of tip-growing root hairs.The Ca2+channel cyclic nucleotide gated channel 14(CNGC14)contributes to the dynamic changes in Ca^2+concentration gradient at the root hair tip.However,the mechanisms that regulate CNGC14 are unknown.In this study,we detected a direct interaction between calmodulin 7(CaM7)and CNGC14 through yeast two-hybrid and bimolecular fluorescence complementation assays.We demonstrated that the third EF-hand domain of CaM7 specifically interacts with the cytosolic C-terminal domain of CNGC14.A two-electrode voltage clamp assay showed that CaM7 completely inhibitsCNGC14-mediated Ca2+influx,suggesting that CaM7 neg-atively regulates CNGC14-mediated calcium signaling.Fur-thermore,CaM7 overexpressing lines phenocopy the short root hair phenotype of a cngc14 mutant and this phenotype is insensitive to changes in external Ca^2+concentrations.We,thus,identified CaM7-CNGC14 as a novel interacting module that regulates polar growth in root hairs by con-trolling the tip-focused Ca2+signal.

关键词： expressing interaction CNG

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A chloride efflux transporter,BIG RICE GRAIN 1,is involved in mediating grain size and salt tolerance in rice

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Journal of Integrative Plant Biology 2021年第12期63卷 2150-2163页

作者： Zhijie Ren Fenglin Bai Jingwen Xu li Wang Xiaohan Wang Qian Zhang Changxin Feng Qi Niu liying Zhang Jiali Song Fang Bao liangyu liu Yikun He ligeng Ma Wang Tian Congcong Hou legong li College of life Sciences Capital Normal Universityand Beijing Key Laboratory of Plant Gene Resources and Biotechnology for Carbon Reduction and Environmental ImprovementBeijing 100048China School of Advanced Agricultural Sciences Peking UniversityBeijing 100871China

Grain size is determined by the size and number of cells in the grain.The regulation of grain size is crucial for improving crop yield;however,the genes and molecular mechanisms that control grain size remain elusive.Here,we report that a member of the detoxification efflux carrier/Multidrug and Toxic Compound Extrusion(DTX/MATE)family transporters,BIG RICE GRAIN 1(BIRG1),negatively influences grain size in rice(Oryza sativa L.).BIRG1 is highly expressed in reproductive organs and roots.In birg1 grain,the outer parenchyma layer cells of spikelet hulls are larger than in wild-type(WT)grains,but the cell number is unaltered.When expressed in Xenopus laevis oocytes,BIRG1 exhibits chloride efflux activity.Consistent with this role of BIRG1,the birg1 mutant shows reduced tolerance to salt stress at a toxic chloride level.Moreover,grains from birg1 plants contain a higher level of chloride than those of WT plants when grown under normal paddy field conditions,and the roots of birg1 accumulate more chloride than those of WT under saline conditions.Collectively,the data suggest that BIRG1 in rice functions as a chloride efflux transporter that is involved in mediating grain size and salt tolerance by controlling chloride homeostasis.

关键词： chloride efflux transporter DTX/MATE grain size rice salt tolerance

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A transceptor-channel complex couples nitrate sensing to calcium signaling in Ambidopsis

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Molecular Plant 2021年第5期14卷 774-786页

作者： Xiaohan Wang Changxin Feng lili Tian Congcong Hou Wang Tian Bin Hu Qian Zhang Zhijie Ren Qi Niu Jiali Song Dongdong Kong liangyu liu Yikun He ligeng Ma Chengcai Chu Sheng Luan legong li College of life Sciences Capital Normal UniversityBeijing 100048China State Key Laboratory of Plant Genomics Institute of Genetics and Developmental Biologythe Innovative Academy for Seed DesignChinese Academy of SciencesBeijing 100101China Department of Plant and Microbial Biology University of California at BerkeleyBerkeleyCA 94720USA

Nitrate-induced Ca^(2+) signaling is crucial for the primary nitrate response in plants.However,the molecular mechanism underlying the generation of the nitrate-specific calcium signature remains unknown.We report here that a cyclic nucleotide-gated channel(CNGC)protein,CNGC15,and the nitrate transceptor(NRT1.1)constitute a molecular switch that controls calcium influx depending on nitrate levels.The expression of CNGC15 is induced by nitrate,and its protein is localized at the plasma membrane after establishment of young seedlings.We found that disruption of CNGC15 results in the loss of the nitrate-induced Ca^(2+) signature(primary nitrate response)and retards root growth,reminiscent of the phenotype observed in the nrt1.1 mutant.We further showed that CNGC15 is an active Ca^(2+)-permeable channel that physically interacts with the NRT1.1 protein in the plasma membrane.Importantly,we discovered that CNGC15-NRT1.1 interaction silences the channel activity of the heterocomplex,which dissociates upon a rise in nitrate levels,leading to reactivation of the CNGC15 channel.The dynamic interactions between CNGC15 and NRT1.1 therefore control the channel activity and Ca^(2+) influx in a nitrate-dependent manner.Our study reveals a new nutrient-sensing mechanism that utilizes a nutrient transceptor-channel complex assembly to couple nutrient status to a specific Ca^(2+) signature.

关键词： primary nitrate response nutrient sensing calcium signature nitrate transceptor calcium channel

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Modulation of nitrate-induced phosphate response by the MYB transcription factor Rli1/HINGE1 in the nucleus

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Molecular Plant 2021年第3期14卷 517-529页

作者： Zhihua Zhang Zhao li Wei Wang Zhimin Jiang liping Guo Xiaohan Wang Yangwen Qian Xiahe Huang Yongqiang liu Xiujie liu Yahong liu Aifu li Yu Yan Junpeng Xie Shouyun Cao Stanislav Kopriva legong li Fanjiang Kong Baohui liu Yingchun Wang Bin Hu Chengcai Chu State Key Laboratory of Plant Genomics Institute of Genetics and Developmental Biologythe Innovative Academy for Seed DesignChinese Academy of SciencesBeijingChina School of life Sciences Guangzhou University.GuangzhouChina College of Plant Science Jilin UniversityChangchunChina Biogle Genome Editing Center ChangzhouChina State Key Laboratory of Molecular Developmental Biology Institute of Genetics and Developmental Biologythe Innovative Academy for Seed DesignChinese Academy of SciencesBeijingChina College of Advanced Agricultural Science University of Chinese Academy of SciencesBeijingChina Botanical Institute Cluster of Excellence on Plant SciencesUniversity of CologneCologneGermany College of life Sciences Capital Normal UniversityBeijingChina

The coordinated utilization of nitrogen(N)and phosphorus(P)is vital for plants to maintain nutrient balance and achieve optimal growth.Previously,we revealed a mechanism by which nitrate induces genes for phosphate utilization;this mechanism depends on NRT1.1B-facilitated degradation of cytoplasmic SPX4,which in turn promotes cytoplasmic-nuclear shuttling of PHR2,the central transcription factor of phosphate signaling,and triggers the nitrate-induced phosphate response(NIPR)and N-P coordinated utilization in rice.In this study,we unveiled a fine-tuning mechanism of NIPR in the nucleus regulated by Highly Induced by Nitrate Gene 1(HINGE1,also known as Rli1),a MYB-transcription factor closely related to PHR2.Rli1/HINGE1,which is transcriptionally activated by PHR2 under nitrate induction,can directly activate the expression of phosphate starvation-induced genes.More importantly,Rli1/HINGE1 competes with PHR2 for binding to its repressor proteins in the nucleus(SPX proteins),and consequently releases PHR2 to further enhance phosphate response.Therefore,Rli1/HINGE1 amplifies the phosphate response in the nucleus downstream of the cytoplasmic SPX4-PHR2 cascade,thereby enabling fine-tuning of N-P balance when nitrate supply is sufficient.

关键词： rice NIPR Rli1/HINGE1 PHR2 SPXs nucleus

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